After insufflating extended endophytes phomopsis B3 ferment diluent on seedling bed of rice, the effect of microbial inoculum on rice growth was studied. The investigation result of seedlings before transplanting seed...After insufflating extended endophytes phomopsis B3 ferment diluent on seedling bed of rice, the effect of microbial inoculum on rice growth was studied. The investigation result of seedlings before transplanting seedling showed that leaf age,the number of green leaves,the number of tiller of individual plant and the weight of per 100 fresh plants in microbial inoculum treatment group increased 0.11,0.12 ,0. 03 and 2.6 g respectively compared with those of control group. 20 days after transplanting, increased tiller number in microbial inoculum treatment group was 55 500 hm^2 more than that in control group. 30 days after transplanting, tiller number in microbial inoculum treatment group was 42 000 hm^2 more than that in control group. In harvest season, the yield ,weight of I 0130 grain and seed-setting rate increased 300 kg/hm2, 0. 7 g and 0. 23% respectively compared with these in control group. The result of preliminary study demonstrated that microbial inoculum confected by endophytes phomopsis B3 could increase the number of strong seedling and yield.展开更多
从云南美登木内生真菌Phomopsis species Lz42的琼脂平板发酵物中分离得到2个倍半萜和5个麦角甾醇类化合物(1～7),其中化合物1和2为新化合物.应用波谱技术确定其结构为4-Deacetyl-10-oxo-di-hydrobotrydial(1)和麦角甾-6,22-二烯-5α,8...从云南美登木内生真菌Phomopsis species Lz42的琼脂平板发酵物中分离得到2个倍半萜和5个麦角甾醇类化合物(1～7),其中化合物1和2为新化合物.应用波谱技术确定其结构为4-Deacetyl-10-oxo-di-hydrobotrydial(1)和麦角甾-6,22-二烯-5α,8α-环二氧-3-甲酸酯(2).展开更多
Fungal pathogen of asparagus stem blight was isolated. No significant genetic difference was detected among the three strains with 492 bp long ITS1-5.8S-ITS2 sequence. It was then identified through colony growth, con...Fungal pathogen of asparagus stem blight was isolated. No significant genetic difference was detected among the three strains with 492 bp long ITS1-5.8S-ITS2 sequence. It was then identified through colony growth, conidia morphology, and molecular characterization. The physiological response to oxidation and osmosis stress, and virulence to Asparagus officinalis L. were analyzed. The results showed that the pathogen causing asparagus stem blight for A. officinalis L. in Jiangxi Province is Phomopsis asparagri (Sacc.) Bubák. Under pure culture conditions, the conidia were oval-shaped (α-type), with colorless single spore and single nucleus, containing 0-2 oil balls. Its vegetative growth rate was higher when cultured on 0.2 × potato dextrose agar (0.2 × PDA) medium than that on oatmeal agar (OA) medium. However, the pycnidia appeared earlier on OA medium than on 0.2 earlier PDA medium. The vegetative growth rate was depressed under oxidation (H2O2) or osmosis (NaCl) stress conditions, and totally inhibited under 7 mmol/L H2O2 or 2.4 mol/L NaCl. All the strains caused typical pathogenic symptoms to Asparagus officinalis L. at 7 days-post-inoculation (dpi) with conidia.展开更多
A severe leaf spot disease on patchouli,was observed in Guangdong,China.Early symptom appeared as circular or irregularly shaped and dark brown spots along the leaf margins and tips.As the disease progressed,the spots...A severe leaf spot disease on patchouli,was observed in Guangdong,China.Early symptom appeared as circular or irregularly shaped and dark brown spots along the leaf margins and tips.As the disease progressed,the spots enlarged and with masses black,sessile conidiomata were observed in lesions.The results of morphology,molecular biology and pathogenicity test indicate that the causal agent of leaf disease of patchouli is Diaporthe arecae.展开更多
