Grain Yield,Biomass Accumulation,and Leaf Photosynthetic Characteristics of Rice under Combined Salinity-Drought Stress

Simultaneous stresses of salinity and drought often coincide during rice-growing seasons in saline lands,primarily due to insufficient water resources and inadequate irrigation facilities.Consequently,combined salinity-drought stress poses a major threat to rice production.In this study,two salinity levels(NS,non-salinity;HS,high salinity)along with three drought treatments(CC,control condition;DJ,drought stress imposed at jointing;DH,drought stress imposed at heading)were performed to investigate their combined influences on leaf photosynthetic characteristics,biomass accumulation,and rice yield formation.Salinity,drought,and their combination led to a shortened growth period from heading to maturity,resulting in a reduced overall growth duration.Grain yield was reduced under both salinity and drought stress,with a more substantial reduction under the combined salinity-drought stress.The combined stress imposed at heading caused greater yield losses in rice compared with the stress imposed at jointing.Additionally,the combined salinity-drought stress induced greater decreases in shoot biomass accumulation from heading to maturity,as well as in shoot biomass and nonstructural carbohydrate(NSC)content in the stem at heading and maturity.However,it increased the harvest index and NSC remobilization reserve.Salinity and drought reduced the leaf area index and SPAD value of flag leaves and weakened the leaf photosynthetic characteristics as indicated by lower photosynthetic rates,transpiration rates,and stomatal conductance.These reductions were more pronounced under the combined stress.Salinity,drought,and especially their combination,decreased the activities of ascorbate peroxidase,catalase,and superoxide dismutase,while increasing the contents of malondialdehyde,hydrogen peroxide,and superoxide radical.Our results indicated a more significant yield loss in rice when subjected to combined salinity-drought stress.The individual and combined stresses of salinity and drought diminished antioxidant enzyme activities,inhibited leaf photosynthetic functions,accelerated leaf senescence,and subsequently lowered assimilate accumulation and grain yield.

Deep learning to estimate ocean subsurface salinity structure in the Indian Ocean using satellite observations

Accurately estimating the ocean subsurface salinity structure(OSSS)is crucial for understanding ocean dynamics and predicting climate variations.We present a convolutional neural network(CNN)model to estimate the OSSS in the Indian Ocean using satellite data and Argo observations.We evaluated the performance of the CNN model in terms of its vertical and spatial distribution,as well as seasonal variation of OSSS estimation.Results demonstrate that the CNN model accurately estimates the most significant salinity features in the Indian Ocean using sea surface data with no significant differences from Argo-derived OSSS.However,the estimation accuracy of the CNN model varies with depth,with the most challenging depth being approximately 70 m,corresponding to the halocline layer.Validations of the CNN model’s accuracy in estimating OSSS in the Indian Ocean are also conducted by comparing Argo observations and CNN model estimations along two selected sections and four selected boxes.The results show that the CNN model effectively captures the seasonal variability of salinity,demonstrating its high performance in salinity estimation using sea surface data.Our analysis reveals that sea surface salinity has the strongest correlation with OSSS in shallow layers,while sea surface height anomaly plays a more significant role in deeper layers.These preliminary results provide valuable insights into the feasibility of estimating OSSS using satellite observations and have implications for studying upper ocean dynamics using machine learning techniques.

Common Organic Amendment (Rice Straw) Can Reduce Salinity Effects on Bean (Phaseolus vulgaris) Growth with or without Photoperiod Manipulation

