目的探讨PHF2在乳腺癌细胞和组织中的表达及其与乳腺癌临床病理特征、预后的关系。方法采用q RT-PCR法检测乳腺癌及正常乳腺上皮细胞株(n=7)及配对的癌旁组织(n=30)中PHF2 m RNA的表达水平;应用Western blot法检测乳腺癌细胞株中PHF2蛋...目的探讨PHF2在乳腺癌细胞和组织中的表达及其与乳腺癌临床病理特征、预后的关系。方法采用q RT-PCR法检测乳腺癌及正常乳腺上皮细胞株(n=7)及配对的癌旁组织(n=30)中PHF2 m RNA的表达水平;应用Western blot法检测乳腺癌细胞株中PHF2蛋白的表达水平,免疫组化法检测乳腺癌组织(n=50)和癌旁组织(n=15)中PHF2蛋白的表达,分析PHF2表达与乳腺癌临床病理特征的关系及预后的关系。结果与正常人乳腺上皮细胞相比,PHF2 m RNA和蛋白在乳腺癌细胞株中表达下调(P<0.01);与癌旁组织相比,PHF2 m RNA在90%(27/30)的乳腺癌组织中表达下调(P<0.05);免疫组化结果显示:在70%(35/50)的乳腺癌组织中PHF2蛋白表达下调(P<0.05);PHF2表达下调与乳腺癌患者的淋巴结转移、ER阴性及预后密切相关(P<0.05)。结论 PHF2在乳腺癌中表达下调,可以作为乳腺癌预后判断的指标之一,有望成为乳腺癌治疗的新靶点。展开更多
Genes encoding proteins with PHD (plant homeodomain) finger motif (C4HC3) are highly conserved from Arabidopsis to Homo sapiens. One of the major functions of these genes is regulating the expression of homeotic genes...Genes encoding proteins with PHD (plant homeodomain) finger motif (C4HC3) are highly conserved from Arabidopsis to Homo sapiens. One of the major functions of these genes is regulating the expression of homeotic genes during the stage of embryonic development. They play a role in cell-cycle and cell differentiation and seem to be related with some human malignant diseases, such as leukemia. A human placenta cDNA library has been screened with cDNA probe amplified by PCR. The PGR primers have been designed according to the M96 (a mouse gene encoding a protein with PHD domain) homologous data in dbEST.A 2. 1 kb insert fragment in a positive cDNA clone has been isolated and sequenced. This new full-length cDNA is named PHF2 (GenBank Acc: AF052205). The putative protein composed of 567 aa has two typical PHD fingers at its N-terminus. Meanwhile it is identified that there are several alternatively spliced transcripts of PHF2 in different human tissues through the PCR amplification, Northern blot展开更多
文摘目的探讨PHF2在乳腺癌细胞和组织中的表达及其与乳腺癌临床病理特征、预后的关系。方法采用q RT-PCR法检测乳腺癌及正常乳腺上皮细胞株(n=7)及配对的癌旁组织(n=30)中PHF2 m RNA的表达水平;应用Western blot法检测乳腺癌细胞株中PHF2蛋白的表达水平,免疫组化法检测乳腺癌组织(n=50)和癌旁组织(n=15)中PHF2蛋白的表达,分析PHF2表达与乳腺癌临床病理特征的关系及预后的关系。结果与正常人乳腺上皮细胞相比,PHF2 m RNA和蛋白在乳腺癌细胞株中表达下调(P<0.01);与癌旁组织相比,PHF2 m RNA在90%(27/30)的乳腺癌组织中表达下调(P<0.05);免疫组化结果显示:在70%(35/50)的乳腺癌组织中PHF2蛋白表达下调(P<0.05);PHF2表达下调与乳腺癌患者的淋巴结转移、ER阴性及预后密切相关(P<0.05)。结论 PHF2在乳腺癌中表达下调,可以作为乳腺癌预后判断的指标之一,有望成为乳腺癌治疗的新靶点。
文摘Genes encoding proteins with PHD (plant homeodomain) finger motif (C4HC3) are highly conserved from Arabidopsis to Homo sapiens. One of the major functions of these genes is regulating the expression of homeotic genes during the stage of embryonic development. They play a role in cell-cycle and cell differentiation and seem to be related with some human malignant diseases, such as leukemia. A human placenta cDNA library has been screened with cDNA probe amplified by PCR. The PGR primers have been designed according to the M96 (a mouse gene encoding a protein with PHD domain) homologous data in dbEST.A 2. 1 kb insert fragment in a positive cDNA clone has been isolated and sequenced. This new full-length cDNA is named PHF2 (GenBank Acc: AF052205). The putative protein composed of 567 aa has two typical PHD fingers at its N-terminus. Meanwhile it is identified that there are several alternatively spliced transcripts of PHF2 in different human tissues through the PCR amplification, Northern blot
