The main symptoms of root rot,anthracnose,blight and damping-off of Panax notoginseng( Burk) F. H. Chen are introduced in the paper,and the corresponding control measures are elaborated from the aspects of agricultura...The main symptoms of root rot,anthracnose,blight and damping-off of Panax notoginseng( Burk) F. H. Chen are introduced in the paper,and the corresponding control measures are elaborated from the aspects of agricultural management measures,seed disinfection,seedbed treatment and chemical control.展开更多
[Objectives]This paper aimed to prepare derivatives of protopanaxadiol from Panax notoginseng(Burk.)FH Chen with acid anhydrides and study their anti-tumor activity.[Methods]The 3-hydroxyl group of protopanaxadiol was...[Objectives]This paper aimed to prepare derivatives of protopanaxadiol from Panax notoginseng(Burk.)FH Chen with acid anhydrides and study their anti-tumor activity.[Methods]The 3-hydroxyl group of protopanaxadiol was subjected to structural modification and reacted with acid anhydrides to prepare derivatives,in order to improve the anti-tumor activity of protopanaxadiol.None of the five compounds designed and synthesized had been reported in the literature,and they were novel compounds.The anti-tumor activity of the derivatives was studies using MTS method.Taking cisplatin and paclitaxel as positive control drugs,the bioactivity of the compounds 1-5 on anti-tumor cell lines(HL-60 cells,SMMC-7721 cells,A-549 cells,MCF-7 cells and SW480 cells)in vitro was screened.[Results]The compound 5 showed inhibitory effect on HL-60 cells,SMMC-7721 cells and A-549 cells.[Conclusions]The acid anhydride esterification method is simple to operate and easy to control.This study has reference value for the structural modification and anti-tumor activity research of protopanaxadiol from P.notoginseng(Burk.)FH Chen.展开更多
In the present study, we evaluated the genetic diversity of Panax notoginseng F H Chen, a domesticated species, and P. stipuleanatus H T Tsai et K M Feng, an endangered wild species in southeastern Yunnan and adjacent...In the present study, we evaluated the genetic diversity of Panax notoginseng F H Chen, a domesticated species, and P. stipuleanatus H T Tsai et K M Feng, an endangered wild species in southeastern Yunnan and adjacent areas in Vietnam, using sequences of the internal transcribed spacer (ITS) regions of nuclear ribosomal DNA and amplified fragment length polymorphism (AFLP) markers. Twenty-four accessions from three plantations of P. notoginseng and 51 samples from eight populations of P. stipuleanatus were assayed. A total of 694 bp of partial sequences of 18S, ITS 1, 5.8S, ITS2, and partial sequences of 26S were obtained. No sequence variation was detected within P. notoginseng and nine sites (1.30%) were variable in P. stipuleanatus. Two-thirds of the variable sites were found between Langqiao and other populations. In P. notoginseng, four pairs of AFLP primer combinations generated 312 bands, of which 240 (76.9%) were polymorphic and 60.15% of the polymorphisms were harbored within plantations. Approximately 41.0% and 66.9% of bands were polymorphic in population D7 and 5589, respectively. In P.stipuleanatus, the same four primer combinations produced 346 bands, of which 334 (96.5%) were polymorphic and approximately 62.14% of polymorphisms were maintained within populations. Considerable variations were observed. The percentage of polymorphic bands ranged from 50.2% to 84.9% and the average over populations was 70.9%. Cluster analysis did not show correlation of genetic differentiation with the distinctive leaf morphology of P. stipuleanatus (i.e. one form with bipinnatifid leaflets and the other with undivided leaflets). Because over 40% of genetic variations were maintained among populations and because of the very restricted distribution of P. stipuleanatus, all natural populations of this species should be conserved in situ. Considering that there are variations in P. notoginseng within and among plantations, we suggest establishing a genetic resource conservation garden or reintroducing P. notoginseng into its native habitats in southwestern China. Such reintroduction should be carefully executed after large-scale screening of genetic variation within the species.展开更多
