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Fabrication and integration of photonic devices for phase-change memory and neuromorphic computing 被引量:1
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作者 Wen Zhou Xueyang Shen +2 位作者 Xiaolong Yang Jiangjing Wang Wei Zhang 《International Journal of Extreme Manufacturing》 SCIE EI CAS CSCD 2024年第2期2-27,共26页
In the past decade,there has been tremendous progress in integrating chalcogenide phase-change materials(PCMs)on the silicon photonic platform for non-volatile memory to neuromorphic in-memory computing applications.I... In the past decade,there has been tremendous progress in integrating chalcogenide phase-change materials(PCMs)on the silicon photonic platform for non-volatile memory to neuromorphic in-memory computing applications.In particular,these non von Neumann computational elements and systems benefit from mass manufacturing of silicon photonic integrated circuits(PICs)on 8-inch wafers using a 130 nm complementary metal-oxide semiconductor line.Chip manufacturing based on deep-ultraviolet lithography and electron-beam lithography enables rapid prototyping of PICs,which can be integrated with high-quality PCMs based on the wafer-scale sputtering technique as a back-end-of-line process.In this article,we present an overview of recent advances in waveguide integrated PCM memory cells,functional devices,and neuromorphic systems,with an emphasis on fabrication and integration processes to attain state-of-the-art device performance.After a short overview of PCM based photonic devices,we discuss the materials properties of the functional layer as well as the progress on the light guiding layer,namely,the silicon and germanium waveguide platforms.Next,we discuss the cleanroom fabrication flow of waveguide devices integrated with thin films and nanowires,silicon waveguides and plasmonic microheaters for the electrothermal switching of PCMs and mixed-mode operation.Finally,the fabrication of photonic and photonic–electronic neuromorphic computing systems is reviewed.These systems consist of arrays of PCM memory elements for associative learning,matrix-vector multiplication,and pattern recognition.With large-scale integration,the neuromorphic photonic computing paradigm holds the promise to outperform digital electronic accelerators by taking the advantages of ultra-high bandwidth,high speed,and energy-efficient operation in running machine learning algorithms. 展开更多
关键词 nanofabrication silicon photonics phase-change materials non-volatile photonic memory neuromorphic photonic computing
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Theoretical analysis of nanosecond crystallization kinetics of phase-change optical recording films
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作者 张学如 杨昆 宋瑛林 《Chinese Physics B》 SCIE EI CAS CSCD 2005年第9期1900-1903,共4页
A theoretical investigation of nanosecond crystallization kinetics of the phase-change optical recording films is presented. An extended Kissinger equation for the square-root heating is derived, which properly descri... A theoretical investigation of nanosecond crystallization kinetics of the phase-change optical recording films is presented. An extended Kissinger equation for the square-root heating is derived, which properly describes the temperature evolution of the films by nanosecond laser heating. The extended Kissinger equation was used to explain our previous experimental results. 展开更多
关键词 phase-change crystallization kinetics activation energy
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Detection of Turbulence Anomalies Using a Symbolic Classifier Algorithm in Airborne Quick Access Record(QAR)Data Analysis 被引量:1
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作者 Zibo ZHUANG Kunyun LIN +1 位作者 Hongying ZHANG Pak-Wai CHAN 《Advances in Atmospheric Sciences》 SCIE CAS CSCD 2024年第7期1438-1449,共12页
As the risks associated with air turbulence are intensified by climate change and the growth of the aviation industry,it has become imperative to monitor and mitigate these threats to ensure civil aviation safety.The ... As the risks associated with air turbulence are intensified by climate change and the growth of the aviation industry,it has become imperative to monitor and mitigate these threats to ensure civil aviation safety.The eddy dissipation rate(EDR)has been established as the standard metric for quantifying turbulence in civil aviation.This study aims to explore a universally applicable symbolic classification approach based on genetic programming to detect turbulence anomalies using quick access recorder(QAR)data.The detection of atmospheric turbulence is approached as an anomaly detection problem.Comparative evaluations demonstrate that this approach performs on par with direct EDR calculation methods in identifying turbulence events.Moreover,comparisons with alternative machine learning techniques indicate that the proposed technique is the optimal methodology currently available.In summary,the use of symbolic classification via genetic programming enables accurate turbulence detection from QAR data,comparable to that with established EDR approaches and surpassing that achieved with machine learning algorithms.This finding highlights the potential of integrating symbolic classifiers into turbulence monitoring systems to enhance civil aviation safety amidst rising environmental and operational hazards. 展开更多
