Physiological Response and Resistance of Plants to Disease and Pest Stress

This paper outlines the physiological responses of plants to pathogenic microbial infection and pest feeding stress,as well as the resistance characteristics of plants to diseases and pests,and proposes new directions for future research on crop resistance to diseases and pests.The objective of this paper is to provide a reference framework for the breeding of crops with enhanced resistance to diseases and pests,the utilization of natural immunity in crops,and the efficient prevention and control of diseases and pests.This framework is intended to facilitate the healthy and sustainable development of the agricultural industry.

High Temperature Effects on Yeast-like Endosymbiotes and Pesticide Resistance of the Small Brown Planthopper, Laodelphax striatellus

The newly-hatched nymphs of the small brown planthopper (SBPH), Laodelphax striatellus, including field and sensitive populations, were subjected to the high-temperature (35°C) treatment. The number of yeast-like endosymbiotes in SBPH reduced by 23.47%–34.23%, 57.86%–61.51% and 88.96%–90.71% after the high-temperature treatment for 1 d, 2 d, and 3 d, respectively. However, the size of yeast-like endosymbiotes was not obviously affected. Resistance of SBPH to three insecticides (imidacloprid, chlorpyrifos and fipronil) decreased with the increase of treatment time.

Genetic Inheritance and Molecular Marker of Clubroot Resistance Genes in Brassica campestris ssp. chinensis

Objective] This study was conducted to investigate the genetic inheritance of clubroot resistance in Chinese non-heading cabbage （Brassica campestris ssp. chinensis）. [Method] The clubroot resistance gene was introduced from a Brassica campestris ssp. pekinensis cultivar to non-heading Chinese cabbage, and the inheri-tance and molecular markers of clubroot resistance gene in parental lines, F1, F2 and BC1 of non-heading Chinese cabbage were studied through pathogen inoculation at seedling stage and ISSR-PCR. [Result] Clubroot resistance in non-heading Chi-nese cabbage was control ed by a single dominant gene. ISSR molecular markers with Bulk segregant analysis （BSA） found that primer-873 was linked to resistance gene, named CR-873, and the genetic distance between the marker and the resis-tance gene was 9.72 cM. [Conclusion] The results provide references for the molecular marker assisted breeding of non-heading Chinese cabbage.

Development of Marker-Free Transgenic Cry1Ab Rice with Lepidopteran Pest Resistance by Agrobacterium Mixture-Mediated Co-transformation

CrylAb gene was transformed into four rice varieties, Zhejing 22, Zhejing 27, Jiahua 1 and Xiushui 63 mediated by Agrobacterium-mixture co-transformation. Rice genotype had an important effect on callus induction and transformation efficiency. Different mixtures of Agrobacterium strains （EHA105 and EHA101） contained Hpt and CrylAb genes resulted in different frequencies of resistant calli. There was no correlation between the frequency of transformants with the ratio of the Agrobacterium strain mixture contained Hpt and CrylAb genes. A total of 509 transgenic plants were obtained from the four rice varieties, and 272 T2 progenies were analyzed for CrylAb and Hpt genes. PCR analysis revealed that 412 regenerated plants were Hpt positive （80.94%）, 62 plants were also CrylAb co-transformants （15.05% in total frequency）, and 42 plants among the 272 T2 progenies were CrylAb positive but Hpt negative. This suggests that marker-free transgenic plants could be produced by co-transformation mediated by mixed Agrobacterium strains with the selectable marker gene and target gene Southern blot analysis of five independent marker-free T2 transgenic lines co-transformed from Zhejing 22 showed that CrylAb gene had been inserted into rice genome with a single copy. The transgenic plants showed significantly stronger resistance to lepidopteron than the non-transgenic plants under no application of insecticides against lepidopteron.

Plant Resistance to Pest and Research Progress of Its Genetic Improvement

Pest damage is a significant factor that cuts agricultural production, and cultivation of pest-resistant varieties is the most economic and effective approach of controlling pest damage. Plant pest resistance includes antixenosis, antibiosis, and tolerance. Current popular methods for the genetic improvement of crop pest resistance are traditional breeding method, transgenic breeding, and molecular mark-assisted breeding. This paper introduced major mechanisms and genetic bases of plant pest resistance, ＂and reviewed research progress of domestic and international genetic improvement of plant pest resistance, analyzed the problems and its de- velopment prospects.

