Formation of the periodontium begins following onset of tooth-root formation in a coordinated manner after birth. Dental follicle progenitor cells are thought to form the cementum, alveolar bone and Sharpey＇s fibers ...Formation of the periodontium begins following onset of tooth-root formation in a coordinated manner after birth. Dental follicle progenitor cells are thought to form the cementum, alveolar bone and Sharpey＇s fibers of the periodontal ligament （PDL）. However, little is known about the regulatory morphogens that control differentiation and function of these progenitor cells, as well as the progenitor cells involved in crown and root formation. We investigated the role of bone morphogenetic protein-2 （Bmp2） in these processes by the conditional removal of the Bmp2 gene using the Sp7-Cre-EGFP mouse model. Sp7-Cre-EGFP first becomes active at E18 in the first molar, with robust Cre activity at postnatal day 0 （PO）, followed by Cre activity in the second molar, which occurs after P0. There is robust Cre activity in the periodontium and third molars by 2 weeks of age. When the Bmp2gene is removed from Sp7＋ （Osterix＋） cells, major defects are noted in root, cellular cementum and periodontium formation. First, there are major cell autonomous defects in root-odontoblast terminal differentiation. Second, there are major alterations in formation of the PDLs and cellular cementum, correlated with decreased nuclear factor IC （Nfic）, periostin and α-SMA＋ cells. Third, there is a failure to produce vascular endothelial growth factor A （VEGF-A） in the periodontium and the pulp leading to decreased formation of the microvascular and associated candidate stem cells in the Bmp2-cKOsp7-cre＇EGFe. Fourth, ameloblast function and enamel formation are indirectly altered in the Bmp2-cKOsp7-cre＇EGFe. These data demonstrate that the Bmp2 gene has complex roles in postnatal tooth development and periodontium formation.展开更多
Objective:To observe the periodontal healing of autogenously transplanted teeth loaded orthodontically after autotransplantation in Beagle dogs.Methods:Forty-eight teeth were autogenously transplanted,24 of which were...Objective:To observe the periodontal healing of autogenously transplanted teeth loaded orthodontically after autotransplantation in Beagle dogs.Methods:Forty-eight teeth were autogenously transplanted,24 of which were loaded postoperatively with orthodontic force at different time points and for different durations.Periodontal healing was evaluated by probing pocket depth(PPD),the expression of relevant proteins,and histomorphometric analyses.Results:The dental pockets of loaded and non-loaded teeth were both much deeper after the first postoperative week than before transplantation(P<0.05).Later,the PPD,which was measured after postoperative weeks 1,3,5,9 and 13,gradually became shallow.The expressions of alkaline phosphatase(ALP)and basic fibroblast growth factor(bFGF)were higher in loaded teeth than in non-loaded teeth(P<0.05),and in groups subjected to two weeks duration of loading than in other groups at the same load time point(P<0.05).For the same load duration,the expressions of ALP and bFGF in teeth loaded after postoperative week 4 were higher than those of other treatments(P<0.05).According to histomorphometric analyses,an orthodontic force on transplanted teeth applied after postoperative weeks 4 or 8 for two weeks duration should be favorable for periodontal healing.Conclusions:It is advisable to apply an appropriate magnitude of force on autotransplanted teeth,such as orthodontic force,at appropriate time points and for a suitable duration,to achieve the optimal clinical prognosis following autogenous tooth transplantation.These results may serve as a basis for subsequent studies in humans so as to make clinical improvements.展开更多
目的:研究中重度慢性牙周炎患者采用激光辅助龈下刮治和根面平整术治疗对牙周组织和牙龈微循环的影响。方法:选取2018年12月-2020年12月常熟市第一人民医院收治的100例中重度慢性牙周炎患者,按随机数字表法分为两组。试验组(n=51)采取...目的:研究中重度慢性牙周炎患者采用激光辅助龈下刮治和根面平整术治疗对牙周组织和牙龈微循环的影响。方法:选取2018年12月-2020年12月常熟市第一人民医院收治的100例中重度慢性牙周炎患者,按随机数字表法分为两组。试验组(n=51)采取根面平整术(scaling and root planning,SRP)联合激光治疗,对照组(n=49)单纯应用SRP治疗。治疗前,治疗后2、4周患者均用激光多普勒血流仪(laser Doppler flowmetry,LDF)检查牙龈微循环,并对患者行牙周袋探诊深度(probing depth,PD)和探诊出血(bleeding on probing,BOP)检查。结果:治疗后2、4周,两组PD水平及BOP阳性率均较治疗前显著下降,且试验组均显著低于对照组(P<0.05);治疗后2、4周,与对照组比较,试验组织微血管血流灌注、活动血细胞浓度、微血管血流相对速度均显著升高,临床附着水平显著下降,差异均有统计学意义(P<0.05)。结论:Nd:YAG激光联合SRP治疗中重度慢性牙周炎的效果优于单独SRP治疗,其能更有效地清除菌斑及病变组织,改善患者牙周健康状况。展开更多
