Interactions of primary insult biomechanics and secondary cascades in spinal cord injury： implications for therapy

The complex and variable nature of traumatic spinal cord inju- ry （SCI） presents a unique challenge for translational research. SCI is not bound by any demographic nor is it limited to specific injury biomechanics.

Enhancing the Photosensitivity of Hypocrellin A by Perylene Diimide Metallacage‑Based Host–Guest Complexation for Photodynamic Therapy

The development of supramolecular hosts which can efficiently encapsulate photosensitizers to improve the photodynamic efficacy holds great promise for cancer therapy.Here,we report two perylene diimide-based metallacages that can form stable host–guest complexes with planar conjugated molecules including polycyclic aromatic hydrocarbons and photosensitizers(hypocrellin A).Such host–guest complexation not only prevents the aggregation of photosensitizers in aqueous environments,but also offers fluorescence resonance energy transfer(FRET)from the metallacage to the photosensitizers to further improve the singlet oxygen generation(Φ_(Δ)=0.66).The complexes are further assembled with amphiphilic polymers,forming nanoparticles with improved stability for anticancer study.Both in vitro and in vivo studies indicate that the nanoparticles display excellent anticancer activities upon light irradiation,showing great potential for cancer photodynamic therapy.This study provides a straightforward and effective approach for enhancing the photosensitivity of conventional photosensitizers via host–guest complexation-based FRET,which will open a new avenue for host–guest chemistry-based supramolecular theranostics.

Research on the Effect of Dance Therapy on Improving Social Communication Ability of Children with Autism

Research motivation:Through the 12 weeks dance therapy intervention for children with autism,the purpose is to explore the intervention model of dance therapy for children with autism and the changes in motor ability,social ability,and communication ability of children with autism after dance therapy intervention.The results of the research are expected to expand the intervention mode of dance therapy in my country and provide practical reference for rehabilitation intervention of children with autism.Research methods:24 autistic boys aged 6 to 12 with mild to moderate symptoms were recruited and screened through the Internet as the subjects of this study.We randomly divided them into experimental group(N=12)and control group(N=12).All children with autism have an autism diagnosis certificate issued by Children’s Hospital or a tertiary hospital,excluding other mental diseases(such as epilepsy,major physical disability,mental illness,no history of drugs and other interventions,etc.).We used the paired sample t-test to compare the score difference between the dance treatment group and the control group before and after the two groups,and used the observation method to record the basic communication behavior and the number of active communication behaviors in the experimental group during the intervention process.All data analysis is used in SPSS 20.0.Research results:After 12 weeks of dance therapy intervention,there were statistically significant differences in the gross movement,balance,and coordination abilities of the children in the experimental group compared with those before the intervention(p<0.01).There was no significant difference between the children in the control group(p>0.05).After 12 weeks of dance therapy intervention,there were statistically significant differences in the scores of the social response scale for children with autism in the experimental group(p<0.05).There was no significant change in the scores of each item of the SRS scale before and after intervention in the control group and the dance treatment group(p>0.05).

AAV mediated carboxyl terminus of Hsp70 interacting protein overexpression mitigates the cognitive and pathological phenotypes of APP/PS1 mice

The E3 ubiquitin ligase,carboxyl terminus of heat shock protein 70(Hsp70)interacting protein(CHIP),also functions as a co-chaperone and plays a crucial role in the protein quality control system.In this study,we aimed to investigate the neuroprotective effect of overexpressed CHIP on Alzheimer’s disease.We used an adeno-associated virus vector that can cross the blood-brain barrier to mediate CHIP overexpression in APP/PS1 mouse brain.CHIP overexpression significantly ameliorated the performance of APP/PS1 mice in the Morris water maze and nest building tests,reduced amyloid-βplaques,and decreased the expression of both amyloid-βand phosphorylated tau.CHIP also alleviated the concentration of microglia and astrocytes around plaques.In APP/PS1 mice of a younger age,CHIP overexpression promoted an increase in ADAM10 expression and inhibitedβ-site APP cleaving enzyme 1,insulin degrading enzyme,and neprilysin expression.Levels of HSP70 and HSP40,which have functional relevance to CHIP,were also increased.Single nuclei transcriptome sequencing in the hippocampus of CHIP overexpressed mice showed that the lysosomal pathway and oligodendrocyte-related biological processes were up-regulated,which may also reflect a potential mechanism for the neuroprotective effect of CHIP.Our research shows that CHIP effectively reduces the behavior and pathological manifestations of APP/PS1 mice.Indeed,overexpression of CHIP could be a beneficial approach for the treatment of Alzheimer’s disease.

