In this editorial,we commented on the article by Akers et al published in the recent issue of the World Journal of Clinical Cases.We focused specifically on the role of the transcription factor paired box protein 8(PA...In this editorial,we commented on the article by Akers et al published in the recent issue of the World Journal of Clinical Cases.We focused specifically on the role of the transcription factor paired box protein 8(PAX8)belonging to the family PAX in the carcinogenesis of a gynecologic tumor,endocervical adenocarcinoma,arising from the tissue of mesonephric origin,and the potential diagnostic value for the same type of neoplasms.The global vaccination program of human papillomavirus(HPV)has dramatically reduced the incidence of cervical cancer,including cases of adenocarcinoma.The type of adenoid epithelial origin has a lower frequency of HPV detection but tends to be more aggressive and fatal.Cases of endocervical adenocarcinoma occurring in females of menopause age have been described in the 2023 volume of the World Journal of Clinical Cases and in our study recently published in Oncol Lett.The histopathological findings and immunohistochemical assays showed that the lesions had glandular morphology,and the specimens in these two reports were immunohistochemically positive for the transcription factor PAX8,albeit that they had opposing expression profiles of tumor suppressor p16 and estrogen receptor and the presence of the HPV genome.The presence of a mucin protein,MUC 5AC,as revealed in both studies suggested target molecules for the diagnosis of mucinous adenoid type of uterine tumor and other histological origins.The clinical outcome was unfavorable due to metastasis and recurrence.This prompted the improvement of the antitumor modality,with the introduction of precise targeting therapy.Mucin has now been reported to be the therapeutic target for adenocarcinomas.展开更多
血管形成是胚胎发育期间的最早进程之一,其对胚胎的正常生长发育非常重要。血管形成和发育进程受一系列基因、转录因子、信号通路等调控。转录因子SOX7(SRY related high mobility group box 7)在调控心血管系统的发育、内皮细胞向造血...血管形成是胚胎发育期间的最早进程之一,其对胚胎的正常生长发育非常重要。血管形成和发育进程受一系列基因、转录因子、信号通路等调控。转录因子SOX7(SRY related high mobility group box 7)在调控心血管系统的发育、内皮细胞向造血细胞转化、动静脉分化等进程中发挥重要作用。其特异性高水平地表达于胚胎的心血管组织,提示其参与调控心血管的形成与发育。SOX7与众多参与调控血管内皮细胞的转录因子存在调节关系,其可通过抑制RUNX1的转录活性,抑制内皮细胞向造血细胞转化。Notch信号通路是调控血管发育和动静脉血管分化的关键通路,SOX7很可能作用于Notch信号通路上游,参与调控动脉和静脉血管的分化。但其调控血管发育的确切分子机制未来需进一步研究。展开更多
Objective:To explore the effect of"Yingxiang-Hegu"on Th1,Th2-related cytokines and[2]transcription factors T-bet and GATA-3 in rats with allergic rhinitis.Methods:Rats were randomly divided into three groups...Objective:To explore the effect of"Yingxiang-Hegu"on Th1,Th2-related cytokines and[2]transcription factors T-bet and GATA-3 in rats with allergic rhinitis.Methods:Rats were randomly divided into three groups:blank group,model group and acupoint group.The rat model of ovalbumin(OVA)AR was established,and the general condition of the rats was observed and scored.Acupuncture intervention was performed on the acupoint group on the second day after successful modeling,once per day for 20 min for 10 d.After intervention,the general behavior,behavioral score and histomorphological changes of nasal mucosa were observed.Eosinophils(EOS)were counted under microscope after nasal lavage smear staining,and the contents of total IgE,IFN-γ,IL-12,IL-4 and IL-5 in serum were detected by ELISA.Westernblot and IHC were used to detect the protein level and positive protein expression of specific transcription factors T-bet and GATA-3 in rat nasal mucosa.Results:After the establishment of the model,except for the blank group,the behavioral observation scores of rats in the model group and acupoint group were more than 5 points,indicating