The patch clamp recording technique in vivois a blind patch clamp recording methods to record the current of the spinal or cereral neurons of anaes：hesia （ or awake） animals. This technique can be used to study the...The patch clamp recording technique in vivois a blind patch clamp recording methods to record the current of the spinal or cereral neurons of anaes：hesia （ or awake） animals. This technique can be used to study the synaptic function and plasticity in central nervous system in vivoin order to understand the physiological properties of the ion channels from an integrated point of view. The advantage of this technique have already presented itself in the study of the synaptic transmission and nervous network. Nowadays, in vivo patch whole-cell recording technique in combination with other techniques is becoming a common method in the research fields.展开更多
The authors used suspension cells of Populus euphratica to isolate protoplast in the present study. Protoplasts were successfully obtained after 4 hours incubation in enzyme solution containing 1 0% cellulase “o...The authors used suspension cells of Populus euphratica to isolate protoplast in the present study. Protoplasts were successfully obtained after 4 hours incubation in enzyme solution containing 1 0% cellulase “onozuka” R\|10, 0\^01% pectolyase Y\|23,0\^15% macerozyme R\|10 and 0\^1% hemicellulase at 25℃. Outward and inward single channels in plasma membrane were observed using cell\|attached recording of patch\|clamp technique. In this study, single channel records showed that more than one species of channel were obtained. These attempts in protoplast isolation and ion channel recording offers the opportunity to characterize cellular mechanisms of salt tolerance in tree species.展开更多
目的研究β2受体对心肌梗死（MI）后2～8周心肌细胞钠钙交换电流（INCX）的调控作用。方法雄性健康Wistar大鼠28只，随机分为：正常对照组；MI后2周、4周、8周组。以结扎心脏左前降支方法制作MI模型。正常对照组进行假手术。采用经典的...目的研究β2受体对心肌梗死（MI）后2～8周心肌细胞钠钙交换电流（INCX）的调控作用。方法雄性健康Wistar大鼠28只，随机分为：正常对照组；MI后2周、4周、8周组。以结扎心脏左前降支方法制作MI模型。正常对照组进行假手术。采用经典的酶分离心肌细胞方法，应用全细胞膜片钳技术记录INCX，观察MI后INCX的变化，予以β2受体激动剂salbutamol、β受体激动剂异丙肾上腺素及不同选择性β受体阻滞剂后INCX的变化。结果MI后8周心肌细胞IM显著增高（1．07±0．21pA／pF vs 0．51±0．12pA／pF，P〈0．05）。MI后B2受体激动显著增加心肌细胞INCX（P〈0．05），对INCX的调节作用较正常心肌细胞强。MI后选择性β2受体阻滞剂ICI118，551对B受体激动引发的心肌细胞INCX升高的抑制程度增高；选择性β2受体阻滞剂阿替洛尔对上述作用的抑制程度下降；非选择性β受体阻滞剂普萘洛尔对β受体激动引发的正常和MI后心肌细胞上述离子通道电流升高均能明显抑制。结论MI后β2受体对心肌细胞INCX的调节作用增强，提示MI后β2受体在恶性心律失常发生机制中的地位可能提高。展开更多
文摘The patch clamp recording technique in vivois a blind patch clamp recording methods to record the current of the spinal or cereral neurons of anaes：hesia （ or awake） animals. This technique can be used to study the synaptic function and plasticity in central nervous system in vivoin order to understand the physiological properties of the ion channels from an integrated point of view. The advantage of this technique have already presented itself in the study of the synaptic transmission and nervous network. Nowadays, in vivo patch whole-cell recording technique in combination with other techniques is becoming a common method in the research fields.
文摘The authors used suspension cells of Populus euphratica to isolate protoplast in the present study. Protoplasts were successfully obtained after 4 hours incubation in enzyme solution containing 1 0% cellulase “onozuka” R\|10, 0\^01% pectolyase Y\|23,0\^15% macerozyme R\|10 and 0\^1% hemicellulase at 25℃. Outward and inward single channels in plasma membrane were observed using cell\|attached recording of patch\|clamp technique. In this study, single channel records showed that more than one species of channel were obtained. These attempts in protoplast isolation and ion channel recording offers the opportunity to characterize cellular mechanisms of salt tolerance in tree species.
文摘目的研究β2受体对心肌梗死（MI）后2～8周心肌细胞钠钙交换电流（INCX）的调控作用。方法雄性健康Wistar大鼠28只，随机分为：正常对照组；MI后2周、4周、8周组。以结扎心脏左前降支方法制作MI模型。正常对照组进行假手术。采用经典的酶分离心肌细胞方法，应用全细胞膜片钳技术记录INCX，观察MI后INCX的变化，予以β2受体激动剂salbutamol、β受体激动剂异丙肾上腺素及不同选择性β受体阻滞剂后INCX的变化。结果MI后8周心肌细胞IM显著增高（1．07±0．21pA／pF vs 0．51±0．12pA／pF，P〈0．05）。MI后B2受体激动显著增加心肌细胞INCX（P〈0．05），对INCX的调节作用较正常心肌细胞强。MI后选择性β2受体阻滞剂ICI118，551对B受体激动引发的心肌细胞INCX升高的抑制程度增高；选择性β2受体阻滞剂阿替洛尔对上述作用的抑制程度下降；非选择性β受体阻滞剂普萘洛尔对β受体激动引发的正常和MI后心肌细胞上述离子通道电流升高均能明显抑制。结论MI后β2受体对心肌细胞INCX的调节作用增强，提示MI后β2受体在恶性心律失常发生机制中的地位可能提高。
