The pathogen,pathogenic mechanism and incidence condition of pear ring rot,and its damage on fruits,branches and leaves are summarized in the paper. The control measures including plant quarantine,agricultural control...The pathogen,pathogenic mechanism and incidence condition of pear ring rot,and its damage on fruits,branches and leaves are summarized in the paper. The control measures including plant quarantine,agricultural control,physical control,biological control,and chemical control against pear ring rot are reviewed.展开更多
Considering the heavy losses caused by pear ring rot(Botryosphaeria berengeriana) disease, the potential biocontrol agent against B. berengeriana was isolated and characterized in this work. Bacteria, isolated from pe...Considering the heavy losses caused by pear ring rot(Botryosphaeria berengeriana) disease, the potential biocontrol agent against B. berengeriana was isolated and characterized in this work. Bacteria, isolated from pear orchard rhizosphere soil, were screened for the biological control of pear ring rot caused by B. berengeriana. Among them, strain L-1 showed noticeable inhibitory activities against B. berengeriana and six other commonly occurring postharvest pathogens. Molecular methods indicated strain L-1 was Bacillus amyloliquefaciens. The potential of strain L-1 as an effective biocontrol agent was further estimated. Results showed strain L-1 could successfully colonize in pear wounds, its colonies reached 142.35 folds on 4 days post inoculation, and maintained at a high level during storage. In addition, strain L-1 caused abnormal hyphae growth of B. berengeriana,and its inhibitory percentage against pear ring rot reached 76.55% in vivo on 11 days post inoculation. Strain L-1 significantly induced the peroxidase(POD) and catalase(CAT) activities and delayed the accumulation of malondialdehyde(MDA) in pears. What's more, strain L-1 did not impair the fruit quality. All these results suggest that B. berengeriana L-1 is a promising agent for the biocontrol of pear ring rot.展开更多
【目的】从鸭梨果实中克隆Pb Chi IV的全长c DNA序列,检测Pb Chi IV在根、茎、叶、果实以及在水杨酸(SA)和梨轮纹病菌诱导下的表达特性,以探讨该基因与SA信号转导及抗梨轮纹病菌的相关性。【方法】设计特异引物,克隆Pb Chi IV的全长序列...【目的】从鸭梨果实中克隆Pb Chi IV的全长c DNA序列,检测Pb Chi IV在根、茎、叶、果实以及在水杨酸(SA)和梨轮纹病菌诱导下的表达特性,以探讨该基因与SA信号转导及抗梨轮纹病菌的相关性。【方法】设计特异引物,克隆Pb Chi IV的全长序列,将测序得到的核苷酸序列和推导的氨基酸序列在NCBI上用BLAST进行序列相似性分析,利用Biot Edit软件对氨基酸序列进行比对,利用MEGA6.0构建系统发育树,利用实时荧光定量PCR技术分析该基因在梨不同组织以及在SA和梨轮纹病菌诱导下的表达。【结果】克隆了Pb Chi IV的c DNA序列为819 bp,Gen Bank数据库登录号为KJ872676。生物信息学分析表明,Pb Chi IV编码272个氨基酸,与沙梨的同源性达100%,与毛果杨(XP_006376418.1)、葡萄(NP001268173.1)、拟南芥(CAA74930.1)、紫花苜蓿(ACL36992.1)、蒺藜苜蓿(AAR87869.1)、豇豆(CAA61281.1)、榛子(AEM97876.1)、东方山羊豆(AAP03085.1)、葡萄(NP_001268075.1)、华东葡萄(ABY66958.1)、葡萄(AAB65777.1)、烟草(BAF44533.1)和海岛棉(AER29902.1)的同源性分别为79%、73%、73%、72%、72%、72%、69%、68%、67%、67%、65%、67%和62%,属于第IV类几丁质酶基因。表达分析表明,Pb Chi IV在根中的表达量最大,分别是茎和叶的4.32和2.96倍,其次是在果实中的表达量,分别是茎和叶的2.48和1.70倍,在叶片和茎中的表达相对较低。在鸭梨幼果和成熟期果实中,SA和梨轮纹病菌均可诱导该基因表达。SA处理后基因的最大表达量是对照的2.83和3.8倍,病原菌处理后基因的最大表达量是对照的1.82和1.66倍,SA、病原菌处理后基因的最大表达量是对照的2.49和3.43倍,表达量分别在72、24和72 h达到最大值。【结论】Pb Chi IV可能参与SA介导的植物抗病防卫反应的信号通路,推测其参与梨轮纹病菌引起的防卫反应,在鸭梨抗病过程中起作用。展开更多
文摘The pathogen,pathogenic mechanism and incidence condition of pear ring rot,and its damage on fruits,branches and leaves are summarized in the paper. The control measures including plant quarantine,agricultural control,physical control,biological control,and chemical control against pear ring rot are reviewed.