关键词 turbulence detection symbolic classifier quick access recorder data
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High repetition granular Co/Pt multilayers with improved perpendicular remanent magnetization for high-density magnetic recording
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作者 李智 张昆 +7 位作者 杜奥 张洪超 陈伟斌 徐宁 郝润润 颜世申 赵巍胜 冷群文 《Chinese Physics B》 SCIE EI CAS CSCD 2023年第2期405-410,共6页
Thanks to the strong perpendicular magnetic anisotropy(PMA), excellent processing compatibility as well as novel spintronic phenomenon, Co/Pt multilayers have been attracting massive attention and widely used in magne... Thanks to the strong perpendicular magnetic anisotropy(PMA), excellent processing compatibility as well as novel spintronic phenomenon, Co/Pt multilayers have been attracting massive attention and widely used in magnetic storage.However, reversed magnetic domains come into being with the increasing layer repetition ‘N’ to reduce magneto-static energy, resulting in the remarkable diminishment of the remanent magnetization(Mr). As a result, the product of Mrand thickness(i.e., the remanent moment-thickness product, Mrt), a key parameter in magnetic recording for reliable data storing and reading, also decreases dramatically. To overcome this issue, we deposit an ultra-thick granular [Co/Pt]80multilayer with a total thickness of 68 nm on granular SiNxbuffer layer. The Mrt value, Mrto saturation magnetization(Ms) ratio as well as out of plane(OOP) coercivity(Hcoop) are high up to 2.97 memu/cm^(2), 67%, and 1940 Oe(1 Oe = 79.5775 A·m^(-1)),respectively, which is remarkably improved compared with that of continuous [Co/Pt]80multilayers. That is because large amounts of grain boundaries in the granular multilayers can efficiently impede the propagation and expansion of reversed magnetic domains, which is verified by experimental investigations and micromagnetic simulation results. The simulation results also indicate that the value of Mrt, Mr/Msratio, and Hcoopcan be further improved through optimizing the granule size, which can be experimentally realized by manipulating the process parameter of SiNxbuffer layer. This work provides an alternative solution for achieving high Mrt value in ultra-thick Co/Pt multilayers, which is of unneglectable potential in applications of high-density magnetic recording. 展开更多
关键词 granular Co/Pt multilayers perpendicular magnetic anisotropy remanent moment-thickness product magnetic recording
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Recording the direct surgeon’s view with an operating microscopic view improves microscopic ophthalmic surgery training
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作者 Masayuki Akimoto Kosei Tomita +1 位作者 Miyo Yoshida Yuki Hama 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2023年第9期1555-1558,共4页
AIM:To establish a recording system with a direct view of the surgeon to supplement video recording under an operating microscope,which lacks information on the movement and position of the surgeon’s hands,and to fac... AIM:To establish a recording system with a direct view of the surgeon to supplement video recording under an operating microscope,which lacks information on the movement and position of the surgeon’s hands,and to facilitate the reproduction of a skilled surgeon’s technique by a surgeon in training.METHODS:A small camera was attached to the operating microscope with a custom adapter.Microscopic surgeon’s view and direct surgeon’s view through this new camera were recorded in the surgical recording system.Both movies were synchronized and analyzed how do surgeons handle the instruments.RESULTS:A small camera attached to the operating microscope allowed the surgeon’s hands motion to be recorded without interfering with the surgeon’s movements.Different surgeons used different methods to manipulate the ultrasound handpiece and the irrigation/aspiration device.Even in the simple paracentesis procedure,different surgeons used different methods.Surgeons-in-training were able to identify and improve their weaknesses by watching synchronized movies of their hand motions and microscopic view.CONCLUSION:Simultaneous recording the surgical field out of the operating microscopic view by a small camera set on the microscope is comprehensive and improves surgeons-in-training understanding and learning surgeries. 展开更多
关键词 operating microscopic view direct surgeon’s view video recording microscopic surgery training
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Chrysosplenium fallax Koldaeva(Chrysosplenium),a newly recorded species from China