Design of Urban Parent-Child Interaction Landscape Spaces:A Case Study of Wuhan Overseas Chinese Town(OCT) Park

From the perspective of the design of parent-child interaction landscapes,this paper took Wuhan Overseas Chinese Town (OCT) Park for example,explored children’s physiology,psychology and emotions as well as external factors,translated them into building landscape spaces,children’s emotion spaces,children’s exploration spaces,children’s social spaces,so as to facilitate parent-child interactions in these spaces,thereby to enhance the family-care education and parent-child emotional exchanges,and provide references for the construction of parent-child interaction spaces.

Complex interactions between microRNAs and hepatitis B/C viruses

MicroRNAs(miRNAs) are a class of small noncoding RNAs that post-transcriptionally regulate the expression of many target genes via mRNA degradation or translation inhibition. Many studies have shown that miRNAs are involved in the modulation of gene expression and replication of hepatitis B virus(HBV) and hepatitis C virus(HCV) and play a pivotal role in host-virus interactions. Increasing evidence also demonstrates that viral infection leads to alteration of the miRNA expression profile in hepatic tissues or circulation. The deregulated miRNAs participate in hepatocellular carcinoma(HCC)initiation and progression by functioning as oncogenes or tumor suppressor genes by targeting various genes involved in cancer-related signaling pathways. The distinct expression pattern of miRNAs may be a useful marker for the diagnosis and prognosis of virus-related diseases considering the limitation of currently used biomarkers. Moreover, the role of deregulated miRNA in host-virus interactions and HCC development suggested that miRNAs may serve as therapeutic targets or astools. In this review, we summarize the recent findings about the deregulation and the role of miRNAs during HBV/HCV infection and HCC development, and we discuss the possible mechanism of action of miRNAs in the pathogenesis of virus-related diseases. Furthermore, we discuss the potential of using miRNAs as markers for diagnosis and prognosis as well as therapeutic targets and drugs.

Systematic screening reveals synergistic interactions that overcome MAPK inhibitor resistance in cancer cells

Objective:Effective adjuvant therapeutic strategies are urgently needed to overcome MAPK inhibitor(MAPKi)resistance,which is one of the most common forms of resistance that has emerged in many types of cancers.Here,we aimed to systematically identify the genetic interactions underlying MAPKi resistance,and to further investigate the mechanisms that produce the genetic interactions that generate synergistic MAPKi resistance.Methods:We conducted a comprehensive pair-wise sgRNA-based high-throughput screening assay to identify synergistic interactions that sensitized cancer cells to MAPKi,and validated 3 genetic combinations through competitive growth,cell viability,and spheroid formation assays.We next conducted Kaplan-Meier survival analysis based on The Cancer Genome Atlas database and conducted immunohistochemistry to determine the clinical relevance of these synergistic combinations.We also investigated the MAPKi resistance mechanisms of these validated synergistic combinations by using co-immunoprecipitation,Western blot,qRTPCR,and immunofluorescence assays.Results:We constructed a systematic interaction network of MAPKi resistance and identified 3 novel synergistic combinations that effectively targeted MAPKi resistance(ITGB3+IGF1R,ITGB3+JNK,and HDGF+LGR5).We next analyzed their clinical relevance and the mechanisms by which they sensitized cancer cells to MAPKi exposure.Specifically,we discovered a novel protein complex,HDGF-LGR5,that adaptively responded to MAPKi to enhance cancer cell stemness,which was up-or downregulated by the inhibitors of ITGB3+JNK or ITGB3+IGF1R.Conclusions:Pair-wise sgRNA library screening provided systematic insights into elucidating MAPKi resistance in cancer cells.ITGB3-+IGF1R-targeting drugs(cilengitide+linsitinib)could be used as an effective therapy for suppressing the adaptive formation of the HDGF-LGR5 protein complex,which enhanced cancer stemness during MAPKi stress.

Fundamental problems in rehabilitation robots based on neuro-machine interaction

Study results in the last decades show that amount and quality of physical exercises,then the active participation,and now the cognitive involvement of patient in rehabilitation training are crucial to enhance recovery outcome of motor dysfunction patients after stroke.Rehabilitation robots mainly have been developed along this direction to satisfy requirements of recovery therapy,or focused on one or more of the above three points.Therefore,rehabilitation robot based on neuro-machine interaction has been proposed for the paralyzed limb training of post-stroke patient,which utilizes motor related EEG,UCSDI(Ultrasound Current Source Density Imaging),EMG for the robot control and feeds back the multi-sensory interaction information such as visual,auditory,force,haptic sensation to the patient simultaneously.This neuro-controlled and perceptual rehabilitation robot will bring great benefits to post-stroke patients.In order to develop such a kind of rehabilitation robot,some key technologies,such as non-invasive precise measurement and decoding of neural signals,realistic sensation feedback,coordinated control for both the rehabilitation robot and the patient,need to be solved.In this paper,some fundamental problems in developing and optimizing such a kind of rehabilitation robot based on neuro-machine interaction are proposed and discussed.

