BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs ...BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs pulse-width-modulated (PWM) voltage that applies a higher average voltage to a larger protein species. BlotMan can be controlled not only by its custom-made interface but also by a smart phone via Bluetooth technology. In this study, we examined effects of PWM signals (50%, 60%, and 80% duty cycle) on transfer efficiency and signal intensity in comparison to a constant voltage signal (100% duty cycle). The result revealed that in response to the same average voltage of 150 V, a lower duty cycle with a higher maximum voltage increased transfer efficiency as well as sharpness of transferred proteins. We validated BlotMan’s capability using a chondrosarcoma cell line (SW1353 cells) and a breast cancer cell line (MDA-MB231 cells) in response to antitumor chemical agents. BlotMan successfully generated 5 membranes from a single gel and detected 5 protein species such as c-Src, eukaryotic translation initiation factor 2 alpha (eIF2), phosphorylated eIF2, lamin B, and actin. Collectively, we demonstrated herein that BlotMan reduces an amount of protein samples by generating multiple membranes from a single gel and improving signal intensity with PWM voltage signals.展开更多
Since the poor performance of orthogonal binary Pulse Position Modulation (PPM) compared with binary Pulse Amplitude Modulation (PAM), this paper presents a new modulation scheme named Pulse Width Modulation (PWM) for...Since the poor performance of orthogonal binary Pulse Position Modulation (PPM) compared with binary Pulse Amplitude Modulation (PAM), this paper presents a new modulation scheme named Pulse Width Modulation (PWM) for Impulse Radio Ultra-WideBand (IR-UWB) communication systems. This modulation scheme uses pulses with equal amplitude and different widths to carry different information. The receiver employs differences between similarity coefficients among these pulses to distinguish different information. Both theoretical analysis and simulation results verify that this novel scheme has a Signal to Noise Ratio (SNR) gain of about 1.75 dB compared with or- thogonal binary PPM, and has an SNR loss of about 1.4 dB compared with binary PAM. Although both the theoretical analysis and simulations are based on time-hopping multiple access, this modulation scheme can also be applied to other accessing techniques of UWB communication systems.展开更多
A test bench of ABS/ASR integrated hydraulic system is developed by using pulse-width modulation （PWM） technology. The effective duty ratio range of ABS outlet valve has been tested in PWM control. With 50 Hz carrie...A test bench of ABS/ASR integrated hydraulic system is developed by using pulse-width modulation （PWM） technology. The effective duty ratio range of ABS outlet valve has been tested in PWM control. With 50 Hz carrier wave frequency, the tests are performed to determine the correspondence between duty ratio and the wheel cylinder pressure variation. The duty ratio range of ABS outlet valve in PWM control is determined and an experimental model of pressure reduction velocity （PRV） of wheel cylinder using PWM control is established. By comparison and test of the experimental model and realization of controlling the duty ratio of ABS outlet valves, the fine regulation of wheel cylinder PRV is realized in the working of ABS/ASR braking regulation, which is important and valuable to the improvement of the ABS/ASR controlling effect.展开更多
Instead of avoiding occurrence of chaos in motor drives, chaos is positively utilized in this paper. A new chaotic pulse width modulation （PWM） scheme is proposed and implemented for AC motors, which functions to si...Instead of avoiding occurrence of chaos in motor drives, chaos is positively utilized in this paper. A new chaotic pulse width modulation （PWM） scheme is proposed and implemented for AC motors, which functions to significantly suppress harmonic peaks and hence acoustic noise. The key is to employ the Chua＇s circuit for generating a desired chaotic sequence. By using a practical induction motor, computer simulation and experimental results verify that the chaotic PWM has advantages of harmonic peak suppression and simple hardware implementation over the conventional PWM and random PWM.展开更多
