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Primary Comments on Chemotaxonomy of Paris spp. Based on Saponins Analysis 被引量:6
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作者 黄芸 王强 崔力剑 《Journal of Chinese Pharmaceutical Sciences》 CAS 2005年第3期176-180,共5页
Aim The several species of the genus Paris called "Chonglou" are famous traditional Chinese herbal medicines. We established the quantitative analysis method of the steroidal saponins in some species of the genus Pa... Aim The several species of the genus Paris called "Chonglou" are famous traditional Chinese herbal medicines. We established the quantitative analysis method of the steroidal saponins in some species of the genus Paris and discussed their relations. Methods We detected the contents of 11 steroidal saponins in Paris samples with a Kromasel C18 ( 150 mm× 4.6 mm ID, 5μm) column which was subjected to gradient elution with acetonitrile-water (30:70- 60:40, V/V) at a flow rate of 1 mL· min^-1 by HPLC-ELSD and established chemical cluster tree using SPSS 11 software. Results All the samples could be separated and calibration curves of 11 saponins were prepared. We successfully detected the contents of 11 steroidal saponins in 14 Paris spp. in 30 min. The recovery for the assay of saponins was between 95 % and 97 %. The RSD of precision of 11 saponins and stability of samples were below 3 %. Chemical phylogenetic tree based on saponin contents indicated that 17 samples of Paris spp. clustered separately. Conclusion The established method is accurate and convenient, and suitable for the quantitative analysis of these 11 steroidal saponins in Paris spp.. The chemical phylogenetic tree is in accordance with Takhtajian classical taxonomy. 展开更多
关键词 paris steroidal saponin cluster analysis HPLC-ELSD
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Parisaponin Ⅰ和Parisvietnaside A的NMR数据分析 被引量:5
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作者 刘奕训 康利平 +6 位作者 赵玉 陈合兵 邹鹏 熊呈琦 赵阳 谭大维 马百平 《波谱学杂志》 CAS CSCD 北大核心 2009年第3期316-326,共11页
从滇重楼中分离得到2个甾体皂苷,利用1H NMR、13C NMR、1H-1H COSY、HSQC、HMBC、1D和2D TOCSY等多种核磁共振方法鉴定其结构分别为(25R)-26-O-β-D-吡喃葡萄糖基-3β,22α,26-三羟基-呋甾-5-烯-3-O-α-L-吡喃鼠李糖基-(1→2)-[α-L-呋... 从滇重楼中分离得到2个甾体皂苷,利用1H NMR、13C NMR、1H-1H COSY、HSQC、HMBC、1D和2D TOCSY等多种核磁共振方法鉴定其结构分别为(25R)-26-O-β-D-吡喃葡萄糖基-3β,22α,26-三羟基-呋甾-5-烯-3-O-α-L-吡喃鼠李糖基-(1→2)-[α-L-呋喃阿拉伯糖基-(1→4)]-β-D-吡喃葡萄糖苷(1,Parisaponin I)和(25R)-3β,5α,6β-三羟基-△7-螺甾烯-3-O-β-D-吡喃葡萄糖基-(1→3)-[α-L-吡喃鼠李糖基-(1→2)]-β-D-吡喃葡萄糖苷(2,paris-vietnaside A).对化合物1和2的1H NMR和13C NMR信号分别进行了归属和详细分析,并纠正了文献中的核磁数据归属错误.化合物2为首次从滇重楼中分离得到. 展开更多
关键词 核磁共振(NMR) 归属 2D NMR 甾体皂苷 滇重楼
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Paris Saponin Ⅰ Induces G_2/M Cell Cycle Arrest and Apoptosis in Human Gastric Carcinoma SGC7901 Cells 被引量:4
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作者 萧梅芳 戴霞红 +5 位作者 贺新春 周蓉蓉 章保新 胡关胜 黄泽炳 范学工 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2011年第6期768-772,共5页