受体相互作用蛋白激酶1调节癌症进展和免疫反应的研究现状

受体相互作用蛋白激酶1(receptor-interacting protein kinase 1,RIPK1)是一种多结构域丝氨酸/苏氨酸蛋白激酶。它通过磷酸化特定的蛋白质,引起下游的信号转导和生物效应。近年来,随着对RIPK1的深入研究,学者发现其在自身免疫性疾病、神经退行性疾病,以及多种实体瘤和血液肿瘤中具有重要意义。一方面,RIPK1通过激活特定通路如核因子-κB(nuclear factor-κB,NF-κB)和丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)等促进细胞存活及炎症反应。另一方面,RIPK1通过与胱天蛋白酶-8(cysteinyl aspartate specific proteinase-8,caspase-8)作用促进凋亡,或与RIPK3和混合谱系激酶结构域样假激酶(mixed lineage kinase domain-like protein,MLKL)作用促进坏死性凋亡的发生。RIPK1作为上游信号在不同肿瘤患者中表达水平不同。其支架功能和激酶活性可以调节癌症进展,也可以启动机体适应性免疫,抑制肿瘤进展;此外,还能产生免疫抑制性肿瘤微环境而促进肿瘤的发展。其双重作用在调节癌症的发生、发展及机体免疫反应方面都有所展现,可以作为新的治疗靶点控制癌症进展。该文从RIPK1的结构入手,深入探讨其功能,特别是其在调节癌症进展和免疫反应方面的功能,为癌症靶向药物的开发提供新的思路。

基于虚拟增强现实交互技术的茶疗养生文旅产业研究

以茶疗养生为主题,以虚拟增强现实交互(augmented reality,AR)技术为科技手段和技术优势,通过创新驱动发展实现茶疗养生文旅场景设计应用的新突破。在现实场景下,用户可使用具备AR功能的各种智能设备,通过识别物理空间中的标识物触发AR应用,从而实现茶疗养生信息及时推送,实现现实场景与虚拟信息之间的交互,从而达到信息补充连贯及情景增强效果,使用户构建起茶疗养生的整体观念。通过解析茶疗养生文旅产业的研发思路,丰富茶疗养生信息体系的承载维度,探索实现茶疗养生文旅产业迭代的新途径。

互动仪式:青少年戏剧治疗小组的服务机制探析

戏剧疗法是近年来兴起的一种以人为本的治疗方法,尝试以更灵活、创新、隐喻的手段,缓解或解决服务对象面临的心理与社会问题。基于“问题青少年”戏剧治疗小组的社会工作介入服务行动实践,本研究以互动仪式理论为视角,探讨其治疗过程的实现机制。社会工作戏剧治疗的核心是以文本、空间和技术作为共同关注的行动焦点,将自省、改变和希望作为情感能量的来源,并通过戏剧治疗过程中共享的情感状态的交互强化,使青少年获得正向的自我认知,缓解家庭关系,从而促进青少年成长问题的解决。实现这一过程的机制涉及信任构建、防御消解、焦点制造、情感递进等关键环节。为避免在治疗过程中出现情感能量此消彼长及权力支配等问题,需要注意以正向符号延长情感状态,以优势视角化解青少年的抵触心理,在互动仪式过程中寻找和解决问题而非进行道德评判,以及通过强调内观自我价值来消解权力支配。

环境媒介与具身交互:艺术疗愈空间的热点与趋势

在人们情绪压力不断增大的当今社会,艺术疗愈以其创造性特征和非语言媒介帮助疗愈对象改善心理或身体状态,旨在讨论艺术疗愈空间中的环境媒介与疗愈过程中的具身交互关系、设计实践与价值。借助CiteSpace可视化软件分析近十年来的重要文献,揭示艺术疗愈的研究现状;运用文献计量学方法,探索环境媒介在艺术疗愈空间的应用,定义环境媒介的具身性、主体间性、沉浸性特征及三者间的关联;以具身交互理论为线索,结合设计案例分析疗愈空间中环境媒体的具身性实践与作用方式,以期进一步探索结合新兴技术的环境媒介未来在我国的应用发展。艺术疗愈空间的环境媒介能具身地影响疗愈对象的交互行为与认知,提高疗愈的效率和质量。环境媒介与具身交互能增进疗愈过程中的不同主体间的体验共享,并辅助疗愈对象沉浸地参与或融入艺术疗愈;数字技术的发展不断地丰富环境媒介的形式,提高艺术疗愈的效果。

