Objective The activation state of microglia is known to occupy a central position in the pathophysiological process of cerebral inflammation.Autophagy is a catabolic process responsible for maintaining cellular homeos...Objective The activation state of microglia is known to occupy a central position in the pathophysiological process of cerebral inflammation.Autophagy is a catabolic process responsible for maintaining cellular homeostasis.In recent years,autophagy has been demonstrated to play an important role in neuroinflammation.Resolvin D1(RvD1)is a promising therapeutic mediator that has been shown to exert substantial anti-inflammatory and proresolving activities.However,whether RvD1-mediated resolution of inflammation in microglia is related to autophagy regulation needs further investigation.The present study aimed to explore the effect of RvD1 on microglial autophagy and its corresponding pathways.Methods Mouse microglial cells(BV-2)were cultured,treated with RvD1,and examined by Western blotting,confocal immunofluorescence microscopy,transmission electron microscopy,and flow cytometry.Results RvD1 promoted autophagy in both BV-2 cells and mouse primary microglia by favoring the maturation of autophagosomes and their fusion with lysosomes.Importantly,RvD1 had no significant effect on the activation of mammalian target of rapamycin(mTOR)signaling.Furthermore,RvD1-induced mTOR-independent autophagy was confirmed by observing reduced cytoplasmic calcium levels and suppressed calcium/calmodulin-dependent protein kinase II(CaMK II)activation.Moreover,by downregulating ATG5,the increased phagocytic activity induced by RvD1 was demonstrated to be tightly controlled by ATG5-dependent autophagy.Conclusion The present work identified a previously unreported mechanism responsible for the role of RvD1 in microglial autophagy,highlighting its therapeutic potential against neuroinflammation.展开更多
