Objective: To explore the roles of the expression of the co-stimulatory molecule, B7-2, and the co-inhibitory molecule, PD-L1, on peripheral blood mononuclear cells in the mechanism of immunotolerance in chronic hepa...Objective: To explore the roles of the expression of the co-stimulatory molecule, B7-2, and the co-inhibitory molecule, PD-L1, on peripheral blood mononuclear cells in the mechanism of immunotolerance in chronic hepatitis B virus infection. Methods: Thirty HBV infected patients in the immunoreactive phase and 20 patients in the immunotolerant phase were enrolled in the study, while 20 healthy volunteers were used as controls. RT- PCR and real-time PCR methods were used to detect the expression levels of B7-2 and PD-L1 mRNA in peripheral blood mononuclear cells in chronic HBV infected patients. Results: The B7-2 expression in irnrnunoreactive and immunotolerant patients was significantly lower than that in the controls (P all 〈 0.01 ); B7-2 expression in immunoreactive patients was significantly lower than in immunotolerant patients (P 〈 0.01). PD-L1 expression in irnmunoreactive patients and immunotolerant patients was significantly higher than that in normal controls (P all 〈 0.01). The PD-L1/BT-2 ratios in immunoreactive and immunotolerant patients were significantly higher than that of the healthy controls (P all 〈 0.01); the PD-L1/ B7-2 ratio was significantly higher in the immunoreactive patients than in the immunotolerant patients (P 〈 0.01). Conclusion: In chronic HBV infection, changes in the expression of co-stimulatory and co-inhibitory molecules imply a protective adjustment against the patient' s immune response that may result in increased immunotolerance and persistent HBV infection.展开更多
程序性细胞死亡因子1(programmed cell death 1,PD-1)是一种共刺激分子,属于CD28家族,呈诱导性表达于活化的T细胞、B细胞和NK细胞表面,与其配体作用传递抑制性信号,发挥负向调控作用,这一信号通路在自身免疫性疾病、肿瘤发生、慢性病毒...程序性细胞死亡因子1(programmed cell death 1,PD-1)是一种共刺激分子,属于CD28家族,呈诱导性表达于活化的T细胞、B细胞和NK细胞表面,与其配体作用传递抑制性信号,发挥负向调控作用,这一信号通路在自身免疫性疾病、肿瘤发生、慢性病毒感染中具有重要的生物学意义。通过研究PD-1及其配体与临床疾病的相关性,阐明其作用机理后,利用信号调节可以达到维持自身耐受、抑制肿瘤、抗病毒和提高机体免疫应答等免疫治疗的目的。本文对PD-1及其配体的生物学功能,免疫治疗等方面的进展进行回顾。展开更多
文摘Objective: To explore the roles of the expression of the co-stimulatory molecule, B7-2, and the co-inhibitory molecule, PD-L1, on peripheral blood mononuclear cells in the mechanism of immunotolerance in chronic hepatitis B virus infection. Methods: Thirty HBV infected patients in the immunoreactive phase and 20 patients in the immunotolerant phase were enrolled in the study, while 20 healthy volunteers were used as controls. RT- PCR and real-time PCR methods were used to detect the expression levels of B7-2 and PD-L1 mRNA in peripheral blood mononuclear cells in chronic HBV infected patients. Results: The B7-2 expression in irnrnunoreactive and immunotolerant patients was significantly lower than that in the controls (P all 〈 0.01 ); B7-2 expression in immunoreactive patients was significantly lower than in immunotolerant patients (P 〈 0.01). PD-L1 expression in irnmunoreactive patients and immunotolerant patients was significantly higher than that in normal controls (P all 〈 0.01). The PD-L1/BT-2 ratios in immunoreactive and immunotolerant patients were significantly higher than that of the healthy controls (P all 〈 0.01); the PD-L1/ B7-2 ratio was significantly higher in the immunoreactive patients than in the immunotolerant patients (P 〈 0.01). Conclusion: In chronic HBV infection, changes in the expression of co-stimulatory and co-inhibitory molecules imply a protective adjustment against the patient' s immune response that may result in increased immunotolerance and persistent HBV infection.
文摘程序性细胞死亡因子1(programmed cell death 1,PD-1)是一种共刺激分子,属于CD28家族,呈诱导性表达于活化的T细胞、B细胞和NK细胞表面,与其配体作用传递抑制性信号,发挥负向调控作用,这一信号通路在自身免疫性疾病、肿瘤发生、慢性病毒感染中具有重要的生物学意义。通过研究PD-1及其配体与临床疾病的相关性,阐明其作用机理后,利用信号调节可以达到维持自身耐受、抑制肿瘤、抗病毒和提高机体免疫应答等免疫治疗的目的。本文对PD-1及其配体的生物学功能,免疫治疗等方面的进展进行回顾。