The Relationship Between Gene Mutation and Pathological Type of Lung Cancer

Objective: To investigate the relationship between gene mutation and pathological type of lung cancer, inspect and verify the consistency between homologous genes mutation in various pathologic type. Methods: Combined with the COSMIC and Uni Prot database, we obtained the reported overall big-sample mutation data of lung cancer and the protein sequences of the top 20 mutated genes,respectively. Analyze the data and cluster the protein sequences and then deduce the homologous gene. Ultimately, analyze the mutations of different pathological types of homologous genes. Results: TP53(32.32%) has the highest mutation rate in lung cancer, followed by EGFR(29.12%). The copy number variability(CNV) of genes:KRAS, LRP1 B, CDKN2 A, KMT2 C, FAT1, PIK3 CA, RB1, ERBB4, GRIN2 A and KDR between each pathological type is statically significant(P<0.05). The gene differential expression rate between adenocarcinoma and squamous carcinoma of gene TP53, KRAS, LRP1 B, CDKN2 A, STK11, FAT4, KMT2 D, NFE2 L2, KEAP1,PIK3 CA, RB1, ERBB4, SMARCA4 and KDR are statistically significant(P<0.05).The similarity of the protein sequence of EGFR and ERBB4 can reach 93%, and FAT4 and FAT1 are 81%. For small cell carcinoma, there's no difference in CNV between the two groups of homologous genes, and no difference between FAT4 and FAT1 in adenocarcinoma. Conclusion: The CNV and gene expression of lung cancer-associated genes are relevant to pathologic types. GFR and ERBB4 are homologous, FAT4 and FAT1 are also among the top 20 mutation genes.Additionally, there's no difference in CNV between the two groups of small cell carcinoma, which is the same between FAT4 and FAT1 in adenocarcinoma.

Predictive factors and model validation of post-colon polyp surgery Helicobacter pylori infection

BACKGROUND Recently,research has linked Helicobacter pylori(H.pylori)stomach infection to colonic inflammation,mediated by toxin production,potentially impacting colorectal cancer occurrence.AIM To investigate the risk factors for post-colon polyp surgery,H.pylori infection,and its correlation with pathologic type.METHODS Eighty patients who underwent colon polypectomy in our hospital between January 2019 and January 2023 were retrospectively chosen.They were then randomly split into modeling(n=56)and model validation(n=24)sets using R.The modeling cohort was divided into an H.pylori-infected group(n=37)and an H.pylori-uninfected group(n=19).Binary logistic regression analysis was used to analyze the factors influencing the occurrence of H.pylori infection after colon polyp surgery.A roadmap prediction model was established and validated.Finally,the correlation between the different pathological types of colon polyps and the occurrence of H.pylori infection was analyzed after colon polyp surgery.RESULTS Univariate results showed that age,body mass index(BMI),literacy,alcohol consumption,polyp pathology type,high-risk adenomas,and heavy diet were all influential factors in the development of H.pylori infection after intestinal polypectomy.Binary multifactorial logistic regression analysis showed that age,BMI,and type of polyp pathology were independent predictors of the occurrence of H.pylori infection after intestinal polypectomy.The area under the receiver operating characteristic curve was 0.969[95%confidence interval(95%CI):0.928–1.000]and 0.898(95%CI:0.773–1.000)in the modeling and validation sets,respectively.The slope of the calibration curve of the graph was close to 1,and the goodness-of-fit test was P>0.05 in the two sets.The decision analysis curve showed a high rate of return in both sets.The results of the correlation analysis between different pathological types and the occurrence of H.pylori infection after colon polyp surgery showed that hyperplastic polyps,inflammatory polyps,and the occurrence of H.pylori infection were not significantly correlated.In contrast,adenomatous polyps showed a significant positive correlation with the occurrence of H.pylori infection.CONCLUSION Age,BMI,and polyps of the adenomatous type were independent predictors of H.pylori infection after intestinal polypectomy.Moreover,the further constructed column-line graph prediction model of H.pylori infection after intestinal polypectomy showed good predictive ability.