Genetically Engineered Corn Rootworm Resistance: Potential for Reduction of Human Health Effects From Pesticides

Objective and Methods Insecticide use, grower preferences regarding genetically engineered (GE) corn resistant to com rootworm (CRW), and the health effects of using various CRW insecticides (organophosphates, pyrethroids, fipronil and carbamates) are reviewed for current and future farm practices. Results Pest damage to corn has been reduced only one-third by insecticide applications. Health costs from insecticide use appear significant, but costs attributable to CRW control are not quantifiable from available data. Methods reducing health-related costs of insecticide-based CRW control should be evaluated. As a first step, organophosphate insecticide use has been reduced as they have high acute toxicity and risk of long-term neurological consequences. A second step is to use agents which more specifically target the CRW. Conclusion Whereas current insecticides may be poisonous to many species of insects, birds, mammals and humans, a protein derived from Bacillus thurigiensis and produced in plants via genetic modification can target the specific insect of CRW (Coleoptra), sparing other insect and non-insect species from injury.

Resistance Monitoring of Field Populations of Panonychus citri to Three Pesticides

[Objectives]The paper was to investigate the development of Panonychus citri resistance to commonly used pesticides in major citrus producing areas in China,and to screen out new green and efficient agents for its control and prevention.[Methods]The resistance changes of field populations of P.citri to abamectin,pyridaben,and bifenazate in 12 locations across five provinces in China were compared using the leaf disc impregnation method.[Results]P.citri in the tested areas exhibited the most severe resistance to abamectin,with approximately 91.7%of the field populations showing high levels of resistance to abamectin(112.1-560.5 times);50%of P.citri populations exhibited high levels of resistance to pyridaben(123.0-202.7 times),while the remaining populations showed intermediate levels of resistance(25.6-80.3 times);except for the Zhejiang Xiangshan 2019 and Jiangxi Yudu 2019 populations,which exhibited a medium level of resistance to bifenazate,the remaining 10 monitored populations demonstrated a high level of resistance to bifenazate(140.4-686.1 times).[Conclusions]It is advisable to discontinue the use of abamectin and bifenazate due to significant resistance observed in populations of P.citri monitored in 12 locations across 5 provinces.It is recommended to reduce the frequency of pyridaben use and alternate with other acaricides that have different mechanisms of action due to the varying degrees of resistance developed.

A Review of Agricultural Pesticides Use and the Selection for Resistance to Insecticides in Malaria Vectors

Most national malaria control programmes rely extensively on pyrethroid insecticides to control mosquito vectors of this disease. Unfortunately, the intensive use of this class of insecticides both in public health and agriculture has led to its reduced efficacy. The objective of this review was to assess the role of agricultural pesticides use on the development of resistance to insecticides in malaria vectors and the potential impact of this resistance on control activities. We searched library catalogues and public databases for studies that included data on resistance to the major classes of insecticides: organochlorines, carbamates, organophosphates and pyrethroids, in the malaria vectors of Anopheles genera. There is a strong geographical bias in published studies many originating from West African countries. Several studies demonstrate that resistance to pyrethroids is widespread in the major malaria vectors of the Anopheles gambiae and Anopheles funestus complexes. Assessing the impact of insecticide resistance on vector control is complicated owing to the lack of studies into the epidemiological consequences of resistance on the control of malaria and other vector borne diseases.

Comparison of Positions of QTLs Conferring Resistance to <i>Xanthomonas campestris</i>pv. <i>campestris</i>in <i>Brassica oleracea</i>

Black rot, caused by Xanthomonas campestris pv. campestris (Xcc) is possibly the most important disease of Brassica worldwide. To compare chromosomal positions of Xcc resistance loci in Brassica oleracea between the present and published studies and to develop marker assisted selection (MAS) to resistance against Xcc race 1, we constructed a B. oleracea map, including pW, pX and BoCL markers that were closely linked to previously reported Xcc resistance QTLs. We also analyzed Xcc resistance QTLs by improving our previously reported map derived from the cross of a susceptible double-haploid line (GC P09) with a resistant double-haploid line (Reiho P01). In the nine linkage groups obtained (C1-C9), the major QTL, XccBo(Reiho)2, was derived from Reiho with a maximum LOD score (7.7) in C8. The QTL (LOD 4.4) located in C9, XccBo(GC)1 was derived from the susceptible GC. The other QTL (LOD 4.4), XccBo(Reiho)1, was found in C5. Based on common markers, it was possible to compare our finding Xcc resistance QTLs with the B. oleraceaXcc loci reported by previous authors;XccBo(Reiho)1 and XccBo(GC)1 may be identical to the Xcc resistance QTLs reported previously or a different member contained in the same resistance gene cluster. Our map includes public SSR markers linked to Xcc resistance genes that will promote pyramiding Xcc resistance genes in B. oleracea. The present study will also contribute to a better understanding of genetic control of Xcc resistance.

