普通菜豆(Phaseolus vulgaris L.)AOX基因家族鉴定与表达分析

交替氧化酶(Alternative oxidase,AOX)是一种末端氧化酶,在维持植物正常生长代谢速率和代谢平衡以及抵御逆境胁迫中发挥了一定作用。AOX家族成员已经在很多植物物种中被鉴定,但是在普通菜豆(Phaseolus vulgaris L.)中却未见报道。研究对普通菜豆中AOX家族成员进行了鉴定和分析,并进一步解析了AOX基因家族成员的功能及调控机制。结果表明,基于普通菜豆参考基因组中共挖掘得到了4个AOX家族成员,通过进化关系、保守基序(Motifs)、基因结构分析,将各成员划分成4个亚族。此外,顺式作用元件、共线性与盐胁迫下的表达量分析结果表明,AOX成员可能在植物的非生物胁迫响应中发挥了作用。为后续深入研究普通菜豆中AOX成员的功能奠定了理论基础。

A novel pathogen Fusarium cuneirostrum causing common bean(Phaseolus vulgaris)root rot in China

Several fungal pathogens cause root rot of common bean,among which Fusarium spp.are the most common pathogens causing Fusarium root rot(FRR)worldwide.FRR has been becoming an increasingly severe disease of common bean in China,but the species of Fusarium spp.have remained unclear.Thus,this study was performed to identify the pathogen causing common bean root rot in Liangcheng County,Inner Mongolia,China.Nineteen Fusarium-like isolates were obtained after pathogen isolation and purification.The pathogenicity test indicated that eight isolates caused severe disease symptoms on common bean,while 11 other isolates were not pathogenic.The eight pathogenic isolates,FCL1–FCL8,were identified as Fusarium cuneirostrum by morphological characterization and phylogenetic analysis using partial sequences of EF-1α,ITS,28S,and IGS regions.Host range test showed that the representative F.cuneirostrum isolate FCL3 was also pathogenic to mung bean,while not pathogenic to adzuki bean,chickpea,cowpea,faba bean,pea,and soybean.Moreover,50 common bean and 50 mung bean cultivars were screened for resistance to FRR,and seven highly resistant or resistant cultivars of common bean were identified,while no resistant cultivars of mung bean were screened.This study revealed that F.cuneirostrum was one of common bean FRR pathogens in Inner Mongolia and it could induce mung bean root rot as well.To our knowledge,this is the first report of F.cuneirostrum causing FRR of common bean in China.

Common Organic Amendment (Rice Straw) Can Reduce Salinity Effects on Bean (Phaseolus vulgaris) Growth with or without Photoperiod Manipulation

Soil salinity is a major limiting factor for crop production in coastal areas of Bangladesh. Cheap and sustainable management of soil salinity is hence most sought out topics in agricultural research. Conceptualizing that idea in mind, a pot experiment was conducted in the Department of Soil, Water & Environment, University of Dhaka in order to analyze if common organic amendments (rice straw, saw dust) coupled with reduce photoperiod can mitigate salinity effect on the growth of bean (Phaseolus vulgaris). The experiment was set up following completely randomized design (CRD) with nine treatments and three replications containing Tc (Control), T1 (Ambient photoperiod + 110 mM Salinity treatment + Rice straw), T2 (Reduced photoperiod + 110 mM Salinity treatment + Rice straw), T3 (Ambient photoperiod + 220 mM Salinity treatment + Rice straw), T4 (Reduced photoperiod + 220 mM Salinity treatment + Rice straw), T5 (Ambient photoperiod + 110 mM Salinity treatment + Saw dust), T6 (Reduced photoperiod + 110 mM Salinity treatment + Saw dust), T7 (Ambient photoperiod + 220 mM Salinity treatment + Saw dust) and T8 (Reduced photoperiod + 220 mM Salinity treatment + Saw dust). Organic amendments were used separately at the rate of 12 ton/ha. The highest plant height (98.67 cm), root length (12.5 cm), pod number (10.33), leaf area (13.99 cm2), fresh weight (680 kg/ha), dry weight (316.67 kg/ha) were recorded with the treatment T1 while the second-best treatment was treatment T2 (with highest harvest index 0.040) and these results were statistically significant (p < 0.001). In post-harvest soil, pH, EC, OC, OM;available N, P, K, S;total Ca, Mg, Zn, Mn were increased significantly in treatment T1. The overall results illustrated that the best growth and yield performances were achieved in the treatment T1 and T2.