The aim of this study was to investigate the effect of Paris saponin I (PSI ) on human gastric carcinoma cell growth and apoptosis and to explore the potential mechanisms. The proliferation of SGC7901 cells was moni... The aim of this study was to investigate the effect of Paris saponin I (PSI ) on human gastric carcinoma cell growth and apoptosis and to explore the potential mechanisms. The proliferation of SGC7901 cells was monitored by the MTT cell viability assay, while the nuclear morphology of apoptotic cells was assessed by Hoechst 33258 staining. Flow cytometry was performed to analyze the cell cycle progression of propidium iodide (PI)-stained SGC7901 cells and the apoptotic rate of annexin V/PI-stained ceils. Western blotting was used to examine the expression of several cell cycle proteins, including cyclin 131 and Cdkl, and the apoptosis-regulated proteins Bcl-2, Bax, cytochrome c, procaspase-9, and procaspase-3. The MTT assay demonstrated that PSI could induce significant dose- and time-dependent inhibition of SGC7901 cell proliferation. Marked morphological changes, including condensation of chromatin, nuclear fragmentation and apoptotic bodies were clearly shown on Hoechst 33258 staining. PS I treatment also resulted in the disruption of the cell cycle at Gz/M and the induction of apoptosis. Following PSI treatment, the cell cycle-related proteins cyclin B 1 and Cdkl were down- regulated. Expression of the pro-apoptotic protein Bax was increased, while anti-apoptotic protein Bcl-2 decreased. PSI treatment resulted in elevated cytoplasmic cytochrome c and activation of the apoptotic proteases caspase-9 and caspase-3. These data indicate that PS I acts as an inhibitor of proliferation in SGC7901 cells by inducing cell cycle arrest and mitochondria-dependent apoptosis. PSI is a potential therapeutic agent against human gastric carcinoma. 展开更多
关键词 paris saponin I human gastric carcinoma cell cycle APOPTOSIS
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A new spirostanol saponin from the rhizomes of Paris mairei 被引量:3
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作者 Xiao Xiao Liu Lei Wang +1 位作者 Ting Ting Zhang Qiang Wang 《Chinese Chemical Letters》 SCIE CAS CSCD 2009年第7期820-822,共3页
A new spirostanol steroidal saponin, named maireioside A (1), together with three known steroidal saponins, hypoglaucin G (2), parisaponin I (3), and diosgenin-3-O-α-L-rhamnopyranosyl (1→4)-[α-L-rhamnopyran... A new spirostanol steroidal saponin, named maireioside A (1), together with three known steroidal saponins, hypoglaucin G (2), parisaponin I (3), and diosgenin-3-O-α-L-rhamnopyranosyl (1→4)-[α-L-rhamnopyranosyl (1→2)]-β-D-glucopyranoside (4), were isolated from the rhizomes of Paris mairei. The structure elucidation was accomplished by 1D and 2D NMR methods, HR-ESI-MS, and hydrolysis. C 2009 Qiang Wang. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved. 展开更多
关键词 Spirostanol saponin paris mairei Maireioside A
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Two New Highly Oxygenated Spirostanol Saponins from Paris polyphylla var.stenophylla 被引量:2
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作者 Ling-Yu Jin Ting-Xiang Lu +6 位作者 Xu-Jie Qin Wei Ni Huan Yan Yu Chen Hui Liu Hong-Ping He Hai-Yang Liu 《Natural Products and Bioprospecting》 CAS 2016年第4期205-210,共6页