治疗性聆听对孤独症谱系障碍儿童社会交往功能的疗效

目的 观察治疗性聆听对孤独症谱系障碍(ASD)儿童在社会交往功能方面的影响。方法 2022年1月至2023年1月,北京博爱医院ASD儿童40例随机分为A组(n=20)和B组(n=20),两组均接受基础康复治疗和常规作业治疗,B组在常规作业治疗的同时进行治疗性聆听训练,共8周。治疗前后采用残疾儿童功能评定量表(PEDI)、儿童孤独症评定量表(CARS)、孤独症行为量表(ABC)、Peabody运动发育量表第2版(PDMS-2)和加拿大作业活动行为评估(COPM)进行评定。结果 治疗后,两组各项指标均明显改善(|t|> 3.194, P <0.01),除ABC评分外,B组各项指标均优于A组(|t|>2.122, P <0.05)。结论 在常规作业治疗的基础上进行治疗性聆听训练,可以更有效地改善ASD儿童的感觉处理障碍和社会交往功能。

肿瘤免疫微环境与治疗互作的机制研究进展

肿瘤免疫微环境在肿瘤的发生和发展过程中扮演着关键角色,并与治疗的长期有效性密切相关。值得注意的是,除了受微环境影响,治疗的同时也会反过来主动塑造微环境的构成。近年来,借助单细胞测序等新兴技术,各种治疗手段与免疫微环境之间的相互作用以及其对治疗效果的影响在多种肿瘤中得到揭示。本文综述了原发性肿瘤免疫微环境对肿瘤疗效的影响,介绍了不同治疗方式对微环境的重塑情况,并探讨了继发性免疫微环境与肿瘤相互作用的复杂调节机制对最终治疗结局的影响。厘清肿瘤免疫微环境与治疗互作的机制对治疗效果的监控和预测,以及更优化治疗方案的制定具有重要意义。

SPEEDO:一款快速准确的碳离子蒙特卡罗剂量计算程序

目的:开发一款用于碳离子治疗的快速准确的蒙特卡罗程序SPEEDO。方法:对于电磁过程,采用连续慢化近似的II类浓缩历史模拟方案,模拟能量歧离、射程歧离、多重散射和电离过程。对于核相互作用,本研究考虑5种靶核:氢、碳、氮、氧和钙。产生的次级带电粒子遵循同样的浓缩历史框架。本研究模拟碳离子在4种材料(水、软组织、肺和骨头)中的输运过程,并将计算的剂量结果与放射治疗物理专用蒙特卡罗模拟软件TOPAS进行对比验证,最后与在武威重离子治疗中心医用重离子加速器示范装置(HIMM-WW)上测得的水箱剂量进行对比。结果:SPEEDO的模拟结果与TOPAS具有良好的一致性。对于每种材料在物理剂量大于其体素最大剂量点10%的体素区域,两者的相对最大剂量平均误差均小于2%。在治疗能量为400 MeV/u时,SPEEDO的计算时间(13.8 min)明显小于TOPAS的计算时间(105.0 min)。SPEEDO的计算结果与HIMM-WW的测量值在横向剂量分布和积分剂量深度曲线方面展现出良好的一致性。结论:SPEEDO程序可以准确快速地实现碳离子的蒙特卡罗剂量计算。

