Elderly patients with systemic disseminated <em>Penicillium marneffei </em>are generally more seriously ill with high mortality. In addition to clear diagnosis and timely treatment, careful nursing through...Elderly patients with systemic disseminated <em>Penicillium marneffei </em>are generally more seriously ill with high mortality. In addition to clear diagnosis and timely treatment, careful nursing throughout the hospitalization is particularly important for the patient’s recovery. Psychological nursing and family emotional support, the treatment of adverse drug reactions, systemic infection wound care, the prevention of pressure sores in long-term bedridden patients, antithrombotic nursing, disinfection isolation and the standard prevention of nosocomial cross infection all play decisive roles in the patient’s prognosis. After one month treatment and medical care, the patient was cured and was discharged from hospital. After discharge, the patient follow-up was carried out up to one year. This patient had completely recovered. In this paper, we report a successful nursing experience of an elderly patient with systemic disseminated <em>Penicillium marneffei.</em> With the summary of nursing key points, it could play a reference in the treatment and rehabilitation of similar patients.展开更多
Penicillium marneffei(P.marneffei)is a pathogenic fungus that can cause fatal infections in humans,particularly immunocompromised hosts.It is prevalent only in the Southeast Asian region.A 5-year-old boy was presented...Penicillium marneffei(P.marneffei)is a pathogenic fungus that can cause fatal infections in humans,particularly immunocompromised hosts.It is prevalent only in the Southeast Asian region.A 5-year-old boy was presented with diagnosed Acute Lymphoblastic Leukemia undergoing chemotherapy and suffering from high-grade fever for 15 days positively responded to antibiotics on the initial day,but later he became unresponsive.He was found negative for blood cultures of bacterial growth.Ultrasound of the abdomen showed a borderline enlarged liver with multiple small hypoechoic areas of variable sizes seen scattered in both lobes of the liver.Morphological and microscopic analyses of liver tissues showed multiple septate hyphae of P.marneffei.Amphotericin B was found to be effective in treating the child.Appropriate identification is essential to treat the pathogen-causing disease,especially in immuno-compromised conditions like a leukemic patient,and determine underlying mechanisms behind the pathogenesis.展开更多
Penicillium marneffei (P. marneffei ) infection usually occurs with skin, bone marrow, lung or hepatic involvement. However, no cases of P. marneffei infection with chylous ascites have been reported thus far. In this...Penicillium marneffei (P. marneffei ) infection usually occurs with skin, bone marrow, lung or hepatic involvement. However, no cases of P. marneffei infection with chylous ascites have been reported thus far. In this report, we describe the first case of acquired immune deficiency syndrome (AIDS) which has been complicated by a P. marneffei infection causing chylous ascites. We describe the details of the case, with an emphasis on treatment regimen. This patient was treated with amphotericin B for 3 mo, while receiving concomitant therapy with an efavirenz-containing antiretroviral regimen, but cultures in ascitic fluid were persistently positive for P. marneffei . The infection resolved after treatment with high-dose voriconazole (400 mg every 12 h) for 3 mo. P. marneffei should be considered in the differential diagnosis of chylous ascites in human immunodeficiency virus patients. High-dose voriconazole is an effective, well-tolerated and convenient option for the treatment of systemic infections with P. marneffei in AIDS patients on an efavirenz-containing antiretroviral regimen.展开更多
Background Penicillium mameffei （P. marneffe~） is an emerging pathogenic fungus that can cause invasive mycosis in patients with AIDS. The epidemiological features of P. marneffeiinfection in AIDS patients in Guangd...Background Penicillium mameffei （P. marneffe~） is an emerging pathogenic fungus that can cause invasive mycosis in patients with AIDS. The epidemiological features of P. marneffeiinfection in AIDS patients in Guangdong province remain unclear so far. This study aimed to investigate the genetic diversity within a population of 163 P. mameffei isolates obtained from AIDS patients and search for the dominant clinical strains in Guanqdong province.展开更多