Phytochemical investigation of the rhizomes of Paris polyphylla var.stenophylla led to the isolation of two new highly oxygenated spirostanol saponins,named paristenosides A(1)and B(2),together with seven known compou... Phytochemical investigation of the rhizomes of Paris polyphylla var.stenophylla led to the isolation of two new highly oxygenated spirostanol saponins,named paristenosides A(1)and B(2),together with seven known compounds.Their structures were established mainly on the base of NMR spectroscopic techniques and mass spectrometry,as well as chemical methods.In addition,the cytotoxicity of the two new saponins was tested.Graphical Abstract Two new highly oxygenated spirostanol saponins,paristenosides A(1)and B(2),were isolated from the rhizomes of Paris polyphylla var.stenophylla.Their structures were established mainly based on NMR spectroscopic techniques and mass spectrometry,as well as chemical methods. 展开更多
关键词 paris polyphylla var.stenophylla LILIACEAE Spirostanol saponins paristenoside A paristenoside B
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Determination of the Content of Total Saponins in Paris daliensis H.Li et V. G. Soukoup and Paris dulongensis H. Li et S. kurita 被引量:4
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作者 Zhongjie HUANG Haiyan XIANG +3 位作者 Shaoshan ZHANG Mengjie YU Yuan LIU Hao ZHANG 《Medicinal Plant》 2017年第1期17-19,共3页
[Objectives]To determine the content of total saponins in Paris daliensis H. Li et V. G. Soukoup and Paris dulongensis H. Li et S.kurita,fill the gap in the study of Paris daliensis H. Li et V. G. Soukoup and Paris du... [Objectives]To determine the content of total saponins in Paris daliensis H. Li et V. G. Soukoup and Paris dulongensis H. Li et S.kurita,fill the gap in the study of Paris daliensis H. Li et V. G. Soukoup and Paris dulongensis H. Li et S. kurita,and lay a foundation for searching new medicinal resources of Paris L. [Methods]The perchloric acid colorimetric method and UV spectrophotometer were used to determine the content of total saponins in Paris daliensis H. Li et V. G. Soukoup and Paris dulongensis H. Li et S. kurita at 406 nm. [Results]The lowest content of total saponins in Paris daliensis H. Li et V. G. Soukoup was 2. 637%,while the highest content reached 5. 474%; the lowest content of total saponins in Paris dulongensis was 1. 806%,while the highest content reached 3. 532%. Through the methodological examination,it was found that this method was stable and reliable. [Conclusions] The content of total saponins was high in Paris daliensis H.Li et V. G. Soukoup and Paris dulongensis H. Li et S. kurita,and it was suggested to further study and find the medicinal resources of Paris L. 展开更多
关键词 paris daliensis H.Li ET V.G.Soukoup paris dulongensis H.Li ET S.kurita UV SPECTROPHOTOMETRY Total saponinS Content de termination
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Contrastive Study on Content and Anti-oxidation Activity of Total Saponins from Cultivated and Wild Paris Plants in Southwest China 被引量:3
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作者 Mengjie YU Haiyan XIANG +2 位作者 Shaoshan ZHANG Zhongjie HUANG Yuan LIU 《Medicinal Plant》 2017年第1期7-11,共5页