Effect of Biostimulants Based on Natural Products on the Growth and Nutritional Value of Bean (Phaseolus vulgaris L.)

Beans (Phaseolus vulgaris L.) are widely grown in Cameroon and play a key role in the fight against food insecurity, malnutrition and poverty. However, its cultivation encounters problems due to abiotic and biotic stresses, which leads to the use of synthetic fertilizers and pesticides, which cause significant damage to the environment and human health due to the presence of synthetics residues in the seeds, pods and in the leaves that are eaten. Promoting the use of natural products is becoming a necessity for organic and eco-responsible agriculture that limits contamination problems and improves people’s purchasing power. This study aims to assess the effect of biostimulants based on natural products on the growth and nutritional value of common bean (Phaseolus vulgaris L.). Bean seedlings from white variety (MEX-142) and red variety (DOR-701) were treated every seven days in the field from their pre-emergence, emergence and growth to their maturation under a randomized block experimental design. Six treatments and three repetitions with the biostimulants based on natural products and controls were thus performed and the agromorphological parameters were measured. After 120 days, the contents of growth biomarkers and defense-related enzymes were evaluated in leaves, while the contents of macromolecules, minerals and antinutrients were evaluated in seeds. These biostimulants significantly increased (P P < 0.0001) of antinutrients including oxalates, phytates, tannins and saponins in seeds compared to controls (T+ and T−). Treatment with biostimulants, in particular BS4, improves the performance of bean plants in the field as well as the biofortification of seeds regardless of the variety.

Transcriptome Analysis of Molecular Mechanisms Underlying Phenotypic Variation in Phaseolus vulgaris Mutant‘nts’

The phenotype of a common bean plant is often closely related to its yield,and the yield of plants with reduced height or poor stem development during growth is low.Mutants serve as an essential gene resource for common bean breeding genetic research.Although model plants and crops are studied to comprehend the molecular mechanisms and genetic basis of plant phenotypes,the molecular mechanism of phenotypic variation in common beans remains underexplored.We here used the mutant‘nts’as material for transcriptome sequencing analysis.This mutant was obtained through 60Co-γirradiation from the common bean variety‘A18’.Differentially expressed genes were mainly enriched in GO functional entries such as cell wall organization,auxin response and transcription factor activity.Metabolic pathways significantly enriched in KEGG analysis included plant hormone signal transduction pathways,phenylpropanoid biosynthesis pathways,and fructose and mannose metabolic pathways.AUX1(Phvul.001G241500),the gene responsible for auxin transport,may be the key gene for auxin content inhibition.In the plant hormone signal transduction pathway,AUX1 expression was downregulated and auxin transport across the membrane was blocked,resulting in stunted growth of the mutant‘nts’.The results provide important clues for revealing the molecular mechanism of‘nts’phenotype regulation in bean mutants and offer basic materials for breeding beneficial phenotypes of bean varieties.

Insight into Genetic Diversity of Cultivated Lima Bean (Phaseolus lunatus L.) in Benin

Lima bean is a tropical and subtropical legume from the genus Phaseolus which is cultivated for its importance in food and in medicine, but which remains a Neglected and Underutilized Crop in Benin. Understanding the genetic diversity of a species’ genetic resources is useful for the establishment of appropriate conservation strategies and breeding programs and for sustainable use. We use 6 out of ten SSR markers to analyze the diversity and population structure of 28 Lima bean landraces collected in Benin. A total of 28 alleles with an average of 4.16 alleles per SSR were amplified. The Polymorphic Information Content value ranged from 0.079 to 0.680 with an average of 0.408. The analysis of population structure revealed three subpopulations. PCoA revealed three well-separated clusters among the analyzed accessions in accordance with the population structure results and the clustering based on the Neighbor-Joining tree. AMOVA showed highly significant (p = 0.001) diversity among and within populations. Hence, 32% of the genetic variation was distributed among the population and 68% was distributed within populations. A high PhiP value (0.321) was found between the three sub-subpopulations indicating a high genetic differentiation between these sub-subpopulations. By exhibiting the highest average number of alleles, Shannon-Weaver information and Shannon-Weaver diversity indices, and the highest mean number of private alleles, subpopulation 1 is the main gene pool of the analyzed collection. The present study is an important starting point for the establishment of appropriate conservation strategies and breeding programs for Lima bean genetic resources.

