Objective： To explore the roles of the expression of the co-stimulatory molecule, B7-2, and the co-inhibitory molecule, PD-L1, on peripheral blood mononuclear cells in the mechanism of immunotolerance in chronic hepa...Objective： To explore the roles of the expression of the co-stimulatory molecule, B7-2, and the co-inhibitory molecule, PD-L1, on peripheral blood mononuclear cells in the mechanism of immunotolerance in chronic hepatitis B virus infection. Methods： Thirty HBV infected patients in the immunoreactive phase and 20 patients in the immunotolerant phase were enrolled in the study, while 20 healthy volunteers were used as controls. RT- PCR and real-time PCR methods were used to detect the expression levels of B7-2 and PD-L1 mRNA in peripheral blood mononuclear cells in chronic HBV infected patients. Results： The B7-2 expression in irnrnunoreactive and immunotolerant patients was significantly lower than that in the controls （P all 〈 0.01 ）; B7-2 expression in immunoreactive patients was significantly lower than in immunotolerant patients （P 〈 0.01）. PD-L1 expression in irnmunoreactive patients and immunotolerant patients was significantly higher than that in normal controls （P all 〈 0.01）. The PD-L1/BT-2 ratios in immunoreactive and immunotolerant patients were significantly higher than that of the healthy controls （P all 〈 0.01）; the PD-L1/ B7-2 ratio was significantly higher in the immunoreactive patients than in the immunotolerant patients （P 〈 0.01）. Conclusion： In chronic HBV infection, changes in the expression of co-stimulatory and co-inhibitory molecules imply a protective adjustment against the patient＇ s immune response that may result in increased immunotolerance and persistent HBV infection.展开更多
程序性细胞死亡因子1(programmed cell death 1,PD-1)是一种共刺激分子,属于CD28家族,呈诱导性表达于活化的T细胞、B细胞和NK细胞表面,与其配体作用传递抑制性信号,发挥负向调控作用,这一信号通路在自身免疫性疾病、肿瘤发生、慢性病毒...程序性细胞死亡因子1(programmed cell death 1,PD-1)是一种共刺激分子,属于CD28家族,呈诱导性表达于活化的T细胞、B细胞和NK细胞表面,与其配体作用传递抑制性信号,发挥负向调控作用,这一信号通路在自身免疫性疾病、肿瘤发生、慢性病毒感染中具有重要的生物学意义。通过研究PD-1及其配体与临床疾病的相关性,阐明其作用机理后,利用信号调节可以达到维持自身耐受、抑制肿瘤、抗病毒和提高机体免疫应答等免疫治疗的目的。本文对PD-1及其配体的生物学功能,免疫治疗等方面的进展进行回顾。展开更多
文摘Objective： To explore the roles of the expression of the co-stimulatory molecule, B7-2, and the co-inhibitory molecule, PD-L1, on peripheral blood mononuclear cells in the mechanism of immunotolerance in chronic hepatitis B virus infection. Methods： Thirty HBV infected patients in the immunoreactive phase and 20 patients in the immunotolerant phase were enrolled in the study, while 20 healthy volunteers were used as controls. RT- PCR and real-time PCR methods were used to detect the expression levels of B7-2 and PD-L1 mRNA in peripheral blood mononuclear cells in chronic HBV infected patients. Results： The B7-2 expression in irnrnunoreactive and immunotolerant patients was significantly lower than that in the controls （P all 〈 0.01 ）; B7-2 expression in immunoreactive patients was significantly lower than in immunotolerant patients （P 〈 0.01）. PD-L1 expression in irnmunoreactive patients and immunotolerant patients was significantly higher than that in normal controls （P all 〈 0.01）. The PD-L1/BT-2 ratios in immunoreactive and immunotolerant patients were significantly higher than that of the healthy controls （P all 〈 0.01）; the PD-L1/ B7-2 ratio was significantly higher in the immunoreactive patients than in the immunotolerant patients （P 〈 0.01）. Conclusion： In chronic HBV infection, changes in the expression of co-stimulatory and co-inhibitory molecules imply a protective adjustment against the patient＇ s immune response that may result in increased immunotolerance and persistent HBV infection.
文摘程序性细胞死亡因子1(programmed cell death 1,PD-1)是一种共刺激分子,属于CD28家族,呈诱导性表达于活化的T细胞、B细胞和NK细胞表面,与其配体作用传递抑制性信号,发挥负向调控作用,这一信号通路在自身免疫性疾病、肿瘤发生、慢性病毒感染中具有重要的生物学意义。通过研究PD-1及其配体与临床疾病的相关性,阐明其作用机理后,利用信号调节可以达到维持自身耐受、抑制肿瘤、抗病毒和提高机体免疫应答等免疫治疗的目的。本文对PD-1及其配体的生物学功能,免疫治疗等方面的进展进行回顾。