Evaluation of cotton germplasm for morphological and biochemical host plant UPdates resistance traits against sucking insect pests complex

Background:Sucking insect pests cause severe damage to cotton crop production.The development of insect resistant cotton cultivars is one of the most effective measures in curtailing the yield losses.Considering the role of morphological and biochemical host plant resista nee(HPR)traits in plant defense,12 cotton genotypes/varieties were evaluated for leaf area,leaf glanding,total soluble sugars,total soluble proteins,total phenolics,tannin and total flavonoids against fluctuating populations of whitefly,thrips and jassid under field conditions.Results:The population of these insects fluctuated during the growing seas on and remained above threshold level(whitefly>5,thrips>(8-10)f or jassid>1 per leaf)during late June and early July.Strong and negative association of whitefly(r=-0.825)and jassid(r=-0.929)with seed cotton yield was observed.Mean population of insects were the highest in Glandless-1 followed by NIA-82 and NIA-M30.NIAB-Kiran followed by NI AB-878 and Sadori were the most resistant,with the mean population of 1.41,1.60,1.66(whitefly);2.24,232,2.53(thrips)and 037,0.31,036(jassid),respectively.The resistant variety NIAB-Kiran showed less soluble sugars(8.54 mg.g^(-1)),soluble proteins(27.11 mg.g^(-1))and more phenolic(36.56 mg.g^(-1))and flavonoids(13.10mg.g^(-1))as compared with the susceptible check Glandless-1.Moreover,all insect populations were positively correlated with total soluble sugars and proteins.Whitefly populations exhibited negative response to leaf gossypol glands,total phenolics,tannins and flavonoids.The thrips and jassid populations had a significant and negative correlation with these four biochemical HPR traits.Conclusion:The ide ntified resistant resources and HPR traits can be deployed against sucking in sect pests'complex in future breeding programs of developing insect resistant cotton varieties.

Advance of Studies on Pesticides Resistance of Parasitoids

抗性寄生蜂的应用是IPM中生防和化防相结合的重要措施之一。本文综述了寄生蜂与杀虫剂的关系、寄生蜂抗药性与其性别的关系、寄生蜂抗药性的稳定性及其抗性机理。

Evaluation and screening for resistance of some varieties(lines)to major diseases and insect pests of rice

During 1984-1988,2,231 varieties(lines)from International Rice Testing Program(IRTP)were evaluated and screened for resistance to riceblast(Bl),bacterial blight(BB),sheath blight

Occurrence of Major Diseases and Insect Pests in Medium-term Growth Period of Sugarcane and The Diseases Resistance Evaluation in Guangxi

To figure out major diseases and insect pests of sugarcane and sugarcane resistance to diseases,the occurrence of major diseases and insect pests on 11 main sugarcane varieties in eight sugarcane producing areas of Guangxi was investigated during the medium-term growth period of sugarcane in 2015,and field resistance to smut and mosaic diseases was evaluated. The results showed that the dead heart rate（ DHR） ranged from 0. 00% to 38. 55%; new-planting sugarcane of Guiliu 2 and ratoon sugarcane of Liucheng 05-136 suffered the most severe damage in Beihai city,and the DHR were 38. 55% and 37. 66%,respectively. The incidence rate of smut disease ranged from 0. 00% to 24. 89%. Ratoon sugarcane was more susceptible to smut than new-planting sugarcane among all investigated varieties. Variety ROC22 was most susceptible to smut,and performed moderate susceptibility. Guiliu 2 was the second susceptible and performed resistance,with the incidence rate of 7. 45%. The rest nine varieties had high resistance. Sugarcane mosaic disease occurred mildly in Guangxi,and the incidence rate ranged from 0. 39% to 7. 73%. ROC22,Guitang 42 and Guiliu 2 expressed moderate resistance and the rests exhibited resistance. Sugarcane in Nanning and Beihai suffered more severe damage than those in other producing areas. The field evaluation system of sugarcane resistance to borers was expected to be constructed. ROC22 was most susceptible to smut,compared with other main varieties. Mosaic diseases occurred lightly in Guangxi. Multi-location and multi-year observation are necessary for accurate evaluation of sugarcane resistance to smut and mosaic diseases.