[Objectives]To study content of total saponins from cultivated and wild Paris herbs in Southwest China,evaluate its in vitro antioxidation activity,and explore the correlation between quality of Paris herbs and produc... [Objectives]To study content of total saponins from cultivated and wild Paris herbs in Southwest China,evaluate its in vitro antioxidation activity,and explore the correlation between quality of Paris herbs and production place,variety,cultivated and wild varieties.[Methods]Colorimetric method of perchloric acid reaction was used,and content of total saponins from Paris herbs was determined by ultraviolet spectrophotometer at 406 nm. [Results]The lowest content of total saponins from cultivated and wild Paris herbs in Southwest China was0. 55%,while the maximum was 12. 24%,with obvious difference. Total saponins from Paris herbs had stronger clearance ability on free radical DPPH and stronger reduction ability on Fe^(3+),and the results showed good dose-effect relationship with concentration. Among them,S6 had the highest clearance ability on DPPH,which reached 89. 66%. In the determination of reduction ability,the absorbance of S16 reached3. 643 and was the highest,with very good reducing property. Content and anti-oxidation capacity of total saponins from Paris herbs in Southwest China did not have obvious correlation with production place,altitude and cultivation but was related to variety. [Conclusions]The quality of Paris herbs in Southwest China was unstable,and their anti-oxidation abilities were also different,and cultivation could not guarantee high quality. It should normalize cultivation,and develop planting industry of Paris herbs,which could guarantee stable supply of Paris herbs and stability and controllability of quality. 展开更多
关键词 CULTIVATED and WILD paris herbs UV-VISIBLE spectrophotometry Total saponins ANTI-OXIDATION
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南重楼Paris vietnamensis活性物质的分离与鉴定 被引量:24
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作者 黄芸 崔力剑 +1 位作者 王强 叶文才 《药学学报》 CAS CSCD 北大核心 2006年第4期361-364,共4页
目的研究南重楼Paris vietnamensis (Takht.)中具有细胞毒活性的甾体皂苷类化学成分。方法采用柱色谱进行分离纯化,通过光谱分析和理化方法鉴定化合物结构,并进行体外细胞毒活性筛选。结果从南重楼中分离得到11个甾体皂苷类化合物... 目的研究南重楼Paris vietnamensis (Takht.)中具有细胞毒活性的甾体皂苷类化学成分。方法采用柱色谱进行分离纯化,通过光谱分析和理化方法鉴定化合物结构,并进行体外细胞毒活性筛选。结果从南重楼中分离得到11个甾体皂苷类化合物,化合物1结构为:3β,5α,6α-三羟基-7(8)-烯-异螺甾烷醇-3-氧-β-D-葡吡喃糖基(1→3)[α-L-鼠李吡喃糖基(1→2)]-β-D-葡吡喃糖苷(1),命名为南重楼皂苷A。化合物2—11分别为:25(R)-薯蓣皂苷元-3-氧-α-L-阿拉伯呋喃糖基(1→4)]-β-D-葡吡喃糖苷(2);25(R)薯蓣皂苷元-3-氧-α-L-鼠李糖吡喃基(1→2)-β-D-葡吡喃糖苷(3);25(R)薯蓣皂苷元-3-氧-α-L-阿拉伯呋喃糖基(1→4)[α—L-鼠李吡喃糖基-(1→2)]-β-D-葡吡喃糖苷(4);25(R)薯蓣皂苷元-3-氧-α-L-葡吡喃糖基(1→3)[α—L-鼠李吡喃糖基-(1→2)]-β-D-葡吡喃糖苷(5);25(R)薯蓣皂苷元-3-氧-α-L-鼠李吡喃糖基(1→4)]-α-L-鼠李吡喃糖基(1→4)[-α-L-鼠李吡喃糖基(1→2)]-β-D-葡吡喃糖苷(6);25(R)偏诺苷元-3-氧-α-L-阿拉伯呋喃糖基(1→4)]-β-D-葡吡喃糖苷(7);25(R)偏诺苷元-3-氧-α-L-鼠李吡喃糖基(1→2)]-β-D-葡吡喃糖苷(8);25(R)偏诺苷元-3-氧-α-L-阿拉伯呋喃糖基(1→4)]-α-L-鼠李吡喃糖基(1→2)]-β-D-葡吡喃糖苷(9);25(R)偏诺皂苷元-3-氧-α-L-葡吡喃糖基(1→3)[α-L-鼠李吡喃糖基(1→2)]-β-D-葡吡喃糖苷(10);25(R)偏诺苷元-3-氧-α-L-鼠李吡喃糖基(1→4)-α-L-鼠李吡喃糖基(1→4)-α-L-鼠李吡喃糖基(1→2)]-β-D-葡吡喃糖苷(11),体外筛选表明部分甾体皂苷类化合物具有细胞毒作用。结论化合物1为一新化合物,化合物2,3,6—11均为首次从南重楼中获得。化合物3,4,6,8具有对肝癌HepG2和胃癌SGC-7901的细胞毒活性。 展开更多
关键词 南重楼 甾体皂苷 异螺甾烷醇类化合物 南重楼皂苷A 细胞毒
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Comprehensive Evaluation on Impacts of Diseases on Quality of Paris polyphylla var. chinensis Based on Ultraviolet and Infrared Spectra 被引量:1
