A vacuolar ATPase (V-ATPase.) B subunit gene has been cloned and characterized front a phosphorus starvation induced rice root subtractive cDNA library by suppression subtractive hybridization (SSH) method and RT-PCR ...A vacuolar ATPase (V-ATPase.) B subunit gene has been cloned and characterized front a phosphorus starvation induced rice root subtractive cDNA library by suppression subtractive hybridization (SSH) method and RT-PCR amplification. This gene encodes a polypeptide of 487 amino acid residues, containing a conservative ATP binding site and with a molecular weight of 54.06 kD and an isoelectric point of 4.99, southern analysis of the. genomic DNA indicates that V-ATPase B subunit is encoded by a single gene in rice genome. The amino acid homologies of V-ATPase B subunits among different organisms range from 76% to 97% and reveals that the evolution of V-ATPase B subunit is accompanied with the biological evolution. Expression pattern analysis indicated that the maximal expression of V-ATPase B subunit gene occurred at an early stage (6 - 12 h) after phosphorus starvation in roots, and lately stage (24 - 48 It) in leaves. Under phosphorus deficiency, the up-regulated expression of V-ATPase gene was presumed to strengthen the proton transport and provide the required energy to maintain an electrochemical gradient across the tonoplast to facilitate Phosphorus transport.展开更多
Corn and wheat plants were grown in a nutrient culture solution at four levels of phosphorus (0, 0.12,0.60 and 3.0 mmol L-1) and two levels of cadmium (0 and 4.0 pmol L--1) in greenhouse for a 18-day period.The concen...Corn and wheat plants were grown in a nutrient culture solution at four levels of phosphorus (0, 0.12,0.60 and 3.0 mmol L-1) and two levels of cadmium (0 and 4.0 pmol L--1) in greenhouse for a 18-day period.The concentrations of phosphorus and cadmium in cell wall, cytoplasm and vacuoles of roots and leaveswere examined by cell fractionation techniques. With increasing phosphorus in medium, the contents of Pin cell wall, cytoplasm and vacuoles of corn and wheat roots and leaves increased. The highest content of Pwas observed in cell wall, next in vacuoles, and the lowest in cytoplasm. The wheat subcellular fractions inboth roots and leaves had higher concentrations of phosphorus than those of corn. Increasing phosphorus inmedium significantly inhibited the intracellular Cd accumulation in both species. However, at P concentrationup to 3.0 mmol L--1, the Cd content in cell wall was increased. Increasing phosphorus resulted in reductionof the subcellular Cd content in corn and wheat leaves. Compared with corn, the wheat roots had a higherCd content in the cell wall and vacuoles and a lower in cytoplasm, while in leaf subcellular fractions thewheat cell had a higher Cd content in its vacuoles and a lower one in its cytoplasm. The results indicate thatphosphorus may be involved in sequestration of Cd ionic activity in both cell wall and vacuoles by forminginsoluble Cd phosphate.展开更多
骨髓增生异常肿瘤(myelodysplastic neoplasms,MDS)患者的预后与其细胞遗传学和分子遗传学特征密切相关,TP53基因作为MDS最常见的突变基因之一,其异常是MDS独立的不良预后因素以及向急性髓系白血病(acute myeloid leuke⁃mia,AML)转化的...骨髓增生异常肿瘤(myelodysplastic neoplasms,MDS)患者的预后与其细胞遗传学和分子遗传学特征密切相关,TP53基因作为MDS最常见的突变基因之一,其异常是MDS独立的不良预后因素以及向急性髓系白血病(acute myeloid leuke⁃mia,AML)转化的危险因素之一。新近更新的第5版世界卫生组织血液淋巴肿瘤分类标准(the 5th edition of the who classifica⁃tion of haematolymphoid tumours,WHO 2022)以及髓系肿瘤和急性白血病的国际共识分类(international consensus classification of myeloid neoplasms and acute leukemia,ICC)将伴有TP53双等位基因失活(biallelic TP53 inactivation,biTP53)的MDS作为独立的亚型列出,该亚型患者预后极差,生存期极短。目前针对伴TP53基因异常的MDS患者的治疗尚未能有效改善该类患者预后,新型靶向药物、免疫治疗等均在探索中,本文就国内外对伴TP53基因异常的MDS的临床研究进展进行综述。展开更多
[ Objective] The study aimed to reveal the output characteristics of non-point nitrogen and phosphorus from a typical small watershed in Yimeng mountainous area during a rainstorm. [Method] The dynamic changes of poll...[ Objective] The study aimed to reveal the output characteristics of non-point nitrogen and phosphorus from a typical small watershed in Yimeng mountainous area during a rainstorm. [Method] The dynamic changes of pollutant concentration, precipitation and flow during the rainstorm on August 12, 2010 were monitored at the outlet of Menglianggu watershed. [ Result] During the rainstorm, the generation of runoff was sudden and ephemeral, and the peak of the runoff lagged behind that of rainfall intensity; the concentration of AN and TN increased firstly and then tended to be stable, while NN concentration had no significance change at the beginning of the rainfall, then improved gradually and tended to be stable fi- nally; DOP concentration had no obvious change during the rainstorm, but the concentration of DIP, DP, PP and TP rose firstly and then tended to be stable, and the peak values appeared before the peak of the flow. In addition, the output concentration of TN and TP was far higher than the standard concentration of water eutrophication. [ Conclusion] The study can provide scientific references for the reasonable control of non-point source pollution pollution in Yimeng mountainous area.展开更多
文摘A vacuolar ATPase (V-ATPase.) B subunit gene has been cloned and characterized front a phosphorus starvation induced rice root subtractive cDNA library by suppression subtractive hybridization (SSH) method and RT-PCR amplification. This gene encodes a polypeptide of 487 amino acid residues, containing a conservative ATP binding site and with a molecular weight of 54.06 kD and an isoelectric point of 4.99, southern analysis of the. genomic DNA indicates that V-ATPase B subunit is encoded by a single gene in rice genome. The amino acid homologies of V-ATPase B subunits among different organisms range from 76% to 97% and reveals that the evolution of V-ATPase B subunit is accompanied with the biological evolution. Expression pattern analysis indicated that the maximal expression of V-ATPase B subunit gene occurred at an early stage (6 - 12 h) after phosphorus starvation in roots, and lately stage (24 - 48 It) in leaves. Under phosphorus deficiency, the up-regulated expression of V-ATPase gene was presumed to strengthen the proton transport and provide the required energy to maintain an electrochemical gradient across the tonoplast to facilitate Phosphorus transport.