Comparative Experiments on Different Varieties of Phaseolus vulgaris L. under Dry Farming Conditions

[Objective] The aim was to screen varieties of Phaseolus vulgaris L.for cultivation in arid,semi-arid areas.[Method] The cultivation experiments of seven varieties of P.vulgaris under dry farming conditions were carried out in typical dry farming agriculture region Yangqu County in North China.[Result] The plants of Teyoutejiadou,Chaojiwujing and Xinxinyichiqingyuanlong were relatively strong;the growth rate of Aifeng,Jingxuanjiadouwang and Chaochangsijidou was relatively rapid;the transpiration rate of functional leaves of Aifeng was the lowest.The yield of Tianma 95-33 jiadouwang was significant higher than that of other varieties,the unit yield was up to 36.33 t/hm2,which was 1.39 times higher than that of Aifeng and 1.9 times higher than that of Xinxinyichiqingyuanlong;followed by the yield of Aifeng（26.07 t/hm2）,which was 1.37 times higher than that of Xinxinyichiqingyuanlong.[Conclusion] The yield of Tianma 95-33 jiadouwang and Aifeng was relatively high,as well as the protein content,indicating that they were suitable to be applied and cultivated in the experimental plot and surrounding areas.

Leaf area development, dry weight accumulation and solar energy conversion efficiencies of Phaseolus vulgaris L. under different soil moisture levels near Nairobi, Kenya

Leaf area development, dry weight accumulation and solar energy conversion efficiencies of Phaseolus vulgaris L. cv GLP\|2 under two soil moisture levels in two contrasting seasons near Nairobi, Kenya were investigated. The experiment confirms that dry weights and yields of Phaseolus vulgaris are limited by a drought induced decrease in leaf area, leading to less radiation interception as a source for assimilation. However, photosynthetic efficiency in Phaseolus vulgaris also appears to decrease and to contribute to these effects. Finally, an even larger decreases of economic efficiency as obtained in the second season, where stress lasted much later into the season, reveals that such a drought also limits considerably the partitioning and translocation of assimilates to the seeds of Phaseolus vulgaris. The efficiencies obtained are in line with the better literature data for other crops.

Hydrogen Peroxide Alleviates Hypoxia during Imbibition and Germination of Bean Seeds (<i>Phaseolus vulgaris</i>L.)

Bean seeds (Phaseolus vulgaris L. cv. Tendergreen) were imbibed in water to examine the effects of hypoxia during imbibition and subsequent germination. Hypoxic conditions occurred when seeds were imbibed in water for 24 h or longer and resulted in severe reduction of hypocotyl elongation and stem growth during subsequent germination under non-limiting oxygen conditions. Under continued hypoxic conditions, bean seeds failed to germinate, however, this was reversed in the presence of hydrogen peroxide (20 mM) in the medium. Furthermore, imbibition of seeds in the presence of hydrogen peroxide overcame the adverse hypoxic effects on hypocotyl elongation and stem growth. Exogenous hydrogen peroxide increased the dissolved oxygen levels in the germinating medium, and catalase and transition metal ions such as Fe2+, Cu2+ and Mn2 helped to facilitate the production of oxygen from hydrogen peroxide. In these catalysts, catalase played a major role in the decomposition of hydrogen peroxide as demonstrated by the use of a catalase inhibitor, 3-amino-1, 2, 4-triazole, which reversed the positive effects produced by hydrogen peroxide on germination of seeds under hypoxic conditions. The results show that imbibition is sensitive to oxygen deficits which affect subsequent hypocotyl growth and seedling performance. The adverse effects of hypoxia on germination of bean seeds can be overcome by exogenous hydrogen peroxide.

The effect of external Ca^(2+) and Ca^(2+)-channel modulators on red-light-induced swelling of protoplasts of Phaseolus radiatus L.