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作者 Xiang Niyan Chen Xitang +2 位作者 Yang Tiange Liu Xihong Liu Hong 《植物科学学报》 CAS CSCD 北大核心 2024年第4期423-430,共8页
Chrysosplenium fallax Koldaeva,recently discovered and collected in Yanji,Jilin Province,repre-sents a newly recorded species of Saxifragaceae in China.This species,native to the Russian Far East,was first described a... Chrysosplenium fallax Koldaeva,recently discovered and collected in Yanji,Jilin Province,repre-sents a newly recorded species of Saxifragaceae in China.This species,native to the Russian Far East,was first described as a new species in 2021.Based on precise field investigations and specimen examination,we provide a comprehensive description of C.fallax and its seed micromorphology.Phylogenetic analysis based on chloroplast genomes of 45 Chrysosplenium species confirmed the systematic position of C.fallax in Chrysosplenium.Voucher specimens were deposited in the Herbarium of South-Central Minzu University(HSN). 展开更多
关键词 Chrysosplenium fallax Chrysosplenium Newly record PHYLOGENY China
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Three new records of lycopsids and ferns from Vietnam
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作者 LU Yu WANG Bo +2 位作者 LIN Guoliang JIANG Rihong WANG Zhenxing 《广西植物》 CAS CSCD 北大核心 2024年第10期1943-1946,共4页
Three new records from Vietnam,Phlegmariurus guangdongensis Ching,Adiantum longzhouensis A.H.Wang,F.G.Wang&F.W.Xing,and Lepisorus bicolor Ching,are reported.The identification characteristics,information of specim... Three new records from Vietnam,Phlegmariurus guangdongensis Ching,Adiantum longzhouensis A.H.Wang,F.G.Wang&F.W.Xing,and Lepisorus bicolor Ching,are reported.The identification characteristics,information of specimen,and geographical distribution of the species are described.The discovery of new records not only enriches the study of plant diversity in Vietnam,but also provides important information for biodiversity conservation and botanical research in the region.The voucher specimens are kept in the Herbarium of Kunming Institute of Botany,Chinese Academy of Sciences(KUN),Herbarium of Institute of Botany,Chinese Academy of Sciences(PE)and Herbarium of Guangxi Forestry Research Institute(GXFI). 展开更多
关键词 Phlegmariurus guangdongensis Adiantum longzhouensis Lepisorus bicolor new record VIETNAM
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Machine learning for carbonate formation drilling: Mud loss prediction using seismic attributes and mud loss records
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作者 Hui-Wen Pang Han-Qing Wang +4 位作者 Yi-Tian Xiao Yan Jin Yun-Hu Lu Yong-Dong Fan Zhen Nie 《Petroleum Science》 SCIE EI CAS CSCD 2024年第2期1241-1256,共16页
Due to the complexity and variability of carbonate formation leakage zones, lost circulation prediction and control is one of the major challenges of carbonate drilling. It raises well-control risks and production exp... Due to the complexity and variability of carbonate formation leakage zones, lost circulation prediction and control is one of the major challenges of carbonate drilling. It raises well-control risks and production expenses. This research utilizes the H oilfield as an example, employs seismic features to analyze mud loss prediction, and produces a complete set of pre-drilling mud loss prediction solutions. Firstly, 16seismic attributes are calculated based on the post-stack seismic data, and the mud loss rate per unit footage is specified. The sample set is constructed by extracting each attribute from the seismic trace surrounding 15 typical wells, with a ratio of 8:2 between the training set and the test set. With the calibration results for mud loss rate per unit footage, the nonlinear mapping relationship between seismic attributes and mud loss rate per unit size is established using the mixed density network model.Then, the influence of the number of sub-Gausses and the uncertainty coefficient on the model's prediction is evaluated. Finally, the model is used in conjunction with downhole drilling conditions to assess the risk of mud loss in various layers and along the wellbore trajectory. The study demonstrates that the mean relative errors of the model for training data and test data are 6.9% and 7.5%, respectively, and that R2is 90% and 88%, respectively, for training data and test data. The accuracy and efficacy of mud loss prediction may be greatly enhanced by combining 16 seismic attributes with the mud loss rate per unit footage and applying machine learning methods. The mud loss prediction model based on the MDN model can not only predict the mud loss rate but also objectively evaluate the prediction based on the quality of the data and the model. 展开更多
关键词 Lost circulation Risk prediction Machine learning Seismic attributes Mud loss records
