Panax quinquefolius L.(American ginseng)and Panax ginseng C.A.Meyer are famous herbal medicines.Accurate authentication of the species has grown to be a significant impact.This study aims to develop a PCR kit for the ...Panax quinquefolius L.(American ginseng)and Panax ginseng C.A.Meyer are famous herbal medicines.Accurate authentication of the species has grown to be a significant impact.This study aims to develop a PCR kit for the authentication of Panax quinquefolius L.and Panax ginseng based on the nuclear internal transcribed spacer 2(ITS2)gene with the improved PCR-restriction fragment length polymorphism(PCR-RFLP)method.The reagent components in the development work were prepared and determined by the kit consisting of the DNA extraction,PCR amplification,and restriction enzyme digestion systems.A total of 21 batches of Panax quinquefolius L.and Panax ginseng samples collected from different areas were validated.Their specificity,stability,and repeatability were evaluated.The purity of genomic DNA extracted was 1.73±0.13 according to the ratio of A_(260)/A_(280),and the mass concentration was 3.15±0.22μg/g(using the kit).PCR amplicons of Panax quinquefolius L.and Panax ginseng were 122 bp in length.After the PCR products were digested by restriction enzyme Hinf I,a distinct pattern exhibited in the species of Panax quinquefolius L.with two fragments of 40 bp and 80 bp respectively,whereas those from Panax ginseng in addition to adulterated samples could not.Evaluation confirmed that the DNA kit results were stable and repeatable after 10,15,and 20 freeze-thaw cycles:the evaluation was 100%specific.The DNA kit proposed in the study can be used for the identification of Panax quinquefolius L.展开更多
American ginseng, Panax quinquefolius L., is an herbaceous perennial species that is destructively harvested for its bioactive compounds called ginsenosides. The demand for this herb fosters illegal poaching and over-...American ginseng, Panax quinquefolius L., is an herbaceous perennial species that is destructively harvested for its bioactive compounds called ginsenosides. The demand for this herb fosters illegal poaching and over-harvesting that reduces genetic variability and population viability. Five wild populations in western North Carolina were studied to better understand the production of ginsenosides in leaf and root tissues. Total ginsenoside concentration was significantly higher in leaves than roots, though total yield was higher in roots due to greater root biomass. However, some ginsensosides (Rb2, Rd and Re) had higher or more consistent yields in leaves than roots, so might be developed into a sustainable source of these medicinally-active compounds. Additionally, we identified regional root chemotypes that differed in the production of the ginsenosides Rg1 and Re and could be developed into regional cultivars depending on the desired panel of ginsenosides.展开更多
One new triterpcnoid saponin, quinquenoside L17 （1）, was isolated from the leaves and stems of Panax quinquefolium L., and its structure was elucidated as 20-O-[（β-D-xylopyranosyl-（1-6）-O-β-D-glucopyranosy）]-6...One new triterpcnoid saponin, quinquenoside L17 （1）, was isolated from the leaves and stems of Panax quinquefolium L., and its structure was elucidated as 20-O-[（β-D-xylopyranosyl-（1-6）-O-β-D-glucopyranosy）]-6-O-β-D-glucopyranosy1-dammar-24-ene- 3,6,12,20-tetraol, by the combination analysis of one-dimensional NMR and two-dimensional NMR, mass spectrometry, CD spectrum and chemical evidences.展开更多
基金The present project was financially funded by Jilin Provincial Department of Education,China(JJKH20180377KJ)Jilin Provincial Department of Science and Technology,China(20190304108YY,20200404152YY,20200403047SF)TCM Science and Technology Project of Jilin Province,China(2019132).
文摘Panax quinquefolius L.(American ginseng)and Panax ginseng C.A.Meyer are famous herbal medicines.Accurate authentication of the species has grown to be a significant impact.This study aims to develop a PCR kit for the authentication of Panax quinquefolius L.and Panax ginseng based on the nuclear internal transcribed spacer 2(ITS2)gene with the improved PCR-restriction fragment length polymorphism(PCR-RFLP)method.The reagent components in the development work were prepared and determined by the kit consisting of the DNA extraction,PCR amplification,and restriction enzyme digestion systems.A total of 21 batches of Panax quinquefolius L.and Panax ginseng samples collected from different areas were validated.Their specificity,stability,and repeatability were evaluated.The purity of genomic DNA extracted was 1.73±0.13 according to the ratio of A_(260)/A_(280),and the mass concentration was 3.15±0.22μg/g(using the kit).PCR amplicons of Panax quinquefolius L.and Panax ginseng were 122 bp in length.After the PCR products were digested by restriction enzyme Hinf I,a distinct pattern exhibited in the species of Panax quinquefolius L.with two fragments of 40 bp and 80 bp respectively,whereas those from Panax ginseng in addition to adulterated samples could not.Evaluation confirmed that the DNA kit results were stable and repeatable after 10,15,and 20 freeze-thaw cycles:the evaluation was 100%specific.The DNA kit proposed in the study can be used for the identification of Panax quinquefolius L.
文摘American ginseng, Panax quinquefolius L., is an herbaceous perennial species that is destructively harvested for its bioactive compounds called ginsenosides. The demand for this herb fosters illegal poaching and over-harvesting that reduces genetic variability and population viability. Five wild populations in western North Carolina were studied to better understand the production of ginsenosides in leaf and root tissues. Total ginsenoside concentration was significantly higher in leaves than roots, though total yield was higher in roots due to greater root biomass. However, some ginsensosides (Rb2, Rd and Re) had higher or more consistent yields in leaves than roots, so might be developed into a sustainable source of these medicinally-active compounds. Additionally, we identified regional root chemotypes that differed in the production of the ginsenosides Rg1 and Re and could be developed into regional cultivars depending on the desired panel of ginsenosides.
基金supported by 973 Program(No.2006CB708517)Program for New Century Excellent Talents in University of Peoples Republic of China(No.NCET-04-0289)
文摘One new triterpcnoid saponin, quinquenoside L17 （1）, was isolated from the leaves and stems of Panax quinquefolium L., and its structure was elucidated as 20-O-[（β-D-xylopyranosyl-（1-6）-O-β-D-glucopyranosy）]-6-O-β-D-glucopyranosy1-dammar-24-ene- 3,6,12,20-tetraol, by the combination analysis of one-dimensional NMR and two-dimensional NMR, mass spectrometry, CD spectrum and chemical evidences.
