未知人Na(+)-K(+)-Exchanging ATPase α1亚基基因第一内含子的获得及鉴定

目的研究人Ｎａ（＋）－Ｋ（＋）－ＥｘｃｈａｎｇｉｎｇＡＴＰａｓｅα１亚单位基因胞外区约８０～１３０位氨基酸编码序列的未知基因组结构。方法采用聚合酶链反应（ＰＣＲ）方法对人基因组ＤＮＡ及ｃＤＮＡ文库进行扩增，限制性酶切分析扩增产物，并进行荧光测序，对测序结果进行同源性分析及剪接位点的搜索并对得到的核苷酸序列进行分析。结果人基因组ＤＮＡ和ｃＤＮＡ经扩增后分别得到８３３和１９５ｂｐ两种不同大小的片段Ｆｇ，Ｆｃ。分析序列发现Ｆｇ与Ｆｃ相比在１３８～７７５ｂｐ处含有一６３８ｂｐ的插入片段，此片段与ＧｅｎＢａｎｋ中的任何已知序列均无明显同源性。结论本研究发现了一段全新未知的人Ｎａ（＋）－Ｋ（＋）－ＥｘｃｈａｎｇｉｎｇＡＴＰａｓｅα１亚单位基因的内含子全序列，并得到了第一外显子同第二外显子之间的相对位置和序列，其在Ｇｅｎ－Ｂａｎｋ的基因检索号为Ｌ７６９３８。序列分析表明该内含子可能具有潜在的调控基因表达的功能，并含有一个可能的编码序列。

Preparation of SiO_2 Nanoparticles by Ion Exchanging and Study on Their Dispersion Stability

By adopting sodium silicate as a major material,SiO_2 nanoparticles (size in 8-15nm) water-dispersiod was prepared by ion exchanging.The effects of sodium silicate concentration,surface-modifying time,temperature and technological conditions on their diameter,size distribution and dispersion stability were also studied.The result show that,the hydrophilic lipophlic and hydrophilic-lipophilic SiO_(2) nanoparticles water-dispersoid can be prepared through different kinds of surface-modifiers and the optimum reaction conditions have been determined as follows:sodium silicate solution concentration:8w%;silicone dosage:3% of the total mass of nano SiO_(2) water-dispersoid;adding way and time of surface-modifier:continual dropping for 2h;surface-modifying temperature:60-70℃.

Molecular Exchanging Energy of Anionic/Cationic Surfactants System on the Surface of Solution

In order to study synergism of the mixed surfactants system with molecular exchanging energy (E), the Lennard-Jones formula has been firstly introduced to evaluate the E of the mixed system, CH3(CH2)nOSO 3 /CH3(CH2)nN+(CH3)3 directly from their molecular structure. The comparison of the calculated and the observed results showed that this method is practical.

Development of advanced anion exchange membrane from the view of the performance of water electrolysis cell

Green hydrogen produced by water electrolysis combined with renewable energy is a promising alternative to fossil fuels due to its high energy density with zero-carbon emissions.Among water electrolysis technologies,the anion exchange membrane(AEM) water electrolysis has gained intensive attention and is considered as the next-generation emerging technology due to its potential advantages,such as the use of low-cost non-noble metal catalysts,the relatively mature stack assembly process,etc.However,the AEM water electrolyzer is still in the early development stage of the kW-level stack,which is mainly attributed to severe performance decay caused by the core component,i.e.,AEM.Here,the review comprehensively presents the recent progress of advanced AEM from the view of the performance of water electrolysis cells.Herein,fundamental principles and critical components of AEM water electrolyzers are introduced,and work conditions of AEM water electrolyzers and AEM performance improvement strategies are discussed.The challenges and perspectives are also analyzed.

Proton‑Prompted Ligand Exchange to Achieve High‑Efficiency CsPbI_(3) Quantum Dot Light‑Emitting Diodes

CsPbI_(3)perovskite quantum dots(QDs)are ideal materials for the next generation of red light-emitting diodes.However,the low phase stability of CsPbI_(3)QDs and long-chain insulating capping ligands hinder the improvement of device performance.Traditional in-situ ligand replacement and ligand exchange after synthesis were often difficult to control.Here,we proposed a new ligand exchange strategy using a proton-prompted insitu exchange of short 5-aminopentanoic acid ligands with long-chain oleic acid and oleylamine ligands to obtain stable small-size CsPbI_(3)QDs.This exchange strategy maintained the size and morphology of CsPbI_(3)QDs and improved the optical properties and the conductivity of CsPbI_(3)QDs films.As a result,high-efficiency red QD-based light-emitting diodes with an emission wavelength of 645 nm demonstrated a record maximum external quantum efficiency of 24.45%and an operational half-life of 10.79 h.

