[Objective]The research aimed to study the polymorphism of intron 1 and its effects on prolactin（PRL） gene and body size traits of Jingjiang Partridge Duck.[Method]Taking 96 Jingjiang partridge ducks as materials,th...[Objective]The research aimed to study the polymorphism of intron 1 and its effects on prolactin（PRL） gene and body size traits of Jingjiang Partridge Duck.[Method]Taking 96 Jingjiang partridge ducks as materials,the polymorphism of intron 1 of PRL gene was detected by PCR-RFLP method.And the relationship between the gene polymorphism and body size traits was analyzed.[Result]Intron 1 in PRL gene of Jingjiang Partridge Duck had sequence polymorphism.Two kinds of genotypes（AB and BB） were detected by DraⅠrestriction and genotype BB was dominant in population（91.67%）.The results of Chi-square test indicated that this site was in Hardy-Weinberg equilibrium in Jingjiang Duck（P 〉0.05）.The results of least square analysis showed that the ducks with AB genotype had larger pelvis breadth（P 〈0.01） but smaller chest measurement（P 〈0.05） than those of BB ducks.[Conclusion]It was preliminarily concluded that PRL gene could be regarded as useful candidate gene（marker） for part of body size traits of Jingjiang Partridge Duck and part of body size traits can be improved by allele selection.展开更多
Objective:To quantitatively detect the expression level of PRL-2 in primary hepatocellular carcinoma using real-time fluorescence quantitative PCR.Methods:Total RNA isolated from human HCC and liver tissue adjacent to...Objective:To quantitatively detect the expression level of PRL-2 in primary hepatocellular carcinoma using real-time fluorescence quantitative PCR.Methods:Total RNA isolated from human HCC and liver tissue adjacent to the tumor was reversely transcribed into cDNA.Real-time fluorescence quantitative PCR(Q-PCR) method was used to analyze the expres-sion level of PRL-2 gene.Results:The Q-PCR method was performed successfully to precisely detect RNA level.PRL-2 was expressed in all portal vein tumor thrombosis(PVTT) and HCC,but only in some paratumor tissue.The highest expression level of PRL-2 gene was recorded in PVTT;meanwhile expression level of PRL-2 was higher than that in paratumor liver tis-sues and in HCC(P < 0.01),and it was higher in HCC than that in paratumor liver tissues.Conclusion:The Q-PCR may be the most precise method to quantitatively detect RNA level and can be used in gene expression changes.The PRL-2 gene has higher expression in PVTT than that in HCC and in paratumor liver tissue cells,indicating that it plays an important role in the development and metastasis of the HCC.展开更多
基金Supported by Science and Technology Development of Hubei Province during the Eleventh Five-Year Plan Period (2006AA202A04)~~
文摘[Objective]The research aimed to study the polymorphism of intron 1 and its effects on prolactin（PRL） gene and body size traits of Jingjiang Partridge Duck.[Method]Taking 96 Jingjiang partridge ducks as materials,the polymorphism of intron 1 of PRL gene was detected by PCR-RFLP method.And the relationship between the gene polymorphism and body size traits was analyzed.[Result]Intron 1 in PRL gene of Jingjiang Partridge Duck had sequence polymorphism.Two kinds of genotypes（AB and BB） were detected by DraⅠrestriction and genotype BB was dominant in population（91.67%）.The results of Chi-square test indicated that this site was in Hardy-Weinberg equilibrium in Jingjiang Duck（P 〉0.05）.The results of least square analysis showed that the ducks with AB genotype had larger pelvis breadth（P 〈0.01） but smaller chest measurement（P 〈0.05） than those of BB ducks.[Conclusion]It was preliminarily concluded that PRL gene could be regarded as useful candidate gene（marker） for part of body size traits of Jingjiang Partridge Duck and part of body size traits can be improved by allele selection.
文摘Objective:To quantitatively detect the expression level of PRL-2 in primary hepatocellular carcinoma using real-time fluorescence quantitative PCR.Methods:Total RNA isolated from human HCC and liver tissue adjacent to the tumor was reversely transcribed into cDNA.Real-time fluorescence quantitative PCR(Q-PCR) method was used to analyze the expres-sion level of PRL-2 gene.Results:The Q-PCR method was performed successfully to precisely detect RNA level.PRL-2 was expressed in all portal vein tumor thrombosis(PVTT) and HCC,but only in some paratumor tissue.The highest expression level of PRL-2 gene was recorded in PVTT;meanwhile expression level of PRL-2 was higher than that in paratumor liver tis-sues and in HCC(P < 0.01),and it was higher in HCC than that in paratumor liver tissues.Conclusion:The Q-PCR may be the most precise method to quantitatively detect RNA level and can be used in gene expression changes.The PRL-2 gene has higher expression in PVTT than that in HCC and in paratumor liver tissue cells,indicating that it plays an important role in the development and metastasis of the HCC.