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作者 Yongxia KE Jixiu SHEN +3 位作者 Hairong ZHONG Risha WEIZE Yunzhang XU Yuan LIU 《Medicinal Plant》 CAS 2019年第3期63-65,68,共4页
[Objectives] The research aimed to evaluate impacts of diseases on quality of Paris polyphylla var. chinensis. [Methods]Ultraviolet spectrophotometry was used to determine and contrast content of total saponins in roo... [Objectives] The research aimed to evaluate impacts of diseases on quality of Paris polyphylla var. chinensis. [Methods]Ultraviolet spectrophotometry was used to determine and contrast content of total saponins in root,stem and leaf from healthy and diseased plants. Infrared characteristic absorption peaks of healthy and diseased plants were found by infrared fingerprint method for comparative analysis.[Results]Total saponins content in root sample J4 of healthy plant reached 4. 89% and was the highest,while it was 0. 13% in stem sample J11 of healthy plant and was the lowest. Total saponins content in root sample B1 of diseased plant was 1. 68% and was the highest,while it was 0. 1% in stem sample B7 of diseased plant and was the lowest. In healthy and diseased plants,total saponins content in root was significantly higher than that in stem and leaf. Content of total saponins in root from healthy plant was significantly higher than that from diseased plant,and there was little difference in stem and leaf of healthy and diseased plants. Root sample Jg of healthy plant had obvious C-O characteristic vibration of polysaccharides and glycosides and absorption peak of O-H bending vibration in steroidal saponin near 1 160. 02 cm-1,while diseased plant had no obvious absorption peak. Near 861. 97 and 768. 07 cm-1,root sample Jg of healthy plant had significant C-C stretching vibration in sugar ring and characteristic absorption peak of characteristic vibration of steroidal saponin,while root sample Bg of diseased plant had no obvious characteristic peak. [Conclusions] Diseases significantly affected composition and content of total saponins and steroidal saponins from P. polyphylla var. chinensis,further affecting quality of P. polyphylla var. chinensis. 展开更多
关键词 paris polyphylla VAR. CHINENSIS ULTRAVIOLET Infrared Total saponinS QUALITY
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氮磷钾配方施肥提高林下七叶一枝花产量和重楼皂苷含量
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作者 苏海兰 郑梅霞 +2 位作者 陈宏 朱雁鸣 朱育菁 《植物营养与肥料学报》 CAS CSCD 北大核心 2024年第1期128-136,共9页
【目的】研究氮、磷、钾不同组合用量对林下栽培七叶一枝花产量及重楼皂苷含量的影响,为七叶一枝花人工栽培合理施肥提供参考。【方法】采用“3414”试验设计,利用福建南平杉竹混交林下4年生七叶一枝花(Paris polyphylla Smith var.chin... 【目的】研究氮、磷、钾不同组合用量对林下栽培七叶一枝花产量及重楼皂苷含量的影响,为七叶一枝花人工栽培合理施肥提供参考。【方法】采用“3414”试验设计,利用福建南平杉竹混交林下4年生七叶一枝花(Paris polyphylla Smith var.chinensis (Franch.) Hara)进行田间试验,将氮、磷、钾肥当地适宜水平定为“2”,分别为N 270 kg/hm^(2)、P_(2)O_(5) 375 kg/hm^(2)、K_(2)O 300 kg/hm^(2),不足“1”和过量“3”水平分别为适宜水平的0.5、1.5倍。在七叶一枝花收获时期,测定其植株形态、根茎产量、4种重楼皂苷含量等指标,并计算生产效益。【结果】氮、磷、钾肥用量均显著影响七叶一枝花的植株形态、根茎产量和重楼皂苷含量,对七叶一枝花根茎产量的提升率分别为59.75%、27.54%、63.15%,以钾肥的贡献率最高,其次为氮肥,磷肥较低。N1水平下,增加磷、钾水平显著降低七叶一枝花的株高、茎粗和叶长,N3水平七叶一枝花的株高、叶长均达到最高,但茎粗低于N1和N2水平,表明适宜氮肥用量对七叶一枝花的生长至关重要。七叶一枝花的根茎产量和总重楼皂苷含量还受肥料交互效应的影响。当N、P_(2)O_(5)、K_(2)O用量分别为270、375、450 kg/hm^(2) (N_(2)P_(2)K_3)和270、375、300 kg/hm^(2)(N_(2)P_(2)K_(2))时,根茎产量达到最高,分别为2804、2648 kg/hm^(2)。N_(2)P_(2)K_3的总重楼皂苷含量也最大,达到1.62%,显著高于其他处理。施氮磷钾肥净增收效益在N_(2)P_(2)K_3处理下最高,N_(2)P_(2)K_(2)处理次之。【结论】适量的氮、磷肥配合较高的钾肥可显著提升七叶一枝花根茎产量和总重楼皂苷含量,提高七叶一枝花的经济效益。因此,在供试地区,推荐N、P_(2)O_(5)、K_(2)O用量分别为270、375、450 kg/hm^(2)。 展开更多