文摘Corn and wheat plants were grown in a nutrient culture solution at four levels of phosphorus (0, 0.12,0.60 and 3.0 mmol L-1) and two levels of cadmium (0 and 4.0 pmol L--1) in greenhouse for a 18-day period.The concentrations of phosphorus and cadmium in cell wall, cytoplasm and vacuoles of roots and leaveswere examined by cell fractionation techniques. With increasing phosphorus in medium, the contents of Pin cell wall, cytoplasm and vacuoles of corn and wheat roots and leaves increased. The highest content of Pwas observed in cell wall, next in vacuoles, and the lowest in cytoplasm. The wheat subcellular fractions inboth roots and leaves had higher concentrations of phosphorus than those of corn. Increasing phosphorus inmedium significantly inhibited the intracellular Cd accumulation in both species. However, at P concentrationup to 3.0 mmol L--1, the Cd content in cell wall was increased. Increasing phosphorus resulted in reductionof the subcellular Cd content in corn and wheat leaves. Compared with corn, the wheat roots had a higherCd content in the cell wall and vacuoles and a lower in cytoplasm, while in leaf subcellular fractions thewheat cell had a higher Cd content in its vacuoles and a lower one in its cytoplasm. The results indicate thatphosphorus may be involved in sequestration of Cd ionic activity in both cell wall and vacuoles by forminginsoluble Cd phosphate.
文摘骨髓增生异常肿瘤(myelodysplastic neoplasms,MDS)患者的预后与其细胞遗传学和分子遗传学特征密切相关,TP53基因作为MDS最常见的突变基因之一,其异常是MDS独立的不良预后因素以及向急性髓系白血病(acute myeloid leuke⁃mia,AML)转化的危险因素之一。新近更新的第5版世界卫生组织血液淋巴肿瘤分类标准(the 5th edition of the who classifica⁃tion of haematolymphoid tumours,WHO 2022)以及髓系肿瘤和急性白血病的国际共识分类(international consensus classification of myeloid neoplasms and acute leukemia,ICC)将伴有TP53双等位基因失活(biallelic TP53 inactivation,biTP53)的MDS作为独立的亚型列出,该亚型患者预后极差,生存期极短。目前针对伴TP53基因异常的MDS患者的治疗尚未能有效改善该类患者预后,新型靶向药物、免疫治疗等均在探索中,本文就国内外对伴TP53基因异常的MDS的临床研究进展进行综述。
基金Supported by Science and Technology Innovation Project of Linyi City(201011019)Science and Technology Key Project of Shandong Province(2009GG10006015)Science and Technology Project of Huaihe River Commission,the Ministry of Water Resources (SBJ2010003)
文摘[ Objective] The study aimed to reveal the output characteristics of non-point nitrogen and phosphorus from a typical small watershed in Yimeng mountainous area during a rainstorm. [Method] The dynamic changes of pollutant concentration, precipitation and flow during the rainstorm on August 12, 2010 were monitored at the outlet of Menglianggu watershed. [ Result] During the rainstorm, the generation of runoff was sudden and ephemeral, and the peak of the runoff lagged behind that of rainfall intensity; the concentration of AN and TN increased firstly and then tended to be stable, while NN concentration had no significance change at the beginning of the rainfall, then improved gradually and tended to be stable fi- nally; DOP concentration had no obvious change during the rainstorm, but the concentration of DIP, DP, PP and TP rose firstly and then tended to be stable, and the peak values appeared before the peak of the flow. In addition, the output concentration of TN and TP was far higher than the standard concentration of water eutrophication. [ Conclusion] The study can provide scientific references for the reasonable control of non-point source pollution pollution in Yimeng mountainous area.
