The mutagenic and carcinogenic effects of parathion-methyl were examined by bacterial reverse mutation assay and a long-term experiment with wistar rats. The potential mutagenic effect of parathion-methyl in Salmonell...The mutagenic and carcinogenic effects of parathion-methyl were examined by bacterial reverse mutation assay and a long-term experiment with wistar rats. The potential mutagenic effect of parathion-methyl in Salmonella typhimurium TA100 bacterial cells was observed without rat liver S9 metabolic activation. Parathion-methyl was further investigated for pathological changes in rat pancreas and liver. The long-term rat experiments showed that parathion-methyl exposure for 3 months can cause pathological changes in rat pancreases acinar cells and pancreatic hepatocytes. Atypical acinar cell focuses (AACF) were determined in the liver and pancreas of the rats. The results from short-term Ames test and long-term rat experiments suggested that parathion-methyl would be potential carcinogenic.展开更多
A study on the sorption of monocrotophos and parathion methyl on Yellow River's sediments was performed. The influence of some factors, such as pH value and ionic strength, on the sorption was investigated and f...A study on the sorption of monocrotophos and parathion methyl on Yellow River's sediments was performed. The influence of some factors, such as pH value and ionic strength, on the sorption was investigated and first order kinetics mode for the sorption of monocrophos was shown. Freundlich isotherm was suggested as the most appropriate type for monocrophos and parathion methyl. But the sorption isotherms of monocrotophos and parathion methyl are also in conformity with Langmuir equation. Both partition and adsorption occur to the sorption of monocrophos to Yellow River's sediments, but partition is the predominant for that of parathion methyl.展开更多
A novel sensor for the determination of parathion-methyl based on couple grafting of functional molecular imprinted polymers (MIPs) was fabricated which is developed by anchoring the MIP layer on surfaces of silica ...A novel sensor for the determination of parathion-methyl based on couple grafting of functional molecular imprinted polymers (MIPs) was fabricated which is developed by anchoring the MIP layer on surfaces of silica particles embedded CdSe quantum dots by surface imprinting technology. The synthesized molecular imprinted silica nanospheres (CdSe@SiO2@MIP) allow a high selectivity and sensitivity of parathion-methyl via fluorescence intensity decreasing when the MIP material rebinding the parathion-methyl molecule. Compared with the MIP fabri- cated in traditional method, the template of parathion-methyl was easier to remove from the CdSe@SiO2@MIP imprinted material. Under optimal conditions, the fluorescence intensity of parathion-methyl at the imprinted sensor was detected by spectrofluorophotometer. The relative fluorescence intensity of CdSe@SiO2@MIP decreased linearly with the increasing concentration of parathion-methyl ranging from 0.013 mg·kg^-1 to 2.63mg·kg^-1 with a detection limit (3σ) of 0.004 mg·kg^-1 (S/N=3), which is lower than the MIP in tradition. The imprinted film sensor was applied to detect parathion-methyl in vegetable samples without the interference of other organophosphate pesticides and showed a prosperous application in the field of food safety.展开更多
A new facile fluorescence probing strategy, which was based on N-doped carbon dots(NCDs) and methyl parathion hydrolase(MPH), was developed for the determination of parathion-methyl(PM). The fluorescence intensi...A new facile fluorescence probing strategy, which was based on N-doped carbon dots(NCDs) and methyl parathion hydrolase(MPH), was developed for the determination of parathion-methyl(PM). The fluorescence intensity of NCDs-MPH system was proportional to PM concentration in the range of 2.38–73.78 mmol/L, with a detection limit of 0.338 mmol/L. Moreover, the present simple and facile method could be used to determine methyl parathion in environmental and agricultural samples successfully.Furthermore, the detection mechanism of this system is inner filter effect and molecular interactions between NCDs and p-nitrophenol, which is the hydrolysis product of PM catalyzed by methyl parathion hydrolase.展开更多
文摘The mutagenic and carcinogenic effects of parathion-methyl were examined by bacterial reverse mutation assay and a long-term experiment with wistar rats. The potential mutagenic effect of parathion-methyl in Salmonella typhimurium TA100 bacterial cells was observed without rat liver S9 metabolic activation. Parathion-methyl was further investigated for pathological changes in rat pancreas and liver. The long-term rat experiments showed that parathion-methyl exposure for 3 months can cause pathological changes in rat pancreases acinar cells and pancreatic hepatocytes. Atypical acinar cell focuses (AACF) were determined in the liver and pancreas of the rats. The results from short-term Ames test and long-term rat experiments suggested that parathion-methyl would be potential carcinogenic.