关键词 七叶一枝花 “3414”设计 植株形态 根茎产量 重楼皂苷 经济效益 氮磷钾组合
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不同炮制方法对续断中总皂苷和川续断皂苷Ⅵ含量的影响 被引量:7
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作者 张丹 曹纬国 陶燕铎 《重庆医科大学学报》 CAS CSCD 北大核心 2010年第7期1054-1057,共4页
目的:研究续断生品及不同炮制品中总皂苷与川续断皂苷Ⅵ的含量变化,探讨不同炮制方法对续断中总皂苷和川续断皂苷Ⅵ含量的影响。方法:采用分光光度法测定续断生品及炮制品中总皂苷的含量;采用高效液相色谱法测定川续断皂苷Ⅵ的含量。结... 目的:研究续断生品及不同炮制品中总皂苷与川续断皂苷Ⅵ的含量变化,探讨不同炮制方法对续断中总皂苷和川续断皂苷Ⅵ含量的影响。方法:采用分光光度法测定续断生品及炮制品中总皂苷的含量;采用高效液相色谱法测定川续断皂苷Ⅵ的含量。结果:与续断生品相比较,各炮制品的总皂苷含量略有增加;但炮制后川续断皂苷Ⅵ的含量增加显著。结论:不同炮制方法均能影响续断中川续断皂苷Ⅵ的含量变化,证明了传统炮制方法的合理性。 展开更多
关键词 炮制方法 总皂苷 川续断皂苷 分光光度法 高效液相色谱法
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重庆产区不同居群续断中总皂苷与川续断皂苷Ⅵ的含量分析 被引量:4
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作者 曹纬国 陶燕铎 +1 位作者 张丹 王刚 《时珍国医国药》 CAS CSCD 北大核心 2011年第2期332-334,共3页
目的测定重庆产区不同居群续断中总皂苷与川续断皂苷Ⅵ的含量,探讨其在居群间的含量差异。方法以香草醛-冰醋酸-高氯酸为显色体系,采用分光光度法测定续段中总皂苷的含量;采用HPLC法测定续断中川续断皂苷Ⅵ的含量。结果各居群续断均能... 目的测定重庆产区不同居群续断中总皂苷与川续断皂苷Ⅵ的含量,探讨其在居群间的含量差异。方法以香草醛-冰醋酸-高氯酸为显色体系,采用分光光度法测定续段中总皂苷的含量;采用HPLC法测定续断中川续断皂苷Ⅵ的含量。结果各居群续断均能达到药典规定的药用标准,但不同居群续断中皂苷类成分的含量差异较大,以武隆产续断中皂苷成分含量较高。结论实验方法适用于续断的质量分析,可以为续断的质量评价提供参考。 展开更多
关键词 分光光度法 高效液相色谱 川续断皂苷 总皂苷
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HPLC测定损伤复元颗粒中川续断皂苷Ⅵ的含量 被引量:2
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作者 吴旭彤 毛春芹 +2 位作者 陆兔林 左文 胡俊扬 《南京中医药大学学报》 CAS CSCD 北大核心 2011年第6期585-586,共2页
目的为有效控制损伤复元颗粒的质量,建立制剂中川续断皂苷Ⅵ的HPLC定量测定方法。方法 Kromasil C18柱(250mm×4.6mm,5μm);流动相乙腈-水(28∶72);检测波长212nm;体积流量1.0mL/min;柱温30℃。结果川续断皂苷Ⅵ在0.104~0.936mg/m... 目的为有效控制损伤复元颗粒的质量,建立制剂中川续断皂苷Ⅵ的HPLC定量测定方法。方法 Kromasil C18柱(250mm×4.6mm,5μm);流动相乙腈-水(28∶72);检测波长212nm;体积流量1.0mL/min;柱温30℃。结果川续断皂苷Ⅵ在0.104~0.936mg/mL范围内呈良好的线性关系,r=0.999 6,平均加样回收率为100.17%,RSD为1.87%。结论该方法快速、灵敏,准确度高,可用于该制剂的质量控制。 展开更多
关键词 损伤复元颗粒 高效液相色谱法 川续断皂苷
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辽东楤木叶有效部位中楤木皂苷Ⅴ、Ⅵ及总皂苷质量控制 被引量:3
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作者 王秋红 王琦 +3 位作者 王知斌 杨炳友 肖洪彬 匡海学 《中成药》 CAS CSCD 北大核心 2013年第9期1950-1953,共4页
目的建立HPLC-ELSD法测定辽东楤木叶中楤木皂苷Ⅴ和Ⅵ量及可见分光光度法测定辽东楤木叶总皂苷量的方法。方法采用HPLC-ELSD法测定楤木皂苷Ⅴ和Ⅵ,Diamonsil-C18色谱柱(250 mm×4.6 mm,5μm),流动相为水-乙腈,体积流量0.7 mL/min,柱... 目的建立HPLC-ELSD法测定辽东楤木叶中楤木皂苷Ⅴ和Ⅵ量及可见分光光度法测定辽东楤木叶总皂苷量的方法。方法采用HPLC-ELSD法测定楤木皂苷Ⅴ和Ⅵ,Diamonsil-C18色谱柱(250 mm×4.6 mm,5μm),流动相为水-乙腈,体积流量0.7 mL/min,柱温35℃,漂移管温度115℃,载气体积流量3.2 L/min。采用可见分光光度法测定辽东楤木叶总皂苷量,检测波长550 nm。结果楤木皂苷Ⅴ、Ⅵ、辽东楤木叶总皂苷分别在1.253~8.768μg(r=0.999 7),1.251~8.767μg(r=0.999 7),0.1549~1.549 mg(r=0.999 8)呈良好的线性关系;加样回收率分别为99.67%(RSD为1.30%),99.17%(RSD为0.97%),99.57%(RSD为0.53%)。结论本实验采用的方法简便快速、准确、重复性好,可用于辽东楤木叶有效部位中皂苷类成分的质量控制。 展开更多
关键词 辽东楤木叶 楤木皂苷Ⅴ 楤木皂苷 HPLC-ELSD 可见分光光度法
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不同灭菌法对续断中川续断皂苷Ⅵ含量的影响 被引量:5
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作者 谭伯森 李惠琴 黄凤婷 《现代医院》 2012年第4期72-73,共2页
