In this paper, the existence of strictly positive solutions for N-species nonau- tonom ous Kolm ogorov com petition system s is studied. By applying the Schauder's fixed point theorem som e new sufficient condit...In this paper, the existence of strictly positive solutions for N-species nonau- tonom ous Kolm ogorov com petition system s is studied. By applying the Schauder's fixed point theorem som e new sufficient conditions are established. In particular, for the alm ost periodic system , the existence of strictly positive alm ostperiodic solutions is obtained. Som e previous results are im proved and generalized.展开更多
TWENTY one-year-old Fan Chunmei,a singer from an artensemble of the Chinese People‘s Liberation Army,won firstprize in the fourth Sound of Asia International Popular SongCompetition,held in Kazakhstan on July 19.Ai J...TWENTY one-year-old Fan Chunmei,a singer from an artensemble of the Chinese People‘s Liberation Army,won firstprize in the fourth Sound of Asia International Popular SongCompetition,held in Kazakhstan on July 19.Ai Jing,from LiaoningProvince,won the Alma-Ata Mayor’s Prize.She sang“My 1997.”Yuan Chenyie of the China Opera Theater took second prize inthe Classical division of the Tokyo International Vocal Music Com-petition,held in Tokyo in June this year.展开更多
[Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PP...[Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PPR.[Methods]Soluble N protein and NH fusion protein were successfully obtained in an Escherichia coli expression system by optimizing E.coli codon and expression conditions.Furthermore,based on purified soluble N protein and NH fusion protein,a double-antigen sandwich time-resolved fluorescence immunoassay method for detection of peste des petits ruminants virus(PPRV)was established.[Results]The method has high sensitivity and specificity and can specifically detect the antibody against PPRV in sheep serum,and it has no cross reaction with other related diseases.The method was used to detect 292 clinical samples,and compared with French IDVET competition ELISA kit.The coincidence rates of positive samples and negative samples from the two kinds of test kits were 92.47%and 97.26%,respectively,and the overall coincidence rate was 94.86%.The intra-group and inter-group coefficients of variation in the repeatability test were less than 10%.[Conclusions]Compared with the traditional ELISA method,the double-antigen sandwich time-resolved fluorescence immunoassay for detection of PPRV has equivalent sensitivity and specificity,and simple and rapid operation,and thus high application and popularization value.展开更多
[Objectives]This study was conducted to establish a rapid quantitative method for detecting antibody against Peste des Petits Ruminants Virus(PPR V)in sheep serum.[Methods]Soluble N protein and NH fusion protein were ...[Objectives]This study was conducted to establish a rapid quantitative method for detecting antibody against Peste des Petits Ruminants Virus(PPR V)in sheep serum.[Methods]Soluble N protein and NH fusion protein were obtained in Escherichia coli prokaryotic expression system by optimizing codons and expression conditions of E.coli.Furthermore,based on the purified soluble N protein and NH fusion protein,a high-sensitivity fluorescence immunoassay kit for detecting the antibody against PPR V was established.[Results]The method could quickly and quantitatively detect PPR V antibody in sheep serum,with high sensitivity and specificity,without any cross reaction to other related sheep pathogens.The intra-batch and inter-batch coefficients of variation were less than 10%and 15%,respectively,and the method had good repeatability.Through detection on 292 clinical serum samples,it was compared with the French IDVET competitive ELISA kit,and the coincidence rate of the two methods reached 93.84%.Compared with the serum neutralization test,the detected titer value of the high-sensitivity rapid fluorescence quantitative detection method was basically consistent with the tilter value obtained by the neutralization test on the standard positive serum(provided by the WOAH Brucellosis Reference Laboratory of France).[Conclusions]This method can realize rapid quantitative detection of PPR V antibody on site,and has high practical value and popularization value.展开更多
近日,2016年度睡莲新品种比赛（N ew W aterlily C om petition）获奖名单出炉：‘舞会皇后’及‘少女’获耐寒睡莲类冠、亚军;‘维吉尼亚·麦克莱恩’及‘星系’获热带睡莲类冠、亚军;‘侦探艾丽卡’及‘玛蒂妮娜’获亚属间杂交种...近日,2016年度睡莲新品种比赛（N ew W aterlily C om petition）获奖名单出炉：‘舞会皇后’及‘少女’获耐寒睡莲类冠、亚军;‘维吉尼亚·麦克莱恩’及‘星系’获热带睡莲类冠、亚军;‘侦探艾丽卡’及‘玛蒂妮娜’获亚属间杂交种睡莲类冠、亚军,另外,‘侦探艾丽卡’及‘维吉尼亚·麦克莱恩’获年度最佳新品种睡莲冠、亚军（图1）。笔者育成的睡莲新品种‘侦探艾丽卡’在此比赛中成为年度双料冠军,并在国际睡莲与水景园协会获得了国际权威登录。展开更多
In response to the UnitedNations’call for carrying out theInternational Space Year(ISY)ac-tivities,China ISY PreparatoryCommittee.(CPC)has been estab-lished with state councillor
Recently,cotton textile enterprises find their cotton shortage problem is getting worse and worse.After 34 textile enterprises in Henan Province drew up a petition to relevant state departments,about 150 textile enter...Recently,cotton textile enterprises find their cotton shortage problem is getting worse and worse.After 34 textile enterprises in Henan Province drew up a petition to relevant state departments,about 150 textile enterprises in Hubei,Hunan,Shandong,Hebei,Zhejiang,Fujian,Jiangsu,Guangdong and other places recently reported the problems of cotton reserve by telephone or in form of petition to China Cotton Textile Association,hoping to advise this issue展开更多
文摘In this paper, the existence of strictly positive solutions for N-species nonau- tonom ous Kolm ogorov com petition system s is studied. By applying the Schauder's fixed point theorem som e new sufficient conditions are established. In particular, for the alm ost periodic system , the existence of strictly positive alm ostperiodic solutions is obtained. Som e previous results are im proved and generalized.
