采用均匀沉淀法合成Zn O纳米颗粒(Zn O NPs),以Zn O NPs为种子,制备水溶性Au/Zn O异质结构。将Au/Zn O异质结构附着于离子液体功能化石墨烯(GN)复合膜上,形成一种新颖的负载型石墨烯复合材料(Au/Zn O/GN)。所构建的青霉素酶-氧化苏木精...采用均匀沉淀法合成Zn O纳米颗粒(Zn O NPs),以Zn O NPs为种子,制备水溶性Au/Zn O异质结构。将Au/Zn O异质结构附着于离子液体功能化石墨烯(GN)复合膜上,形成一种新颖的负载型石墨烯复合材料(Au/Zn O/GN)。所构建的青霉素酶-氧化苏木精修饰Au/Zn O/GN(PH-AZG)传感器在PBS水溶液(p H=7.0)中对青霉素钠检测线性范围为2.5×10^(-14)~3.3×10^(-6)mol/L,检出限达到1.5×10^(-14)mol/L(S/N≥3)。在相同条件下制备5根PH-AZG电极,其响应电流的相对标准偏差(RSD)小于3.2%。同时,在实际牛奶制品中,本方法的检测线性范围为5×10^(-14)~5×10^(-7)mol/L,加标回收率为99.7%~101.4%,RSD为2.3%~3.5%(n=5)。结果表明,本方法对实际牛奶制品中低浓度青霉素钠的检测具有可行性。展开更多
将玻碳电极进行阳极氧化和氨基化修饰,通过碳二亚胺盐酸盐（EDC）、N-羟基丁二酰亚胺（NHS）活化作用将青霉素适配子结合在电极表面。该适配子电化学生物传感器分子识别能力强、无放射性标记、检测速率快,青霉素类的最佳检测范围是2.81~...将玻碳电极进行阳极氧化和氨基化修饰,通过碳二亚胺盐酸盐（EDC）、N-羟基丁二酰亚胺（NHS）活化作用将青霉素适配子结合在电极表面。该适配子电化学生物传感器分子识别能力强、无放射性标记、检测速率快,青霉素类的最佳检测范围是2.81~281 nmol/L,最低检测限为2.81 nmol/L,检测时间为5 m in。展开更多
Penicillinase was immobilized by glutaraldehyde-bovine serum albumin and sodium alginate was used for the construction of enzyme biosensors in determination of trace penicillin in milk. Penicillinase activity was dete...Penicillinase was immobilized by glutaraldehyde-bovine serum albumin and sodium alginate was used for the construction of enzyme biosensors in determination of trace penicillin in milk. Penicillinase activity was determined by potentionmetric method. At low concentrations, the response time of the electrode modified by the method of crossing-link was 30～60 s, and the electrode showed high sensitivity. Linear detection range from 1.40×10 -3 to 3.36×10 -2 mmol/L in PBS and from 5.61×10 -2 to 3.36× 10 -1 mmol/L in milk were achieved. The electrode can be used for at least 7 d with perfect reproducibility during the first 4 d. The life of the electrode was 30 d.展开更多
文摘将玻碳电极进行阳极氧化和氨基化修饰,通过碳二亚胺盐酸盐（EDC）、N-羟基丁二酰亚胺（NHS）活化作用将青霉素适配子结合在电极表面。该适配子电化学生物传感器分子识别能力强、无放射性标记、检测速率快,青霉素类的最佳检测范围是2.81~281 nmol/L,最低检测限为2.81 nmol/L,检测时间为5 m in。
文摘Penicillinase was immobilized by glutaraldehyde-bovine serum albumin and sodium alginate was used for the construction of enzyme biosensors in determination of trace penicillin in milk. Penicillinase activity was determined by potentionmetric method. At low concentrations, the response time of the electrode modified by the method of crossing-link was 30～60 s, and the electrode showed high sensitivity. Linear detection range from 1.40×10 -3 to 3.36×10 -2 mmol/L in PBS and from 5.61×10 -2 to 3.36× 10 -1 mmol/L in milk were achieved. The electrode can be used for at least 7 d with perfect reproducibility during the first 4 d. The life of the electrode was 30 d.
