Research Progress of Paederia scandens(Lour.)Merr in Modern Pharmacy

Paederia scandens(Lour.)Merr.is a traditional Chinese medicine,which mainly has the functions of analgesia,detoxification,anti-inflammatory and anticonvulsant.Modern studies show that the main chemical constituents of this plant are iridoids and volatile oil,which have biological activities such as analgesia,anti-inflammatory,hepatoprotection,renoprotection and antibacterial.This review highlights the chemical constituents and biological activities of P.scandens based on studies published in last decades and provides the references for the further development and utilization of this medicinal plant.

A new iridoid glycoside from Paederia scandens

A new glycoside named 6β-O-β-D-glucosylpaederosidic acid(1) was isolated from Paederia scandens and its structure was elucidated on the basis of spectroscopic and chemical evidence,together with four known iridoids,paederoside(2),paederosidic acid(3),paederosidic acid methyl ester(4),and deacetyl asperulosidic acid methyl ester(5).Compound 5 was isolated from this plant for the first time.

Effects of Different Drying Methods on Content of Paederosidic Acid in Paederia scandens

[Objectives]To establish a method to simultaneously determine the content of paederosidic acid in Paederia scandens dried by four different methods.[Methods]Reversed-phase high performance liquid chromatography(RP-HPLC)was adopted.The chromatographic column:Thermo SCIENTIFIC Hypersil GOLD Dim.(mm);mobile phase:acetonitrile-0.1%phosphoric acid aqueous solution;gradient elution;column temperature:30℃;flow rate:1 mL/min;detection wavelength:236 nm;injection volume:10μL.[Results]The linear range of the detection injection volume of paederosidic acid was 0.64-9.60μg(R=0.9992);the limit of quantity(LOQ)was 5.10 ng,and the limit of detection(LOD)was 1.36 ng;the RSD of the precision,stability and reproducibility test was all less than 3%;the sample recovery rate was 98.87%,RSD<3.00,n=6.The results show that the content of paederosidic acid in the shade-dried P.scandens is the highest,and the order of the content is P.scandens(dry in shade)>P.scandens(dried at 50℃)>P.scandens(dried at 60℃)>P.scandens(dried at 70℃).[Conclusions]This method is sensitive,reliable and reproducible,and can be used to simultaneously determine the content of paederosidic acid in P.scandens dried by four different methods.

UPLC/Q-TOF-MS analysis of iridoid glycosides and metabolites in rat plasma after oral administration of Paederia scandens extracts

A rapid and validated UPLC-MS method was developed for investigating the absorbed components of Paederia scandens(Lour.) Merrill(P.scandens) in rat plasma.The bioactive constituents in plasma samples from rats administrated orally with P.scandens extract were analyzed by Ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS).Four prototype compounds were identified in rat serum as potential bioactive components of P.scandens by comparing their retention times and mass spectrometry data or by mass spectrometry analysis and retrieving the reference literatures.Glucuronidation after deglycosylation was the major metabolic pathway for the iridoid glycosides in P.scandens.These results showed that the methods had high sensitivity and resolution and were suitable for identifying the bioactive constituents in plasma after oral administration of P.scandens.providing helpful chemical information for further pharmacological and mechanistic researched on the P.scandens.

鸡屎藤化学成分的研究

目的：研究鸡屎藤Paederia scandense的化学成分。方法：采用硅胶柱、Sephadex LH-20柱进行分离和纯化，通过理化常数和波谱分析进行化合物的结构鉴定。结果：分离到20个化合物，鉴定为茜根定-1-甲醚（rubiadin-1-methylether，1），diadzein（2），臭矢菜素B（cleomiscosin B，3），臭矢菜素D（cleomiscosin D，4），异落叶松树脂醇（isolariciresinol，5），蒙花苷（linarin，6），异东莨菪香豆素（isoscopoletin，7），咖啡酸（caffic acid，8），香豆酸（coumaric acid，9），对羟基苯甲酸（p-hydroxyl-benzoic acid，10），齐墩果酸（11），乌苏酸（12），β-谷甾醇（13），胡萝卜苷（14），鸡屎藤苷（paederoside，15），鸡屎藤酸（paederosidic acid，16），鸡屎藤酸甲酯（paederosidic acid methyl ester，17），saprosmoside E（18），paederoscandoside（19），咖啡酸-4-O-β-D-吡喃葡萄糖苷（caffeic acid 4-O-β-D-glucopyranoside，20）。结论：化合物1-10，20均为首次从本植物中分离得到。

鸡矢藤化学成分研究

目的研究鸡矢藤Paederia scandense的化学成分.方法采用硅胶柱、Sephadex LH-20柱、ODS反相柱进行分离纯化,通过理化性质、光谱特征鉴定其结构.结果从鸡矢藤中分离到5个化合物,结构鉴定为茜根定-1-甲醚(1),胡萝卜苷(2),Borassoside E(3),异东莨菪香豆素(4),鸡矢藤苷(5).结论化合物3为首次从该植物中分离得到.

