INTRODUCTIONBifidobacteria are physiologically beneficial bacteria which are perdominant in human intestine ,and possess the most important functions .They play an important role in maintaining microbial balance of th...INTRODUCTIONBifidobacteria are physiologically beneficial bacteria which are perdominant in human intestine ,and possess the most important functions .They play an important role in maintaining microbial balance of the intestine .Furthermore , their presence is thought to be an important indication of health of the body [1-4].Whole peptidoglycan ( WPG) is the major component in the cell wall of bifidobacterium ,which is also a biological responsemodifier with nontoxic side dffcets.展开更多
AIM: To investigate the immunoregulatory effects of cinobufagin from the chloroform extract of Venenum Bufonis, a famous traditional Chinese medicine in vitro. METHODS: Proliferation of the splenic lymphocytes in Balb...AIM: To investigate the immunoregulatory effects of cinobufagin from the chloroform extract of Venenum Bufonis, a famous traditional Chinese medicine in vitro. METHODS: Proliferation of the splenic lymphocytes in Balb/c mice was measured by MTT assay. The metaboilic level and phagotrophic activity of PMΦ were also determined by MTT assay. The expressions of CD4 and CD8 and cell cycle of the splenic lymphocytes were detected by flow cytometry. RESULTS: Administration of cinobufagin or coadministration with Con A or LPS increased the cell proliferation and the percentage of S phase cells in the splenic lymphocytes at a dose-dependent manner(P<0.05 or P<0.01). Cinobufagin also enhanced the metabolic capability of MTT and the phagocytosis function of PMΦ(P<0.05 or P<0.01). In addition, it was found that the single administration of cinobufagin or coadministration with Con A or LPS also could increase expressions of CD4 and CD8 in T lymphocytes(P<0.05 or P<0.01). CONCLUSION: Single administration of cinobufagin or coadministration with Con A or LPS could enhance the reactive ability to the splenic lymphocytes and PMΦ in mice. The data indicate that the immunoregulatory effect of cinobufagin is related to the increased expression of CD4+ and CD8+ in DP (double positive) phenotype and S phase cells of T lymphocytes.展开更多
基金Supported by the Natural Science Foundation of Guangdong Province,No.994066
文摘INTRODUCTIONBifidobacteria are physiologically beneficial bacteria which are perdominant in human intestine ,and possess the most important functions .They play an important role in maintaining microbial balance of the intestine .Furthermore , their presence is thought to be an important indication of health of the body [1-4].Whole peptidoglycan ( WPG) is the major component in the cell wall of bifidobacterium ,which is also a biological responsemodifier with nontoxic side dffcets.
文摘AIM: To investigate the immunoregulatory effects of cinobufagin from the chloroform extract of Venenum Bufonis, a famous traditional Chinese medicine in vitro. METHODS: Proliferation of the splenic lymphocytes in Balb/c mice was measured by MTT assay. The metaboilic level and phagotrophic activity of PMΦ were also determined by MTT assay. The expressions of CD4 and CD8 and cell cycle of the splenic lymphocytes were detected by flow cytometry. RESULTS: Administration of cinobufagin or coadministration with Con A or LPS increased the cell proliferation and the percentage of S phase cells in the splenic lymphocytes at a dose-dependent manner(P<0.05 or P<0.01). Cinobufagin also enhanced the metabolic capability of MTT and the phagocytosis function of PMΦ(P<0.05 or P<0.01). In addition, it was found that the single administration of cinobufagin or coadministration with Con A or LPS also could increase expressions of CD4 and CD8 in T lymphocytes(P<0.05 or P<0.01). CONCLUSION: Single administration of cinobufagin or coadministration with Con A or LPS could enhance the reactive ability to the splenic lymphocytes and PMΦ in mice. The data indicate that the immunoregulatory effect of cinobufagin is related to the increased expression of CD4+ and CD8+ in DP (double positive) phenotype and S phase cells of T lymphocytes.