The present paper deals with the development of a modular, flexible and structured block to block approach for the study of regulators by implementing the different blocks on a DSP （digital signal processor）. The pr...The present paper deals with the development of a modular, flexible and structured block to block approach for the study of regulators by implementing the different blocks on a DSP （digital signal processor）. The proposed low-cost approach has been applied and validated by the implementation of an industrial regulator in a real time hardware-in-the-loop simulation of a mixed islanded power network including precise models of the hydraulic system. The studied network is constituted of three different types of electrical power generation systems and a consumer.展开更多
文摘BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs pulse-width-modulated (PWM) voltage that applies a higher average voltage to a larger protein species. BlotMan can be controlled not only by its custom-made interface but also by a smart phone via Bluetooth technology. In this study, we examined effects of PWM signals (50%, 60%, and 80% duty cycle) on transfer efficiency and signal intensity in comparison to a constant voltage signal (100% duty cycle). The result revealed that in response to the same average voltage of 150 V, a lower duty cycle with a higher maximum voltage increased transfer efficiency as well as sharpness of transferred proteins. We validated BlotMan’s capability using a chondrosarcoma cell line (SW1353 cells) and a breast cancer cell line (MDA-MB231 cells) in response to antitumor chemical agents. BlotMan successfully generated 5 membranes from a single gel and detected 5 protein species such as c-Src, eukaryotic translation initiation factor 2 alpha (eIF2), phosphorylated eIF2, lamin B, and actin. Collectively, we demonstrated herein that BlotMan reduces an amount of protein samples by generating multiple membranes from a single gel and improving signal intensity with PWM voltage signals.
文摘Since the poor performance of orthogonal binary Pulse Position Modulation (PPM) compared with binary Pulse Amplitude Modulation (PAM), this paper presents a new modulation scheme named Pulse Width Modulation (PWM) for Impulse Radio Ultra-WideBand (IR-UWB) communication systems. This modulation scheme uses pulses with equal amplitude and different widths to carry different information. The receiver employs differences between similarity coefficients among these pulses to distinguish different information. Both theoretical analysis and simulation results verify that this novel scheme has a Signal to Noise Ratio (SNR) gain of about 1.75 dB compared with or- thogonal binary PPM, and has an SNR loss of about 1.4 dB compared with binary PAM. Although both the theoretical analysis and simulations are based on time-hopping multiple access, this modulation scheme can also be applied to other accessing techniques of UWB communication systems.
基金the National Natural Science Foundation of China (50122155)
文摘A test bench of ABS/ASR integrated hydraulic system is developed by using pulse-width modulation （PWM） technology. The effective duty ratio range of ABS outlet valve has been tested in PWM control. With 50 Hz carrier wave frequency, the tests are performed to determine the correspondence between duty ratio and the wheel cylinder pressure variation. The duty ratio range of ABS outlet valve in PWM control is determined and an experimental model of pressure reduction velocity （PRV） of wheel cylinder using PWM control is established. By comparison and test of the experimental model and realization of controlling the duty ratio of ABS outlet valves, the fine regulation of wheel cylinder PRV is realized in the working of ABS/ASR braking regulation, which is important and valuable to the improvement of the ABS/ASR controlling effect.
基金Project supported by the Shanghai Leading Academic Discipline Project (Grant No.T0103)
文摘Instead of avoiding occurrence of chaos in motor drives, chaos is positively utilized in this paper. A new chaotic pulse width modulation （PWM） scheme is proposed and implemented for AC motors, which functions to significantly suppress harmonic peaks and hence acoustic noise. The key is to employ the Chua＇s circuit for generating a desired chaotic sequence. By using a practical induction motor, computer simulation and experimental results verify that the chaotic PWM has advantages of harmonic peak suppression and simple hardware implementation over the conventional PWM and random PWM.
文摘The present paper deals with the development of a modular, flexible and structured block to block approach for the study of regulators by implementing the different blocks on a DSP （digital signal processor）. The proposed low-cost approach has been applied and validated by the implementation of an industrial regulator in a real time hardware-in-the-loop simulation of a mixed islanded power network including precise models of the hydraulic system. The studied network is constituted of three different types of electrical power generation systems and a consumer.