Solar wind ion charge state distributions and compound cross sections for solar wind charge exchange X-ray emission

Solar Wind Charge eXchange X-ray(SWCX) emission in the heliosphere and Ea rth’s exosphere is a hard to avoid signal in soft Xray obse rvations of astrophysical targets.On the other hand,the X-ray imaging possibilities offered by the SWCX process has led to an increasing number of future dedicated space missions for investigating the solar wind-terrestrial inte ractions and magnetospheric interfaces.In both cases,accurate modelling of the SWCX emission is key to correctly interpret its signal,and remove it from obse rvations,when needed.In this paper,we compile solar wind abundance measurements from ACE for different solar wind types,and atomic data from literature,including charge exchange cross-sections and emission probabilities,used fo r calculating the compound cross-section a for the SWCX X-ray emission.We calculate a values for charge-exchange with H and He,relevant to soft X-ray energy bands(0.1-2.0 keV)for various solar wind types and solar cycle conditions.

Two methods for separating the magnetospheric solar wind charge exchange soft X-ray emission from the diffuse X-ray background

Solar wind charge exchange(SWCX)is the process of solar wind high-valence ions exchanging charges with neutral components and generating soft X-rays.Recently,detecting the SWCX emission from the magnetosphere is proposed as a new technique to study the magnetosphere using panoramic soft X-ray imaging.To better prepare for the data analysis of upcoming magnetospheric soft X-ray imaging missions,this paper compares the magnetospheric SWCX emission obtained by two methods in an XMM-Newton observation,during which the solar wind changed dramatically.The two methods differ in the data used to fit the diffuse X-ray background(DXB)parameters in spectral analysis.The method adding data from the ROSAT All-Sky Survey(RASS)is called the RASS method.The method using the quiet observation data is called the Quiet method,where quiet observations usually refer to observations made by the same satellite with the same target but under weaker solar wind conditions.Results show that the spectral compositions of magnetospheric SWCX emission obtained by the two methods are very similar,and the changes in intensity over time are highly consistent,although the intensity obtained by the RASS method is about 2.68±0.56 keV cm^(-2)s^(-1)sr^(-1)higher than that obtained by the Quiet method.Since the DXB intensity obtained by the RASS method is about 2.84±0.74 keV cm^(-2)s^(-1)sr^(-1)lower than that obtained by the Quiet method,and the linear correlation coefficient between the difference of SWCX and DXB obtained by the two methods in diffe rent energy band is close to-1,the diffe rences in magnetospheric SWCX can be fully attributed to the diffe rences in the fitted DXB.The difference between the two methods is most significant when the energy is less than 0.7 keV,which is also the main energy band of SWCX emission.In addition,the difference between the two methods is not related to the SWCX intensity and,to some extent,to solar wind conditions,because SWCX intensity typically va ries with the solar wind.In summary,both methods are robust and reliable,and should be considered based on the best available options.

Changes in soluble interleukin-2 receptor level in serum and Na^+ -K^+ -exchanging ATPase activity in semen of infertile men caused by antisperm antibody

Aim: To explore the possible mechanisms of male infertility caused by antisperm antibody (AsAb). Methods: Thesoluble interleukin-2 receptor (sIL-2R) level in serum was analyzed by ELISA and Na^+ -K^+ -exchanging ATPase activi-ty in semen by phosphorus (Pi) assay. Results: The slL-2R level in serum was significantly higher and the Na^+ -K^+ -exchanging ATPase activity in semen significantly lower in AsAb positive infertile men when compared with thecontrols. Conclusion: The AsAb titer varies with the slL-2R level in serum. A decrease in Na^+ -K^+ -exchangingATPase activity in semen may play a role in male infertility caused by AsAb.

Effects of drip and flood irrigation on carbon dioxide exchange and crop growth in the maize ecosystem in the Hetao Irrigation District,China