基金funded by the National Key Research and Development Program (2016YFD0400903-06)China Agriculture Research System (CARS-29-19)Chinese Academy of Agriculture Association, Agriculture Science and Technology Innovation Project (CAAS-ASTIP-2015-RIP-06)
文摘Considering the heavy losses caused by pear ring rot(Botryosphaeria berengeriana) disease, the potential biocontrol agent against B. berengeriana was isolated and characterized in this work. Bacteria, isolated from pear orchard rhizosphere soil, were screened for the biological control of pear ring rot caused by B. berengeriana. Among them, strain L-1 showed noticeable inhibitory activities against B. berengeriana and six other commonly occurring postharvest pathogens. Molecular methods indicated strain L-1 was Bacillus amyloliquefaciens. The potential of strain L-1 as an effective biocontrol agent was further estimated. Results showed strain L-1 could successfully colonize in pear wounds, its colonies reached 142.35 folds on 4 days post inoculation, and maintained at a high level during storage. In addition, strain L-1 caused abnormal hyphae growth of B. berengeriana,and its inhibitory percentage against pear ring rot reached 76.55% in vivo on 11 days post inoculation. Strain L-1 significantly induced the peroxidase(POD) and catalase(CAT) activities and delayed the accumulation of malondialdehyde(MDA) in pears. What's more, strain L-1 did not impair the fruit quality. All these results suggest that B. berengeriana L-1 is a promising agent for the biocontrol of pear ring rot.
文摘【目的】从鸭梨果实中克隆Pb Chi IV的全长c DNA序列,检测Pb Chi IV在根、茎、叶、果实以及在水杨酸(SA)和梨轮纹病菌诱导下的表达特性,以探讨该基因与SA信号转导及抗梨轮纹病菌的相关性。【方法】设计特异引物,克隆Pb Chi IV的全长序列,将测序得到的核苷酸序列和推导的氨基酸序列在NCBI上用BLAST进行序列相似性分析,利用Biot Edit软件对氨基酸序列进行比对,利用MEGA6.0构建系统发育树,利用实时荧光定量PCR技术分析该基因在梨不同组织以及在SA和梨轮纹病菌诱导下的表达。【结果】克隆了Pb Chi IV的c DNA序列为819 bp,Gen Bank数据库登录号为KJ872676。生物信息学分析表明,Pb Chi IV编码272个氨基酸,与沙梨的同源性达100%,与毛果杨(XP_006376418.1)、葡萄(NP001268173.1)、拟南芥(CAA74930.1)、紫花苜蓿(ACL36992.1)、蒺藜苜蓿(AAR87869.1)、豇豆(CAA61281.1)、榛子(AEM97876.1)、东方山羊豆(AAP03085.1)、葡萄(NP_001268075.1)、华东葡萄(ABY66958.1)、葡萄(AAB65777.1)、烟草(BAF44533.1)和海岛棉(AER29902.1)的同源性分别为79%、73%、73%、72%、72%、72%、69%、68%、67%、67%、65%、67%和62%,属于第IV类几丁质酶基因。表达分析表明,Pb Chi IV在根中的表达量最大,分别是茎和叶的4.32和2.96倍,其次是在果实中的表达量,分别是茎和叶的2.48和1.70倍,在叶片和茎中的表达相对较低。在鸭梨幼果和成熟期果实中,SA和梨轮纹病菌均可诱导该基因表达。SA处理后基因的最大表达量是对照的2.83和3.8倍,病原菌处理后基因的最大表达量是对照的1.82和1.66倍,SA、病原菌处理后基因的最大表达量是对照的2.49和3.43倍,表达量分别在72、24和72 h达到最大值。【结论】Pb Chi IV可能参与SA介导的植物抗病防卫反应的信号通路,推测其参与梨轮纹病菌引起的防卫反应,在鸭梨抗病过程中起作用。