基金partly supported by research grant funding:NIH-NIAMS R01- AR054616 (SEH), NIH-NIDCR T32-DE14318 (Rakian) and F32-DE018865 (Yang)supported by UTHSCSA, NIH-NCI P30-CA54174 (CTRC at UTHSCSA) and NIH-NIA P01-AG19316supported by Open Fund of State Key Laboratory of Oral Diseases, Sichuan University
文摘Formation of the periodontium begins following onset of tooth-root formation in a coordinated manner after birth. Dental follicle progenitor cells are thought to form the cementum, alveolar bone and Sharpey＇s fibers of the periodontal ligament （PDL）. However, little is known about the regulatory morphogens that control differentiation and function of these progenitor cells, as well as the progenitor cells involved in crown and root formation. We investigated the role of bone morphogenetic protein-2 （Bmp2） in these processes by the conditional removal of the Bmp2 gene using the Sp7-Cre-EGFP mouse model. Sp7-Cre-EGFP first becomes active at E18 in the first molar, with robust Cre activity at postnatal day 0 （PO）, followed by Cre activity in the second molar, which occurs after P0. There is robust Cre activity in the periodontium and third molars by 2 weeks of age. When the Bmp2gene is removed from Sp7＋ （Osterix＋） cells, major defects are noted in root, cellular cementum and periodontium formation. First, there are major cell autonomous defects in root-odontoblast terminal differentiation. Second, there are major alterations in formation of the PDLs and cellular cementum, correlated with decreased nuclear factor IC （Nfic）, periostin and α-SMA＋ cells. Third, there is a failure to produce vascular endothelial growth factor A （VEGF-A） in the periodontium and the pulp leading to decreased formation of the microvascular and associated candidate stem cells in the Bmp2-cKOsp7-cre＇EGFe. Fourth, ameloblast function and enamel formation are indirectly altered in the Bmp2-cKOsp7-cre＇EGFe. These data demonstrate that the Bmp2 gene has complex roles in postnatal tooth development and periodontium formation.
基金Project supported by the Development of Science and Technology Project of Shandong Province(No.2008GG30002007)the International Technical Cooperation Plan of Shandong Province(No.2012GGE27143),China
文摘Objective:To observe the periodontal healing of autogenously transplanted teeth loaded orthodontically after autotransplantation in Beagle dogs.Methods:Forty-eight teeth were autogenously transplanted,24 of which were loaded postoperatively with orthodontic force at different time points and for different durations.Periodontal healing was evaluated by probing pocket depth(PPD),the expression of relevant proteins,and histomorphometric analyses.Results:The dental pockets of loaded and non-loaded teeth were both much deeper after the first postoperative week than before transplantation(P<0.05).Later,the PPD,which was measured after postoperative weeks 1,3,5,9 and 13,gradually became shallow.The expressions of alkaline phosphatase(ALP)and basic fibroblast growth factor(bFGF)were higher in loaded teeth than in non-loaded teeth(P<0.05),and in groups subjected to two weeks duration of loading than in other groups at the same load time point(P<0.05).For the same load duration,the expressions of ALP and bFGF in teeth loaded after postoperative week 4 were higher than those of other treatments(P<0.05).According to histomorphometric analyses,an orthodontic force on transplanted teeth applied after postoperative weeks 4 or 8 for two weeks duration should be favorable for periodontal healing.Conclusions:It is advisable to apply an appropriate magnitude of force on autotransplanted teeth,such as orthodontic force,at appropriate time points and for a suitable duration,to achieve the optimal clinical prognosis following autogenous tooth transplantation.These results may serve as a basis for subsequent studies in humans so as to make clinical improvements.
文摘目的:研究中重度慢性牙周炎患者采用激光辅助龈下刮治和根面平整术治疗对牙周组织和牙龈微循环的影响。方法:选取2018年12月-2020年12月常熟市第一人民医院收治的100例中重度慢性牙周炎患者,按随机数字表法分为两组。试验组(n=51)采取根面平整术(scaling and root planning,SRP)联合激光治疗,对照组(n=49)单纯应用SRP治疗。治疗前,治疗后2、4周患者均用激光多普勒血流仪(laser Doppler flowmetry,LDF)检查牙龈微循环,并对患者行牙周袋探诊深度(probing depth,PD)和探诊出血(bleeding on probing,BOP)检查。结果:治疗后2、4周,两组PD水平及BOP阳性率均较治疗前显著下降,且试验组均显著低于对照组(P<0.05);治疗后2、4周,与对照组比较,试验组织微血管血流灌注、活动血细胞浓度、微血管血流相对速度均显著升高,临床附着水平显著下降,差异均有统计学意义(P<0.05)。结论:Nd:YAG激光联合SRP治疗中重度慢性牙周炎的效果优于单独SRP治疗,其能更有效地清除菌斑及病变组织,改善患者牙周健康状况。