Soil salinity is a major limiting factor for crop production in coastal areas of Bangladesh. Cheap and sustainable management of soil salinity is hence most sought out topics in agricultural research. Conceptualizing that idea in mind, a pot experiment was conducted in the Department of Soil, Water & Environment, University of Dhaka in order to analyze if common organic amendments (rice straw, saw dust) coupled with reduce photoperiod can mitigate salinity effect on the growth of bean (Phaseolus vulgaris). The experiment was set up following completely randomized design (CRD) with nine treatments and three replications containing Tc (Control), T1 (Ambient photoperiod + 110 mM Salinity treatment + Rice straw), T2 (Reduced photoperiod + 110 mM Salinity treatment + Rice straw), T3 (Ambient photoperiod + 220 mM Salinity treatment + Rice straw), T4 (Reduced photoperiod + 220 mM Salinity treatment + Rice straw), T5 (Ambient photoperiod + 110 mM Salinity treatment + Saw dust), T6 (Reduced photoperiod + 110 mM Salinity treatment + Saw dust), T7 (Ambient photoperiod + 220 mM Salinity treatment + Saw dust) and T8 (Reduced photoperiod + 220 mM Salinity treatment + Saw dust). Organic amendments were used separately at the rate of 12 ton/ha. The highest plant height (98.67 cm), root length (12.5 cm), pod number (10.33), leaf area (13.99 cm2), fresh weight (680 kg/ha), dry weight (316.67 kg/ha) were recorded with the treatment T1 while the second-best treatment was treatment T2 (with highest harvest index 0.040) and these results were statistically significant (p < 0.001). In post-harvest soil, pH, EC, OC, OM;available N, P, K, S;total Ca, Mg, Zn, Mn were increased significantly in treatment T1. The overall results illustrated that the best growth and yield performances were achieved in the treatment T1 and T2.

Salinity Stress Deteriorates Grain Yield and Increases 2-Acetyl-1-Pyrroline Content in Rice

Salinity stress greatly impacts rice grain yield and quality, as well as the 2-acetyl-1-pyrroline(2-AP) content in grains. The present study was conducted with Nanjing 9108(NJ9108, conventional japonica rice) and Wenliangyou 669(WLY669, indica hybrid rice) in the fields with non-salinity(NS), low salinity(LS), and high salinity(HS) stresses in 2021 and 2022.

Salinity fronts shape spatial patterns in zooplankton distribution in Hangzhou Bay

Ocean fronts play important roles in nutrient transport and in the shaping ecological patterns.Frontal zones in small bays are typically small in scale,have a complex structure,and they are spatially and temporally variable,but there are limited data on how biological communities respond to this variation.Hangzhou Bay,a mediumsized estuary in China,is an ideal place in which to study the response of plankton to small-scale ocean fronts,because three water masses(Qiantang River Diluted Water,Changjiang River Diluted Water,and the East China Sea current) converge here and form dynamic salinity fronts throughout the year.We investigate zooplankton communities,and temperature,salinity and chlorophyll a(Chl a) in Hangzhou Bay in June(wet perio d) and December(dry period) of 2022 and examine the dominant environmental factors that affect zooplankton community spatial variability.We then match the spatial distributions of zooplankton communities with those of salinity fronts.S alinity is the most important explanatory variable to affect zooplankton community spatial variability during both wet and dry periods,in that it contributes>60% of the variability in community structure.Furthermore,the spatial distributions of zooplankton match well with salinity fronts.During December,with weaker Qiantang River Diluted Water and a stronger secondary Changjiang River Plume,zooplankton communities occur in moderate salinity(MS,salinity range 15.6±2.2) and high salinity(HS,22.4±1.7) regions,and their ecological boundaries closely match the Qiantang River Diluted Water front.In June,different zooplankton communities occur in low salinity(LS,3.9±1.0),MS(11.7±3.6) and HS(21.3±1.9) regions.Although the LS region occurs abnormally in the central bay rather than its apex because of the anomalous influence of rising and falling tides during the sampling perio d,the ecological boundaries still match salinity interfaces.Low-salinity or brackish-water zooplankter taxa are relatively more abundant in LS or MS regions,and the biomass and abundance of zooplankton is higher in the MS region.