目的:探究miR-30a通过靶向调控Beclin-1、ATG5表达对食管癌细胞自噬及增殖过程的影响。方法:靶基因预测、双荧光素酶报告实验验证miR-30a对Beclin-1、ATG5的靶向调控作用。GEO、Human Protein Atlas和GEPIA在线分析TCGA数据库和GTEx项...目的:探究miR-30a通过靶向调控Beclin-1、ATG5表达对食管癌细胞自噬及增殖过程的影响。方法:靶基因预测、双荧光素酶报告实验验证miR-30a对Beclin-1、ATG5的靶向调控作用。GEO、Human Protein Atlas和GEPIA在线分析TCGA数据库和GTEx项目中食管癌组织及癌旁组织miR-30a、Beclin-1、ATG5表达、患者预后生存期。免疫组化检测45例食管癌及癌旁组织标本Beclin-1、ATG5表达,并分析Beclin-1、ATG5表达与患者临床病理参数的关系。采用脂质体LipofectamineTM3000将靶向Beclin-1、ATG5的miR-30a-mimic转染至Eca-109细胞,体外常规培养并分为3组:未转染组(control组)、转染无义RNA的阴性对照组(mimic NC组)、转染miR-30a-mimic的干扰组(miR-30a组)。MTT和克隆形成实验测定细胞增殖活力;qRT-PCR检测miR-30a、Beclin-1、ATG5 mRNA表达;划痕实验检测细胞迁移能力;Transwell小室实验检测细胞迁移和侵袭;Western blot检测Beclin-1、ATG5、LC3Ⅱ、p62蛋白表达。透射电镜观察细胞自噬泡形成情况。结果:生信分析结果显示,食管癌组织中miR-30a表达高于正常组织,Beclin-1、ATG5表达低于正常组织,且预后生存期与miR-30a表达呈负相关,与Beclin-1、ATG5表达呈正相关(P<0.05)。与control组和mimic NC组相比,miR-30a组细胞增殖、迁移和侵袭能力、p62蛋白表达明显升高,Beclin-1、ATG5、LC3Ⅱ表达、细胞自噬活力明显降低(P<0.05)。而control组与mimic NC组上述指标差异无统计学意义(P>0.05)。结论:miR-30a靶向调控Beclin-1、ATG5表达;Beclin-1、ATG5在食管癌组织和细胞中表达均降低,具有肿瘤抑制基因作用,可能通过抑制癌细胞增殖、迁移和侵袭、促进自噬发挥抑癌作用,为通过靶向调控自噬途径治疗食管癌提供了新思路。展开更多
目的探讨自噬相关基因微管相关蛋白1轻链3(microtubule-associated protein 1 light chain 3,LC3)和自噬相关基因-5(autophagy related gene-5,ATG5)在胃癌细胞发生发展不同阶段的表达情况并分析其临床意义。方法选取胃黏膜正常细胞、...目的探讨自噬相关基因微管相关蛋白1轻链3(microtubule-associated protein 1 light chain 3,LC3)和自噬相关基因-5(autophagy related gene-5,ATG5)在胃癌细胞发生发展不同阶段的表达情况并分析其临床意义。方法选取胃黏膜正常细胞、胃黏膜不典型增生细胞、胃癌细胞共58例,分别记为正常组(17例)、不典型组(19例)、胃癌组(22例),采用qRT-PCR、Western Blot、免疫组织化学法检测每组中自噬相关基因LC3和ATG5的mRNA及蛋白表达水平,应用SPSS 23.0软件分析正常组、不典型组、胃癌组3组间LC3和ATG5的mRNA及蛋白表达情况,并分析LC3和ATG5蛋白表达的相关性以及LC3和ATG5蛋白表达与胃癌患者临床病理学参数的关系。结果正常组、不典型组、胃癌组中LC3和ATG5 mRNA及蛋白表达水平逐渐升高,各组间差异均有统计学意义(P<0.05)。LC3蛋白表达情况与性别、年龄、浸润深度、分化程度及淋巴结状态均不存在相关性。ATG5蛋白表达情况与浸润深度、分化程度及淋巴结状态均有相关性。LC3和ATG5蛋白在3组中均呈正相关(P<0.05)。结论细胞自噬可促进胃癌的发生发展,其机制可能与LC3、ATG5过度表达有关。LC3、ATG5在促进胃癌的发生发展中起相互协同的作用。展开更多
目的在中国西南地区汉族人群中验证PRDM1-ATG5基因之间的rs548234 T/C单核甘酸多态性与结核病发生风险间的相关性。方法采用病例-对照研究设计,募集了234例结核病患者和208例健康对照个体。应用直接测序法对rs548234多态性进行分型,采...目的在中国西南地区汉族人群中验证PRDM1-ATG5基因之间的rs548234 T/C单核甘酸多态性与结核病发生风险间的相关性。方法采用病例-对照研究设计,募集了234例结核病患者和208例健康对照个体。应用直接测序法对rs548234多态性进行分型,采用卡方检验和Logistic回归分析,校正性别和年龄等混杂因素的影响,评价rs548234位点与结核病发生风险,以及结核病亚型的遗传关联性。结果 rs548234位点在病例和对照组中的基因型分布均符合哈-温平衡。在整个研究人群中本位点与结核病的发生风险无遗传学关联。然而我们发现原发性肺结核病患者中CC基因型频率高于健康对照人群(CC vs TT:OR=2.14,95%CI=0.91～5.10,P=0.079;CC vs CT+TT:OR=2.50,95%CI=1.09～5.73,P=0.026),提示rs548234 C等位基因可能增加原发性肺结核病的发生风险。结论在中国西南地区汉族人群中PRDM1-ATG5基因之间的rs548234 T/C多态性位点可能参与了原发性肺结核病的发生发展。展开更多
基金the National Natural Science Foundation of China(No.81902016).