that the model was successful.After acupuncture intervention on acupoint"Yingxiang-Hegu",the behavioral score of rats in the acupoint group and western medicine group was significantly lower than that in the model group(P<0.05).Microscopic examination showed that the structure of nasal mucosa in the model group was obviously damaged,cilia were arranged discontinuously,uneven,local congestion and swelling,a large number of epithelial cells exfoliated and necrotic,goblet cell proliferation,obvious inflammatory cell infiltration.The pathological degree of nasal mucosa in the pair point group was significantly less than that in the model group.Compared with the model group,the levels of IFN-γ,IL-2 and IL-12 in serum were significantly increased,while IgE,IL-4,IL-5 and IL-6 were significantly decreased,GATA-3 protein and positive expression in nasal mucosa were significantly decreased and T-bet was significantly increased after acupuncture.Conclusion:Acupuncture at"Yingxiang-Hegu"can effectively improve the nasal sensitive symptoms and control nasal inflammation in AR rats.The mechanism may be that acupuncture at Yingxiang-Hegu can up-regulate the expression of T-bet,decrease the level of GATA-3,promote the production of Th1 cytokines and inhibit the synthesis and secretion of Th2 cells,thus restoring the immune balance of Th1 and Th2.展开更多
背景:Pax6基因是影响眼发育、分化的关键基因,获得过表达Pax6基因的稳定细胞株是研究其对骨髓间充质干细胞分化作用的首要任务,也是干细胞替代治疗的研究基础。目的:构建重组慢病毒载体Pax6-EGFP,体外转染人骨髓间充质干细胞,检测Pax6...背景:Pax6基因是影响眼发育、分化的关键基因,获得过表达Pax6基因的稳定细胞株是研究其对骨髓间充质干细胞分化作用的首要任务,也是干细胞替代治疗的研究基础。目的:构建重组慢病毒载体Pax6-EGFP,体外转染人骨髓间充质干细胞,检测Pax6基因在人骨髓间充质干细胞中的表达情况。方法:利用PCR法从人Pax6 cD NA基因克隆载体p GEM-PAX6上扩增Pax6目的片段,将其与XbaⅠ和BamHⅠ双酶切的慢病毒载体pH IV-EGFP连接,转化感受态细胞DH5α,挑取阳性克隆提取质粒,测序鉴定。经293T细胞包装后,收集含病毒颗粒的上清液,体外转染人骨髓间充质干细胞,同时设立阴性对照组(p HIV-EGFP空载体)。荧光显微镜下观察转染是否成功,Real time PCR检测转染细胞Pax6基因的表达。结果与结论:(1)酶切电泳及基因测序证实携带Pax6基因的慢病毒载体构建成功,包装后获得滴度为3×109 pfu/L的Pax6-EGFP重组载体;(2)转染人骨髓间充质干细胞后,在荧光显微镜下可以发出绿色荧光,且荧光强度不随培养时间延长而衰退;(3)经Real time PCR检测,被转染细胞内有Pax6基因表达,表明慢病毒转染是一种安全高效的基因修饰手段,能够使外源基因整合到宿主细胞基因组中。展开更多
文摘In this editorial,we commented on the article by Akers et al published in the recent issue of the World Journal of Clinical Cases.We focused specifically on the role of the transcription factor paired box protein 8(PAX8)belonging to the family PAX in the carcinogenesis of a gynecologic tumor,endocervical adenocarcinoma,arising from the tissue of mesonephric origin,and the potential diagnostic value for the same type of neoplasms.The global vaccination program of human papillomavirus(HPV)has dramatically reduced the incidence of cervical cancer,including cases of adenocarcinoma.The type of adenoid epithelial origin has a lower frequency of HPV detection but tends to be more aggressive and fatal.Cases of endocervical adenocarcinoma occurring in females of menopause age have been described in the 2023 volume of the World Journal of Clinical Cases and in our study recently published in Oncol Lett.The histopathological findings and immunohistochemical assays showed that the lesions had glandular morphology,and the specimens in these two reports were immunohistochemically positive for the transcription factor PAX8,albeit that they had opposing expression profiles of tumor suppressor p16 and estrogen receptor and the presence of the HPV genome.The presence of a mucin protein,MUC 5AC,as revealed in both studies suggested target molecules for the diagnosis of mucinous adenoid type of uterine tumor and other histological origins.The clinical outcome was unfavorable due to metastasis and recurrence.This prompted the improvement of the antitumor modality,with the introduction of precise targeting therapy.Mucin has now been reported to be the therapeutic target for adenocarcinomas.
