基于5G多接入边缘计算的云化PLC系统架构设计与应用

随着工业控制系统对柔性化、扁平化的要求不断提升,以PLC为构建基础的传统工业控制网络在大规模接入、算力提升和部署灵活性方面均受到限制.针对这些问题,融合5G网络和边缘计算技术,设计了基于5G多接入边缘计算的云化PLC系统架构,阐述了该架构的运行机制和所涉及的关键技术;在此基础上,进行PLC系统的云化部署和调试,实现了工业现场、企业数据中心、5G网络和边缘网络的集成;最后,通过在某汽车制造公司产线改造过程中的应用,验证了所提架构的有效性.

基于5G+MEC的PLC架构设计与工业制造应用探索

工业控制是工业生产的核心环节,是实现大规模自动化生产的基础。5G网络与制造体系的深度融合创新,推动了新型工业化的新工业革命。PLC是现代工业控制系统的典型代表,5G+PLC是工业控制系统智能化变革的关键切入点。PLC是工业自动化的核心组成部分,5G与PLC的融合将实现组网架构、部署形式、关键功能的变化,实现更便捷、更经济、更可靠的工业控制。文中探索了5G+PLC的实践,给出了5G+PLC赋能行业数字化转型的解决方案,提出了不同控制模型下对5G网络的性能要求及网络部署方案。

基于4G/5G+PLC的远程门禁控制系统研究与运用

基于4G/5G数字通信网络和PLC技术,通过云端技术、DTU技术和SCADA软件实现有效远程控制,控制对象几乎可涵盖所有自动化执行对象。这样的控制方式,利用了既有的设备及基础的互联网,达到了对门禁设备远程监视控制的目标。

Association between 5-HTR1A gene C-1019G polymorphism and antidepressant response in patients with major depressive disorder:A meta-analysis

BACKGROUND Major depressive disorder(MDD)is a substantial global health concern,and its treatment is complicated by the variability in individual response to antide-pressants.AIM To consolidate research and clarify the impact of genetic variation on MDD treatment outcomes.METHODS Adhering to Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines,a systematic search across PubMed,EMBASE,Web of Science,and the Cochrane Library was conducted without date restrictions,utilizing key terms related to MDD,serotonin 1A receptor polymorphism(5-HTR1A),C-1019G polymorphism,and antidepressant response.Studies meeting inclusion criteria were thoroughly screened,and quality assessed using the Newcastle-Ottawa Scale.Statistical analyses,includingχ2 and I²values,were used to evaluate heterogeneity and fixed-effect or random-effect models were applied accordingly.RESULTS The initial search yielded 1216 articles,with 11 studies meeting criteria for inclusion.Analysis of various genetic models showed no significant association between the 5-HTR1A C-1019G polymorphism and antidepressant efficacy.The heterogeneity was low to moderate,and no publication bias was detected through funnel plot symmetry and Egger's and Begg's tests.CONCLUSION This meta-analysis does not support a significant association between the 5-HTR1A C-1019G polymorphism and the efficacy of antidepressant treatment in MDD.The findings call for further research with larger cohorts to substantiate these results and enhance the understanding of antidepressant pharmacogenetics.

Functional Studies of Castor(Ricinus communis L.)PLC Family Genes in Arabidopsis Inflorescence Development

Castor(Ricinus communis L.)is one of the top 10 oil crops in the world,and inflorescence is a trait that directly affects its yield.Phospholipase C(PLCs)is involved in many plant activities and metabolic processes.To study the functions of PLC family genes in the regulation of the inflorescence development of the female line of Lm-type castor aLmAB2,we determined the expression levels of six PLC family genes of three types of inflorescences of aLmAB2(isofemale line,female line,bisexual line)at different developmental stages.The results showed that the 6 genes of the castor PLC family had relative expression levels at different developmental stages of the three types of inflorescences.The subcellular location of all six protein products was the cell membrane.The six genes were heterologously overexpressed in Arabidopsis thaliana to obtain the T3 generation-resistant Arabidopsis thaliana plants.The results showed that the overexpression of six genes significantly promoted the maturation of Arabidopsis thaliana,the growth of lateral moss,and the development of flowers and pods,but the development of basal leaves and stem leaves of Arabidopsis thaliana was significantly inhibited.According to homology analysis,it is speculated that PLC2,PLC2M,PLC2N,PLC4,PLC4X2,and PLC6 genes have the same regulatory function.

