Application of 16s rDNA Sequencing in the Analysis of Pathogenic Bacteria in Sputum of Severe Bacterial Pneumonia

Objective: 120 patients with severe pneumonia who were kept in the comprehensive ICU of our hospital in 2018 were selected, and 16s rDNA sequencing was performed to analyze the composition of pathogenic bacteria in the sputum of severe pneumonia. Methods: The sputum samples of patients with severe bacterial pneumonia were collected, and the diversity of pathogens in the samples was analyzed by polymerase chain reaction (PCR) amplification and high-throughput sequencing (16s rDNA PCR-DGGE). Results: Sequence showed that sputum samples contained a relatively large number of species, and there were many species that were not detected by sequencing. The dominant bacteria were Streptococcus, Sphingomonas, Corynebacterium, Denatobacteria, Aquobacteria, Acinetobacteria, Prevotella, Klebsiella, Pseudomonas, etc. Conclusion: Bacteria caused by sputum of severe bacterial pneumonia are complex and diverse, which provides new methods and ideas for individualized treatment of patients with severe pneumonia.

Application of 16S rDNA Sequencing Technology in the Analysis of Pathogenic Bacteria in Sputum of Severe Pneumonia

The diagnosis of pathogenic bacteria in severe pneumonia is difficult and the prognosis is poor. Its outcome is closely related to bacterial pathogenicity and the timeliness and pertinence of antibiotic treatment. Therefore, early diagnosis is of great significance to the prognosis of patients. Sputum examination and culture is the gold standard for the diagnosis of pathogens of severe pneumonia. However, due to the long time of bacterial culture, the early use of antibiotics, the change of bacteria species, mixed infection and other problems, the results of bacterial culture in sputum are often false negative. With the continuous application of new molecular biology techniques in clinical detection, the classification of bacteria and microorganisms has deepened from the identification of phenotypic characteristics to the classification of gene characteristics. Sequencing analysis with 16S rDNA sequencing technology has the characteristics of high sequencing flux, large amount of data obtained, short cycle, and can more comprehensively reflect the species composition of microbial community, real species distribution and abundance information. In this paper, 16S rDNA sequencing technology was used to analyze the bacterial population composition in the sputum of severe pneumonia, and to explore a new method of etiological diagnosis.

Evaluation of antibacterial potential of seaweeds occurring along the coast of Mandapam,India against human pathogenic bacteria

Objective:To investigate the antibacterial activity of six seaweeds against twelve human bacterial pathogens using seven different solvents.Methods:Antibacterial activity was evaluated using the disc diffusion technique on Mueller Hinton agar.Broth dilution assays are standard method used to compare the inhibition efficiency of the antimicrobial agents.Results:Among the six seaweeds tested,the antibacterial activity was higher in the brown seaweeds when compared to red and green seaweeds.Padina gymnospora was found exerting a strong antibacterial activity with a high range inhibition[(6.00±0.82)to(22.67±1.25)mm].Broth dilution assay was conducted against Salmonella sp.with the chloroform extract of Padina gymnospora and the growth inhibition was found in the range of 74.26%to 82.56%.Conclusions:Further research studies are being carried out on other species of seaweeds of different habitats in order to provide complete data of the antimicrobial potential of these algae.It is also essential to study the principle compound present in the seaweeds which is responsible for antimicrobial activity.It can be achieved by using advanced separation techniques.