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New record of the feather star Palaeocomatella hiwia(Echinodermata:Crinoidea:Comatulida)from Zhenbei Seamount in South China Sea:new insights into the taxonomic position of Palaeocomatella
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作者 Shao’e SUN Zijie MEI +2 位作者 Zhongli SHA Jing WANG Nansheng CHEN 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2024年第1期244-251,共8页
One specimen belonging to the family Comatellinae was collected from the Zhenbei Seamount(332.5–478.2 m)in the South China Sea in July 2022.Based on the morphological characters,the specimen was identified as Palaeoc... One specimen belonging to the family Comatellinae was collected from the Zhenbei Seamount(332.5–478.2 m)in the South China Sea in July 2022.Based on the morphological characters,the specimen was identified as Palaeocomatella hiwia McKnight,1977.It is first recorded from China Sea and redescribed in detail.This specimen differs from the original description from New Zealand for never showing syzygy at br4+5 or br5+6 on interior and br1+2 on exterior arms.However,it is much conform to the redescription to specimens from Indonesia,with only differences in position of the second syzygy and distalmost pinnule comb.Specimen is deposited in the Institute of Oceanology,Chinese Academy of Sciences.Phylogenetic analyses based on the mitochondrial c oxidase subunit I(COI)and 16S rRNA genes indicated that P.hiwia was nested within the tribe Phanogeniini and clustered with Aphanocomaster pulcher.Furthermore,P.hiwia showed same morphological features in terms of mouth placement,comb location,and number of comb teeth rows as other genera of Phanogeniini.Therefore,we suggest that the genus Palaeocomatella should be put in the tribe Phanogeniini. 展开更多
关键词 new record Palaeocomatella hiwia McKnight 1977 South China Sea phylogenetic relationship TAXONOMY
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Development of Self-Rated Nursing Record Frequency for Delirium Care of Nurses in Acute Care Hospitals (NRDC-Acute)
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作者 Katsuhiko Hattori Kenichi Matsuda 《Open Journal of Nursing》 2024年第8期412-420,共9页
Background: Nursing records play an important role in multidisciplinary collaborations in delirium care. This study aims to develop a self-rated nursing record frequency scale for delirium care among nurses in acute c... Background: Nursing records play an important role in multidisciplinary collaborations in delirium care. This study aims to develop a self-rated nursing record frequency scale for delirium care among nurses in acute care hospitals (NRDC-Acute). Methods: A draft of the scale was developed after a literature review and meeting with researchers with experience in delirium care, and a master’s or doctoral degree in nursing. We identified 25 items on a 5-point Likert scale. Subsequently, an anonymous self-administered questionnaire survey was administered to 520 nurses from 41 acute care hospitals in Japan, and the reliability and validity of the scale were examined. Results: There were 232 (44.6%) respondents and 218 (41.9%) valid responses. The mean duration of clinical experience was 15.2 years (SD = 8.8). Exploratory factor analysis extracted 4 factors and 13 items for this scale. The model fit indices were GFI = 0.991, AGFI = 0.986, and SRMR = 0.046. The Cronbach’s alpha coefficient for the entire scale was .888. The four factors were named “Record of Pharmacological Delirium Care on Pro Re Nata (PRN)”, “Record of Non-Pharmacological Delirium Care”, “Record of Pharmacological Delirium Care on Regular Medication”, and “Record of Collaboration for Delirium Care”. Conclusion: The scale was relatively reliable and valid. Nurses in acute care hospitals can use this scale to identify and address issues related to the documentation of nursing records for delirium care. 展开更多
关键词 Acute Care Hospitals Delirium Care Nurses Nursing records Scale Development
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Impact of Laboratory Value Flowsheet in Electronic Health Record (EHR) Documentation Time
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作者 Isabel Rosado Pogozelski 《Open Journal of Nursing》 2024年第1期40-50,共11页
Research on the use of EHR is contradictory since it presents contradicting results regarding the time spent documenting. There is research that supports the use of electronic records as a tool to speed documentation;... Research on the use of EHR is contradictory since it presents contradicting results regarding the time spent documenting. There is research that supports the use of electronic records as a tool to speed documentation;and research that found that it is time consuming. The purpose of this quantitative retrospective before-after project was to measure the impact of using the laboratory value flowsheet within the EHR on documentation time. The research question was: “Does the use of a laboratory value flowsheet in the EHR impact documentation time by primary care providers (PCPs)?” The theoretical framework utilized in