The in vitro degradation characteristic of the poly D, L-lactic acid （ PDLIA ）/ hydroxyapatite （ HA ） compound were investigated. The compoundfibers were immersed in static phosphate buffer at 37℃ to degrade fo...The in vitro degradation characteristic of the poly D, L-lactic acid （ PDLIA ）/ hydroxyapatite （ HA ） compound were investigated. The compoundfibers were immersed in static phosphate buffer at 37℃ to degrade for 22 weeks. The changes in pH value of the buffer solution, the mechanical strength and morphological of inside and outside of composite fibers with degrurlation characteristic were observed. Results show that pH value of the buffer solution stabilized to aboat 7.0 before 12 weeks, however after 20 weeks that pH value quick declined. After 7 weeks that composite fibers of mechanical strength cannot mensuration. SEM observation revealed ttua bimodal degradation occurred in composite fibers.展开更多
Objective: To evaluate the osteocompatibility of D, L-polylactic/hydroxyapatite/decalcifying bone matrix (PDLLA/HA/DBM), and compare with PDLLA and DBM. Methods: Human primary osteoblasts isolated from the femoral hea...Objective: To evaluate the osteocompatibility of D, L-polylactic/hydroxyapatite/decalcifying bone matrix (PDLLA/HA/DBM), and compare with PDLLA and DBM. Methods: Human primary osteoblasts isolated from the femoral head of patients were inoculated onto PDLLA/HA/DBM, PLA and DBM respectively. The proliferation rate and collagen Ⅰ expression were detected. The interface between biomaterial and osteoblasts was investigated with phase contrast microscopy and electron scanning microscopy. Results: Best proliferation rate was observed with the PDLLA/HA/DBM and followed by DBM and PLA, suggesting that PDLLA/HA/DBM satisfying most requirements for the cultivation of human osteoblasts. Scanning electron microscopy showed the morphology of osteoblasts was correlated with the proliferation data. The cells, well spread and flattened, were attached closely on the surface of biomaterial with an arched structure and had normal morphology. The extracellular collagenous matrixs covered the surface of biomaterial and packed the granules of biomaterial. Conclusion: PDLLA/HA/DBM can form osteointerface early and have a good biocompability.展开更多
目的:探讨跟骨钛钢板和羟基磷灰石人工骨植骨治疗跟骨骨折的临床疗效。方法:对26例37足跟骨骨折行跟骨钛钢板开放复位内固定和植入羟基磷灰石人工骨治疗。按Sander分型:其中Ⅲ型28足,Ⅳ型9足。采用Maryl and Foot Score系统进行术后功...目的:探讨跟骨钛钢板和羟基磷灰石人工骨植骨治疗跟骨骨折的临床疗效。方法:对26例37足跟骨骨折行跟骨钛钢板开放复位内固定和植入羟基磷灰石人工骨治疗。按Sander分型:其中Ⅲ型28足,Ⅳ型9足。采用Maryl and Foot Score系统进行术后功能评价。结果:26例术后均获随访,时间7～42个月,平均11.8个月。骨折全部愈合,其中优19足,良13足,可3足,差2足,优良率为86.4%。术后并发症:本组有1足局部皮缘坏死,1足发生腓肠皮神经损伤,2足切口延迟愈合。结论:跟骨钛钢板和羟基磷灰石人工骨植骨是治疗跟骨骨折的良好方法。展开更多
Aim. To investigate the bioactivity of the self- designed biodegradable osteosynthetic devices made of resorbable hydroxyapatite microparticles/ poly- DL- lactide (HA/PDLLA) composites. Method. Forty- three rabbits wi...Aim. To investigate the bioactivity of the self- designed biodegradable osteosynthetic devices made of resorbable hydroxyapatite microparticles/ poly- DL- lactide (HA/PDLLA) composites. Method. Forty- three rabbits with a transverse transcondylar osteotomy of the distal femur were fixed intramedullary by a HA/PDLLA rod, the duration of follow- up were 3， 6， 12, 24 and 36 weeks. Histological, scanning electron microscopic (SEM), energy dispersive X- ray (EDX) and biomechanical analyses were done. Results. Active new bone formation and direct bone- bonding were seen at the bone- implant interface. Generous apatite crystals deposited and grew on the surface of the composites at 3～ 6 weeks postoperation. The interfacial shear strength increased significantly. Conclusion. Through the incorporating of resorbable HA microparticles, specific bone- bonding and active osteogenic capacity is introduced. This kind of bioactivity, together with other properties such as sufficient mechanical strength, enhanced biocompatibility and radiopacity, which are intrinsically unobtainable in totally resorbable polymer/polymer systems, make the HA/PDLLA composites become a desirable material for the internal fixation of cancellous bone.展开更多
文摘The in vitro degradation characteristic of the poly D, L-lactic acid （ PDLIA ）/ hydroxyapatite （ HA ） compound were investigated. The compoundfibers were immersed in static phosphate buffer at 37℃ to degrade for 22 weeks. The changes in pH value of the buffer solution, the mechanical strength and morphological of inside and outside of composite fibers with degrurlation characteristic were observed. Results show that pH value of the buffer solution stabilized to aboat 7.0 before 12 weeks, however after 20 weeks that pH value quick declined. After 7 weeks that composite fibers of mechanical strength cannot mensuration. SEM observation revealed ttua bimodal degradation occurred in composite fibers.