Synthesizing high-resolution satellite salinity data based on multi-fractal fusion

The spaceborne platform has unprecedently provided the global eddy-permitting(typically about 0.25°)products of sea surface salinity(SSS),however the existing SSS products can hardly resolve mesoscale motions due to the heavy noises therein and the over-smoothing in denoising processes.By means of the multi-fractal fusion(MFF),the high-resolution SSS product is synthesized with the template of sea surface temperature(SST).Two low-resolution SSS products and four SST products are considered as the source data and the templates respectively to determine the best combination.The fused products are validated by the in situ observations and intercompared via SSS maps,Singularity Exponent maps and wavenumber spectra.The results demonstrate that the MFF can perform a good work in mitigating the noises and improving the resolution.The combination of the climate change initiative SSS and the remote sensing system SST can produce the 0.1°denoised product whose global mean standard derivation of salinity against Argo is 0.21 and the feature resolution can reach 30−40 km.

Spatio-temporal variation of water salinity in mangroves revealed by continuous monitoring and its relationship to floristic diversity

Salinity is among the most critical factors limiting the growth and species distribution of coastal plants.Water salinity in estuarine ecosystems varies temporally and spatially,but the variation patterns across different time scales and salinity fluctuation have rarely been quantified.The effects of salinity on floristic diversity in mangroves are not fully understood due to the temporal and spatial heterogeneity of salinity.In this study,we monitored water salinity at an interval of 10-min over one year in three mangrove catchment areas representing the outer part,middle part,and inner part respectively of Dongzhai Bay,Hainan,China.The number of mangrove community types and dominant mangrove species of the three catchment areas were also investigated.We found that the diurnal variation and dry-season intra-month variation in water salinity were driven by tidal cycles.The seasonal variation in water salinity was mainly driven by rainfall with higher salinity occurring in the dry season and lower salinity occurring in the wet season.Spatially,water salinity was highest at the outer part,intermediate at the middle part,and lowest at the inner part of the bay.The intra-month and annual fluctuations of water salinity were highest at the middle part and lowest at the outer part of the bay.The number of mangrove community types and dominant species were lowest at the outer part,intermediate at the middle part,and highest at the inner part of the bay.These results suggest that the temporal variation of water salinity in mangroves is driven by different factors at different time scales and therefore it is necessary to measure water salinity at different time scales to get a complete picture of the saline environment that mangroves experience.Spatially,lower salinity levels benefit mangrove species richness within a bay landscape,however,further research is needed to distinguish the effects of salinity fluctuation and salinity level in affecting mangrove species richness.

Experimental investigation into the salinity effect on the physicomechanical properties of carbonate saline soil

For engineering structures with saline soil as a filling material,such as channel slope,road subgrade,etc.,the rich soluble salt in the soil is an important potential factor affecting their safety performance.This study examines the Atterberg limits,shear strength,and compressibility of carbonate saline soil samples with different NaHCO3 contents in Northeast China.The mechanism underlying the influence of salt content on soil macroscopic properties was investigated based on a volumetric flask test,a mercury intrusion porosimetry(MIP)test,and a scanning electron microscopic(SEM)test.The results demonstrated that when NaHCO3 contents were lower than the threshold value of 1.5%,the bound water film adsorbed on the surface of clay particles thickened continuously,and correspondingly,the Atterberg limits and plasticity index increased rapidly as the increase of sodium ion content.Meanwhile,the bonding force between particles was weakened,the dispersion of large aggregates was enhanced,and the soil structure became looser.Macroscopically,the compressibility increased and the shear strength(mainly cohesion)decreased by 28.64%.However,when the NaHCO3 content exceeded the threshold value of 1.5%,the salt gradually approached solubility and filled the pores between particles in the form of crystals,resulting in a decrease in soil porosity.The cementation effect generated by salt crystals increased the bonding force between soil particles,leading to a decrease in plasticity index and an improvement in soil mechanical properties.Moreover,this work provides valuable suggestions and theoretical guidance for the scientific utilization of carbonate saline soil in backfill engineering projects.