五行虚拟现实技术联合睡眠认知行为疗法治疗心肾不交证慢性失眠临床研究

目的:观察五行虚拟现实(VR)技术联合睡眠认知行为疗法(CBT-I)治疗心肾不交证慢性失眠的疗效。方法:选取60例慢性失眠心肾不交证患者,按随机数字表法分别为试验组及对照组各30例。对照组予以CBT-I,试验组在对照组基础上采用五行VR技术治疗。2组均治疗4周。比较2组临床疗效,比较2组治疗前后低频功率(LF)、高频功率(HF)、低频功率与高频功率之比(LF/HF)、匹兹堡睡眠质量指数(PSQI)、中医证候积分、汉密顿抑郁量表-17(HAMD-17)、汉密尔顿焦虑量表(HAMA)评分的变化。结果:试验组总有效率为66.67%,对照组为16.67%,2组比较,差异有统计学意义(P<0.05)。治疗4周后,试验组LF指标值、试验组LF/HF指标值、对照组LF/HF指标值治疗前后比较,差异有统计学意义(P<0.05);治疗后,试验组LF、LF/HF指标值均低于对照组(P<0.05)。治疗4周后,试验组睡眠质量、入睡时间、睡眠效率、催眠药物、日间功能障碍评分及总分低于对照组(P<0.05)。治疗4周后,2组中医证候积分、HAMA、HAMD-17评分均较治疗前下降(P<0.05),试验组中医证候积分、HAMA、HAMD-17评分均低于对照组(P<0.05)。结论:五行VR技术联合CBT-I治疗心肾不交证慢性失眠疗效较好,可改善自主神经中副交感神经及平衡性,提升睡眠质量。

基于“脑肠互动”探讨通腑法治疗缺血性中风的机制

缺血性中风是我国居民致残的首要病因,治疗选择仍然有限。通腑法在缺血性中风治疗中已被证明具有显著疗效,但其作用机制尚不清楚。随着脑肠互动研究的进展,越来越多的证据表明脑肠互动在缺血性中风中起着重要作用。文章以“脑肠互动”为理论基础探讨了通腑法治疗缺血性中风的作用机理,从肠道菌群、脑肠肽、肠道代谢产物、肠道粘膜屏障等多个方面进行讨论阐述,总结出通腑法可以通过调节肠道菌群、脑肠肽含量、肠道代谢产物含量及修护肠道粘膜屏障等治疗缺血性中风,以期为阐明通腑法治疗缺血性中风的作用机制提供更多思路,具有重要的理论及临床价值。

药物联合应用对中草药相关肝损伤的影响

随着中医药在全球范围内的广泛使用和临床中药物联用情况的增多,中草药相关肝损伤(HILI)和药物安全性事件频发,这给中药新药研发和中医产业发展带来了重大挑战。目前,在系统归纳总结药物联用对HILI的影响,梳理其临床特征、发生过程和互作机制,尤其是研究中西药联用后的药效动力学和药代毒理学过程等方面,仍然存在较大不足。亟需进一步构建系统整合的研究体系,特别是在靶标分子、细胞间交流、组织间串扰和体内毒性评价等方面,开展更加精准、特异和早期的研究。通过临床与基础并行的病证基础研究,提出符合中医药理论和应用规律的HILI防控策略,将为提升病证场景下的中西药物联用水平提供基础支撑和可信证据。

交互式健康教育联合运动疗法对腹膜透析患者运动功能、自护能力及生活质量的影响

目的探讨交互式健康教育联合运动疗法对腹膜透析患者运动功能、自护能力及生活质量的影响。方法选取2021年6月至2023年6月潜江市中心医院收治的80例行腹膜透析治疗患者为研究对象,采用随机数表法将其分为对照组、观察组,每组各40例,对照组予以常规护理措施,观察组在上述护理基础上加用交互式健康教育联合运动疗法,比较两组患者干预前后运动功能[6 min步行试验(6MWT)、10次坐立秒数]、自护能力(ESCA)及生活质量(SF-36)评分。结果干预后,两组患者6MWT、10次坐立秒数显著改善,且观察组6MWT高于对照组,10次坐立秒数少于对照组,差异有统计学意义(P<0.05)。干预前后,两组ESCA、SF-36量表各维度评分及总分评分显著改善,且观察组上述指标高于对照组,差异有统计学意义(P<0.05)。结论交互式健康教育联合运动疗法有助于改善腹膜透析患者运动功能,提高自我护理能力,提升生活质量,值得临床推广应用。

Cell-cell interactions and communication dynamics in lung fibrosis

Cell-cell interactions are essential components of coordinated cell function in lung homeostasis.Lung diseases involve altered cell-cell interactions and communication between different cell types,as well as between subsets of cells of the same type.The identification and understanding of intercellular signaling in lung fibrosis offer insights into the molecular mechanisms underlying these interactions and their implications in the development and progression of lung fibrosis.A comprehensive cell atlas of the human lung,established with the facilita-tion of single-cell RNA transcriptomic analysis,has enabled the inference of intercellular communications using ligand-receptor databases.In this review,we provide a comprehensive overview of the modified cell-cell commu-nications in lung fibrosis.We highlight the intricate interactions among the major cell types within the lung and their contributions to fibrogenesis.The insights presented in this review will contribute to a better understand-ing of the molecular mechanisms underlying lung fibrosis and may guide future research efforts in developing targeted therapies for this debilitating disease.