将含Arg-Gly-Asp(RGD)肽对多孔的PLGA-[ASP-PEG]表面进行改性,然后置于仿体液中两周,探讨肽改性的PLGA-(PEG-ASP)在仿体液中的矿化情况。通过交联剂Sulfo-LC-SPDP把肽接枝到PLGA-[ASP-PEG],X射线光电子能谱仪(X-ray photoelectron spect...将含Arg-Gly-Asp(RGD)肽对多孔的PLGA-[ASP-PEG]表面进行改性,然后置于仿体液中两周,探讨肽改性的PLGA-(PEG-ASP)在仿体液中的矿化情况。通过交联剂Sulfo-LC-SPDP把肽接枝到PLGA-[ASP-PEG],X射线光电子能谱仪(X-ray photoelectron spectroscopy,XPS)鉴定;肽改性PLGA-[ASP-PEG]为实验组(Experiment group,EG),PLGA-[ASP-PEG]为对照组(Control group,CG),均置于仿体液中两周,扫描电子显微镜(Scanning electron microscope,SEM)、X射线能谱仪(Energy dispersive analysis system of X-ray,EDS)及X射线衍射仪(X-ray diffractometry,XRD)对矿化情况进行分析。XPS检测肽改性PLGA-[ASP-PEG]表面硫元素结合能为164eV,碳、硫元素的含量比值(C/S)为99.746∶0.1014;SEM示在EG表面及内部呈现连续的较为致密的矿化层,CG仅见分散稀疏的矿化层;EDS及XRD结果表明矿化物主要成分为羟基磷灰石,EG矿化物中钙/磷比值为1.60,CG矿化物中钙/磷比值为1.52。含RGD肽为矿化提供了丰富的功能基团,PLGA-(PEG-ASP)经肽修饰矿化后,获得了与天然骨基质相似的微观结构。展开更多
In this study, the bioactivity of a novel BMP2-derived oligopeptide P24 was investigated by using the model of rabbit femoral defect after loaded in the biodegradable poly （lactic acid / glycolic acid / asparagic aci...In this study, the bioactivity of a novel BMP2-derived oligopeptide P24 was investigated by using the model of rabbit femoral defect after loaded in the biodegradable poly （lactic acid / glycolic acid / asparagic acid-co-polyethylene glycol） （PLGA-[ASP-PEG]）. A 1.5-cm unilateral segmental bone defect was created in the left femoral diaphysis in each of the 30 new zealand white rabbits. The defects of 18 legs filled with BMP2-derived peptide P24 combined with PLGA-[ASP-PEG] scaffold serves as the experimental group, and the defects in the rest 12 rabbits filled with （PLGA-[ASP-PEG]） without P24 as control group. The bone-repairing capability in the target region of the two group was grossly, radiologically, histopathologically and biomechanically evaluated 4, 8 and 12 weeks after the operation. Our results showed that in each group, primary healing of incision was achieved in the two groups. Radiographically, in experimental group, defects were filled with induced callus within 8 weeks, and a cortical bone-like structure was observed in some animals at the 12th week. According to the standardized stage of bone defect repair, 9 （64.28%） achieved grade-4 healing. In contrast, little bone formation was seen in the defects even 12 weeks after the operation, and 5 （62.50%） had grade 0 healing in this group. Histologically, tissue engineering material was mostly absorbed and cartilage was found around implants in the experimental group at the 4th week; 8 weeks after operation, the engineering material was completely absorbed, and formation of woven bone was observed and typical trabecular bone structure could be seen. In control group, 8 weeks after operation, the defect was filled with fibrous tissues, and no bone-like structure was observed. Statistical analysis showed very significant difference in biomechanical indicators between the two groups （P〈0.05）. It is concluded that new oligopeptide P24 can induce excellent bone regeneration and promote bone repair.展开更多
文摘将含Arg-Gly-Asp(RGD)肽对多孔的PLGA-[ASP-PEG]表面进行改性,然后置于仿体液中两周,探讨肽改性的PLGA-(PEG-ASP)在仿体液中的矿化情况。通过交联剂Sulfo-LC-SPDP把肽接枝到PLGA-[ASP-PEG],X射线光电子能谱仪(X-ray photoelectron spectroscopy,XPS)鉴定;肽改性PLGA-[ASP-PEG]为实验组(Experiment group,EG),PLGA-[ASP-PEG]为对照组(Control group,CG),均置于仿体液中两周,扫描电子显微镜(Scanning electron microscope,SEM)、X射线能谱仪(Energy dispersive analysis system of X-ray,EDS)及X射线衍射仪(X-ray diffractometry,XRD)对矿化情况进行分析。XPS检测肽改性PLGA-[ASP-PEG]表面硫元素结合能为164eV,碳、硫元素的含量比值(C/S)为99.746∶0.1014;SEM示在EG表面及内部呈现连续的较为致密的矿化层,CG仅见分散稀疏的矿化层;EDS及XRD结果表明矿化物主要成分为羟基磷灰石,EG矿化物中钙/磷比值为1.60,CG矿化物中钙/磷比值为1.52。含RGD肽为矿化提供了丰富的功能基团,PLGA-(PEG-ASP)经肽修饰矿化后,获得了与天然骨基质相似的微观结构。
基金the National Natural Sciences Foundation of China (Nos. 30470483, 30200063, 30170270)a Program for New Century Excellent Talents (NCET) in University from the Ministry of Education of China (No. 20045006071-10)
文摘In this study, the bioactivity of a novel BMP2-derived oligopeptide P24 was investigated by using the model of rabbit femoral defect after loaded in the biodegradable poly （lactic acid / glycolic acid / asparagic acid-co-polyethylene glycol） （PLGA-[ASP-PEG]）. A 1.5-cm unilateral segmental bone defect was created in the left femoral diaphysis in each of the 30 new zealand white rabbits. The defects of 18 legs filled with BMP2-derived peptide P24 combined with PLGA-[ASP-PEG] scaffold serves as the experimental group, and the defects in the rest 12 rabbits filled with （PLGA-[ASP-PEG]） without P24 as control group. The bone-repairing capability in the target region of the two group was grossly, radiologically, histopathologically and biomechanically evaluated 4, 8 and 12 weeks after the operation. Our results showed that in each group, primary healing of incision was achieved in the two groups. Radiographically, in experimental group, defects were filled with induced callus within 8 weeks, and a cortical bone-like structure was observed in some animals at the 12th week. According to the standardized stage of bone defect repair, 9 （64.28%） achieved grade-4 healing. In contrast, little bone formation was seen in the defects even 12 weeks after the operation, and 5 （62.50%） had grade 0 healing in this group. Histologically, tissue engineering material was mostly absorbed and cartilage was found around implants in the experimental group at the 4th week; 8 weeks after operation, the engineering material was completely absorbed, and formation of woven bone was observed and typical trabecular bone structure could be seen. In control group, 8 weeks after operation, the defect was filled with fibrous tissues, and no bone-like structure was observed. Statistical analysis showed very significant difference in biomechanical indicators between the two groups （P〈0.05）. It is concluded that new oligopeptide P24 can induce excellent bone regeneration and promote bone repair.