文摘Objective: To evaluate the osteocompatibility of D, L-polylactic/hydroxyapatite/decalcifying bone matrix (PDLLA/HA/DBM), and compare with PDLLA and DBM. Methods: Human primary osteoblasts isolated from the femoral head of patients were inoculated onto PDLLA/HA/DBM, PLA and DBM respectively. The proliferation rate and collagen Ⅰ expression were detected. The interface between biomaterial and osteoblasts was investigated with phase contrast microscopy and electron scanning microscopy. Results: Best proliferation rate was observed with the PDLLA/HA/DBM and followed by DBM and PLA, suggesting that PDLLA/HA/DBM satisfying most requirements for the cultivation of human osteoblasts. Scanning electron microscopy showed the morphology of osteoblasts was correlated with the proliferation data. The cells, well spread and flattened, were attached closely on the surface of biomaterial with an arched structure and had normal morphology. The extracellular collagenous matrixs covered the surface of biomaterial and packed the granules of biomaterial. Conclusion: PDLLA/HA/DBM can form osteointerface early and have a good biocompability.
文摘目的:探讨跟骨钛钢板和羟基磷灰石人工骨植骨治疗跟骨骨折的临床疗效。方法:对26例37足跟骨骨折行跟骨钛钢板开放复位内固定和植入羟基磷灰石人工骨治疗。按Sander分型:其中Ⅲ型28足,Ⅳ型9足。采用Maryl and Foot Score系统进行术后功能评价。结果:26例术后均获随访,时间7～42个月,平均11.8个月。骨折全部愈合,其中优19足,良13足,可3足,差2足,优良率为86.4%。术后并发症:本组有1足局部皮缘坏死,1足发生腓肠皮神经损伤,2足切口延迟愈合。结论:跟骨钛钢板和羟基磷灰石人工骨植骨是治疗跟骨骨折的良好方法。
基金This project was supported by the National Scientific Committee(969202011), the Natural Science Foundation of Hubei Province(99J
文摘Aim. To investigate the bioactivity of the self- designed biodegradable osteosynthetic devices made of resorbable hydroxyapatite microparticles/ poly- DL- lactide (HA/PDLLA) composites. Method. Forty- three rabbits with a transverse transcondylar osteotomy of the distal femur were fixed intramedullary by a HA/PDLLA rod, the duration of follow- up were 3， 6， 12, 24 and 36 weeks. Histological, scanning electron microscopic (SEM), energy dispersive X- ray (EDX) and biomechanical analyses were done. Results. Active new bone formation and direct bone- bonding were seen at the bone- implant interface. Generous apatite crystals deposited and grew on the surface of the composites at 3～ 6 weeks postoperation. The interfacial shear strength increased significantly. Conclusion. Through the incorporating of resorbable HA microparticles, specific bone- bonding and active osteogenic capacity is introduced. This kind of bioactivity, together with other properties such as sufficient mechanical strength, enhanced biocompatibility and radiopacity, which are intrinsically unobtainable in totally resorbable polymer/polymer systems, make the HA/PDLLA composites become a desirable material for the internal fixation of cancellous bone.