[Objective]The study was to evaluate the indoor toxicity and field efficacy of five Strobilurins fungicides including pyraclostrobin 250 g/L EC,azoxystrobin 250 g/L SC,kresoxim-methyl 50%WG,picoxystrobin 22.5%SC and t...[Objective]The study was to evaluate the indoor toxicity and field efficacy of five Strobilurins fungicides including pyraclostrobin 250 g/L EC,azoxystrobin 250 g/L SC,kresoxim-methyl 50%WG,picoxystrobin 22.5%SC and trifloxystrobin 50%WG against Phomopsis asparagi(Sacc.)Bubak,and to screen out effective fungicides.[Method]The toxicity was tested by mycelial growth rate method.The field trials were carried out by routine spraying method.[Result]The indoor toxicities successively were pyraclostrobin>kresoxim-methyl>picoxystrobin>trifloxystrobin>azoxystrobin.The EC50 values were 6.1029,52.5591,83.8257,129.8616 and 252.1214 mg/L,respectively,all significantly higher than that of benzimidazole fungicide carbendazim(753.3650 mg/L).The field efficacies successively were pyraclostrobin>kresoxim-methyl≥picoxystrobin≥azoxystrobin≥trifloxystrobin.The control effects were 81.20%,77.95%,74.80%,71.69%and 68.54%,respectively,all significantly higher than that of benzimidazole fungicide carbendazim(48.27%).[Conclusion]The five Strobilurins fungicides all have good toxicity and field efficacy against P.asparagi.展开更多
Eggplant phomopsis rot was summarized from the aspects of distribution and damage, pathogen identification and pathogenicity differentiation, and disease-resistant breeding. Furthermore, some existing problems and res...Eggplant phomopsis rot was summarized from the aspects of distribution and damage, pathogen identification and pathogenicity differentiation, and disease-resistant breeding. Furthermore, some existing problems and research directions in future was also discussed.展开更多
The export oriented agricultural and horticultural crops depends on the export of residue free produce and has created a great potential and demand for the incorporation of biopesticides in crop protection. PGPR with ...The export oriented agricultural and horticultural crops depends on the export of residue free produce and has created a great potential and demand for the incorporation of biopesticides in crop protection. PGPR with wide scope for commercialization includes Pseudomonas putida. Pseudomonas putida was effective in reducing die back caused by a fungus Botryodiplodia theobromae and phomopsis or arm death, caused by a fungus, Phomopsis viticola of grapevine in vitro and in vivo. Pseudomonas putida showed optimum Siderophore pseudobactin production at 72 h, and growth peak at 120 h. glycerol as a source of carbon and yeast as a source of nitrogen Pseudomonas putida was formulated using polysaccharide dextran starch, which led to the elongation of conservation and the effectiveness of the biofungicide. Pseudomonas putida was very effective as biocontrol agents to reduce the die back and phomopsis diseases on grapevine. The obtained results showed that both bacterial treatments significantly increased the growth parameters as well as fruits dry weights and yield of grapevine.展开更多
AIM: To study the chemical constituents of the solid culture of the endophyte Phomopsis sp. IFB-E060 in Vatica mangachapoi. METHOD: Isolation and purification were performed through silica gel column chromatography, g...AIM: To study the chemical constituents of the solid culture of the endophyte Phomopsis sp. IFB-E060 in Vatica mangachapoi. METHOD: Isolation and purification were performed through silica gel column chromatography, gel filtration over Sephadex LH-20, ODS column chromatography, and HPLC. Structures of the isolated compounds were elucidated by a combination of spectroscopic analyses(UV, CD, IR, MS, 1D, and 2D NMR). The cytotoxicity of the isolates was evaluated in vitro by the MTT method