基于SWOT和PEST分析的模块化麻醉学技能培训教学模型应用

目的探讨基于SWOT和PEST分析的模块化配置麻醉学临床技能培训教学模型在研究生培训过程中的应用。方法选取2022年12月至2023年8月在郑州大学第一附属医院麻醉临床轮转的80名研究生,随机分为观察组(n=40)和对照组(n=40)。对照组采用传统教学方法,观察组采取基于SWOT和PEST分析的模块化配置麻醉学临床技能培训教学模型教学,比较两组研究生的技能考试成绩、案例分析成绩、基础理论成绩,以及对教学满意度评分。结果观察组研究生培训后的技能考试、案例分析、基础理论成绩均高于对照组,差异均有统计学意义(t分别=7.18、8.44、8.69,P均<0.05),观察组带教满意度评分高于对照组,差异有统计学意义(t=18.91,P<0.05)。结论基于SWOT和PEST分析的模块化配置麻醉学临床技能培训教学模型在研究生技能培训中的应用可有效提高临床技能水平和综合应用能力,有助于实践能力和临床思维的培养。

DRG/DIP模式下日间手术运营管理创新PEST分析

目的运用PEST分析的创新方法,对DRG/DIP模式下日间手术运营管理进行探索,以期为管理者提供新的思路。方法根据PEST分析框架,提取关键指标进行分析,根据上述数据和文献分析,提出创新的日间手术运营管理策略。结果需要推动DRG/DIP政策的修订和完善,同时加强手术管理规范,以促进日间手术管理创新;合理分配医疗资源,降低成本,提高综合效益;深入了解患者需求,提高服务质量;加强日间手术管理信息平台建设和全程管理与监测,积极开展新技术,满足患者高品质医疗服务需求。结论推动日间手术持续高质量发展,必须完善相关规范和制度,加强全流程管理。

Building a cluster of NLR genes conferring resistance to pests and pathogens: the story of the Vat gene cluster in cucurbits

Most molecularly characterized plant resistance genes(R genes)belong to the nucleotide-binding-site-leucine-rich-repeat(NLR)receptor family and are prone to duplication and transposition with high sequence diversity.In this family,the Vat gene in melon is one of the few R genes known for conferring resistance to insect,i.e.,Aphis gossypii,but it has been misassembled and/or mispredicted in the whole genomes of Cucurbits.We examined 14 genomic regions(about 400 kb)derived from long-read assemblies spanning Vat-related genes in Cucumis melo,Cucumis sativus,Citrullus lanatus,Benincasa hispida,Cucurbita argyrosperma,and Momordica charantia.We built the phylogeny of those genes.Investigating the paleohistory of the Vat gene cluster,we revealed a step by step process beginning from a common ancestry in cucurbits older than 50 my.We highlighted Vat exclusively in the Cucumis genera,which diverged about 20 my ago.We then focused on melon,evaluating a minimum duplication rate of Vat in 80 wild and cultivated melon lines using generalist primers;our results suggested that duplication started before melon domestication.The phylogeny of 44 Vat-CDS obtained from 21 melon lines revealed gain and loss of leucine-rich-repeat domains along diversification.Altogether,we revealed the high putative recognition scale offered in melon based on a combination of SNPs,number of leucine-rich-repeat domains within each homolog and number of homologs within each cluster that might jointly confer resistance to a large pest and pathogen spectrum.Based on our findings,we propose possible avenues for breeding programs.

基于PEST模型的主要发达国家农业气象服务发展及其启示

近年来气候变化带来的负面影响对农业生产产生了切实的影响,各国采取了系统性的措施来更好地应对气候变化的挑战,确保国家粮食安全和农业可持续发展。文中采用PEST模型系统分析了美国、欧洲和以色列等发达国家/地区及农业特色国家在农业气象服务发展上的最新进展和成功经验,结论:1) 在政治方面(Political),这些国家通过完善法律法规体系和优化政策,提供了强大的政策支持和法律保障;2) 在经济方面(Economic),通过农业气象资金支持和农业气象保险大力推动农业气象服务的发展,并为农业从业者应对气象灾害提供了充足的资金保障;3) 在社会方面(Social),通过打造免费共享的高质量农业气象信息服务系列产品和高度重视农业教育,扩大了农业气象服务的影响力并提升了农业从业人员的素质;4) 在技术方面(Technological),通过建立完善的农业气象观测网络,研发改进作物生长模拟模型和推广气候智能型农业,有效地保障了农业气象服务的效率和效益,并推动了农业的绿色可持续发展。这些经验对于促进我国农业气象服务发展具有重要的借鉴意义,能够为推动我国农业气象服务高质量发展提供一定的参考。

Nature’s Pharmacy under Siege: Investigating Antibiotic Resistance Pattern in Endophytic Bacteria of Medicinal Plants