基于GPRS和PLC的鸡舍环境监控系统设计

系统采用GPRS与PLCS7-200相结合,设计了一种鸡舍环境监控系统,介绍了系统的结构、软件设计和组态过程。监控系统根据鸡生长的环境条件,对环境参数进行实时采集,控制各种控制单元输出,从而给鸡创造最优的生长环境。使用组态软件设计的上位机监控系统实现了实时数据监控管理,保证信息在全范围内的畅通,以适应农业现代化的需要。

基于G蛋白偶联—PLC—IP3信号途径探讨湿阻脾胃证模型ICC Ca^(2+)调节机制及平胃散干预作用

目的探讨平胃散如何影响湿阻脾胃证模型ICC内、外Ca^(2+)浓度及G蛋白偶联—PLC—IP3信号途径,并揭示平胃散调节胃肠运动的作用机制。方法用湿阻脾胃证模型动物血清建立"湿阻脾胃证ICC模型"。动物血清的制备:采用本实验室建立的湿阻脾胃证动物模型方法造模,造模20天后,分别取正常组动物血清,湿阻模型组动物血清,给药组动物血清及自然恢复组血清。用Ⅱ胶原酶消化法体外培养小鼠小肠ICC,经形态学观察和免疫荧光学鉴定成功后,通过细胞造模后血清给药,观察细胞内PLC活性,IP3、IP3R含量以及细胞内、外Ca^(2+)浓度变化。结果与正常组比较,模型组细胞内、外Ca^(2+)浓度水平均降低(P<0.05),PLC活性升高(P<0.05),IP3含量无显著性差异,IP3R含量降低(P<0.05);与正常组比较,自然恢复组细胞内、外Ca^(2+)浓度水平均无显著性差异,PLC活性升高(P<0.05),IP3含量无显著性差异,IP3R含量升高(P<0.05);与模型组比较,自然恢复组细胞内、外Ca^(2+)浓度水平均升高(P<0.05),PLC活性升高(P<0.05),IP3含量无显著性差异,IP3R含量升高;平胃散组细胞内、外Ca^(2+)浓度水平均升高(P<0.05),PLC活性大幅升高(P<0.05),IP3含量升高(P<0.05),IP3R含量升高(P<0.05)。结论平胃散可能是通过影响G蛋白—PLC—IP3信号途径,使ICC细胞内Ca^(2+)浓度周期性震荡恢复,产生起搏电位,而起到调节胃肠运动。

基于3G通讯和PLC的交通信号控制系统

设计的信号控制系统是一种基于3G通讯和PLC组合控制的二进制指挥系统。在现有交通控制基础上改造控制端,给出了二进制指挥系统(信号灯)的软硬件设计与实现方法,并结合仿真软件进行试验分析。同时搭建了一套可监控、智能疏导的资源利用交通平台,解决了路况检修不及时、车辆占道等多种潜在问题。经分析,与传统的控制方法相比,该方法提高了交叉口的通行能力,从而减少交通延误,具有一定的应用前景。

浅析S7-1200PLC与G120变频器的自由报文格式通信

介绍S7-1200PLC以太网的自由报文通信方式,控制G120变频器,实现对电机的转速控制,电机的速度设定值和运行的状态参数通过HMI输入和显示,采用TIA Portal V12开发平台,提高了设计效率。

OMRON CS1G PLC在直线式悬臂电镀自动控制的应用

介绍了OMRONCS1G系列PLC在直线式悬臂电镀自动控制中的应用 ,阐述了直线式悬臂电镀自动控制系统的组成和工作原理 ,叙述了OMRONCS1G系列PLC与触摸屏结合的控制方式 ,结合直线式悬臂电镀自动控制系统的特点 ,给出了系统的软硬件结构设计。该控制系统的控制精度和可靠运行等性能达到满意的控制效果。实际运行情况表明 ,系统运行稳定 。

S1500 PLC和G120变频器在牵引喂入机中的应用

介绍了S1500PLC和G120变频器组成的控制系统,通过交流供电直流共母线方式在涤纶短纤前纺牵引喂入机中的应用。

SB70G变频器PLC控制技术

SB70G的PLC可选择多个模式,相当于具有多套简易PLC设置,可通过切换不同的模式来达到不同规格产品的生产工艺要求,水泥管桩离心制造设备可以选择不同模式生产不同规格的管桩。生产6种规格的管桩,每种规格需8段PLC运行,可设置F8-01个位=4,运行中切换模式在停机后生效,可选择的最大模式由F8-01个位决定。

Molecular Characterization of Viral G Gene in Emerging and Re-emerging Areas of Rabies in China, 2007 to 2011