against the human hepatocarcinoma cell line SMMC-7721. RESULTS: Five compounds were isolated from the solid culture of the endophyte Phomopsis sp. IFB-E060 and their structures were identified as 18-methoxy cytochalasin J(1), cytochalasin H(2),(22E, 24S)-cerevisterol(3), ergosterol(4), and nicotinic acid(5). Compound 1 had an inhibition rate of 24.4% at 10 μg·mL-1 and 2 had an IC50 value of 15.0 μg·mL-1, while a positive control 5-fluorouracil had an inhibition rate of 28.7% at 10 μg·mL-1. CONCLUSION: 18-Methoxy cytochalasin J(1), produced by endophytic Phomopsis sp. IFB-E060, is a new cytochalasin with weak cytotoxicity to the human hepatocarcinoma cell line SMMC-7721.展开更多
Lithocarpinols A(1) and B(2), a pair of tenellone diastereoisomers with novel fused skeleton were isolated from the deep-sea derived fungus Phomopsis lithocarpus FS508. Their structures were elucidated by comprehensiv...Lithocarpinols A(1) and B(2), a pair of tenellone diastereoisomers with novel fused skeleton were isolated from the deep-sea derived fungus Phomopsis lithocarpus FS508. Their structures were elucidated by comprehensive spectroscopic analyses, X-ray diffraction and quantum molecular calculation. Their plausible biogenetic pathway featured an intriguing carbonyl-ene cyclization. Lithocarpinol A exhibited moderate inhibitory effect against HepG-2 and A549 tumor cell lines with IC_(50) values of 9.4 μmol/L and10.9 μmol/L,respectively.展开更多
Three new chromone derivatives, phomochromenones A-C （1-3）, and one known chaetocyclinone B （4） were obtained from the cultures ofPhomopsis sp. HNY29-2B isolated from the mangrove Acanthus ilicifolius Linn., which...Three new chromone derivatives, phomochromenones A-C （1-3）, and one known chaetocyclinone B （4） were obtained from the cultures ofPhomopsis sp. HNY29-2B isolated from the mangrove Acanthus ilicifolius Linn., which was collected from the South China Sea. Their structures were determined by the analysis of 1D NMR and 2D NMR as well as mass spectroscopic data. The absolute configurations of I and 2 were assigned by quantum chemi- cal calculations of the electronic circular dichroism （ECD） spectra. Compound 3 is the third example of alkaloids possessing the unique chromeno [3,2-c] pyridine nucleus. In the bioactivity assay, compound 4 showed cytotoxicity against human prostate cancer cell lines （PC-3 and DU145 cells） with the IC50 values of 8.13 and 3.59 μmol/L, respectively.展开更多
基金Supported by the National Natural Science Foundation of China(30770073,30500066)~~
文摘After insufflating extended endophytes phomopsis B3 ferment diluent on seedling bed of rice, the effect of microbial inoculum on rice growth was studied. The investigation result of seedlings before transplanting seedling showed that leaf age,the number of green leaves,the number of tiller of individual plant and the weight of per 100 fresh plants in microbial inoculum treatment group increased 0.11,0.12 ,0. 03 and 2.6 g respectively compared with those of control group. 20 days after transplanting, increased tiller number in microbial inoculum treatment group was 55 500 hm^2 more than that in control group. 30 days after transplanting, tiller number in microbial inoculum treatment group was 42 000 hm^2 more than that in control group. In harvest season, the yield ,weight of I 0130 grain and seed-setting rate increased 300 kg/hm2, 0. 7 g and 0. 23% respectively compared with these in control group. The result of preliminary study demonstrated that microbial inoculum confected by endophytes phomopsis B3 could increase the number of strong seedling and yield.
文摘从云南美登木内生真菌Phomopsis species Lz42的琼脂平板发酵物中分离得到2个倍半萜和5个麦角甾醇类化合物(1～7),其中化合物1和2为新化合物.应用波谱技术确定其结构为4-Deacetyl-10-oxo-di-hydrobotrydial(1)和麦角甾-6,22-二烯-5α,8α-环二氧-3-甲酸酯(2).