文摘A study on the sorption of monocrotophos and parathion methyl on Yellow River's sediments was performed. The influence of some factors, such as pH value and ionic strength, on the sorption was investigated and first order kinetics mode for the sorption of monocrophos was shown. Freundlich isotherm was suggested as the most appropriate type for monocrophos and parathion methyl. But the sorption isotherms of monocrotophos and parathion methyl are also in conformity with Langmuir equation. Both partition and adsorption occur to the sorption of monocrophos to Yellow River's sediments, but partition is the predominant for that of parathion methyl.
基金Project supported by the Science & Technology Commission of Shanghai Municipality (No. 10JC1404000) and the Fundamental Research Funds for the Central Universities. This work was also supported by the Natural Science Foundation of Shanghai (No. 11ZR1410700) and the Funding from Shanghai Key Laboratory of Green Chemistry and Chemical Processes of ECNU.Abbreviations used MIPs, molecularly imprinted polymers EGDMA, ethylene glycol dimethacrylate+5 种基金 PBS, phosphate buffer QDs, quantum dots TEOS, tetraethylorthosilicate APTS, 3-aminopropyltriethoxysilane EDC, 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride NHS, N-bydroxysuccinimide
文摘A novel sensor for the determination of parathion-methyl based on couple grafting of functional molecular imprinted polymers (MIPs) was fabricated which is developed by anchoring the MIP layer on surfaces of silica particles embedded CdSe quantum dots by surface imprinting technology. The synthesized molecular imprinted silica nanospheres (CdSe@SiO2@MIP) allow a high selectivity and sensitivity of parathion-methyl via fluorescence intensity decreasing when the MIP material rebinding the parathion-methyl molecule. Compared with the MIP fabri- cated in traditional method, the template of parathion-methyl was easier to remove from the CdSe@SiO2@MIP imprinted material. Under optimal conditions, the fluorescence intensity of parathion-methyl at the imprinted sensor was detected by spectrofluorophotometer. The relative fluorescence intensity of CdSe@SiO2@MIP decreased linearly with the increasing concentration of parathion-methyl ranging from 0.013 mg·kg^-1 to 2.63mg·kg^-1 with a detection limit (3σ) of 0.004 mg·kg^-1 (S/N=3), which is lower than the MIP in tradition. The imprinted film sensor was applied to detect parathion-methyl in vegetable samples without the interference of other organophosphate pesticides and showed a prosperous application in the field of food safety.
基金supported by the National Natural Science Foundation of China(No.21207057)the Fundamental Research Funds for the Central Universities(No.lzujbky-201643)
文摘A new facile fluorescence probing strategy, which was based on N-doped carbon dots(NCDs) and methyl parathion hydrolase(MPH), was developed for the determination of parathion-methyl(PM). The fluorescence intensity of NCDs-MPH system was proportional to PM concentration in the range of 2.38–73.78 mmol/L, with a detection limit of 0.338 mmol/L. Moreover, the present simple and facile method could be used to determine methyl parathion in environmental and agricultural samples successfully.Furthermore, the detection mechanism of this system is inner filter effect and molecular interactions between NCDs and p-nitrophenol, which is the hydrolysis product of PM catalyzed by methyl parathion hydrolase.