this project was the Donabedian Model. The population in this research was the two PCPs in a small primary care clinic in the northwest of Puerto Rico. The sample was composed of all the encounters during the months of October 2019 and December 2019. The data was obtained through data mining and analyzed using SPSS 27. The evaluative outcome of this project is that there is a decrease in documentation time after implementation of the use of the laboratory value flowsheet in the EHR. However, patients per day increase therefore having an impact on the number of patients seen per day/week/month. The implications for clinical practice include the use of templates to improve workflow and documentation as well as decreasing documentation time while also increasing the number of patients seen per day. . 展开更多
关键词 Electronic Health record EHR Laboratory Results Template Documentation Time
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Case Records as Medical Stories: A Song-dynasty Doctor’s Narration of His Own Medicine-Xu Shuwei (1080-1154)
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作者 Asaf Goldschmidt 《Chinese Medicine and Culture》 2024年第2期95-103,共9页
The key point in studying or teaching the history of Chinese medicine is on the doctrines underlying it and on its perception of the body,physiology,pathology,and its treatment.Namely,there is often a tendency to focu... The key point in studying or teaching the history of Chinese medicine is on the doctrines underlying it and on its perception of the body,physiology,pathology,and its treatment.Namely,there is often a tendency to focus on reading and analysing the classical canons and therapy-related texts including formularies and materia medica collections.However,focusing on these sources provides us with a one-sided presentation of Chinese medicine.These primary sources lack the clinical down-to-earth know-how that encompasses medical treatment,which are represented,for instance,in the clinical rounds of modern medical schools.Our traditional focus on the medical canons and formularies provides almost no clinical knowledge,leaving us with a one-sided narrative that ignores how medicine and healing are actually practiced in the field.This paper focuses on the latter aspect of medicine from a historical perspective.Using written and visual sources dating to the Song dynasty,clinical encounters between doctors and patients including their families are depicted based on case records recorded by a physician,members of the patient’s family,and bystanders.This array of case records or case stories will enable us to narrate the interaction between physicians and patients both from the clinical perspective and from the social interaction.This paper will also discuss visual depictions of the medical encounter to provide another perspective for narrating medicine during the Song dynasty.Medical case records and paintings depicting medical encounters are exemplary of the potential of Chinese primary sources for narrative medicine. 展开更多
关键词 Clinical encounter Medical practice Song dynasty Xu Shuwei Case records
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The Development of the “Monitoring Application for Inappropriate Expressions in Nursing Records”for PsyNACS©
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作者 Hirokazu Ito Yuko Yasuhara Tetsuya Tanioka 《Journal of Computer and Communications》 2024年第5期42-52,共11页
Lengthening periods of hospitalization, increasing numbers of patients with age-related complications, and a shortage of nursing staff have been of great concern in medical psychiatry in Japan. Under these circumstanc... Lengthening periods of hospitalization, increasing numbers of patients with age-related complications, and a shortage of nursing staff have been of great concern in medical psychiatry in Japan. Under these circumstances, countries such as Japan that face a super-aging society and a decline in the working-age population, have been recommended for use of advanced information and communications technology (ICT) to improve the efficiency of medical treatment and care. This study aims to develop the “Monitoring Application for Inappropriate Expressions in Nursing Records” for using PsyNACS<sup>©</sup>, which will enable psychiatric nursing plans to harness the advantages of ICT. The functions considered necessary are as follows: 1) identification of users who enter information;2) a necessary database for lists of inappropriate expressions;3) development of a matching function to recommend proper writing input and a warning function;and 4) a management function for an inappropriate expression list database. A demonstration experiment for developing the application in this study was conducted at a specialized psychiatric hospital. To introduce them to the application, nurses and nurse managers were informed about the system developed in this study, and a survey regarding their opinions on the functions of the application was conducted with ten nurse managers. The results were evaluated in terms of usefulness for nursing care, documentation, and the education of nurses from an ethical perspective. This study suggests that matching their input with an inappropriate expression database will allow nurses to record more appropriate expressions. From an ethical perspective, the ability to use appropriate expressions makes records more likely to withstand disclosure requests from patients and their families. 展开更多