Objective：Root or rhizome of Panax notoginseng（Sanqi）is known for its eutherapeutic effects.Panax vietnamensis var.fuscidicus,called Yesanqi or Yuenan sanqi by local residents,is also commercially available.They ar...Objective：Root or rhizome of Panax notoginseng（Sanqi）is known for its eutherapeutic effects.Panax vietnamensis var.fuscidicus,called Yesanqi or Yuenan sanqi by local residents,is also commercially available.They are similar in morphology,leading to serious safety problems in clinical medication.It is necessary to find the rapid and efficient methods to identify them.Methods：P.notoginseng and P.vietnamensis var.fuscidicus were identified by DNA barcoding based on the ITS2 sequence.Notoginsenoside R1 and ginsenosides（Rb1,Rg1,Re,Rd,Rc,and Rb2）were analyzed in the roots,fibrils,stems,leaves,and flowers of P.notoginseng and P.vietnamensis var.fuscidicus using high-performance liquid chromatography（HPLC）.Results：P.notoginseng and P.vietnamensis var.fuscidicus were separated into branches of divergent clusters,and P.vietnamensis var.fuscidicus and Panax vietnamensis were clustered into a clade with 98%similarity according to DNA barcoding analysis.The chemical compositions of P.notoginseng and P.vietnamensis var.fuscidicus were similar in roots;while their compositions and contents of notoginsenoside R1 and ginsenosides in flowers,leaves,stems,and fibrils were different.Conclusion：ITS2 is a rapid and efficient method to identify P.notoginseng and P.vietnamensis var.fuscidicus.HPLC analysis indicated that pharmacological action might be different between P.notoginseng and P.vietnamensis var.fuscidicus.展开更多
The effects of temperature and step-down relative humidity controlled hot-air drying(THC-HAD)on the drying kinetics,energy efficiency and quality,i.e.,rehydration ratio(RR),color parameters(L*,a*,b*),total color diffe...The effects of temperature and step-down relative humidity controlled hot-air drying(THC-HAD)on the drying kinetics,energy efficiency and quality,i.e.,rehydration ratio(RR),color parameters(L*,a*,b*),total color difference(ΔE*),Panax notoginseng saponins(PNS)content,and ginsenosides content(R1,Rg1,Re,Rd,Rb1)of Panax notoginseng roots were evaluated.The drying time was significantly affected by the drying temperature followed by the relative humidity(RH)of the drying air.Special combination of drying conditions,i.e.,drying temperature of 50°C,relative humidity of 40%for 3 h and then continuous dehumidification from 40%to 8%allowed to shorten the drying time by 25%compared to drying at the same temperature and continuous dehumidification.The longer was the drying time under constant high RH of drying air,the lower was the RR of dried samples.The step-down RH strategy contributed to the formation of a porous structure,enhancement of drying efficiency and quality improvement.Generally,the ginsenosides content increased with the increase in temperature,while no obvious trend was recorded for ginsenoside R1.The contents of the ginsenoside R1,Rg1,Rb1 and PNS decreased with the increase in the drying time under constant high RH.Taking into account the drying time,energy consumption and quality attributes,drying at the temperature of 50°C,constant RH of 40%for 3 h and then step-down RH from 40%to 8%was proposed as the most favorable combination of drying conditions for dehydration of whole Panax notoginseng roots.展开更多
文摘The main symptoms of root rot,anthracnose,blight and damping-off of Panax notoginseng( Burk) F. H. Chen are introduced in the paper,and the corresponding control measures are elaborated from the aspects of agricultural management measures,seed disinfection,seedbed treatment and chemical control.
基金Supported by Science&Technology Department of Yunnan Province-Kunming Medical University Joint Fund for Applied Basic Research[2017FE468(-001)]NSFC-Yunnan Joint Fund[U1502226].
文摘[Objectives]This paper aimed to prepare derivatives of protopanaxadiol from Panax notoginseng(Burk.)FH Chen with acid anhydrides and study their anti-tumor activity.[Methods]The 3-hydroxyl group of protopanaxadiol was subjected to structural modification and reacted with acid anhydrides to prepare derivatives,in order to improve the anti-tumor activity of protopanaxadiol.None of the five compounds designed and synthesized had been reported in the literature,and they were novel compounds.The anti-tumor activity of the derivatives was studies using MTS method.Taking cisplatin and paclitaxel as positive control drugs,the bioactivity of the compounds 1-5 on anti-tumor cell lines(HL-60 cells,SMMC-7721 cells,A-549 cells,MCF-7 cells and SW480 cells)in vitro was screened.[Results]The compound 5 showed inhibitory effect on HL-60 cells,SMMC-7721 cells and A-549 cells.[Conclusions]The acid anhydride esterification method is simple to operate and easy to control.This study has reference value for the structural modification and anti-tumor activity research of protopanaxadiol from P.notoginseng(Burk.)FH Chen.