文摘TWENTY one-year-old Fan Chunmei,a singer from an artensemble of the Chinese People‘s Liberation Army,won firstprize in the fourth Sound of Asia International Popular SongCompetition,held in Kazakhstan on July 19.Ai Jing,from LiaoningProvince,won the Alma-Ata Mayor’s Prize.She sang“My 1997.”Yuan Chenyie of the China Opera Theater took second prize inthe Classical division of the Tokyo International Vocal Music Com-petition,held in Tokyo in June this year.
基金Supported by National Key R&D Program for the Prevention and Control of Major Exotic Animal Diseases(2022YFD1800500)National Mutton Sheep Industrial Technology System(CARS39)+2 种基金Key Research and Development Program of Shandong Province(Major Science and Technology Innovation Project)(2021CXGC011306)Scientific Research Project of General Administration of Customs(2024HK033)Scientific Research Project of Jinan Customs(2023JK005).
文摘[Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PPR.[Methods]Soluble N protein and NH fusion protein were successfully obtained in an Escherichia coli expression system by optimizing E.coli codon and expression conditions.Furthermore,based on purified soluble N protein and NH fusion protein,a double-antigen sandwich time-resolved fluorescence immunoassay method for detection of peste des petits ruminants virus(PPRV)was established.[Results]The method has high sensitivity and specificity and can specifically detect the antibody against PPRV in sheep serum,and it has no cross reaction with other related diseases.The method was used to detect 292 clinical samples,and compared with French IDVET competition ELISA kit.The coincidence rates of positive samples and negative samples from the two kinds of test kits were 92.47%and 97.26%,respectively,and the overall coincidence rate was 94.86%.The intra-group and inter-group coefficients of variation in the repeatability test were less than 10%.[Conclusions]Compared with the traditional ELISA method,the double-antigen sandwich time-resolved fluorescence immunoassay for detection of PPRV has equivalent sensitivity and specificity,and simple and rapid operation,and thus high application and popularization value.
基金Supported by The National Project for the Prevention and Control of Major Exotic Animal Diseases(2022YFD1800500)National Mutton Sheep Industrial Technology System(CARS39).
文摘[Objectives]This study was conducted to establish a rapid quantitative method for detecting antibody against Peste des Petits Ruminants Virus(PPR V)in sheep serum.[Methods]Soluble N protein and NH fusion protein were obtained in Escherichia coli prokaryotic expression system by optimizing codons and expression conditions of E.coli.Furthermore,based on the purified soluble N protein and NH fusion protein,a high-sensitivity fluorescence immunoassay kit for detecting the antibody against PPR V was established.[Results]The method could quickly and quantitatively detect PPR V antibody in sheep serum,with high sensitivity and specificity,without any cross reaction to other related sheep pathogens.The intra-batch and inter-batch coefficients of variation were less than 10%and 15%,respectively,and the method had good repeatability.Through detection on 292 clinical serum samples,it was compared with the French IDVET competitive ELISA kit,and the coincidence rate of the two methods reached 93.84%.Compared with the serum neutralization test,the detected titer value of the high-sensitivity rapid fluorescence quantitative detection method was basically consistent with the tilter value obtained by the neutralization test on the standard positive serum(provided by the WOAH Brucellosis Reference Laboratory of France).[Conclusions]This method can realize rapid quantitative detection of PPR V antibody on site,and has high practical value and popularization value.
文摘近日,2016年度睡莲新品种比赛（N ew W aterlily C om petition）获奖名单出炉：‘舞会皇后’及‘少女’获耐寒睡莲类冠、亚军;‘维吉尼亚·麦克莱恩’及‘星系’获热带睡莲类冠、亚军;‘侦探艾丽卡’及‘玛蒂妮娜’获亚属间杂交种睡莲类冠、亚军,另外,‘侦探艾丽卡’及‘维吉尼亚·麦克莱恩’获年度最佳新品种睡莲冠、亚军（图1）。笔者育成的睡莲新品种‘侦探艾丽卡’在此比赛中成为年度双料冠军,并在国际睡莲与水景园协会获得了国际权威登录。
文摘In response to the UnitedNations’call for carrying out theInternational Space Year(ISY)ac-tivities,China ISY PreparatoryCommittee.(CPC)has been estab-lished with state councillor
文摘Recently,cotton textile enterprises find their cotton shortage problem is getting worse and worse.After 34 textile enterprises in Henan Province drew up a petition to relevant state departments,about 150 textile enterprises in Hubei,Hunan,Shandong,Hebei,Zhejiang,Fujian,Jiangsu,Guangdong and other places recently reported the problems of cotton reserve by telephone or in form of petition to China Cotton Textile Association,hoping to advise this issue