文摘Objective The activation state of microglia is known to occupy a central position in the pathophysiological process of cerebral inflammation.Autophagy is a catabolic process responsible for maintaining cellular homeostasis.In recent years,autophagy has been demonstrated to play an important role in neuroinflammation.Resolvin D1(RvD1)is a promising therapeutic mediator that has been shown to exert substantial anti-inflammatory and proresolving activities.However,whether RvD1-mediated resolution of inflammation in microglia is related to autophagy regulation needs further investigation.The present study aimed to explore the effect of RvD1 on microglial autophagy and its corresponding pathways.Methods Mouse microglial cells(BV-2)were cultured,treated with RvD1,and examined by Western blotting,confocal immunofluorescence microscopy,transmission electron microscopy,and flow cytometry.Results RvD1 promoted autophagy in both BV-2 cells and mouse primary microglia by favoring the maturation of autophagosomes and their fusion with lysosomes.Importantly,RvD1 had no significant effect on the activation of mammalian target of rapamycin(mTOR)signaling.Furthermore,RvD1-induced mTOR-independent autophagy was confirmed by observing reduced cytoplasmic calcium levels and suppressed calcium/calmodulin-dependent protein kinase II(CaMK II)activation.Moreover,by downregulating ATG5,the increased phagocytic activity induced by RvD1 was demonstrated to be tightly controlled by ATG5-dependent autophagy.Conclusion The present work identified a previously unreported mechanism responsible for the role of RvD1 in microglial autophagy,highlighting its therapeutic potential against neuroinflammation.
文摘目的探讨加味丹参饮预处理是否通过调节Beclin-1和Atg5表达调控自噬抗缺血再灌注损伤大鼠心肌。方法将60只健康SD大鼠随机分为空白对照(control group,C)组、假手术(sham,S)组、缺血再灌注损伤(ischemia reperfusion injury,IRI)组、IRI+加味丹参饮(Jiawei Danshen Yin,JDY)组、IRI+JDY+自噬抑制剂(inhibitor,I)组,每组12只。通过结扎-放松大鼠左冠状动脉前降支制备心肌IRI模型。通过氯化三苯基四氮唑(TTC)染色观察心肌梗死面积率;在透射电镜下观察自噬泡;采用实时荧光定量逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)方法检测心肌Beclin-1和Atg5 m RNA表达;采用蛋白质印迹(western blot)法检测心肌Beclin-1蛋白表达变化。结果 IRI+JDY组心肌梗死面积率显著低于IRI组及IRI+JDY+I组(P<0.01)。电镜结果显示,IRI+JDY组适度调节大鼠缺血再灌注损伤心肌细胞的自噬,改善心肌细胞结构。IRI组大鼠心肌细胞中Beclin-1和Atg5 m RNA表达水平及Beclin-1蛋白表达较SG组显著升高(P<0.01);IRI+JDY组、IRI+JDY+I组大鼠心肌细胞中Beclin-1和Atg5 m RNA表达水平及Beclin-1蛋白表达较IRI组显著降低(P<0.01);IRI+JDY组大鼠心肌细胞中Beclin-1和Atg5 m RNA表达水平及Beclin-1蛋白表达较IRI+JDY+I组显著升高(P<0.01)。结论加味丹参饮通过调节缺血再灌注损伤大鼠心肌细胞自噬相关基因Beclin-1和Atg5表达适度,调控缺血再灌注心肌细胞发生适度自噬,从而发挥细胞保护作用。
文摘目的在中国西南地区汉族人群中验证PRDM1-ATG5基因之间的rs548234 T/C单核甘酸多态性与结核病发生风险间的相关性。方法采用病例-对照研究设计,募集了234例结核病患者和208例健康对照个体。应用直接测序法对rs548234多态性进行分型,采用卡方检验和Logistic回归分析,校正性别和年龄等混杂因素的影响,评价rs548234位点与结核病发生风险,以及结核病亚型的遗传关联性。结果 rs548234位点在病例和对照组中的基因型分布均符合哈-温平衡。在整个研究人群中本位点与结核病的发生风险无遗传学关联。然而我们发现原发性肺结核病患者中CC基因型频率高于健康对照人群(CC vs TT:OR=2.14,95%CI=0.91～5.10,P=0.079;CC vs CT+TT:OR=2.50,95%CI=1.09～5.73,P=0.026),提示rs548234 C等位基因可能增加原发性肺结核病的发生风险。结论在中国西南地区汉族人群中PRDM1-ATG5基因之间的rs548234 T/C多态性位点可能参与了原发性肺结核病的发生发展。