目的考察在保证灭菌效率的前提下,不同灭菌法对续断中川续断皂苷Ⅵ含量的影响。方法分别采用不同方法灭菌后采用高效液相色谱法测定川续断皂苷Ⅵ灭菌前后的含量。结果水洗灭菌后川续断皂苷Ⅵ含量下降最多,为8.2%,而化学灭菌为4.6%、热... 目的考察在保证灭菌效率的前提下,不同灭菌法对续断中川续断皂苷Ⅵ含量的影响。方法分别采用不同方法灭菌后采用高效液相色谱法测定川续断皂苷Ⅵ灭菌前后的含量。结果水洗灭菌后川续断皂苷Ⅵ含量下降最多,为8.2%,而化学灭菌为4.6%、热灭菌为3.8%、辐射灭菌为2.1%。结论辐照灭菌最适合续断灭菌。 展开更多
关键词 灭菌 高效液相色法 续断 川续断皂苷
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重楼皂苷Ⅶ通过p38丝裂原活化蛋白激酶信号通路对肝癌细胞恶性生物学行为的影响
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作者 朱征全 王松 +1 位作者 郭宏志 陈海洋 《安徽医药》 CAS 2024年第3期470-474,共5页
目的 观察重楼皂苷Ⅶ对肝癌细胞恶性生物学行为的影响,并探讨相关机制。方法 于2021年6月至2022年6月,取对数期人肝癌HepG2细胞,分为对照组(常规培养)、重楼皂苷Ⅶ组(重楼皂苷Ⅶ0.8μmol/L)、SB203580[p38丝裂原活化蛋白激酶(p38 MAPK)... 目的 观察重楼皂苷Ⅶ对肝癌细胞恶性生物学行为的影响,并探讨相关机制。方法 于2021年6月至2022年6月,取对数期人肝癌HepG2细胞,分为对照组(常规培养)、重楼皂苷Ⅶ组(重楼皂苷Ⅶ0.8μmol/L)、SB203580[p38丝裂原活化蛋白激酶(p38 MAPK)信号通路抑制剂]组(SB203580 10μmol/L)、联合组(重楼皂苷Ⅶ0.8μmol/L、SB203580 10μmol/L)。噻唑蓝法检测细胞增殖能力;膜联蛋白Ⅴ(AnnexinⅤ)/碘化丙啶(PI)双染法检测细胞凋亡率;划痕实验检测细胞迁移能力;小室实验检测细胞侵袭能力;蛋白质印迹法检测细胞p38 MAPK、磷酸化p38丝裂原活化蛋白激酶(p-p38 MAPK)、细胞外信号调节激酶(ERK)1/2、磷酸化细胞外信号调节激酶(p-ERK1/2)蛋白表达。结果 与对照组24、48、72 h吸光度值,迁移率(74.33±9.37)%,凋亡率(3.25±0.78)%,p-p38 MAPK/p38 MAPK、p-ERK1/2/ERK1/2及侵袭细胞数(364.92±47.99)个比较,重楼皂苷Ⅶ组24、48、72 h吸光度值,迁移率(11.21±3.35)%降低,凋亡率(39.87±8.94)%,p-p38 MAPK/p38 MAPK、p-ERK1/2/ERK1/2升高,侵袭细胞数(54.84±7.41)个减少(P<0.05);SB203580组24、48、72 h吸光度值,迁移率(89.30±14.56)%升高,凋亡率(1.05±0.15)%,p-p38MAPK/p38 MAPK、p-ERK1/2/ERK1/2降低,侵袭细胞数(617.04±75.34)个增加(P<0.05)。与重楼皂苷Ⅶ组比较,联合组24、48、72 h吸光度值,迁移率(40.52±8.18)%升高,凋亡率(11.30±2.80)%,p-p38 MAPK/p38 MAPK、p-ERK1/2/ERK1/2降低,侵袭细胞数(141.36±16.75)个增加(P<0.05);与SB203580组比较,联合组24、48、72 h吸光度值、迁移率降低,凋亡率、p-p38 MAPK/p38MAPK、p-ERK1/2/ERK1/2升高,侵袭细胞数减少(P<0.05)。结论 重楼皂苷Ⅶ可抑制肝癌HepG2细胞增殖、迁移及侵袭等恶性生物学行为,并诱导其凋亡,作用机制可能与激活p38 MAPK信号通路相关。 展开更多
关键词 重楼 皂苷类 P38丝裂原活化蛋白激酶 肝癌 增殖 侵袭 迁移
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HPLC测定妇科止血灵片中川续断皂苷Ⅵ的含量 被引量:1
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作者 贾树娟 黄雪莹 《中国医药导报》 CAS 2010年第10期80-81,共2页
目的:建立高效液相法测定妇科止血灵片中川续断皂苷Ⅵ含量的方法。方法:采用Diamonsil-C18(4.6mm×250mm,5μm)色谱柱;流动相:乙腈-0.05%磷酸(28∶72);检测波长:212nm;流速:1.0ml/min。结果:川续断皂苷Ⅵ在0.0012~0.0110μg范围内... 目的:建立高效液相法测定妇科止血灵片中川续断皂苷Ⅵ含量的方法。方法:采用Diamonsil-C18(4.6mm×250mm,5μm)色谱柱;流动相:乙腈-0.05%磷酸(28∶72);检测波长:212nm;流速:1.0ml/min。结果:川续断皂苷Ⅵ在0.0012~0.0110μg范围内与峰面积呈良好的线性关系(r=0.9992)。平均回收率为99.58%(RSD=0.94%)。结论:本方法简便、准确、重现性好,可用于妇科止血灵片中川续断皂苷Ⅵ的质量控制。 展开更多
关键词 高效液相法 续断皂苷 妇科止血灵片 含量测定
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Unraveling the serial glycosylation in the biosynthesis of steroidal saponins in the medicinal plant Paris polyphylla and their antifungal action 被引量:1
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作者 Yuegui Chen Qin Yan +9 位作者 Yunheng Ji Xue Bai Desen Li Rongfang Mu Kai Guo Minjie Yang Yang Tao Jonathan Gershenzon Yan Liu Shenghong Li 《Acta Pharmaceutica Sinica B》 SCIE CAS CSCD 2023年第11期4638-4654,共17页
Sugar-sugar glycosyltransferases play important roles in constructing complex and bioactive saponins.Here,we characterized a series of UDP-glycosyltransferases responsible for biosynthesizing the branched sugar chain ... Sugar-sugar glycosyltransferases play important roles in constructing complex and bioactive saponins.Here,we characterized a series of UDP-glycosyltransferases responsible for biosynthesizing the branched sugar chain of bioactive steroidal saponins from a widely known medicinal plant Paris polyphylla var.yunnanensis.Among them,a 