Salinity Acclimation Induces Reduced Energy Metabolism,Osmotic Pressure Regulation and Transcriptional Reprogramming in Hypotrichida Ciliate Gastrostyla setifera

Coastal and estuarine protists are frequently exposed to salinity undulation.While the tolerance and stress responses of microalgae to salinity have been extensively studied,there have been scarce studies on the physiological response of heterotrophic protists to salinity stressing.In this study,we investigated the physiological response of the heterotrophic ciliate Gastrostyla setifera to a salinity of 3,via a transcriptomic approach.The first transcriptome of genus Gastrostyla was obtained utilizing a group of manually isolated ciliate individuals(cells)and RNA-seq technique.The completeness of the transcriptome was verified.Differentially expressed gene(DEG)analysis was performed among the transcriptomes of G.setifera acclimated in saline water(salinity 3)and those cultured in fresh water.The results demonstrated a significant alternation in gene transcription,in which the ciliate exhibits a transcripttomic acclimation in responding salinity stressing.The up-regulated DEGs were enriched in the pathways of cytoskeleton proteins,membrane trafficking,protein kinases and protein phosphatases.These may represent enhanced functions of ion transport,stress response and cell protections.Pathways involved in energy metabolism and biosynthesis were markedly down-regulated,reflecting decreased cell activity.Particularly,we detected significantly down-regulated genes involved in several pathways of amino acid catabolism,which may lead to accumulation of amino acids in the ciliate cell.Amino acid could act as compatible solutes in the cytoplasm to maintain the osmotic balance in saline water.Overall,this work is an initial exploration to the molecular basis of the heterotrophic protist responding to salinity stressing.The result sheds light on the mechanisms of enhancement of cell protection,reduction of cell activity,and osmotic pressure regulation in ciliates acclimated to salinity.

RNA interference reveals chloride channel 7 gene helps short-term hypersalinity stress resistance in Hong Kong oyster Crassostrea hongkongensis

The chloride channel 7 gene(CLC 7)of the Hong Kong oyster Crassostrea hongkongensis was cloned and named ChCLC 7.The cDNA was 2572 bp in length,with a 5′non-coding region containing 25 bp,a 3′non-coding region containing 327 bp,and an open reading frame of 2298 bp.ChCLC 7 has 96.8%and 92.1%homology with CLC 7 of Crassostrea gigas and Crassostrea virginica,respectively,and it was clustered with CLC 7 of C.gigas and C.virginica.QRT-PCR showed that ChCLC 7 was expressed in all eight tissues,with the highest in adductor muscle and second in gill.The ChCLC 7 expression pattern in gill was altered significantly under high salinity stress with an overall upward and then downward trend.After RNA interference,the expression of ChCLC 7 and survival rate of oyster under high salinity stress was reduced significantly,and so did the concentration of hemolymph chloride ion in 48-96 h after RNA interference.We believed that ChCLC 7 could play an important role in osmoregulation of C.hongkongensis by regulating Cl^(-)transport.This study provided data for the analysis of molecular mechanism against oyster salinity stress.

A salt-induced tackifying polymer for enhancing oil recovery in highsalinity reservoirs:Synthesis,evaluation,and mechanism

Polymerflooding is an effective method widely applied for enhancing oil recovery(EOR)by reducing the mobility ratio between theinjected water and crude oil.However,traditional polymers encounter challenges in high salinity reservoirs due to their salt sensitivity.Toovercome this challenge,we synthesized a zwitterion polymer(PAMNS)with salt-induced tackifying property through copolymerization ofacrylamide and a zwitterion monomer,methylacrylamide propyl-N,N-dimethylbutylsulfonate(NS).NS monomer is obtained from thereaction between 1,4-butanesultone and dimethylamino propyl methylacrylamide.In this study,the rheological properties,salt responsiveness,and EOR efficiency of PAMNS were evaluated.Results demonstrate that PAMNS exhibits desirable salt-induced tackifyingcharacteristics,with viscosity increasing up to 2.4 times as the NaCl concentration reaches a salinity of 30×10^(4)mg L^(-1).Furthermore,highvalence ions possess a much stronger effect on enhancing viscosity,manifested as Mg^(2+)>Ca^(2+)>Na^(+).Molecular dynamics simulations(MD)andfluid dynamics experiment results demonstrate that PAMNS molecules exhibit a more stretched state and enhanced intermolecularassociations in high-salinity environments.It is because of the salt-induced tackifying,PAMNS demonstrates superior performance inpolymerflooding experiments under salinity ranges from 5×10^(4)mg L^(-1)to 20×10^(4)mg L^(-1),leading to 10.38–19.83%higher EOR thantraditional polymers.