Red-light-induced swelling of the protoplasts isolated from hypocotyl of etiolated mung bean (Phaseolus radiatus L.) was observed only when Ca2+ ions were present in the medium. The optimal CaCl2 concentration was 250 μM. Swelling response declined when Ca2+ was supplied into the medium after red light irradiation. The Ca2+-chelator EGTA eliminated the red-light-induced swelling and 45Ca2+ accumulation in the protoplasts. In contrast, A23187, a Ca2+-ionophore, could mimic the effect of red light in darkness. These results indicate that Ca2+ may play a role in light signal transduction. In addition, swelling response was prevented by TFP and CPZ (both are CaM antagonists), implying the involvement of CaM in red-light-induced and Ca2+ -dependent protoplast swelling.

Effects of zinc and nitrogen fertilizer and their application method on yield and yield components of <i>Phaseolus vulgaris</i>L.

An experiment in factorial format based on randomized complete block design with 3 replications was conducted to study the effects of zinc spray (0 and 1 g/L) and nitrogen fertilizer (0, 25, 50 and 75 kg/ha pure nitrogen) on yield and yield components of Phaseolus vulgaris. In maturity time, seed yield, 100 seed weight, number of pods per plant, number of seeds per pod and plant height were measured. Results showed that, use of zinc spray had a significant effect in 1% probability level on all measured traits. Also, the effect of nitrogen on all studied traits was significant in 1% probability level. Interaction effect of zinc spray and nitrogen fertilizer on number of seed per pod in 1% and on seed yield and plant height in 5% was significant and on other traits was non significant. The highest seed yield was obtained by zinc spray application with 1996 kg/ha. Among nitrogen fertilizer levels, use of 90 kg/ha pure nitrogen showed highest seed yield.

Effects of Different Ridge Culture Ways on Stem Diameter,Plant Height,the Number of Leaves and Yield of Phaseolus vulgaris L. under Dry Farming Conditions

The variety "Aifeng" was used as experimental material,and the stem diameter,plant height,the number of leaves and yield of P. vulgaris under three ways of ridge culture (bedding,high ridge and M ridge) were observed and detected to study the effects of different ridge cultures on the growth and yield. The results showed that the stem diameter,plant height,the number of leaves and yield under M ridge culture were higher than that of bedding and high ridge.

Improvement of Phaseolus vulgaris on breastfeeding in female rats

Objective: To evaluate effect of Phaseolus vulgaris (P. vulgaris) on the breastfeeding in female rats. Methods: This experimental study was done from May 2018 to December 2018 in the Anatomical Department of Medical School in Kermanshah University of Medical Sciences in Iran. In this study, after one-week adaptation and fertilization by male, 40 female rats within 20 days of pregnancy (on average, every mother had 10 newborns) were equally separated into four groups (animals were administrated after delivery of offspring). Group 1 was control group receiving normal saline interaperitoneally, and groups 2, 3, 4 were treatment groups receiving the dose of 20, 50, 100 mg/kg of P. vulgaris interaperitoneally respectively once a day for 60 days. The prolactin hormone was measured by radio immune assay, number and diameter of alveoli via histological and morphometrical examinations, and receptor prolactin gene expression by real-time polymerase chain reaction method. Results: P. vulgaris significantly improved alveoli's number and diameter, prolactin hormone and receptor prolactin expression when compared to the control group (P<0.001). Conclusions: P. vulgaris is helpful to improve the breastfeeding parameters of rats' mammary glands.

Chemical and Functional Properties of Hard-to-Cook Bean (<i>Phaseolus vulgaris</i>) Protein Concentrate

The objective of this research was to evaluate the chemical and functional properties of hard-to-cook (HTC) bean (Phaseolus vulgaris) protein concentrate to determine their potential practical applications. The respective protein concentrate was obtained from the flour using isoelectric precipitation and the protein content was 73.03%. Proximate composition and in vitro digestibility were measured to evaluate the chemical properties, and nitrogen solubility, emulsifying capacity, emulsion stability, foaming capacity, foam stability and viscosity were measured to evaluate its functional properties. The proximate composition of the HTC bean (P. vulgaris) flour and protein concentrate registered values of moisture, ash, protein, fat, fiber and NFE of 8.92, 4.52, 21.71%, 4.41%, 4.11% and 65.25% for flour and of 2.68%, 2.54%, 73.03%, 2.77%, 1.31% and 20.35% for protein concentrate. The in vitro digestibility was of 76.7%. The hard-to-cook bean protein concentrate exhibited good functional properties suggesting its use as additive. This concentrate registered solubility values that are ranging from 2.5% to 71.81%. The emulsifying (EC) and foaming capacity (FC) registered values of 89% - 97% and of 7% - 53% at different pH levels, respectively as well as an emulsion (ES) and foaming stability (FS) pH- and time-dependent. The HTC bean (P. vulgaris) protein concentrate registered a viscosity profile dependent of shear rate. The results suggest that HTC bean (P. vulgaris) protein concentrate is a valuable food ingredient or additive.