文摘Fungal pathogen of asparagus stem blight was isolated. No significant genetic difference was detected among the three strains with 492 bp long ITS1-5.8S-ITS2 sequence. It was then identified through colony growth, conidia morphology, and molecular characterization. The physiological response to oxidation and osmosis stress, and virulence to Asparagus officinalis L. were analyzed. The results showed that the pathogen causing asparagus stem blight for A. officinalis L. in Jiangxi Province is Phomopsis asparagri (Sacc.) Bubák. Under pure culture conditions, the conidia were oval-shaped (α-type), with colorless single spore and single nucleus, containing 0-2 oil balls. Its vegetative growth rate was higher when cultured on 0.2 × potato dextrose agar (0.2 × PDA) medium than that on oatmeal agar (OA) medium. However, the pycnidia appeared earlier on OA medium than on 0.2 earlier PDA medium. The vegetative growth rate was depressed under oxidation (H2O2) or osmosis (NaCl) stress conditions, and totally inhibited under 7 mmol/L H2O2 or 2.4 mol/L NaCl. All the strains caused typical pathogenic symptoms to Asparagus officinalis L. at 7 days-post-inoculation (dpi) with conidia.
基金Supported by Science and Technology Project of Guangdong Province in China(2017A020213003)the Opening Project Fund of Key Laboratory of Guangdong Institute of Traditional Chinese Medicine(GDTCMZJ201901)。
文摘A severe leaf spot disease on patchouli,was observed in Guangdong,China.Early symptom appeared as circular or irregularly shaped and dark brown spots along the leaf margins and tips.As the disease progressed,the spots enlarged and with masses black,sessile conidiomata were observed in lesions.The results of morphology,molecular biology and pathogenicity test indicate that the causal agent of leaf disease of patchouli is Diaporthe arecae.
基金Supported by Agricultural Science and Technology Innovation Project of Shandong Academy of Agricultural Sciences"Key Technology for Green Prevention and Control of Diseases and Insect Pests of Major Crops"(CXGC2018E19).
文摘[Objective]The study was to evaluate the indoor toxicity and field efficacy of five Strobilurins fungicides including pyraclostrobin 250 g/L EC,azoxystrobin 250 g/L SC,kresoxim-methyl 50%WG,picoxystrobin 22.5%SC and trifloxystrobin 50%WG against Phomopsis asparagi(Sacc.)Bubak,and to screen out effective fungicides.[Method]The toxicity was tested by mycelial growth rate method.The field trials were carried out by routine spraying method.[Result]The indoor toxicities successively were pyraclostrobin>kresoxim-methyl>picoxystrobin>trifloxystrobin>azoxystrobin.The EC50 values were 6.1029,52.5591,83.8257,129.8616 and 252.1214 mg/L,respectively,all significantly higher than that of benzimidazole fungicide carbendazim(753.3650 mg/L).The field efficacies successively were pyraclostrobin>kresoxim-methyl≥picoxystrobin≥azoxystrobin≥trifloxystrobin.The control effects were 81.20%,77.95%,74.80%,71.69%and 68.54%,respectively,all significantly higher than that of benzimidazole fungicide carbendazim(48.27%).[Conclusion]The five Strobilurins fungicides all have good toxicity and field efficacy against P.asparagi.
基金Supported by Project of Jilin Department of Education(2013076)
文摘Eggplant phomopsis rot was summarized from the aspects of distribution and damage, pathogen identification and pathogenicity differentiation, and disease-resistant breeding. Furthermore, some existing problems and research directions in future was also discussed.
文摘The export oriented agricultural and horticultural crops depends on the export of residue free produce and has created a great potential and demand for the incorporation of biopesticides in crop protection. PGPR with wide scope for commercialization includes Pseudomonas putida. Pseudomonas putida was effective in reducing die back caused by a fungus Botryodiplodia theobromae and phomopsis or arm death, caused by a fungus, Phomopsis viticola of grapevine in vitro and in vivo. Pseudomonas putida showed optimum Siderophore pseudobactin production at 72 h, and growth peak at 120 h. glycerol as a source of carbon and yeast as a source of nitrogen Pseudomonas putida was formulated using polysaccharide dextran starch, which led to the elongation of conservation and the effectiveness of the biofungicide. Pseudomonas putida was very effective as biocontrol agents to reduce the die back and phomopsis diseases on grapevine. The obtained results showed that both bacterial treatments significantly increased the growth parameters as well as fruits dry weights and yield of grapevine.