Temperature and Salinity Dual-parameter Sensing Based on Forward Brillouin Scattering in 1060-XP SMF

A novel temperature and salinity discriminative sensing method based on forward Brillouin scattering(FBS)in 1060-XP single-mode fiber(SMF)is proposed.The measured frequency shifts corresponding to different radial acoustic modes in 1060-XP SMF show different sensitivities to temperature and salinity.Based on the new phenomenon that different radial acoustic modes have different frequency shift-temperature and frequency shift-salinity coefficients,we propose a novel method for simultaneously measuring temperature and salinity by measuring the frequency shift changes of two FBS scattering peaks.In a proof-of-concept experiment,the temperature and salinity measurement errors are 0.12℃and 0.29%,respectively.The proposed method for simultaneously measuring temperature and salinity has the potential applications such as ocean surveying,food manufacturing and pharmaceutical engineering.

Plasticity of photorespiratory carbon concentration mechanism in Sedobassia sedoides(Pall.)Freitag&G.Kadereit under elevated CO_(2)concentration and salinity

Rising atmospheric CO_(2)(carbon dioxide)concentrations and salinization are manifestations of climate change that affect plant growth and productivity.Species with an intermediate C_(3)-C_(4)type of photosynthesis live in a wide range of precipitation,temperature,and soil quality,but are more often found in warm and dry habitats.One of the intermediate C_(3)-C_(4)photosynthetic type is C_(2)photosynthesis with a carbon concentration mechanism(CCM)that reassimilates CO_(2)released via photorespiration.However,the ecological significance under which C_(2)photosynthesis has advantages over C_(3)and C_(4)plants remains largely unexplored.Salt tolerance and functioning of CCM were studied in plants from two populations(P1 and P2)of Sedobassia sedoides(Pall.)Freitag&G.Kadereit Asch.species with C_(2)photosynthesis exposed to 4 d and 10 d salinity(200 mM NaCl)at ambient(785.7 mg/m^(3),aCO_(2)and elevated(1571.4 mg/m^(3),eCO_(2))CO_(2).On the fourth day of salinity,an increase in Na+content,activity catalase,and superoxide dismutase was observed in both populations.P2 plants showed an increase in proline content and a decrease in photosynthetic enzyme content:rubisco,phosphoenolpyruvate carboxylase(PEPC),and glycine decarboxylase(GDC),which indicated a weakening of C_(2)and C_(4)characteristics under salinity.Treatment under 10 d salinity led to an increased Na^(+)content and activity of cyclic electron flow around photosystem I(PSI CEF),a decreased content of K^(+)and GDC in both populations.P1 plants showed greater salt tolerance,which was assessed by the degree of reduction in photosynthetic enzyme content,PSI CEF activity,and changes in relative growth rate(RGR).Differences between populations were evident under the combination of eCO_(2)and salinity.Under long-term salinity and eCO_(2),more salt-tolerant P1 plants had a higher dry biomass(DW),which was positively correlated with PSI CEF activity.In less salt-tolerant P2 plants,DW correlated with transpiration and dark respiration.Thus,S.sedoides showed a high degree of photosynthetic plasticity under the influence of salinity and eCO_(2)through strengthening(P1 plants)and weakening C_(4)characteristics(P2 plants).