In recent years (2007 to 2011), although the overall number of rabies cases in China has decreased, there is evidence of emerging or re-emerging cases in regions without previous rabies cases or with low incidence of rabies. To investigate the origin and the factors affecting the spread of rabies in China, specimens were collected from 2007 to 2011 from provinces with emerging and re-emerging cases and tested for the presence of the rabies virus. Positive specimens were combined with sequences from GenBank to perform comparisons of homology and functional sites, and to carry out phylogenetic analyses. Out of these regions, five provinces had 9 positive specimens from canine and cattle, and 34 canine or human specimens were obtained from previously high-incidence provinces. Complete sequences of G gene were obtained for these samples. Homology of the sequences of these 43 specimens was 87%-100% at the nucleotide level and 93.7% -100% at the amino acid level. These G gene sequences were combined with reference sequence from GenBank and used to construct a phylogenetic tree. The results showed that 43 specimens were all assigned to China clade I and clade II, with all specimens from emerging and re-emerging areas placed within clade I. Specimens isolated from Shanxi and Inner Mongolia in 2011 were distinct from previously-isolated local strains and had closer homology to strains from Hebei, Beijing and Tianjin whereas new isolates from Shanghai were tightly clustered with strains isolated in the 1990s. Finally, Shaanxi isolates were clustered with strains from adjacent Sichuan. Our results suggest that the rabies cases in emerging and re-emerging areas in China in the last 5 years are a consequence of the epidemic spreading from of neighboring provinces and regions experiencing a serious epidemic of rabies.

Gene Cloning and Expression Analysis of G Protein αq Subunit from Helicoverpa assulta (Guenée)

The cDNA encoding the G protein αq subunit was isolated from the antennae of Helicoverpa assulta （Guen6e） by reverse transcription polymerase chain reaction （RT-PCR） and named as HassGαq. Sequencing analysis showed that the fulllength of HassGαq open reading frame （ORF） is 1 062 bp, 353 amino acid residues are encoded. The predicted molecular weights （MW） and isoelectric point （PI） are 41.5 kD and 5.15, respectively. HassGαq gene was then constructed into expression vector pGEX-4T-2 for over expression in prokaryotic cells. The SDS-PAGE and Western blot analysis showed that induced by Isopropyl-β-D-Thiogalactoside （IPTG）, the GST-HassGαq fusion protein is expressed in Escherichia coil BL21, and its MW was found to be about 66 kD nearly equal to the predicted. In addition, RT-PCR analysis showed that the expressions of HassGαq are not tissue specific.

E670G polymorphism of PCSK9 gene of patients with coronary heart disease among Han population in Hainan and three provinces in the northeast of China

Objective: To investigate the correlation between E670 G polymorphism of proprotein convertase subtilisin/kexin type 9(PCSK9) gene and coronary heart disease(CHD), and contrastively study the regional differences of E670 G polymorphism of PCSK9 gene between patients with CHD among the Han population in Hainan and three provinces in the northeast of China(TPNC), providing scientific basis for prevention and treatment of patients with CHD in different regions. Methods: A total of 233 cases of patients with CHD were selected from the Han population in Hainan and TPNC as the experimental group(118 cases from Hainan, 115 cases from TPNC), and 239 cases with non-CHD were selected among the Han population also in the two regions as control group(125 cases from Hainan, 114 cases from TPNC). The triglyceride(TG), total cholesterol(TC), high density lipoprotein cholesterol and low density lipoprotein cholesterol(LDL-C) levels of plasma were tested and PCR-RFLP method was used to test the E670 G polymorphism of PCSK9 gene. The statistical software package SPSS 21.0 was used for the statistical analysis and P<0.05 was considered as statistically significant. Results: The levels of systolic pressure, diastolic blood pressure, fasting blood sugar, TC, TG, and LDL-C of patients in CHD group were significantly higher than those in non-CHD group, while the high density lipoprotein cholesterol level was lower than that in non-CHD group(P<0.05). In CHD group, the frequencies of AG, GG genotypes of PCSK9 gene and G allele were higher than those in non-CHD group(P<0.05), and in CHD group, the frequencies of AG, GG genotypes and G allele of patients both in Hainan and TPNC were higher than those in control group(P<0.05). Among the patients with CHD, the frequencies of GG genotype and G allele of patients in Hainan were lower than those in TPNC(P<0.05), and in CHD group, the levels of TG, TC and LDL-C of GG genotype were higher than those of AA genotype(P<0.05). While in non-CHD group, there were no significant differences between the frequencies of GG genotype and G allele of patients in Hainan and TPNC(P>0.05). Conclusions: There was a close correlation between the E670 G polymorphism of PCSK9 gene and CHD with serum lipid level. Among Han population in Hainan and TPNC, the E670 G polymorphism of PCSK9 gene of patients with CHD exhibited regional differences.

