Objective:To determine the prevalence of sub-assemblages of Giardia duodenalis(G.duodenalis)causing infection in preschool children in a central region of Cuba.Methods:A cross-sectional study was conducted on 417 chil...Objective:To determine the prevalence of sub-assemblages of Giardia duodenalis(G.duodenalis)causing infection in preschool children in a central region of Cuba.Methods:A cross-sectional study was conducted on 417 children from Sancti Spiritus from January to June 2013.A PCR amplification of the glutamate dehydrogenase(gdh)gene was performed for all positive samples in direct microscopic examination to enable sub-assemblages identification.Results:The prevalence of G.duodenalis in preschool children was 10.8%.DNA from 39 of 45(86.7%)samples was successfully amplified by PCR-restriction fragment length polymorphism analysis of the gdh gene.Restriction fragment length polymorphism analysis classified the 39 gdh amplicons in sub-assemblages BIII(11,28.2%),AII(8,20.5%)and BIV(4,10.3%).Mixed infections accounted for 41%,with the pattern AII/BIII(12,30.7%)being the predominant.Abdominal pain was statistically associated with infection with sub-assemblage BIII.Conclusions:Sub-assemblage BIII was the most commonly identified in the population of children studied,and significantly associated with abdominal pain in the symptomatic children.More defining tools to discriminate sub-assemblage and genotype levels to correlate with clinical data are needed in further studies.Large studies in endemic settings are required to elucidate the role that assemblage types play in G.duodenalis infections in vulnerable populations,such as children.展开更多
基金Supported by Swiss National Science Foundation(Grant No.31003A_138353)in part.
文摘Objective:To determine the prevalence of sub-assemblages of Giardia duodenalis(G.duodenalis)causing infection in preschool children in a central region of Cuba.Methods:A cross-sectional study was conducted on 417 children from Sancti Spiritus from January to June 2013.A PCR amplification of the glutamate dehydrogenase(gdh)gene was performed for all positive samples in direct microscopic examination to enable sub-assemblages identification.Results:The prevalence of G.duodenalis in preschool children was 10.8%.DNA from 39 of 45(86.7%)samples was successfully amplified by PCR-restriction fragment length polymorphism analysis of the gdh gene.Restriction fragment length polymorphism analysis classified the 39 gdh amplicons in sub-assemblages BIII(11,28.2%),AII(8,20.5%)and BIV(4,10.3%).Mixed infections accounted for 41%,with the pattern AII/BIII(12,30.7%)being the predominant.Abdominal pain was statistically associated with infection with sub-assemblage BIII.Conclusions:Sub-assemblage BIII was the most commonly identified in the population of children studied,and significantly associated with abdominal pain in the symptomatic children.More defining tools to discriminate sub-assemblage and genotype levels to correlate with clinical data are needed in further studies.Large studies in endemic settings are required to elucidate the role that assemblage types play in G.duodenalis infections in vulnerable populations,such as children.