Salinity effect-induced ENSO amplitude modulation in association with the interdecadal Pacific Oscillation

A 110-year ensemble simulation of an ocean general circulation model(OGCM)was analyzed to identify the modulation of salinity interdecadal variability on El Niño-Southern Oscillation(ENSO)amplitude in the tropical Pacific during 1901-2010.The simulating results show that sea surface salinity(SSS)variation in the region exhibits notable and coherent interdecadal variability signal,which is closely associated with the Interdecadal Pacific Oscillation(IPO).As salinity increases or reduces,the SSS modulations on ENSO amplitude during its warm/cold events vary asymmetrically with positive/negative IPO phases.Physically,salinity interdecadal variability can enhance or reduce ENSO-related conditions in upper-ocean stratification,contributing noticeably to ENSO variability.Salinity anomalies associated with the mixed layer depth and barrier layer thickness can modulate ENSO amplitude during positive and negative IPO phases,resulting in the asymmetry of sea surface temperature(SST)anomaly in the tropical Pacific.During positive IPO phases,SSS interdecadal variability contributes positively to El Niño amplitude but negatively to La Niña amplitude by enhancing or reducing SSS interannual variability,and vice versa during negative IPO phases.Quantitatively,the results indicate that the modulation of the ENSO amplitude by the SSS interdecadal variability is 15%-28%during negative IPO phases and 30%-20%during positive IPO phases,respectively.Evidently,the SSS interdecadal variability associated with IPO and its modulation on ENSO amplitude in the tropical Pacific are among factors essentially contributing ENSO diversity.

Variability in Quantity and Salinity of Produced Water from an Oil Production in South Kuwait

Produced water (PW) is the largest waste stream in the oil and gas industry. Water remains trapped for millions of years in the reservoir with oil and gas. When a hydrocarbon reservoir is infiltrated by a production well, the produced fluids commonly contain water. The understanding of this water’s constituents and volumes is vital for the sustainable continuity of production operations, as PW has a number of negative impacts on the infrastructure integrity of the operation. On the other hand, PW can be an alternative source of irrigation water as well as of industrial salt. Interestingly, both the quantity as well as the quality of PW do not remain constant but can vary, both progressively and erratically, even over short periods of time. This paper discusses such a situation of variable PW in an oil and gas operation in the State of Kuwait.

Effects of Salinity and Dietary Chinese Herbal Medicine on Survival and Growth of Juvenile Fenneropenaeus chinensis

[Objective] The aim of this study was to provide theoretical basis for the large-scale breeding of Fenneropenaeus chinensis as well as the promotion of Chinese herbal medicine.[Method] The experiment was conducted to determine the effects of salinity（12‰,20‰ and 28‰）and dietary Chinese herbal medicine（CHM：0%,1%,2% and 4%）on survival and growth of juvenile F.chinensis with the average initial wet body weight of 0.027±0.005 g.The experiment lasted for 5 weeks.[Result] The survival rate,specific growth rate,food consumption and apparent digestibility coefficient was increased with CHM level increasing;when attained the critical value,the survival rate,specific growth rate,food consumption and apparent digestibility coefficient decreased with CHM level increasing.Food conversion ratio decreased with CHM level increasing,and then it increased after attained the critical value.At each CHM level,the survival rate,specific growth rate,food consumption and apparent digestibility coefficient were highest at salinity of 20‰,while food conversion ratio was lowest at salinity 20‰.[Conclusion] Salinity and CHM had a significant effect on growth of F.chinensis,which should be paid more attention in the breeding.

Effects of Adaptation to Elevated Salinity on Some Enzymes' Salt_tolerance in Vitro and Physiological Changes of Eelgrass

The eelgrass ( Zostera marina L.) was treated with artificial seawater (ASW) of different salinities ( 100%, 150% and 200% seawater) for 5 d. The activities of two enzymes extracted from the plant leaves were determined under a salinity grade in vitro So were the photosynthesis rates of the plants from the three treatments in the media with different salinities 100%, 150%, 200%, 300% ASW) and Some physiological data. The data showed that under increased salinities (concentrated seawater), Na+, Cl-, MDA (malon dialdehyde) and glucose contents and the osmotic potentials ( absolute value) in the leaves increased with the salinity elevation in the medium (ASW), but both K+ and free amino acid (mainly proline) contents decreased. Malate dehydrogenase (MDH) from the plant leaves under a salinity grade showed its activities (A) as follows: A(100%) (ASW) > A(150%) (ASW) > A(200%) (ASW). Phosphoenolpyruvate carboxylase (PEPC) extracted from the 100% ASW- and 200% ASW-treated plants showed similar activities (both insensitive to salinities) under the salinity grade in vitro, but the activities of PEPC from plants treated with 150% ASW were dependent oil salinity. Whether the plant is stressed at 150% ASW and can stand higher salinity than seawater needs to be studied further. Meantime, die data do not agree with the opinion that the adaptation of the eelgrass to seawater salinity is partly fulfilled by its insensitiveness to salinities in Some metabolic enzymes. It can be inferred that the lack of transpiration may be an important aspect of tire plant's tolerance to seawater salinity.