关键词 PsyNACS© Monitoring Application Inappropriate Expressions Nursing records
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解铃须从系铃处——Records与Archives概念正史(二)
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作者 王岚 《档案管理》 北大核心 2024年第1期28-35,共8页
笔者的“四正”,从名分、本义、学理、法律上已弄清Records的含义,但坚持“Records文件观”者依旧质疑:若Records是档案,那Archives是什么?两者并列时又怎么翻译?还有人说Records是记录。既然是英语和法语带来的问题,循着英法档案历史... 笔者的“四正”,从名分、本义、学理、法律上已弄清Records的含义,但坚持“Records文件观”者依旧质疑:若Records是档案,那Archives是什么?两者并列时又怎么翻译?还有人说Records是记录。既然是英语和法语带来的问题,循着英法档案历史去寻找,才是解铃的关键:以英法八百年档案工作中重要人物和事件为主线,探求其档案的源流、本质、内含及演变,为档案概念正史。 展开更多
关键词 卷档 录卷归档 档案 皇家档案 馆藏档案 现行档案 概念 历史
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解铃须从系铃处——Records与Archives概念正史(三)
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作者 王岚 《档案管理》 北大核心 2024年第2期27-33,共7页
笔者的“四正”,从名分、本义、学理、法律上已弄清Records的含义,但坚持“Records文件观”者依旧质疑:若Records是档案,那Archives是什么?两者并列时又怎么翻译?还有人说Records是记录。既然是英语和法语带来的问题,循着英法档案历史... 笔者的“四正”,从名分、本义、学理、法律上已弄清Records的含义,但坚持“Records文件观”者依旧质疑:若Records是档案,那Archives是什么?两者并列时又怎么翻译?还有人说Records是记录。既然是英语和法语带来的问题,循着英法档案历史去寻找,才是解铃的关键:以英法八百年档案工作中重要人物和事件为主线,探求其档案的源流、本质、内含及演变,为档案概念正史。 展开更多
关键词 卷档 录卷归档 档案 皇家档案 馆藏档案 现行档案 概念 历史
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Isolation of Protoplast and Ion Channel Recording in Plasma Membrane of Suspension Cells of Populus euphratica 被引量:4
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作者 陈少良 戴松香 +3 位作者 李金克 王沙生 Andrea Polle Aloys Hüttermann 《Forestry Studies in China》 CAS 2002年第1期1-4,共4页
The authors used suspension cells of Populus euphratica to isolate protoplast in the present study. Protoplasts were successfully obtained after 4 hours incubation in enzyme solution containing 1 0% cellulase “o... The authors used suspension cells of Populus euphratica to isolate protoplast in the present study. Protoplasts were successfully obtained after 4 hours incubation in enzyme solution containing 1 0% cellulase “onozuka” R\|10, 0\^01% pectolyase Y\|23,0\^15% macerozyme R\|10 and 0\^1% hemicellulase at 25℃. Outward and inward single channels in plasma membrane were observed using cell\|attached recording of patch\|clamp technique. In this study, single channel records showed that more than one species of channel were obtained. These attempts in protoplast isolation and ion channel recording offers the opportunity to characterize cellular mechanisms of salt tolerance in tree species. 展开更多
关键词 Populus euphratica suspension cells PROTOPLASTS patch\|clamp cell\|attached recording technique single ion channel
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FAULT LOCATION AND RECORDING SYSTEM OF TRANSMISSION LINES BASED ON COMPUTER NETWORK 被引量:1
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作者 贺家李 孙雅明 贺继红 《Transactions of Tianjin University》 EI CAS 1997年第1期17-23,共7页
In this paper a fault location and recording system based on a computer network is presented. A brief description of the system structure and main features are given. Emphasis is placed on the accurate fault location ... In this paper a fault location and recording system based on a computer network is presented. A brief description of the system structure and main features are given. Emphasis is placed on the accurate fault location method for extra high voltage and long distance transmission lines. 展开更多
关键词 computer network fault location recording system
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Microelectrode array for bioelectrical signal stimulation and recording
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作者 潘海仙 吕晓迎 +3 位作者 王志功 方涛 邱雷 黄宗浩 《Journal of Southeast University(English Edition)》 EI CAS 2011年第4期361-366,共6页
A microelectrode array(MEA) is presented, which is composed of 60 independent electrodes with 59 working ones and one reference one, and they are divided into 30 pairs. Except for the reference electrode, each pair ... A microelectrode array(MEA) is presented, which is composed of 60 independent electrodes with 59 working ones and one reference one, and they are divided into 30 pairs. Except for the reference electrode, each pair consists of one stimulating electrode and one recording electrode. Supported by the peripheral circuits, four electrode states to study the bioelectrical signal of biological tissue or slice cultured in-vitro on the surface of the electrodes can be realized through each pair of electrodes. The four electrode states are stimulation, recording, stimulation and recording simultaneously, and isolation. The state of each pair of working electrodes can be