基金国家重点基础研究发展计划(973计划)，中国科学院知识创新工程项目，国家自然科学基金，the Outstanding Overseas Chinese Grant of the Chinese Academy of Sciences, and the U.S. National Science Foundation Supported by，the U.S. National Science Foundation
文摘In the present study, we evaluated the genetic diversity of Panax notoginseng F H Chen, a domesticated species, and P. stipuleanatus H T Tsai et K M Feng, an endangered wild species in southeastern Yunnan and adjacent areas in Vietnam, using sequences of the internal transcribed spacer (ITS) regions of nuclear ribosomal DNA and amplified fragment length polymorphism (AFLP) markers. Twenty-four accessions from three plantations of P. notoginseng and 51 samples from eight populations of P. stipuleanatus were assayed. A total of 694 bp of partial sequences of 18S, ITS 1, 5.8S, ITS2, and partial sequences of 26S were obtained. No sequence variation was detected within P. notoginseng and nine sites (1.30%) were variable in P. stipuleanatus. Two-thirds of the variable sites were found between Langqiao and other populations. In P. notoginseng, four pairs of AFLP primer combinations generated 312 bands, of which 240 (76.9%) were polymorphic and 60.15% of the polymorphisms were harbored within plantations. Approximately 41.0% and 66.9% of bands were polymorphic in population D7 and 5589, respectively. In P.stipuleanatus, the same four primer combinations produced 346 bands, of which 334 (96.5%) were polymorphic and approximately 62.14% of polymorphisms were maintained within populations. Considerable variations were observed. The percentage of polymorphic bands ranged from 50.2% to 84.9% and the average over populations was 70.9%. Cluster analysis did not show correlation of genetic differentiation with the distinctive leaf morphology of P. stipuleanatus (i.e. one form with bipinnatifid leaflets and the other with undivided leaflets). Because over 40% of genetic variations were maintained among populations and because of the very restricted distribution of P. stipuleanatus, all natural populations of this species should be conserved in situ. Considering that there are variations in P. notoginseng within and among plantations, we suggest establishing a genetic resource conservation garden or reintroducing P. notoginseng into its native habitats in southwestern China. Such reintroduction should be carefully executed after large-scale screening of genetic variation within the species.
基金supported by the grants from the National Natural Science Foundation of China(No.81603238)Beijing Nova Project(No.xx2018014)the Major Science and Technology Programs in Yunnan Province(No.2016ZF001-001)
文摘Objective：Root or rhizome of Panax notoginseng（Sanqi）is known for its eutherapeutic effects.Panax vietnamensis var.fuscidicus,called Yesanqi or Yuenan sanqi by local residents,is also commercially available.They are similar in morphology,leading to serious safety problems in clinical medication.It is necessary to find the rapid and efficient methods to identify them.Methods：P.notoginseng and P.vietnamensis var.fuscidicus were identified by DNA barcoding based on the ITS2 sequence.Notoginsenoside R1 and ginsenosides（Rb1,Rg1,Re,Rd,Rc,and Rb2）were analyzed in the roots,fibrils,stems,leaves,and flowers of P.notoginseng and P.vietnamensis var.fuscidicus using high-performance liquid chromatography（HPLC）.Results：P.notoginseng and P.vietnamensis var.fuscidicus were separated into branches of divergent clusters,and P.vietnamensis var.fuscidicus and Panax vietnamensis were clustered into a clade with 98%similarity according to DNA barcoding analysis.The chemical compositions of P.notoginseng and P.vietnamensis var.fuscidicus were similar in roots;while their compositions and contents of notoginsenoside R1 and ginsenosides in flowers,leaves,stems,and fibrils were different.Conclusion：ITS2 is a rapid and efficient method to identify P.notoginseng and P.vietnamensis var.fuscidicus.HPLC analysis indicated that pharmacological action might be different between P.notoginseng and P.vietnamensis var.fuscidicus.
基金supported in part by the Hebei Province Key Research and Development Project(Grant No.203777119D,19227210D)in part by the Scientific Research Projects of Universities in Hebei Province(Grant No.ZD2021056)in part by the Hebei Province College and Middle School Students Science and Technology Innovation Ability Cultivation Project(Grant No.2021H060505)and part by China Agriculture Research System of MOF and MARA(CARS-21).
文摘The effects of temperature and step-down relative humidity controlled hot-air drying(THC-HAD)on the drying kinetics,energy efficiency and quality,i.e.,rehydration ratio(RR),color parameters(L*,a*,b*),total color difference(ΔE*),Panax notoginseng saponins(PNS)content,and ginsenosides content(R1,Rg1,Re,Rd,Rb1)of Panax notoginseng roots were evaluated.The drying time was significantly affected by the drying temperature followed by the relative humidity(RH)of the drying air.Special combination of drying conditions,i.e.,drying temperature of 50°C,relative humidity of 40%for 3 h and then continuous dehumidification from 40%to 8%allowed to shorten the drying time by 25%compared to drying at the same temperature and continuous dehumidification.The longer was the drying time under constant high RH of drying air,the lower was the RR of dried samples.The step-down RH strategy contributed to the formation of a porous structure,enhancement of drying efficiency and quality improvement.Generally,the ginsenosides content increased with the increase in temperature,while no obvious trend was recorded for ginsenoside R1.The contents of the ginsenoside R1,Rg1,Rb1 and PNS decreased with the increase in the drying time under constant high RH.Taking into account the drying time,energy consumption and quality attributes,drying at the temperature of 50°C,constant RH of 40%for 3 h and then step-down RH from 40%to 8%was proposed as the most favorable combination of drying conditions for dehydration of whole Panax notoginseng roots.
