Efficacy of recombinant human osteoprotegerin combined with tinidazole in the treatment of periodontitis mice and its correlation with serum RANKL and MCP-1 levels

Objective: To investigate the effect of recombinant human osteoprotegerin combined with tinidazole on mice with periodontitis and the effect on serum RANKL and MCP-1 levels. Methods: 80 SPF-cleaned mice were randomly divided into 4 groups, 20 each, model group, tinidazole group and recombinant human osteoprotegerin group were modeled by Kimura et al., and tinidazole group received tinidazole. After intragastric administration, the recombinant human osteoprotegerin group was injected with recombinant human osteoprotegerin in the periodontal pocket according to the tinidazole group. The periodontal changes of the four groups of mice were observed and recorded, and the gingival rating was performed. Epithelial tissue morphology was observed by hematoxylin-eosin (HE) staining. Serum levels of IL-4, IL-6, RANKL and MCP-1 were measured by enzyme-linked immunosorbent assay. Results:After the intervention, the model group developed severe inflammatory reactions, including redness, hemorrhage, and deep periodontal pockets. The teeth were significantly loosened. The mice in the tinidazole group and the recombinant human osteoprotegerin group recovered substantially, and the gingival rating of the recombinant human osteoprotegerin group was better than that. The tinidazole group and the model group (P<0.05). The results of HE staining showed that the model group had edema, vasodilation and a large amount of inflammatory infiltration. The epithelial structure of the mice in the tinidazole group and the recombinant human osteoprotegerin group was intact and arranged closely and orderly. After intervention, the IL-4 in the tinidazole group and the recombinant human osteoprotegerin group was significantly higher than the model group and IL-6 was significantly lower than the model group (P<0.05), and the recombinant human osteoprotegerin group IL-4 was significantly higher after the intervention. IL-6 was significantly lower in the tinidazole group than in the tinidazole group (P<0.05). After the intervention, the tinidazole group and the recombinant human osteoprotegerin group were significantly reduced, and the recombinant human osteoprotegerin group RAKNL and MCP-1 were significantly lower than the model group (P>0.05). Conclusion: Recombinant human osteoprotegerin combined with tinidazole has a better therapeutic effect on gums and teeth in mice with periodontitis, and can lower the levels of RAKNL and MCP-1 in serum, inhibit bone resorption and protect teeth.

牙周膜相关蛋白1在大鼠牙周组织及牙周膜细胞中表达的研究

目的研究牙周膜相关蛋白1（periodonta lligament-associated protein-1，PLAP-1）在正常牙周组织及牙周膜细胞中的分布和表达，为进一步研究PLAP-1在牙周组织中的作用奠定基础。方法用免疫组织化学、免疫细胞化学和反转录一聚合酶链反应（RT-PCR）技术，对5只正常成年雄性SD大鼠磨牙牙周组织中PLAP-1的组织分布和牙周膜细胞mRNA的表达进行分析。结果免疫组织化学结果表明，PLAP-1在牙周组织中强阳性表达于牙周膜，而在牙骨质、牙槽骨、牙龈中未见表达。免疫细胞化学结果显示，PLAP-1染色阳性细胞中着色定位于胞质中，胞核染色阴性。RT．PCR电泳结果显示350bp处出现一条目的条带，与PLAP-1mRNA表达的预期结果相同，表明牙周膜细胞在mRNA水平表达PLAP-1。结论在大鼠正常牙周组织中，PLAP-1在基因及蛋白水平均呈高表达且主要定位于牙周膜细胞中，PLAP-1是否参与调节胶原纤维的网络形成、牙周组织动态平衡等与牙周膜细胞密切相关的各种生理功能还有待进一步深入研究。