Effects of salinity on growth and feeding of juvenile Starry flounder (Platichthys stellatus)

The survival rate, feeding, growth of juvenile Starry flounder were studied at different salinities. The results showed that when the fish was directly translated from salinity of 32 to 0 and more than 0, the survival rate was 100% in 96 hours. If the fish was acclimatized, it was still 100% in 42 days. The fish at the salinity of 0 - 16 grew faster than others; feeding rate varied as salinity changed, and the highest one was at salinity of 32 and the lowest one was at the salinity of 16. As to feed conversion efficiency, the highest one was at the salinity of 16, and the lowest one was at the salinity of 24. There were no significant differences among the treatments （P＆gt;0.05）. It was found that low salinity benefited for recovering albinism.

Effects of salinity fluctuation and dietary protein/ carbohydrate ratio on the survival and growth of Juvenile Litopenaeus vannamei

A factorial study was conducted to determine the effects of salinity （constant （SO） and fluctuating （S10）） and isoenergetic diet of different protein/carbohydrate ratios （P/C： 4.1, 1.9, 1.0 and 0.6） on the survival and growth of juvenile Litopenaeus vannamei. The experiment lasted for 35 days. The results showed that I） At S0 and S10, specific growth rate and apparent digestibility coefficient exhibited decreasing trend, while food conversion ratio exhibited increasing trend, with decreasing P/C ratio. At SO, food consumption and specific growth rate of the shrimp fed 4.1 P/C were significantly higher than those fed 1.0 and 0.6 P/C, whereas at S10, no significant differences were detected among different treatments; II） At 4.1 and 1.9 P/C, food consumption and specific growth rate were higher in the shrimp maintained at SO as compared with those at S10, while at 1.0 and 0.6 P/C, an inverse trend was observed. Among four P/C ratios, food conversion ratio of the shrimp at SO was the lowest.

Effects of Salinity Fluctuation and Dietary Traditional Chinese Herbal Medicines on Survival,Growth and Immunity of Exopalaemon carinicauda

[Objective]The aim of this study was to provide a theoretical basis for the large-scale breeding of Exopalaemon carinicauda. [Method]Effects of salinity fluctuation （S0,S3,S6 and S9 represented salinity fluctuations of 0,3,6 and 9 respectivley） and dietary traditional Chinese herbal medicines （0,1%,2% and 4% respectively） on survival,growth and immunity of E.carinicauda were studied,and its experiment lasted for 60 d. [Result]The results showed that specific growth rate,feed conversion efficiency and superoxide dismutase activity of E.carinicauda in the treatment group with 1% dietary traditional Chinese herbal medicine and S6 salinity fluctuation were higher than those in other treatments,which was significantly higher than the treatment groups with S0,S3 and S9 salinity fluctuation at the same adding level of 1% dietary traditional Chinese herbal medicine. The regression analysis indicated that at S0,S3 and S6 salinity fluctuation,the optimal adding level of dietary traditional Chinese herbal medicines in feeds decreased with the increase of salinity fluctuation,accounting for 2.74%,1.77% and 0.51% respectively. At four adding levels of dietary traditional Chinese herbal medicines,the optimal salinity fluctuation of E.carinicauda also decreased with the increase of dietary traditional Chinese herbal medicines in feeds,accounting for 5.26,4.95,3.54 and 1.97 respectively. [Conclusion]Salinity,dietary traditional Chinese herbal medicines and their interactions have a significant effect on survival,growth and immunity of E.carinicauda,which calls for attentions in the breeding.