基金supported by the Natural Science Foundation of China(Nos.81121062,30701045,21132004&21072092)the Natural Science Foundation of Jiangsu Province(No.BK2009010)the Social Development Project of Yangzhou(No.YZ2011097)
文摘AIM: To study the chemical constituents of the solid culture of the endophyte Phomopsis sp. IFB-E060 in Vatica mangachapoi. METHOD: Isolation and purification were performed through silica gel column chromatography, gel filtration over Sephadex LH-20, ODS column chromatography, and HPLC. Structures of the isolated compounds were elucidated by a combination of spectroscopic analyses(UV, CD, IR, MS, 1D, and 2D NMR). The cytotoxicity of the isolates was evaluated in vitro by the MTT method against the human hepatocarcinoma cell line SMMC-7721. RESULTS: Five compounds were isolated from the solid culture of the endophyte Phomopsis sp. IFB-E060 and their structures were identified as 18-methoxy cytochalasin J(1), cytochalasin H(2),(22E, 24S)-cerevisterol(3), ergosterol(4), and nicotinic acid(5). Compound 1 had an inhibition rate of 24.4% at 10 μg·mL-1 and 2 had an IC50 value of 15.0 μg·mL-1, while a positive control 5-fluorouracil had an inhibition rate of 28.7% at 10 μg·mL-1. CONCLUSION: 18-Methoxy cytochalasin J(1), produced by endophytic Phomopsis sp. IFB-E060, is a new cytochalasin with weak cytotoxicity to the human hepatocarcinoma cell line SMMC-7721.
基金financially supported by the Science and Technology Program of Guangzhou, China(No. 201607020018)the Team Project of the Natural Science Foundation of Guangdong Province (No. 2016A030312014)+2 种基金the National Natural Science Foundation of China (No. 31272087)the Guangdong Provincial Project for Science and Technology (Nos. 2015A030302061, 2016A020222022)the Guangdong Provincial Innovative Development of Marine Economy Regional Demonstration Projects (No. GD2012-D01-002)
文摘Lithocarpinols A(1) and B(2), a pair of tenellone diastereoisomers with novel fused skeleton were isolated from the deep-sea derived fungus Phomopsis lithocarpus FS508. Their structures were elucidated by comprehensive spectroscopic analyses, X-ray diffraction and quantum molecular calculation. Their plausible biogenetic pathway featured an intriguing carbonyl-ene cyclization. Lithocarpinol A exhibited moderate inhibitory effect against HepG-2 and A549 tumor cell lines with IC_(50) values of 9.4 μmol/L and10.9 μmol/L,respectively.
基金Supporting information for this article is available on the WWW under http：//dx.doi.org/10.1002/cjoc.201700375 or from the author.Acknowledgement We thank the National Natural Science Foundation of China （Nos. 21472251, 41276146）, the Key Project of Natural Science Foundation of Guangdong Province （No. 2016A030311026）, the Science ＆ Technology Plan Project of Guangdong Province of China （Nos. 2013B021100011, 2016A040403091）, the Special Financial Fund of Innovative Development of Marine Economic Demonstration Project （No. GD2012-D01- 001）, the China＇s Marine Commonweal Research Project （No. 201305017）, the Guangzhou Project of Science ＆ Technology Planning （No. 201300000062）, the Fundamental Research Funds for the Central Universities （No. 141gjc16） and the Supercomputing Center of Chinese Academy of Sciences （SCCAS） for generous support.
文摘Three new chromone derivatives, phomochromenones A-C （1-3）, and one known chaetocyclinone B （4） were obtained from the cultures ofPhomopsis sp. HNY29-2B isolated from the mangrove Acanthus ilicifolius Linn., which was collected from the South China Sea. Their structures were determined by the analysis of 1D NMR and 2D NMR as well as mass spectroscopic data. The absolute configurations of I and 2 were assigned by quantum chemi- cal calculations of the electronic circular dichroism （ECD） spectra. Compound 3 is the third example of alkaloids possessing the unique chromeno [3,2-c] pyridine nucleus. In the bioactivity assay, compound 4 showed cytotoxicity against human prostate cancer cell lines （PC-3 and DU145 cells） with the IC50 values of 8.13 and 3.59 μmol/L, respectively.
