Background:During Enterovirus type 71(EV71)infection,the structural viral protein 1(VP1)activates endoplasmic reticulum(ER)stress associated with peripheral myelin protein 22(PMP22)accumulation and induces autophagy.H...Background:During Enterovirus type 71(EV71)infection,the structural viral protein 1(VP1)activates endoplasmic reticulum(ER)stress associated with peripheral myelin protein 22(PMP22)accumulation and induces autophagy.However,the specific mechanism behind this process remains elusive.Methods:In this research,we used the VP1-overexpressing mouse Schwann cells(SCs)models co-transfected with a PMP22 silencing or Autocrine motility factor receptor(AMFR/gp78)overexpressing vector to explore the regulation of gp78 on PMP22 and its relationship with autophagy and apoptosis.Results:The activity of gp78 could be influenced by EV71-VP1,leading to a decrease in the ubiquitination and degradation of PMP22,resulting in PMP22 accumulation in ER.In VP1-overexpressing mouse SCs,all three ER stress sensors,including pancreatic endoplasmic reticulum kinase(PERK),activating transcription factor 6(ATF6)and inositol-requiring enzyme 1(IRE1)and the related downstream signals(C/EBP-homologous protein(CHOP)and Caspase 12)were activated,as well as the ER-resident chaperone Glucose-regulated protein 78(GRP78).In addition,VP1 upregulated the autophagy marker Microtubule-associated protein 1 light chain 3 beta(LC3B),while PMP22 silencing or gp78 overexpression reversed the phenomenon.Meanwhile,PMP22 silencing or gp78 overexpression increased proliferation of EV71-VP1-transfected mouse SCs.Conclusion:Gp78 could regulate PMP22 accumulation through ubiquitination degradation and cause ER stress and autophagy in EV71-VP1-overexpressing mouse SCs.Therefore,the gp78/PMP22/ER stress axis might emerge as a promising therapeutic target for myelin and neuronal damage induced by EV71 infection.展开更多
可能最大降雨(Probable Maximum Precipitation,PMP)是评价核电厂防洪安全和排水系统设计的重要参数。在分析山东省某滨海核电厂厂址区域暴雨特性和天气成因的基础上,采用当地暴雨放大方法(水汽放大法和水汽效率放大法)和暴雨移置放大...可能最大降雨(Probable Maximum Precipitation,PMP)是评价核电厂防洪安全和排水系统设计的重要参数。在分析山东省某滨海核电厂厂址区域暴雨特性和天气成因的基础上,采用当地暴雨放大方法(水汽放大法和水汽效率放大法)和暴雨移置放大法推求出厂址24 h PMP。通过计算方案的合理性检查,对比山东省可能最大24 h点降雨量等值线图和其他核电厂工程24 h PMP成果,经综合分析确定该厂址24 h PMP为1100.0 mm,研究成果可为全国类似流域的PMP研究提供参考和借鉴。展开更多
The utilization of traffic information received from intelligent vehicle highway systems(IVHS) to plan velocity and split output power for multi-source vehicles is currently a research hotspot. However, it is an open ...The utilization of traffic information received from intelligent vehicle highway systems(IVHS) to plan velocity and split output power for multi-source vehicles is currently a research hotspot. However, it is an open issue to plan vehicle velocity and distribute output power between different supply units simultaneously due to the strongly coupling characteristic of the velocity planning and the power distribution. To address this issue, a flexible predictive power-split control strategy based on IVHS is proposed for electric vehicles(EVs) equipped with battery-supercapacitor system(BSS). Unlike hierarchical strategies to plan vehicle velocity and distribute output power separately, a monolayer model predictive control(MPC) method is employed to optimize them online at the same time. Firstly, a flexible velocity planning strategy is designed based on the signal phase and time(SPAT) information received from IVHS and then the Pontryagin’s minimum principle(PMP) is adopted to formulate the optimal control problem of the BSS. Then, the flexible velocity planning strategy and the optimal control problem of BSS are embedded into an MPC framework, which is online solved using the shooting method in a fashion of receding horizon. Simulation results verify that the proposed strategy achieves a superior performance compared with the hierarchical strategy in terms of transportation efficiency, battery capacity loss, energy consumption and computation time.展开更多
基金The study was supported by Guangdong Natural Science Foundation(Grant Numbers 2020A1515010014,2022A1515012411)Science and Technology Key Project for People’s Livelihood of Guangzhou,China(Grant Number 202206010060)+1 种基金Guangzhou Science and Technology Bureau Basic Research Project(SL2024A03J01288)Innovative Project of Children’s Research Institute,Guangzhou Women and Children’s Medical Center,China(Grant Numbers Pre-NSFC-2019-002,NKE PRE-2019-015).