Drip irrigation and flood irrigation are major irrigation methods for maize crops in the Hetao Irrigation District,Inner Mongolia Autonomous Region,China.This research delves into the effects of these irrigation methods on carbon dioxide(CO_(2))exchange and crop growth in this region.The experimental site was divided into drip and flood irrigation zones.The irrigation schedules of this study aligned with the local commonly used irrigation schedule.We employed a developed chamber system to measure the diurnal CO_(2)exchange of maize plants during various growth stages under both drip and flood irrigation methods.From May to September in 2020 and 2021,two sets of repeated experiments were conducted.In each experiment,a total of nine measurements of CO_(2)exchange were performed to obtain carbon exchange data at different growth stages of maize crop.During each CO_(2)exchange measurement event,CO_(2)flux data were collected every two hours over a day-long period to capture the diurnal variations in CO_(2)exchange.During each CO_(2)exchange measurement event,the biological parameters(aboveground biomass and crop growth rate)of maize and environmental parameters(including air humidity,air temperature,precipitation,soil water content,and photosynthetically active radiation)were measured.The results indicated a V-shaped trend in net ecosystem CO_(2)exchange in daytime,reducing slowly at night,while the net assimilation rate(net primary productivity)exhibited a contrasting trend.Notably,compared with flood irrigation,drip irrigation demonstrated significantly higher average daily soil CO_(2)emission and greater average daily CO_(2)absorption by maize plants.Consequently,within the maize ecosystem,drip irrigation appeared more conducive to absorbing atmospheric CO_(2).Furthermore,drip irrigation demonstrated a faster crop growth rate and increased aboveground biomass compared with flood irrigation.A strong linear relationship existed between leaf area index and light utilization efficiency,irrespective of the irrigation method.Notably,drip irrigation displayed superior light use efficiency compared with flood irrigation.The final yield results corroborated these findings,indicating that drip irrigation yielded higher harvest index and overall yield than flood irrigation.The results of this study provide a basis for the selection of optimal irrigation methods commonly used in the Hetao Irrigation District.This research also serves as a reference for future irrigation studies that consider measurements of both carbon emissions and yield simultaneously.

Study on Relationship Between Arc Behavior and Joint Forming in Cold Metal Transfer Welding Technology with Polarity⁃Exchanging

Cold metal transfer with polarity⁃exchanging is a new integrated welding technology based on MIG.Due to the alternation of the positive and negative polarities of the wire,favorable control upon the deposition rate and the welding shape coefficient was obtained in order to meet the desired joint design,and the related controlling principles and joint characteristics were reported.Droplet transfer physical behavior exhibited strong dependability on the studied welding parameters,such as welding voltage,welding current,wire feeding speed,and polarity⁃exchanging.This welding technology provides a new way for the welding of body⁃in⁃white(BIW)thin sheet with special demands.Moreover,the typical quality defects of MIG were greatly improved.Our study provides important technical information from the perspective of industrial application of MIG and sheds light on the higher application level of MIG in BIW welding.

Influence of initial check, information exchange, final accuracy check, reaction information nursing on the psychology of elderly with lung cancer

BACKGROUND As one of the fatal diseases with high incidence,lung cancer has seriously endangered public health and safety.Elderly patients usually have poor self-care and are more likely to show a series of psychological problems.AIM To investigate the effectiveness of the initial check,information exchange,final accuracy check,reaction(IIFAR)information care model on the mental health status of elderly patients with lung cancer.METHODS This study is a single-centre study.We randomly recruited 60 elderly patients with lung cancer who attended our hospital from January 2021 to January 2022.These elderly patients with lung cancer were randomly divided into two groups,with the control group taking the conventional propaganda and education and the observation group taking the IIFAR information care model based on the conventional care protocol.The differences in psychological distress,anxiety and depression,life quality,fatigue,and the locus of control in psychology were compared between these two groups,and the causes of psychological distress were analyzed.RESULTS After the intervention,Distress Thermometer,Hospital Anxiety and Depression Scale(HADS)for anxiety and the HADS for depression,Revised Piper’s Fatigue Scale,and Chance Health Locus of Control scores were lower in the observation group compared to the pre-intervention period in the same group and were significantly lower in the observation group compared to those of the control group(P<0.05).After the intervention,Quality of Life Questionnaire Core 30(QLQ-C30),Internal Health Locus of Control,and Powerful Others Health Locus of Control scores were significantly higher in the observation and the control groups compared to the pre-intervention period in their same group,and QLQ-C30 scores were significantly higher in the observation group compared to those of the control group(P<0.05).CONCLUSION The IIFAR information care model can help elderly patients with lung cancer by reducing their anxiety and depression,psychological distress,and fatigue,improving their tendencies on the locus of control in psychology,and enhancing their life qualities.

Heat Exchanging Process of Metal Target under Millisecond Pulsed Laser Shocking

Laser plays an important role in synthesizing nanometer material. A three-dimensional mathematical model is established in this paper when single pulsed millisecond laser shocks the surface of the metal target at a liquid-solid interface. By changing laser power density and target size, the temperature field variation of the metal target is investigated. Results show that the generation process of nanoparticles includes heating, melting and boiloff.

Primary Study of Power-Exchanging Trans- action Model Among Price-Varied Power Plants in Practical Conditions

At present, electricity price to grid of domestic power plants is priced by the national administration based on the policy of "one power plant with one electricity price to grid," which is difficult to realize real bidding for access to grid in practice in a short term. This paper presents one kind of power-exchanging transaction model among price-varied power plants, which will be beneficial to price-varied power plants without any loss of profits of them and guarantee state-owned assets profits in minimum loss with no promotion of average price limit by power plants. Under ideal conditions, the computation results showed the sufficiency and necessity of power-exchanging transaction and maximum similarity with the requirements of optimized resources disposition in economics. The presented model is shown to be full of practicability and has been used in some part of power market.

