Prolylcarboxypeptidase(PRCP)belongs to the S28 family of proteases,which is also a dipeptidyl peptidase.In this study,we demonstrate the expression pattern of PRCP in Non-small cell lung cancer(NSCLC).We found that th...Prolylcarboxypeptidase(PRCP)belongs to the S28 family of proteases,which is also a dipeptidyl peptidase.In this study,we demonstrate the expression pattern of PRCP in Non-small cell lung cancer(NSCLC).We found that the repression of PRCP expression by small interfering RNA successfully inhibited cell proliferation,migration,and invasion.Further,we explored the involvement of PRCP in the regulation of epithelial-mesenchymal transition(EMT).The epithelial marker E-cadherin was significantly increased,meanwhile mesenchymal markers MUC1,vimentin,and SNAIL were markedly decreased in PRCP knockdown cells.Moreover,the downregulation of PRCP in the NSCLC cells induced the expression of apoptosis-related proteins in vitro.We performed RT-PCR in 30 pairs of clinical NSCLC tissues and adjacent non-cancerous tissues,which revealed significantly higher PRCP expression levels in cancer tissues than in adjacent non-cancerous tissues.Collectively the results from our study suggest a possible cancer promotion role of PRCP in NSCLC.展开更多
基金Scientific research projects of Nantong Health and Family Planning Commission(No.WKZD2018032).
文摘Prolylcarboxypeptidase(PRCP)belongs to the S28 family of proteases,which is also a dipeptidyl peptidase.In this study,we demonstrate the expression pattern of PRCP in Non-small cell lung cancer(NSCLC).We found that the repression of PRCP expression by small interfering RNA successfully inhibited cell proliferation,migration,and invasion.Further,we explored the involvement of PRCP in the regulation of epithelial-mesenchymal transition(EMT).The epithelial marker E-cadherin was significantly increased,meanwhile mesenchymal markers MUC1,vimentin,and SNAIL were markedly decreased in PRCP knockdown cells.Moreover,the downregulation of PRCP in the NSCLC cells induced the expression of apoptosis-related proteins in vitro.We performed RT-PCR in 30 pairs of clinical NSCLC tissues and adjacent non-cancerous tissues,which revealed significantly higher PRCP expression levels in cancer tissues than in adjacent non-cancerous tissues.Collectively the results from our study suggest a possible cancer promotion role of PRCP in NSCLC.