arbitrarily controlled according to actual needs. The MEAs are fabricated in printed circuit board (PCB) technology. The total area of the PCB-based MEA is 49 mm × 49 mm. The impedance measurement of MEA is carried out in 0.9% sodium chloride solution at room temperature by means of 2-point measurements with an Agilent LCR meter, and the test signal for the impedance measurement is sinusoidal (AC voltage 50 mV, sweeping frequency 20 Hz to 10 kHz). The electrode impedance is between 200 and 3 kΩ while the frequency is between 500 and 1 000 Hz. The electrode impedance magnitude is inversely proportional to the frequency. Experiments of toad sciatic nerve in-vitro stimulation and recording and signal regeneration between isolated toad sciatic nerves are carried out on the PCB-based MEA. The results show that the MEA can be used for bioelectrical signal stimulation, recording, stimulation and recording simultaneously, and isolation of biological tissues or slices in-vitro. 展开更多
关键词 microelectrode array (MEA) stimulation and recording extracellular recording toad sciatic nerve
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An optimized recording method to characterize biophysical and pharmacological properties of acid-sensing ion channel
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作者 李爱 司文 +2 位作者 胡新武 刘长金 曹晓华 《Neuroscience Bulletin》 SCIE CAS CSCD 2008年第3期160-165,共6页
Objective To re-confirm and characterize the biophysical and pharmacological properties of endogenously expressed human acid-sensing ion channel 1a (hASIC1a) current in HEK293 cells with a modified perfusion methods... Objective To re-confirm and characterize the biophysical and pharmacological properties of endogenously expressed human acid-sensing ion channel 1a (hASIC1a) current in HEK293 cells with a modified perfusion methods. Methods With cell floating method, which is separating the cultured cell from coverslip and putting the cell in front of perfusion tubing, whole cell patch clamp technique was used to record hASICla currents evoked by low pH external solution. Results Using cell floating method, the amplitude of hASICla currents activated by pH 5.0 in HEK293 cells is twice as large as that by the conventional method where the cells remain attached to coverslip. The time to reach peak at two different recording conditions is (21±5) ms and (270±25) ms, respectively. Inactivation time constants are (496±23) ms and (2284±120) ms, respectively. The cell floating method significantly increases the amiloride potency of block on hASIC 1 a [IC50 is (3.4± 1.1 ) μmol/L and (2.4± 0.9) μmol/L, respectively]. Both recording methods have similar pH activation ECs0 (6.6±0.6, 6.6±0.7, respectively). Conclusion ASICs channel activation requires fast exchange of extracellular solution with the different pH values. With cell floating method, the presence of hASIC la current was re-confirmed and the biophysical and pharmacological properties of hASIC la channel in HEK293 cells was precisely characterized. This method could be used to study all ASICs and other ligand-gated channels that require fast extracellular solution exchange. 展开更多
关键词 acid-sensing ion channel patch-clamp recording PH
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Strong motion observations and recordings from the great Wenchuan Earthquake 被引量:71
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作者 Li Xiaojun Zhou Zhenghua +5 位作者 Yu Haiyin Wen Ruizhi Lu Dawei Huang Moh Zhou Yongnian Cu Jianwen 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2008年第3期235-246,共12页
The National Strong Motion Observation Network System (NSMONS) of China is briefly introduced in this paper. The NSMONS consists of permanent free-field stations, special observation arrays, mobile observatories and... The National Strong Motion Observation Network System (NSMONS) of China is briefly introduced in this paper. The NSMONS consists of permanent free-field stations, special observation arrays, mobile observatories and a network management system. During the Wenchuan Earthquake, over 1,400 components of acceleration records were obtained from 460 permanent free-field stations and three arrays for topographical effect and structural response observation in the network system from the main shock, and over 20,000 components of acceleration records from strong aftershocks occurred before August 1, 2008 were also obtained by permanent free-field stations of the NSMONS and 59 mobile instruments quickly deployed after the main shock. The strong motion recordings from the main shock and strong aftershocks are summarized in this paper. In the ground motion recordings, there are over 560 components with peak ground acceleration (PGA) over 10 Gal, the largest being 957.7 Gal. The largest PGA recorded during the aftershock exceeds 300 Gal. 展开更多
关键词 Wenchuan Earthquake strong motion observation network mobile observation observation array strong motion record PGA
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