P enicillium marneffei ( P. marneffei ) is a facultative intracellular pathogen and the only thermally dimorphic fungus. This fungal infection is commonly found in Southeast Asian, Hong Kong, south China, and especi...P enicillium marneffei ( P. marneffei ) is a facultative intracellular pathogen and the only thermally dimorphic fungus. This fungal infection is commonly found in Southeast Asian, Hong Kong, south China, and especially in acquired immunodeficiency syndrome (AIDS) patients. 1-4 We reported a patient with AIDS in whom infection due to P. marneffei was demonstrated. CASE REPORT A 34-year-old man with human immunodeficiency virus (HIV) infection (diagnosed before 6 months), living in Guangzhou of south China, was admitted to the hospital because of prolonged fever, cough, and weight loss. Initial examination revealed permanent high-grade fever (39-40℃) with chill and cough. Laboratory findings revealed haemoglobin concentration at 80 g/L, CD 4 lymphocyte count at 7×10 6/L, and viral load at 1.3×10 5 copies/ml. Blood and urine culture revealed no bacterial growth. Sputum culture was negative for mycobacterium. Chest X-ray film revealed left upper lobe ill-defined parenchymal opacities and widening of the superior mediastinum with left lung hilum prominence (Fig. 1). Abdominal B ultrasound showed hepatoplenomegaly and coeliac enlarged lymph nodes. On physical examination, the patient was found to have facial papula, cervical and superclavicular multiple lymph nodes.展开更多
目的研究马尔尼菲青霉对巨噬细胞模式识别受体TLR-2、TLR-4、Dectin-1的表达及促炎因子TNF-α分泌的影响。方法马尔尼菲青霉酵母相菌液与小鼠腹腔巨噬细胞共培养24h,采用流式细胞技术检测巨噬细胞TLR-2、TLR-4及Dectin-1的平均荧光强度...目的研究马尔尼菲青霉对巨噬细胞模式识别受体TLR-2、TLR-4、Dectin-1的表达及促炎因子TNF-α分泌的影响。方法马尔尼菲青霉酵母相菌液与小鼠腹腔巨噬细胞共培养24h,采用流式细胞技术检测巨噬细胞TLR-2、TLR-4及Dectin-1的平均荧光强度;共聚焦显微镜观察荧光染色的受体;ELISA法测定培养液上清中TNF-α的浓度;Real time PCR检测不同时间段TNF-α的mRNA表达。结果马尔尼菲青霉可使巨噬细胞TLR-2、TLR-4、Dectin-1的平均荧光强度均增高,并激活巨噬细胞产生TNF-α。结论马尔尼菲青霉上调了巨噬细胞模式识别受体TLR-2、TLR-4及Dectin-1的表达,巨噬细胞的激活与TLR-2、TLR-4及Dectin-1的表达上调相关。展开更多
文摘Elderly patients with systemic disseminated <em>Penicillium marneffei </em>are generally more seriously ill with high mortality. In addition to clear diagnosis and timely treatment, careful nursing throughout the hospitalization is particularly important for the patient’s recovery. Psychological nursing and family emotional support, the treatment of adverse drug reactions, systemic infection wound care, the prevention of pressure sores in long-term bedridden patients, antithrombotic nursing, disinfection isolation and the standard prevention of nosocomial cross infection all play decisive roles in the patient’s prognosis. After one month treatment and medical care, the patient was cured and was discharged from hospital. After discharge, the patient follow-up was carried out up to one year. This patient had completely recovered. In this paper, we report a successful nursing experience of an elderly patient with systemic disseminated <em>Penicillium marneffei.</em> With the summary of nursing key points, it could play a reference in the treatment and rehabilitation of similar patients.
文摘Penicillium marneffei(P.marneffei)is a pathogenic fungus that can cause fatal infections in humans,particularly immunocompromised hosts.It is prevalent only in the Southeast Asian region.A 5-year-old boy was presented with diagnosed Acute Lymphoblastic Leukemia undergoing chemotherapy and suffering from high-grade fever for 15 days positively responded to antibiotics on the initial day,but later he became unresponsive.He was found negative for blood cultures of bacterial growth.Ultrasound of the abdomen showed a borderline enlarged liver with multiple small hypoechoic areas of variable sizes seen scattered in both lobes of the liver.Morphological and microscopic analyses of liver tissues showed multiple septate hyphae of P.marneffei.Amphotericin B was found to be effective in treating the child.Appropriate identification is essential to treat the pathogen-causing disease,especially in immuno-compromised conditions like a leukemic patient,and determine underlying mechanisms behind the pathogenesis.
