从云南美登木内生真菌Phomopsis species Lz42的琼脂平板发酵物中分离得到2个倍半萜和5个麦角甾醇类化合物(1～7),其中化合物1和2为新化合物.应用波谱技术确定其结构为4-Deacetyl-10-oxo-di-hydrobotrydial(1)和麦角甾-6,22-二烯-5α,8...从云南美登木内生真菌Phomopsis species Lz42的琼脂平板发酵物中分离得到2个倍半萜和5个麦角甾醇类化合物(1～7),其中化合物1和2为新化合物.应用波谱技术确定其结构为4-Deacetyl-10-oxo-di-hydrobotrydial(1)和麦角甾-6,22-二烯-5α,8α-环二氧-3-甲酸酯(2).展开更多
从表现桑椹菌核病病症的桑树病果中分离到一株优势真菌菌株,菌株在PDA平板上培养的菌落外观特征与在染病桑椹(病果)小核果间菌丝发育形成的菌核病菌类似,均出现黑色菌丝团,但新分离株的黑色菌丝团不形成菌核。通过对菌株r DNA ITS 583 b...从表现桑椹菌核病病症的桑树病果中分离到一株优势真菌菌株,菌株在PDA平板上培养的菌落外观特征与在染病桑椹(病果)小核果间菌丝发育形成的菌核病菌类似,均出现黑色菌丝团,但新分离株的黑色菌丝团不形成菌核。通过对菌株r DNA ITS 583 bp片段序列(Gen Bank登录号:KT824639)的PCR检测,并结合菌株的形态学特征和系统发育分析,初步鉴定该菌株属于拟茎点霉(Phomopsis sp.SC1104)。该菌株的致病性试验表明,桑树花期受感染后,在桑椹即将成熟时表现出病理特征,病果整个或者部分呈白色,在病果的小果间出现不规则的黑色菌丝团,并能从病果中重复分离和检测到相同的病菌。试验分析显示Phomopsis sp.SC1104菌株可感染桑椹致病,推测该菌株或作为潜伏性病原菌在桑椹菌核病的大面积暴发中扮演了重要角色。展开更多
A severe leaf spot disease on patchouli,was observed in Guangdong,China.Early symptom appeared as circular or irregularly shaped and dark brown spots along the leaf margins and tips.As the disease progressed,the spots...A severe leaf spot disease on patchouli,was observed in Guangdong,China.Early symptom appeared as circular or irregularly shaped and dark brown spots along the leaf margins and tips.As the disease progressed,the spots enlarged and with masses black,sessile conidiomata were observed in lesions.The results of morphology,molecular biology and pathogenicity test indicate that the causal agent of leaf disease of patchouli is Diaporthe arecae.展开更多
Eggplant phomopsis rot was summarized from the aspects of distribution and damage, pathogen identification and pathogenicity differentiation, and disease-resistant breeding. Furthermore, some existing problems and res...Eggplant phomopsis rot was summarized from the aspects of distribution and damage, pathogen identification and pathogenicity differentiation, and disease-resistant breeding. Furthermore, some existing problems and research directions in future was also discussed.展开更多
[Objective]The study was to evaluate the indoor toxicity and field efficacy of five Strobilurins fungicides including pyraclostrobin 250 g/L EC,azoxystrobin 250 g/L SC,kresoxim-methyl 50%WG,picoxystrobin 22.5%SC and t...[Objective]The study was to evaluate the indoor toxicity and field efficacy of five Strobilurins fungicides including pyraclostrobin 250 g/L EC,azoxystrobin 250 g/L SC,kresoxim-methyl 50%WG,picoxystrobin 22.5%SC and trifloxystrobin 50%WG against Phomopsis asparagi(Sacc.)Bubak,and to screen out effective fungicides.[Method]The toxicity was tested by mycelial growth rate method.The field trials were carried out by routine spraying method.[Result]The indoor toxicities successively were pyraclostrobin>kresoxim-methyl>picoxystrobin>trifloxystrobin>azoxystrobin.The EC50 values were 6.1029,52.5591,83.8257,129.8616 and 252.1214 mg/L,respectively,all significantly higher than that of benzimidazole fungicide carbendazim(753.3650 mg/L).The field efficacies successively were pyraclostrobin>kresoxim-methyl≥picoxystrobin≥azoxystrobin≥trifloxystrobin.The control effects were 81.20%,77.95%,74.80%,71.69%and 68.54%,respectively,all significantly higher than that of benzimidazole fungicide carbendazim(48.27%).[Conclusion]The five Strobilurins fungicides all have good toxicity and field efficacy against P.asparagi.展开更多
The export oriented agricultural and horticultural crops depends on the export of residue free produce and has created a great potential and demand for the incorporation of biopesticides in crop protection. PGPR with ...The export oriented agricultural and horticultural crops depends on the export of residue free produce and has created a great potential and demand for the incorporation of biopesticides in crop protection. PGPR with wide scope for commercialization includes Pseudomonas putida. Pseudomonas putida was effective in reducing die back caused by a fungus Botryodiplodia theobromae and phomopsis or arm death, caused by a fungus, Phomopsis viticola of grapevine in vitro and in vivo. Pseudomonas putida showed optimum Siderophore pseudobactin production at 72 h, and growth peak at 120 h. glycerol as a source of carbon and yeast as a source of nitrogen Pseudomonas putida was formulated using polysaccharide dextran starch, which led to the elongation of conservation and the effectiveness of the biofungicide. Pseudomonas putida was very effective as biocontrol agents to reduce the die back and phomopsis diseases on grapevine. The obtained results showed that both bacterial treatments significantly increased the growth parameters as well as fruits dry weights and yield of grapevine.展开更多
文摘从云南美登木内生真菌Phomopsis species Lz42的琼脂平板发酵物中分离得到2个倍半萜和5个麦角甾醇类化合物(1～7),其中化合物1和2为新化合物.应用波谱技术确定其结构为4-Deacetyl-10-oxo-di-hydrobotrydial(1)和麦角甾-6,22-二烯-5α,8α-环二氧-3-甲酸酯(2).