鸡屎藤与茶多酚联合干预大鼠结肠炎及脂肪肝研究

为了阐述结肠炎与脂肪肝发病的相互联系及鸡屎藤与茶多酚的联合干预作用,将40只SD大鼠随机分成A、B、C、D 4组,在全程高脂饲料条件下建立脂肪肝模型,C、D组再采用2,4,6-三硝基苯磺酸法建立并发性结肠炎病理模型,B、D组采用鸡屎藤和茶多酚干预。在第45天时断颈采血,检测血常规、转氨酶、血脂、血蛋白等指标变化。结果表明：鸡屎藤与茶多酚联合可激活嗜碱性粒细胞（P〉0.05）,降低脂肪肝伴发结肠炎时的血清内毒素水平（P〈0.05）,双向调节中性粒细胞数（P〉0．05或P〈0．05）及血小板数（P〉0．05），减轻内毒素对肝脏细胞的损伤。说明鸡屎藤与茶多酚可用于肠源性脂肪肝的联合防治。

鸡屎藤中环烯醚萜化合物的制备与鉴定

目的:本文对鸡屎藤95%乙醇提取物进行了化学成分研究.方法:采用D101-大孔树脂对环烯醚萜化合物进行富集,并对30%乙醇-水洗脱部分进行分离纯化,反复进行硅胶,ODS,Sephadex LH-20柱层析,通过理化常数和波谱分析进行化合物的结构鉴定.结果:分离到5个环烯醚萜化合物,鉴定为Saprosmoside E(1),鸡屎藤苷(paederoside,2),鸡屎藤酸(paederosidic acid,3),鸡屎藤酸甲酯(paederosidic acid methyl ester,4)以及去乙酰车叶草苷酸甲酯(deacetyl asperulosidic acid methyl ester,5).结论:其中去乙酰车叶草苷酸甲酯为首次从该植物中分离得到.

鸡矢藤纤维的开发应用初探

以茜草科植物鸡矢藤的茎秆为原料,通过浸渍结合碱煮脱胶的方法提取鸡矢藤纤维。采用组成成分测试、IR、SEM等测试与表征方法,对鸡矢藤纤维的组成成分和结构进行了分析和表征。进一步测试了鸡矢藤纤维的纺纱性能指标,分别为纤维索含量82.82%、比重1.323g/cm^3、长度41 mm、直径40.29μm、线密度2.93 dtex、单纤维强力55.4 cN、断裂强度18.9 cN/dtex、断裂伸长率2.87%。结果表明,所提取的鸡矢藤纤维组成成分和纺纱性能类似于麻类纤维,属于天然纤维素纤维,具有开发利用价值。

ICP-AES测定彝药鸡矢藤中的微量元素

为深入研究彝药药用功效,将鸡矢藤粉碎,用V(硝酸)+V(高氯酸)=3+1混合液进行湿法消解,用电感耦合等离子体发射光谱仪测定了鸡矢藤中各微量元素含量。结果表明,鸡矢藤中含有丰富的Fe、B、Mn、Sr、Zn、Cu等对人体有益的微量元素,其质量分数分别为Fe 308μg/g、B 180μg/g、Mn 48μg/g、Sr 26μg/g、Zn 22μg/g、Cu 8μg/g,不含Ni、Pb、As、Hg、Cd等对人体有害的元素。本结果为彝药研究提供实验依据。

鸡矢藤纤维的碱煮精制工艺

以鸡矢藤的茎秆为原料,通过浸渍、手工剥皮、浸酸和两次碱煮脱胶的方法精制鸡矢藤纤维。采用正交实验设计方法,优化得到鸡矢藤纤维最优脱胶精制工艺:第一次碱煮的碱浓度为1.4%,碱煮温度95℃,碱煮时间3.5 h;第二次碱煮的碱浓度为0.8%,碱煮温度95℃,碱煮时间2.5 h;浸酸浓度6%,室温浸酸0.5 h。以此工艺制得的精制鸡矢藤纤维性能指标均值为:纤维长度39.90 mm、纤维线密度2.85 dtex、强度18.56 cN/dtex、断裂伸长率2.81%。脱胶后的纤维具有较好的纺纱性能。