目的:为提高非诺贝特溶解度,将非诺贝特包载于PEG_(2000)-DSPE胶束中,研究其在SD大鼠体内的口服药动学情况。方法:对非诺贝特PEG_(2000)-DSPE胶束进行表征,大鼠单剂量灌胃给予非诺贝特PEG_(2000)-DSPE胶束和非诺贝特混悬液,眼底静脉丛取...目的:为提高非诺贝特溶解度,将非诺贝特包载于PEG_(2000)-DSPE胶束中,研究其在SD大鼠体内的口服药动学情况。方法:对非诺贝特PEG_(2000)-DSPE胶束进行表征,大鼠单剂量灌胃给予非诺贝特PEG_(2000)-DSPE胶束和非诺贝特混悬液,眼底静脉丛取血,HPLC法测定血浆中非诺贝特酸含量,并用药物与统计(Drug and Statistics,DAS)软件分析处理药动学数据。结果:成功制备了非诺贝特PEG_(2000)-DSPE胶束,平均粒径为(23.40±3.62)nm,包封率和载药量分别为(97.65±3.32)%和(1.33±0.32)%。大鼠体内口服药动学结果表明非诺贝特PEG_(2000)-DSPE胶束和非诺贝特混悬液的药动学行为均符合二室模型,非诺贝特PEG20 00-DSPE胶束和非诺贝特混悬液的AUC_((0-24))分别为(61.41±5.71)μg·h·ml^(-1)和(8.49±0.66)μg·h·ml^(-1),C_(max)分别为(9.67±1.65)μg·ml^(-1)和(0.71±0.09)μg·ml^(-1)。非诺贝特PEG_(2000)-DSPE胶束的AUC_((0-24))和C_(max)相比于非诺贝特混悬液组分别提高了7倍和14倍。非诺贝特PEG_(2000)-DSPE胶束相对于原料药生物利用度为723.3%。结论:非诺贝特PEG_(2000)-DSPE胶束显著提高了非诺贝特在大鼠体内的口服吸收速度和生物利用度。PEG_(2000)-DSPE胶束作为口服药物载体具有优良的应用前景。展开更多
A mixed culture of bioleaching microorganisms was enriched.Then the mixed culture was preserved by liquid nitrogen cryopreservation for 6 months and 12 months,respectively,using PEG-2000 as the protective agent.The ch...A mixed culture of bioleaching microorganisms was enriched.Then the mixed culture was preserved by liquid nitrogen cryopreservation for 6 months and 12 months,respectively,using PEG-2000 as the protective agent.The chalcopyrite leaching ability,activity and diversity of the mixed culture before and after preservation were compared.The results showed that the copper extraction rate was 95.7%in chalcopyrite bioleaching within 20 d by the original culture.After cryopreservation for 6 months and 12 months,the copper extraction rate of the mixed culture was 94.9%within 25 d and 93.6%within 35 d,respectively.The cell viability achieved 87%and 41%after being preserved for 6 months and 12 months,respectively.Furthermore,the ecology analysis identified Acidithiobacillus ferrooxidans,Acidithiobacillus caldus,Sulfobacillus thermotolerans and Pseudomonas aeruginosa in the original mixed culture.After cryopreservation for 12 months,the composition of community changed,but the predominant microorganisms still existed.展开更多
文摘目的:为提高非诺贝特溶解度,将非诺贝特包载于PEG_(2000)-DSPE胶束中,研究其在SD大鼠体内的口服药动学情况。方法:对非诺贝特PEG_(2000)-DSPE胶束进行表征,大鼠单剂量灌胃给予非诺贝特PEG_(2000)-DSPE胶束和非诺贝特混悬液,眼底静脉丛取血,HPLC法测定血浆中非诺贝特酸含量,并用药物与统计(Drug and Statistics,DAS)软件分析处理药动学数据。结果:成功制备了非诺贝特PEG_(2000)-DSPE胶束,平均粒径为(23.40±3.62)nm,包封率和载药量分别为(97.65±3.32)%和(1.33±0.32)%。大鼠体内口服药动学结果表明非诺贝特PEG_(2000)-DSPE胶束和非诺贝特混悬液的药动学行为均符合二室模型,非诺贝特PEG20 00-DSPE胶束和非诺贝特混悬液的AUC_((0-24))分别为(61.41±5.71)μg·h·ml^(-1)和(8.49±0.66)μg·h·ml^(-1),C_(max)分别为(9.67±1.65)μg·ml^(-1)和(0.71±0.09)μg·ml^(-1)。非诺贝特PEG_(2000)-DSPE胶束的AUC_((0-24))和C_(max)相比于非诺贝特混悬液组分别提高了7倍和14倍。非诺贝特PEG_(2000)-DSPE胶束相对于原料药生物利用度为723.3%。结论:非诺贝特PEG_(2000)-DSPE胶束显著提高了非诺贝特在大鼠体内的口服吸收速度和生物利用度。PEG_(2000)-DSPE胶束作为口服药物载体具有优良的应用前景。
基金Projects(31470230,51320105006,51604308)supported by the National Natural Science Foundation of ChinaProject(2017RS3003)supported by the Youth Talent Foundation of Hunan Province of China+2 种基金Project(2018JJ2486)supported by the Natural Science Foundation of Hunan Province of ChinaProject(2018WK2012)supported by the Key Research and Development Projects in Hunan Province,ChinaProject(2018zzts767)supported by the Fundamental Research Funds for the Central Universities,China。
文摘A mixed culture of bioleaching microorganisms was enriched.Then the mixed culture was preserved by liquid nitrogen cryopreservation for 6 months and 12 months,respectively,using PEG-2000 as the protective agent.The chalcopyrite leaching ability,activity and diversity of the mixed culture before and after preservation were compared.The results showed that the copper extraction rate was 95.7%in chalcopyrite bioleaching within 20 d by the original culture.After cryopreservation for 6 months and 12 months,the copper extraction rate of the mixed culture was 94.9%within 25 d and 93.6%within 35 d,respectively.The cell viability achieved 87%and 41%after being preserved for 6 months and 12 months,respectively.Furthermore,the ecology analysis identified Acidithiobacillus ferrooxidans,Acidithiobacillus caldus,Sulfobacillus thermotolerans and Pseudomonas aeruginosa in the original mixed culture.After cryopreservation for 12 months,the composition of community changed,but the predominant microorganisms still existed.