Development of highly glyphosate-tolerant tobacco by coexpression of glyphosate acetyltransferase gat and EPSPS G2-aroA genes

The widely used herbicide glyphosate targets 5-enolpyruvylshikimate-3-phosphate synthase(EPSPS).Glyphosate acetyltransferase(GAT)effectively detoxifies glyphosate by N-acetylation.With the aim of identifying a new strategy for development of glyphosate-tolerant crops,the plant expression vector pG2-GAT harboring gat and G2-aroA(encoding EPSPS)has been transformed into tobacco(Nicotiana tabacum)to develop novel plants with higher tolerance to glyphosate.Results from Southern and Western blotting analyses indicated that the target genes were integrated into tobacco chromosomes and expressed effectively at the protein level.Glyphosate tolerance was compared among transgenic tobacco plants containing gat,G2-aroA,or both genes.Plants containing both gat and G2-aroA genes were the most glyphosate-tolerant.This study has shown that a combination of different strategies may result in higher tolerance in transgenic crops,providing a new approach for development of glyphosate-tolerant crops.

Relations of Budd-Chiari syndrome to prothrombin gene mutation

BACKGROUND: Budd-Chiari syndrome (BCS) is a type of disease characterized by portal hypertension and/or hy- pertension of the inferior vena cava (IVC) due to the ob- struction of the hepatic veins (HV) and/or intrahepatic IVC outlet. Being etiologically complicated and obscure, BCS can be acquired or idiopathic and several gene muta- tions may be contributable. This study was to explore whether prothrombin gene mutation (F G20210A) takes part in the pathogenesis of BCS and to investigate their cor- relativity. METHODS: In 38 proven BCS patients and 70 controls, polymerase chain reaction-restriction fragment length poly- morphism (PCR-RFLP) was used to find F G20210A mutation. To detect whether there are any mutations, four steps were taken: purification of genome DNA from whole blood, amplification of special fragment by polymerase chain reaction, digestion of the fragment via restriction en- donuclease, and analysis of results by polyacrylamide gel electrophoresis. RESULTS: F G20210A mutation was not detected in all patients and controls. CONCLUSIONS: No F G20210A mutation exists in Chi- nese patients with BCS, nor correlativity between the oc- currence of BCS and F G20210A mutation. The etiology of BCS in the Chinese needs further investigation.

基于3G通信的PLC嵌入式监控终端设计与实现

本文以欧姆龙CP1H型号的PLC为应用背景,设计了基于嵌入式linux下3G无线通信的PLC设备远程监控终端。论文针对GPRS与以太网的不足在实际应用的缺陷,提出了基于3G通信技术的数据传输方案,采用S3C6410/ARM11作为控制器和中兴MF210无线模块,采用WCDMA制式,通过在嵌入式linux系统中配置相关驱动,移植PPP套件,编写配置脚本来实现3G通信数据监控功能。介绍硬件设计,驱动移植,网络通信功能的实现过程。本方案具有快速稳定可靠等优点。

Characterization of reference genes for qPCR analysis in various tissues of the Fujian oyster Crassostrea angulata

Accurate quantification of transcripts using quantitative real-time polymerase chain reaction （qPCR） depends on the identification of reliable reference genes for normalization. This study aimed to identify and validate seven reference genes, including actin-2 （ACT-2）, elongation factor 1 alpha （EF-1α）, elongation factor 1 beta （EF-1β）, glyceraldehyde-3-phosphate dehydrogenase （GAPDH）, ubiquitin （UBQ）, β-tubulin （β-TUB）, and 18 S ribosomal RNA, from Crassostrea angulata, a valuable marine bivalve cultured worldwide. Transcript levels of the candidate reference genes were examined using qPCR analysis and showed differential expression patterns in the mantle, gill, adductor muscle, labial palp, visceral mass, hemolymph and gonad tissues. Quantitative data were analyzed using the geNorm software to assess the expression stability of the candidate reference genes, revealing that β-TUB and UBQ were the most stable genes. The commonly used GAPDH and 18S rRNA showed low stability, making them unsuitable candidates in this system. The expression pattern of the G protein β-subunit gene （Gβ） across tissue types was also examined and normalized to the expression of each or both of UBQ andβ-TUB as internal controls. This revealed consistent trends with all three normalization approaches, thus validating the reliability of UBQ and β-TUB as optimal internal controls. The study provides the first validated reference genes for accurate data normalization in transcript profiling in Crassostrea angulata, which will be indispensable for further fimetional genomics studies in this economically valuable marine bivalve.

基于GMR系统的PLC在F&G系统中的应用

火灾和气体保护系统(F&G)在消防、安全、工业过程控制等领域中已经得到了广泛的应用。采用GMR系统,设计基于PLC技术的F&G监控系统,利用PLC中以太网通讯模块实现了PLC和控制中心人机界面的通信,并使用组态软件编制上位机的控制界面,实现以PLC和组态软件构成的监控系统对油气田的消防自动监控和联动控制。系统达到了操作方便、性能稳定、自动化程度较高的控制效果。