文摘Background:During Enterovirus type 71(EV71)infection,the structural viral protein 1(VP1)activates endoplasmic reticulum(ER)stress associated with peripheral myelin protein 22(PMP22)accumulation and induces autophagy.However,the specific mechanism behind this process remains elusive.Methods:In this research,we used the VP1-overexpressing mouse Schwann cells(SCs)models co-transfected with a PMP22 silencing or Autocrine motility factor receptor(AMFR/gp78)overexpressing vector to explore the regulation of gp78 on PMP22 and its relationship with autophagy and apoptosis.Results:The activity of gp78 could be influenced by EV71-VP1,leading to a decrease in the ubiquitination and degradation of PMP22,resulting in PMP22 accumulation in ER.In VP1-overexpressing mouse SCs,all three ER stress sensors,including pancreatic endoplasmic reticulum kinase(PERK),activating transcription factor 6(ATF6)and inositol-requiring enzyme 1(IRE1)and the related downstream signals(C/EBP-homologous protein(CHOP)and Caspase 12)were activated,as well as the ER-resident chaperone Glucose-regulated protein 78(GRP78).In addition,VP1 upregulated the autophagy marker Microtubule-associated protein 1 light chain 3 beta(LC3B),while PMP22 silencing or gp78 overexpression reversed the phenomenon.Meanwhile,PMP22 silencing or gp78 overexpression increased proliferation of EV71-VP1-transfected mouse SCs.Conclusion:Gp78 could regulate PMP22 accumulation through ubiquitination degradation and cause ER stress and autophagy in EV71-VP1-overexpressing mouse SCs.Therefore,the gp78/PMP22/ER stress axis might emerge as a promising therapeutic target for myelin and neuronal damage induced by EV71 infection.
文摘可能最大降雨(Probable Maximum Precipitation,PMP)是评价核电厂防洪安全和排水系统设计的重要参数。在分析山东省某滨海核电厂厂址区域暴雨特性和天气成因的基础上,采用当地暴雨放大方法(水汽放大法和水汽效率放大法)和暴雨移置放大法推求出厂址24 h PMP。通过计算方案的合理性检查,对比山东省可能最大24 h点降雨量等值线图和其他核电厂工程24 h PMP成果,经综合分析确定该厂址24 h PMP为1100.0 mm,研究成果可为全国类似流域的PMP研究提供参考和借鉴。
基金supported by the National Natural Science Foundation of China (62173303)the Fundamental Research for the Zhejiang P rovincial Universities (RF-C2020003)。
文摘The utilization of traffic information received from intelligent vehicle highway systems(IVHS) to plan velocity and split output power for multi-source vehicles is currently a research hotspot. However, it is an open issue to plan vehicle velocity and distribute output power between different supply units simultaneously due to the strongly coupling characteristic of the velocity planning and the power distribution. To address this issue, a flexible predictive power-split control strategy based on IVHS is proposed for electric vehicles(EVs) equipped with battery-supercapacitor system(BSS). Unlike hierarchical strategies to plan vehicle velocity and distribute output power separately, a monolayer model predictive control(MPC) method is employed to optimize them online at the same time. Firstly, a flexible velocity planning strategy is designed based on the signal phase and time(SPAT) information received from IVHS and then the Pontryagin’s minimum principle(PMP) is adopted to formulate the optimal control problem of the BSS. Then, the flexible velocity planning strategy and the optimal control problem of BSS are embedded into an MPC framework, which is online solved using the shooting method in a fashion of receding horizon. Simulation results verify that the proposed strategy achieves a superior performance compared with the hierarchical strategy in terms of transportation efficiency, battery capacity loss, energy consumption and computation time.