Ultrastructural Analysis of the Ontogenetic Development of Shoot Induced from Embryonic Axes of Costa Rican Bean Varieties (<i>Phaseolus vulgaris</i>L.) under <i>in Vitro</i>Conditions by Scanning Electronic Microscopy

Common bean (Phaseolus vulgaris L.) is an economic important crop and one of the major grain legumes for human consumption in Latin America, Africa and Asia. A morphological study of shoot induced from embryonic axes development in four Costa Rican bean varieties (Brunca, Huetar, Guaymi and Bribri) cultivated on MS media with or without 5 mg·L–1 de N6-benzylaminopurine (BAP) by scanning electron microscopy (SEM) was developed in the present work. Micrographs showed similarities and differences in the ultrastructure of the apical dome, epidermal surface, stomata and different types of trichomes in the varieties cultivated on organogenesis media. Genotypes with advantageous morphological characteristics for genetic transformation, in particular an exposed apical dome, were identified. This work will contribute to the optimization of the in vitro regeneration of four common bean varieties.

Esterases from the Seeds of an Edible Legume, <i>Phaseolus vulgaris</i>L.;Variability and Stability during Germination

The total protein and esterase were isolated from the seeds of Red Kidney beans (Phaseolus vulgaris). The crude protein content was observed as 15%. The germination of seeds of red kidney bean has been carried out and change in the total protein content and esterase activity was monitored. The protein content was decreased from 15% (24 hours) to 8% (144 hours) during germination. The socked seeds and seedlings of all the days of germination exhibited esterase activity. Maximum ester hydrolyzing activity was observed on 6th day of germination whereas as lowest ester hydrolyzing activity was observed 2nd day germination. Native PAGE was carried out and esterase banding pattern for two different artificial substrates was studied. The esterase banding pattern in presence of 1-Napthyl acetate showed the presence of 4 esterolytic bands while 2 esterolytic bands were observed in presence of 2-Napthyl acetate.

Response of Eight Market Classes of Dry Bean (<i>Phaseolus vulgaris</i>L.) to Pendimethalin

There is little information on the tolerance of dry bean to pendimethalin. Field studies were conducted in 2007 to 2009 at Exeter, Ontario and in 2008 and 2009 at Ridgetown, Ontario to evaluate tolerance of black, cranberry, kidney, otebo, pink, pinto, Small Red Mexican and white bean to the pendimethalin applied preplant incorporated at 1080 and 2160 g.a.i.ha-1. Pendimethalin PPI caused minimal injury in most market classes of dry bean at 1 and 2 WAE. There was no injury in various market classes of dry bean with the low dose at 1 and 2 weeks after emergence (WAE). However, at the high dose there was 0 to 4% injury at 1 WAE and 0 to 7% injury at 2 WAE in black, cranberry, kidney, otebo, pink, pinto, SRM and white bean. Pendimethalin PPI was more injurious in white bean than in black, cranberry, kidney, otebo, pink, pinto and SRM bean. Pink and SRM bean exhibited the most tolerance to pendimethalin applied PPI at 1080 g.ai.ha-1 or 2160 g.ai.ha-1. Pendimethalin caused no adverse effect on plant height, shoot dry weight, seed moisture content and seed yield of black, cranberry, kidney, otebo, pink, pinto, SRM and white bean. Based on these results, there is an adequate margin of crop safety for pendimethalin applied PPI at the proposed dose of 1080 g.ai.ha-1 in black, cranberry, kidney, otebo, pink, pinto, SRM and white bean in Ontario.