文摘从表现桑椹菌核病病症的桑树病果中分离到一株优势真菌菌株,菌株在PDA平板上培养的菌落外观特征与在染病桑椹(病果)小核果间菌丝发育形成的菌核病菌类似,均出现黑色菌丝团,但新分离株的黑色菌丝团不形成菌核。通过对菌株r DNA ITS 583 bp片段序列(Gen Bank登录号:KT824639)的PCR检测,并结合菌株的形态学特征和系统发育分析,初步鉴定该菌株属于拟茎点霉(Phomopsis sp.SC1104)。该菌株的致病性试验表明,桑树花期受感染后,在桑椹即将成熟时表现出病理特征,病果整个或者部分呈白色,在病果的小果间出现不规则的黑色菌丝团,并能从病果中重复分离和检测到相同的病菌。试验分析显示Phomopsis sp.SC1104菌株可感染桑椹致病,推测该菌株或作为潜伏性病原菌在桑椹菌核病的大面积暴发中扮演了重要角色。
基金Supported by Science and Technology Project of Guangdong Province in China(2017A020213003)the Opening Project Fund of Key Laboratory of Guangdong Institute of Traditional Chinese Medicine(GDTCMZJ201901)。
文摘A severe leaf spot disease on patchouli,was observed in Guangdong,China.Early symptom appeared as circular or irregularly shaped and dark brown spots along the leaf margins and tips.As the disease progressed,the spots enlarged and with masses black,sessile conidiomata were observed in lesions.The results of morphology,molecular biology and pathogenicity test indicate that the causal agent of leaf disease of patchouli is Diaporthe arecae.
基金Supported by Project of Jilin Department of Education(2013076)
文摘Eggplant phomopsis rot was summarized from the aspects of distribution and damage, pathogen identification and pathogenicity differentiation, and disease-resistant breeding. Furthermore, some existing problems and research directions in future was also discussed.
基金Supported by Agricultural Science and Technology Innovation Project of Shandong Academy of Agricultural Sciences"Key Technology for Green Prevention and Control of Diseases and Insect Pests of Major Crops"(CXGC2018E19).
文摘[Objective]The study was to evaluate the indoor toxicity and field efficacy of five Strobilurins fungicides including pyraclostrobin 250 g/L EC,azoxystrobin 250 g/L SC,kresoxim-methyl 50%WG,picoxystrobin 22.5%SC and trifloxystrobin 50%WG against Phomopsis asparagi(Sacc.)Bubak,and to screen out effective fungicides.[Method]The toxicity was tested by mycelial growth rate method.The field trials were carried out by routine spraying method.[Result]The indoor toxicities successively were pyraclostrobin>kresoxim-methyl>picoxystrobin>trifloxystrobin>azoxystrobin.The EC50 values were 6.1029,52.5591,83.8257,129.8616 and 252.1214 mg/L,respectively,all significantly higher than that of benzimidazole fungicide carbendazim(753.3650 mg/L).The field efficacies successively were pyraclostrobin>kresoxim-methyl≥picoxystrobin≥azoxystrobin≥trifloxystrobin.The control effects were 81.20%,77.95%,74.80%,71.69%and 68.54%,respectively,all significantly higher than that of benzimidazole fungicide carbendazim(48.27%).[Conclusion]The five Strobilurins fungicides all have good toxicity and field efficacy against P.asparagi.
文摘The export oriented agricultural and horticultural crops depends on the export of residue free produce and has created a great potential and demand for the incorporation of biopesticides in crop protection. PGPR with wide scope for commercialization includes Pseudomonas putida. Pseudomonas putida was effective in reducing die back caused by a fungus Botryodiplodia theobromae and phomopsis or arm death, caused by a fungus, Phomopsis viticola of grapevine in vitro and in vivo. Pseudomonas putida showed optimum Siderophore pseudobactin production at 72 h, and growth peak at 120 h. glycerol as a source of carbon and yeast as a source of nitrogen Pseudomonas putida was formulated using polysaccharide dextran starch, which led to the elongation of conservation and the effectiveness of the biofungicide. Pseudomonas putida was very effective as biocontrol agents to reduce the die back and phomopsis diseases on grapevine. The obtained results showed that both bacterial treatments significantly increased the growth parameters as well as fruits dry weights and yield of grapevine.