2'-O-rhamnosyltransferase and three 6'-O-glucosyltrasferases catalyzed a cascade of glycosylation to produce steroidal diglycosides and triglycosides,respectively.These UDP-glycosyltransferases showed astonishing substrate promiscuity,resulting in the generation of a panel of 24 terpenoid glycosides including 15 previously undescribed compounds.A mutant library containing 44 variants was constructed based on the identification of critical residues by molecular docking simulations and protein model alignments,and a mutant UGT91AH1^(Y187A)with increased catalytic efficiency was obtained.The steroidal saponins exhibited remarkable antifungal activity against four widespread strains of human pathogenic fungi attributed to ergosterol-dependent damage of fungal cell membranes,and 2'-O-rhamnosylation appeared to correlate with strong antifungal effects.The findings elucidated the biosynthetic machinery for their production of steroidal saponins and revealed their potential as new antifungal agents. 展开更多
关键词 paris polyphylla var.yunnanensis Steroidal saponins GLYCOSYLTRANSFERASES Sugar chain elongation Antifungal activity
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云南重楼的皂苷类化学成分研究
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作者 陈文文 郑淑娴 +5 位作者 胡晋铭 田韵远 曹宇 陆云阳 刘杨 汤海峰 《中南药学》 CAS 2024年第4期851-856,共6页
目的 研究云南重楼根状茎中的皂苷类化学成分。方法 利用硅胶柱色谱、硅胶C-18柱色谱、Sephadex LH-20凝胶柱色谱以及半制备高效液相色谱等技术,从云南重楼根茎70%乙醇提取物的水饱和正丁醇萃取物中,纯化得到单体成分。通过NMR、MS等波... 目的 研究云南重楼根状茎中的皂苷类化学成分。方法 利用硅胶柱色谱、硅胶C-18柱色谱、Sephadex LH-20凝胶柱色谱以及半制备高效液相色谱等技术,从云南重楼根茎70%乙醇提取物的水饱和正丁醇萃取物中,纯化得到单体成分。通过NMR、MS等波谱分析和酸水解等化学方法对其进行结构鉴定。结果 共分离得到6个皂苷类化合物,分别鉴定为parisyunnanosideⅠ(1)、paristenoside B(2)、26-O-β-D-吡喃葡萄糖基-3β,16α,26-三羟基-(23R,25R)-16,23-环胆甾烷醇-5,17(20)-二烯-22-酮-3-O-α-L-呋喃阿拉伯糖基-(1→4)-[α-L-吡喃鼠李糖基-(1→2)]-β-D-吡喃葡萄糖苷(3)、26-O-β-D-吡喃葡萄糖基-17,22-二羟基-(25R)-呋甾-5-烯-3-O-α-L-吡喃鼠李糖基-(1→2)-β-D-吡喃葡萄糖苷(4)、parisyunnanoside A(5)和26-O-β-D-吡喃葡萄糖基-3β,26-二羟基-(25R)-胆甾-5,17(20)-二烯-16,22-二酮-3-O-α-L-吡喃鼠李糖基-(1→4)-[α-L-吡喃鼠李糖基-(1→2)]-β-D-吡喃葡萄糖苷(6)。结论 化合物3、4和6为首次从云南重楼中发现。 展开更多
关键词 云南重楼 皂苷 化学成分
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云南重楼果皮中1个新的C_(21)甾体皂苷
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作者 苏会梅 聂伟 +4 位作者 赵邦 李倩 冯莉萍 邓亚 万近福 《中药材》 CAS 北大核心 2024年第1期85-90,共6页
目的:研究云南重楼果皮中的甾体皂苷类成分。方法:采用HPD-100大孔吸附树脂柱、正相硅胶柱、ODS柱、Sephadex LH-20葡聚糖凝胶柱和半制备型高效液相色谱等方法对云南重楼果皮70%乙醇渗漉提取物中的甾体皂苷类成分进行系统研究,通过现代... 目的:研究云南重楼果皮中的甾体皂苷类成分。方法:采用HPD-100大孔吸附树脂柱、正相硅胶柱、ODS柱、Sephadex LH-20葡聚糖凝胶柱和半制备型高效液相色谱等方法对云南重楼果皮70%乙醇渗漉提取物中的甾体皂苷类成分进行系统研究,通过现代波谱学技术结合参考文献对化合物结构进行鉴定。结果:从云南重楼果皮中分离得到8个甾体皂苷类化合物,分别鉴定为:17α-hydroperoxide pregn-5-en-20-one-3-O-α-L-rhamnopyranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranoside(1)、重楼皂苷Ⅳ(2)、ypsilandroside L(3)、重楼皂苷Ⅲ(4)、pregna-5,16-dien-3β-ol-20-one-3β-O-α-L-rhamnopyranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranoside(5)、pregna-5,16-dien-3β-ol-20-one-3β-O-α-L-rhamnopyranosyl-(1→2)-[α-L-arabinopyranosyl-(1→4)]-β-D-glucopyranoside(6)、pregna-5,16-dien-3β-ol-20-one-3β-O-α-L-rhamnopyranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranoside(7)、aethioside A(8)。结论:其中,化合物1为一个新的C_(21)甾体皂苷类化合物,化合物3、5、6、8为首次从重楼属植物中分离得到。 展开更多
关键词 云南重楼果皮 C_(21)甾体皂苷 化学成分 结构鉴定
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