Plant Growth Response of Eight Andean Dry Bean (<i>Phaseolus vulgaris </i>L.) Genotypes to Phosphorus Fertilizer in the Greenhouse

Common bean (Phaseolus vulgaris L.) is an important legume in the tropics, with production limited by low availability of soil phosphorus (P). An experiment was conducted in the glasshouse to evaluate P use efficiency of eight dry bean genotypes (G122, Montcalm, Taylor Horticulture, Cardinal, Bukoba, Kijivu, Rojo and CAL 143) of Andean origin. The treatments included: no P (0 kg P ha-1), normal P (50 kg P ha-1), and high P (100 kg P ha-1). There was variation for the measured traits shoot biomass (g), shoot P (mg kg-1), root P (mg kg-1), seed P (mg kg-1) and seed yield (g) among genotypes and P treatments. Biomass and all P concentrations increased with increasing P level and the genotypes Kijivu, Bukoba, Montcalm and Taylor Horticulture had higher P concentrations than Rojo, G122, Cardinal and CAL 143 across treatments. Genotype × treatment interactions were observed for shoot biomass. For the no P treatment, shoot and root biomass were positively correlated with PUE (phosphorus use efficiency). PUE had higher values and varied more among genotypes in the no P treatment compared to the normal P and high P treatments. The results suggest that seed yield in dry bean can be improved by selecting for genotypes with higher PUE under limiting P. The genotypes Bukoba, Kijivu and Montcalm with the highest values for PUE under no P treatment may be exhibiting some level of tolerance to low soil phosphorus. Higher shoot weight may provide simple criteria for selecting genotypes with greater yield and PUE (phosphorus use efficiency) under limiting P conditions. Therefore, a genotype is desired that can efficiently uptake and utilize available P under limited availability of this nutrient.

Runner Bean （Phaseolus coccineus L.） Production in Chile

Runner bean is usually grown in Chile as household plantings, in small surfaces with trellis support. Several studies were made to grow runner beans extensively without trellis support, like common beans are grown. Three runner bean ecotypes were evaluated, with and without trellis support. Although the highest yield was obtained with trellis support, significant yields were obtained without trellis. Thus, this specie can be established without support like the common bean, allowing a great economic advantage especially at large scale plantings. The ecotype named ＂Quilapallar＂ was selected by its white and large grains and excellent cooking quality. Then, seed rates and dry grain yield were compared with commercial varieties of common bean. There was not statistical difference in yield among row distances, except when this was 25 cm apart. Besides, there was not statistical difference among Quilapallar and Torcaza INIA, although the later is a common bean variety with a high yield potential.

On-farm use of legume (<i>Phaseolus calcaratus</i>) and Ruzi grass on rumen fermentation and milk production in lactating dairy cows

The experiment was conducted on 10 small-holder dairy farmers under the administration of Dairy Promotion Organization of Thailand (DPO) in the Northeast region of Thailand. The aim of the research was to investigate the effect of legume (Phaseolus calcaratus, PC) as roughage mixed with Ruzi grass for dairy cows. Fours cows per farm were assigned into 2 groups including Ruzi grass (control) and PC mixed with ruzi grass (at the ratio 50:50), were given ad libitum as a roughage sources. All cows were offered a concentrate with ratio to milk yield of 1:2. The results revealed that roughage source did not affect on ruminal pH and temperature, ammonia nitrogen, blood urea nitrogen, and milk urea nitrogen concentrations. Total volatile fatty acid, acetic acid, propionic acid, and butyric acid proportions, and acetic acid to propionic acid ratio were not significantly different among treatments. Dry matter intake and digestibility of nutrient including dry matter, organic matter, crude protein, neutral detergent fiber and acid detergent fiber were not significantly different among treatments. Moreover, amount of digested dry mater was similar among treatments. However, cows received PC mixed with Ruzi grass tended to be higher on dry matter, organic matter, and crude protein digestibility and dry matter digestible nutrient intake than control group. Milk yield, and 3.5% fat corrected milk were significantly higher (P < 0.05) in cow fed with PC mixed Ruzi grass than those the control group. Milk compositions and feed cost were similar among treatments;in contrast, the income and profit from milk selling were greater in cows fed with PC mixed with Ruzi grass than the control. High quality roughage such as PC mixed with Ruzi grass could be advantages for dairy farming in the Northeast of Thailand.