文摘血管形成是胚胎发育期间的最早进程之一,其对胚胎的正常生长发育非常重要。血管形成和发育进程受一系列基因、转录因子、信号通路等调控。转录因子SOX7(SRY related high mobility group box 7)在调控心血管系统的发育、内皮细胞向造血细胞转化、动静脉分化等进程中发挥重要作用。其特异性高水平地表达于胚胎的心血管组织,提示其参与调控心血管的形成与发育。SOX7与众多参与调控血管内皮细胞的转录因子存在调节关系,其可通过抑制RUNX1的转录活性,抑制内皮细胞向造血细胞转化。Notch信号通路是调控血管发育和动静脉血管分化的关键通路,SOX7很可能作用于Notch信号通路上游,参与调控动脉和静脉血管的分化。但其调控血管发育的确切分子机制未来需进一步研究。
基金Lv Jingshan Acupuncture and Medicine Combination Technology Innovation Team(No.2019TD-003)Lv Jingshan Acupuncture and Moxibustion and Bioelectronic Medicine Innovation Team(No.2022TD-1006)。
文摘Objective:To explore the effect of"Yingxiang-Hegu"on Th1,Th2-related cytokines and[2]transcription factors T-bet and GATA-3 in rats with allergic rhinitis.Methods:Rats were randomly divided into three groups:blank group,model group and acupoint group.The rat model of ovalbumin(OVA)AR was established,and the general condition of the rats was observed and scored.Acupuncture intervention was performed on the acupoint group on the second day after successful modeling,once per day for 20 min for 10 d.After intervention,the general behavior,behavioral score and histomorphological changes of nasal mucosa were observed.Eosinophils(EOS)were counted under microscope after nasal lavage smear staining,and the contents of total IgE,IFN-γ,IL-12,IL-4 and IL-5 in serum were detected by ELISA.Westernblot and IHC were used to detect the protein level and positive protein expression of specific transcription factors T-bet and GATA-3 in rat nasal mucosa.Results:After the establishment of the model,except for the blank group,the behavioral observation scores of rats in the model group and acupoint group were more than 5 points,indicating that the model was successful.After acupuncture intervention on acupoint"Yingxiang-Hegu",the behavioral score of rats in the acupoint group and western medicine group was significantly lower than that in the model group(P<0.05).Microscopic examination showed that the structure of nasal mucosa in the model group was obviously damaged,cilia were arranged discontinuously,uneven,local congestion and swelling,a large number of epithelial cells exfoliated and necrotic,goblet cell proliferation,obvious inflammatory cell infiltration.The pathological degree of nasal mucosa in the pair point group was significantly less than that in the model group.Compared with the model group,the levels of IFN-γ,IL-2 and IL-12 in serum were significantly increased,while IgE,IL-4,IL-5 and IL-6 were significantly decreased,GATA-3 protein and positive expression in nasal mucosa were significantly decreased and T-bet was significantly increased after acupuncture.Conclusion:Acupuncture at"Yingxiang-Hegu"can effectively improve the nasal sensitive symptoms and control nasal inflammation in AR rats.The mechanism may be that acupuncture at Yingxiang-Hegu can up-regulate the expression of T-bet,decrease the level of GATA-3,promote the production of Th1 cytokines and inhibit the synthesis and secretion of Th2 cells,thus restoring the immune balance of Th1 and Th2.
文摘背景:Pax6基因是影响眼发育、分化的关键基因,获得过表达Pax6基因的稳定细胞株是研究其对骨髓间充质干细胞分化作用的首要任务,也是干细胞替代治疗的研究基础。目的:构建重组慢病毒载体Pax6-EGFP,体外转染人骨髓间充质干细胞,检测Pax6基因在人骨髓间充质干细胞中的表达情况。方法:利用PCR法从人Pax6 cD NA基因克隆载体p GEM-PAX6上扩增Pax6目的片段,将其与XbaⅠ和BamHⅠ双酶切的慢病毒载体pH IV-EGFP连接,转化感受态细胞DH5α,挑取阳性克隆提取质粒,测序鉴定。经293T细胞包装后,收集含病毒颗粒的上清液,体外转染人骨髓间充质干细胞,同时设立阴性对照组(p HIV-EGFP空载体)。荧光显微镜下观察转染是否成功,Real time PCR检测转染细胞Pax6基因的表达。结果与结论:(1)酶切电泳及基因测序证实携带Pax6基因的慢病毒载体构建成功,包装后获得滴度为3×109 pfu/L的Pax6-EGFP重组载体;(2)转染人骨髓间充质干细胞后,在荧光显微镜下可以发出绿色荧光,且荧光强度不随培养时间延长而衰退;(3)经Real time PCR检测,被转染细胞内有Pax6基因表达,表明慢病毒转染是一种安全高效的基因修饰手段,能够使外源基因整合到宿主细胞基因组中。