Primary lacrimal sac lymphoma:a case-based retrospective study in a Chinese population

AIM:To determine the clinical characteristics,pathological types,tumor markers,treatments,and outcomes of Chinese patients with primary lacrimal sac lymphoma.METHODS:This case-based retrospective study analyzed 15 Chinese patients with primary lacrimal sac lymphoma.The clinical data collected included gender,age at diagnosis,symptoms,imaging examination results,pathologic diagnosis,pathogen identification,tumor markers,treatments,follow-up,and prognosis.Descriptive statistics were used to characterize the patients.Progression-free survival(PFS)was defined as the time from surgery to the last follow-up,first record of tumor recurrence,or death.RESULTS:There were 7 males and 8 females with unilateral primar y lacrimal sac lymphoma in the lef t eye(n=6)or right eye(n=9).The initial symptom in 13 patients was epiphora,and 2 patients had redness and swelling in the lacrimal sac area.All patients ultimately developed epiphora,and 12 had masses in the lacrimal sac area.Analysis of preoperative plasma tumor markers indicated 14 patients had elevated homocysteine,9 had elevatedβ2-microglobulin,and 2 had elevated lactate dehydrogenase(LDH);2 patients had elevations of all three markers,and 1 patient had no elevation of any marker.All patients underwent surgical resection and 12 patients received postoperative chemotherapy.The pathological types were DLBCL(n=8),MALT lymphoma(n=5),and NK/T-cell lymphoma,nasal type(n=2).The mean followup time was 25.8mo(range:4-41)and 2 patients died.Seven patients who underwent mass excision combined with dacryocystorhinostomy(DCR)had no postoperative epiphora.Eight patients who only underwent mass excision had varying degrees of postoperative epiphora.Preoperative LDH elevation and NK/T-cell lymphoma,nasal type were associated with poor prognoses.CONCLUSION:Early diagnosis and treatment can lead to a good prognosis for most patients with primary lacrimal sac lymphoma.Mass resection combined with DCR can reduce the occurrence of post-surgical epiphora.The pathology type and tumor marker status are associated with prognosis.

Impact of smoking status and pathologic type on epidermal growth factor receptor mutations in lung cancer

Background Epidermal growth factor receptor （EGFR） responsive to the treatment with tyrosine kinase inhibitors. large series of lung carcinomas. mutations in lung carcinomas can make the disease more We aimed to evaluate the prevalence of EGFR mutations in a Methods We examined 1195 consecutive lung cancer patients for EGFR mutations in exons 18, 19, and 21 using direct sequencing of polymerase chain reaction products. A detailed smoking history was obtained. Patients were categorized as never smokers （〈100 lifetime cigarettes）, former smokers （quit 〉1 year ago）, or current smokers （quit 〈1 year ago）. Results There were EGFR mutations in 9 （4.5%） of 201 squamous carcinomas, in 1 （2%） of 50 large cell carcinomas, and in 1 （2.3%） of 44 small cell carcinomas that were investigated. Three hundred and twenty-seven mutations were found in the series of 858 adenocarcinomas （38.1%）. Among 858 lung adenocarcinomas, we detected EGFR mutations in 250 （48.6%） of 514 never smokers, 39 （33.9%） of 115 former smokers, and 38 （16.6%） of 229 current smokers. Significantly fewer EGFR mutations were found in people who smoked for more than 15 pack-years （P=-0.0002） or stopped smoking less than 15 years ago （P=0.033） compared with individuals who never smoked. Conclusions Adenocarcinoma is the most frequent EGFR mutation pathologic type in lung cancer. The likelihood of EGFR mutations in exons 18, 19 and 21 decreases as the number of pack-years increases. Mutations were less common in people who smoked for more than 15 pack-years or who stopped smoking cigarettes less than 15 years ago. These data can assist clinicians in assessing the likelihood of exons 18, 19, or 21 EGFR mutations in Chinese patients with lung cancer when mutational analysis is not feasible.

The transcription factor RelB restrains group 2 innate lymphoid cells and type 2 immune pathology in vivo

The exact relationships between group 2 innate lymphoid cells(ILC2s)and Th2 cells in type 2 pathology,as well as the mechanisms that restrain the responses of these cells,remain poorly defined.Here we examined the roles of ILC2s and Th2 cells in type 2 lung pathology in vivo using germline and conditional fie/b-deficient mice.We found that mice with germline deletion of Relb^(-/-)spontaneously developed prominent type 2 pathology in the lung,which contrasted sharply with mice with T-cell-specific Relb deletion(Relb^(f/f)Cd4-Cre),which were healthy with no observed autoimmune pathology.We also found that in contrast to wild-type B6 mice,Rel6-defident mice showed markedly expanded ILC2s but not ILC1s or ILC3s.Moreover,adoptive transfer of naive CD4^(+)T cells into Rag1^(-/-)Relb^(-/-)hosts induced prominent type 2 lung pathology,which was inhibited by depletion of ILC2s.Mechanistically,we showed that Relb deletion led to enhanced expression of Bcl11b,a key transcription factor for ILC2s.We concluded that RelB plays a critical role in restraining ILC2s,primarily by suppressing Bcl11b activity,and consequently inhibits type 2 lung pathology in vivo.