文摘Penicillium marneffei (P. marneffei ) infection usually occurs with skin, bone marrow, lung or hepatic involvement. However, no cases of P. marneffei infection with chylous ascites have been reported thus far. In this report, we describe the first case of acquired immune deficiency syndrome (AIDS) which has been complicated by a P. marneffei infection causing chylous ascites. We describe the details of the case, with an emphasis on treatment regimen. This patient was treated with amphotericin B for 3 mo, while receiving concomitant therapy with an efavirenz-containing antiretroviral regimen, but cultures in ascitic fluid were persistently positive for P. marneffei . The infection resolved after treatment with high-dose voriconazole (400 mg every 12 h) for 3 mo. P. marneffei should be considered in the differential diagnosis of chylous ascites in human immunodeficiency virus patients. High-dose voriconazole is an effective, well-tolerated and convenient option for the treatment of systemic infections with P. marneffei in AIDS patients on an efavirenz-containing antiretroviral regimen.
基金This work was supported by the grants from the Key Subject Programs of Guangzhou Municipal Health Bureau （No. 2009-Zdi-16） the Natural Science Foundation of Guangdong Province （No. 10151006002000000） and Chinese National llth Five-year Plan on Key Infectious diseases （No. 2008ZX10001-006）.
文摘Background Penicillium mameffei （P. marneffe~） is an emerging pathogenic fungus that can cause invasive mycosis in patients with AIDS. The epidemiological features of P. marneffeiinfection in AIDS patients in Guangdong province remain unclear so far. This study aimed to investigate the genetic diversity within a population of 163 P. mameffei isolates obtained from AIDS patients and search for the dominant clinical strains in Guanqdong province.
文摘P enicillium marneffei ( P. marneffei ) is a facultative intracellular pathogen and the only thermally dimorphic fungus. This fungal infection is commonly found in Southeast Asian, Hong Kong, south China, and especially in acquired immunodeficiency syndrome (AIDS) patients. 1-4 We reported a patient with AIDS in whom infection due to P. marneffei was demonstrated. CASE REPORT A 34-year-old man with human immunodeficiency virus (HIV) infection (diagnosed before 6 months), living in Guangzhou of south China, was admitted to the hospital because of prolonged fever, cough, and weight loss. Initial examination revealed permanent high-grade fever (39-40℃) with chill and cough. Laboratory findings revealed haemoglobin concentration at 80 g/L, CD 4 lymphocyte count at 7×10 6/L, and viral load at 1.3×10 5 copies/ml. Blood and urine culture revealed no bacterial growth. Sputum culture was negative for mycobacterium. Chest X-ray film revealed left upper lobe ill-defined parenchymal opacities and widening of the superior mediastinum with left lung hilum prominence (Fig. 1). Abdominal B ultrasound showed hepatoplenomegaly and coeliac enlarged lymph nodes. On physical examination, the patient was found to have facial papula, cervical and superclavicular multiple lymph nodes.
文摘目的研究马尔尼菲青霉对巨噬细胞模式识别受体TLR-2、TLR-4、Dectin-1的表达及促炎因子TNF-α分泌的影响。方法马尔尼菲青霉酵母相菌液与小鼠腹腔巨噬细胞共培养24h,采用流式细胞技术检测巨噬细胞TLR-2、TLR-4及Dectin-1的平均荧光强度;共聚焦显微镜观察荧光染色的受体;ELISA法测定培养液上清中TNF-α的浓度;Real time PCR检测不同时间段TNF-α的mRNA表达。结果马尔尼菲青霉可使巨噬细胞TLR-2、TLR-4、Dectin-1的平均荧光强度均增高,并激活巨噬细胞产生TNF-α。结论马尔尼菲青霉上调了巨噬细胞模式识别受体TLR-2、TLR-4及Dectin-1的表达,巨噬细胞的激活与TLR-2、TLR-4及Dectin-1的表达上调相关。