Antibiotic resistance poses a significant global health threat, necessitating a thorough understanding of its prevalence in various ecological contexts. Medicinal plants, renowned for their therapeutic properties, host endophytic bacteria that produce bioactive compounds. Understanding antibiotic resistance dynamics in these bacteria is vital for human health and antibiotic efficacy preservation. In this study, we investigated antibiotic resistance profiles in endophytic bacteria from five medicinal plants: Thankuni, Neem, Aparajita, Joba, and Snake plant. We isolated and characterized 113 endophytic bacteria, with varying resistance patterns observed against multiple antibiotics. Notably, 53 strains were multidrug-resistant (MDR), with 14 exhibiting extensive drug resistance (XDR). Thankuni-associated bacteria displayed 44% MDR and 11% XDR, while Neem-associated bacteria showed higher resistance (60% MDR, 13% XDR). Aparajita-associated bacteria had lower resistance (22% MDR, 6% XDR), whereas Joba-associated bacteria exhibited substantial resistance (54% MDR, 14% XDR). Snake plant-associated bacteria showed 7% MDR and 4% XDR. Genus-specific distribution revealed Bacillus (47%), Staphylococcus (21%), and Klebsiella (11%) as major contributors to MDR. Our findings highlight diverse drug resistance patterns among plant-associated bacteria and underscore the complexity of antibiotic resistance dynamics in diverse plant environments. Identification of XDR strains emphasizes the severity of the antibiotic resistance problem, warranting further investigation into contributing factors.

Five-year analysis of isolated pathogens and antibiotic resistance of ocular infections from two large tertiary comprehensive hospitals in east China

AIM:To analyze the spectrum of isolated pathogens and antibiotic resistance for ocular infections within 5y at two tertiary hospitals in east China.METHODS:Ocular specimen data were collected from January 2019 to October 2023.The pathogen spectrum and positive culture rate for different infection location,such as keratitis,endophthalmitis,and periocular infections,along with antibiotic resistance were analyzed.RESULTS:We included 2727 specimens,including 827(30.33%)positive cultures.A total of 871 strains were isolated,530(60.85%)bacterial and 341(39.15%)fungal strains were isolated.Gram-positive cocci(GPC)were the most common ocular pathogens.The most common bacterial isolates were Staphylococcus epidermidis(25.03%),Staphylococcus aureus(7.46%),Streptococcus pneumoniae(4.59%),Corynebacterium macginleyi(3.44%),and Pseudomonas aeruginosa(3.33%).The most common fungal genera were Fusarium spp.(12.74%),Aspergillus spp.(6.54%),and Scedosporium spp.(5.74%).Staphylococcus epidermidis strains showed more than 50%resistance to fluoroquinolones.Streptococcus pneumoniae and Corynebacterium macginleyi showed more than 90%resistance to erythromycin.The percentage of bacteria showing multidrug resistance(MDR)significantly decreased(χ^(2)=17.44,P=0.002).CONCLUSION:GPC are the most common ocular pathogens.Corynebacterium macginleyi,as the fourth common bacterium,may currently be the local microbiological feature of east China.Fusarium spp.is the most common fungus.More than 50%of the GPC are resistant to fluoroquinolones,penicillins,and macrolides.However,the proportion of MDR strains has been reduced over time.

Enhanced wear resistance,antibacterial performance,and biocompatibility using nanotubes containing nano-Ag and bioceramics in vitro

A good Ti-based joint implant should prevent stress shielding and achieve good bioactivity and anti-infection performance.To meet these requirements,the low-elastic-modulus alloy—Ti–35Nb–2Ta–3Zr—was used as the substrate,and functional coatings that contained bioceramics and Ag ions were prepared for coating on TiO_(2)nanotubes(diameter:(80±20)nm and(150±40)nm)using anodization,deposition,and spin-coating methods.The effects of the bioceramics(nano-β-tricalcium phosphate,microhydroxyapatite(micro-HA),and meso-CaSiO_(3))and Ag nanoparticles(size:(50±20)nm)on the antibacterial activity and the tribocorrosion,corrosion,and early in vitro osteogenic behaviors of the nanotubes were investigated.The tribocorrosion and corrosion results showed that the wear rate and corrosive rate were highly dependent on the features of the nanotube surface.Micro-HA showed great wear resistance with a wear rate of(1.26±0.06)×10^(−3)mm^(3)/(N·m)due to adhesive and abrasivewear.Meso-CaSiO_(3)showed enhanced cell adhesion,proliferation,and alkaline phosphatase activity.The coatings that contained nano-Ag exhibited good antibacterial activity with an antibacterial rate of≥89.5%against Escherichia coli.These findings indicate that hybrid coatings may have the potential to accelerate osteogenesis.