Spin wave resonance frequency in bilayer ferromagnetic films with the biquadratic exchange interaction

The dependences of spin wave resonance(SWR)frequency on the surface anisotropy field,interface exchange coupling,symmetry,biquadratic exchange(BQE)interaction,film thickness,and the external magnetic field in bilayer ferromagnetic films are theoretically analyzed by employing the linear spin wave approximation and Green’s function method.A remarkable increase of SWR frequency,except for energetically lower two modes,can be obtained in our model that takes the BQE interaction into account.Again,the effect of the external magnetic field on SWR frequency can be increased by increasing the biquadratic to interlayer exchange ratio.It has been identified that the BQE interaction is of utmost importance in improving the SWR frequency of the bilayer ferromagnetic films.In addition,for bilayer ferromagnetic films,the frequency gap between the energetically highest mode and lowest mode is found to increase by increasing the biquadratic to interlayer exchange ratio and film thickness and destroying the symmetry of the system.These results can be used to improve the understanding of magnetic properties in bilayer ferromagnetic films and thus may have prominent implications for future magnetic devices.

Enhanced Mechanism for Link Failure Rerouting in Software-Defined Exchange Point Networks

Internet Exchange Point(IXP)is a system that increases network bandwidth performance.Internet exchange points facilitate interconnection among network providers,including Internet Service Providers(ISPs)andContent Delivery Providers(CDNs).To improve service management,Internet exchange point providers have adopted the Software Defined Network(SDN)paradigm.This implementation is known as a Software-Defined Exchange Point(SDX).It improves network providers’operations and management.However,performance issues still exist,particularly with multi-hop topologies.These issues include switch memory costs,packet processing latency,and link failure recovery delays.The paper proposes Enhanced Link Failure Rerouting(ELFR),an improved mechanism for rerouting link failures in software-defined exchange point networks.The proposed mechanism aims to minimize packet processing time for fast link failure recovery and enhance path calculation efficiency while reducing switch storage overhead by exploiting the Programming Protocol-independent Packet Processors(P4)features.The paper presents the proposed mechanisms’efficiency by utilizing advanced algorithms and demonstrating improved performance in packet processing speed,path calculation effectiveness,and switch storage management compared to current mechanisms.The proposed mechanism shows significant improvements,leading to a 37.5%decrease in Recovery Time(RT)and a 33.33%decrease in both Calculation Time(CT)and Computational Overhead(CO)when compared to current mechanisms.The study highlights the effectiveness and resource efficiency of the proposed mechanism in effectively resolving crucial issues inmulti-hop software-defined exchange point networks.

Petrology of Spinel-Lherzolite Xenoliths from Mazéléand Others Northen Xenoliths Localities of Cameroon Volcanic Line: Exchange Reactions and Equilibrium State

The alkaline volcanism of the Cameroon Volcanic Line in its northern domain has raised many fresh enclaves of peridotites. The samples selected come from five (05) different localities (Liri, in the plateau of Kapsiki, Mazélé in the NE of Ngaoundéré, Tello and Ganguiré in the SE of Ngaoundéré and Likok, locality located in the west of Ngaoundé). The peridotite enclaves of the above localities show restricted mineralogical variation. Most are four-phase spinel-lherzolites, indicating that this is the main lithology that forms the lithospheric mantle below the shallow zone. No traces of garnet or primary plagioclase were detected, which strongly limits the depth range from which the rock fragments were sampled. The textures and the wide equilibrium temperatures (884˚C - 1115˚C) indicate also entrainment of lherzolite xenoliths from shallow depths within the lithosphere and the presence of mantle diapirism. The exchange reactions and equilibrium state established in this work make it possible to characterize the chemical composition of the upper mantle of each region and test the equilibrium state of the phases between them. Variations of major oxides and incompatible elemental concentrations in clinopyroxene indicate a primary control by partial melting. The absence of typical “metasomatic” minerals, low equilibration temperatures and enriched LREE patterns indicate that the upper mantle below septentrional crust of Cameroun underwent an event of cryptic metasomatic enrichment prior to partial melting. The distinctive chemical features, LREE enrichment, strong U, Ce and Pr, depletion relative to Ba, Nb, La, Pb, and T, fractionation of Zr and Hf and therefore ligh high Zr/Hf ratio, low La/Yb, Nb/La and Ti/Eu are all results of interaction of refractory peridotite residues with carbonatite melts.

Towards high-performance and robust anion exchange membranes(AEMs)for water electrolysis:Super-acid-catalyzed synthesis of AEMs

The increasing demand for hydrogen energy to address environmental issues and achieve carbon neutrality has elevated interest in green hydrogen production,which does not rely on fossil fuels.Among various hydrogen production technologies,anion exchange membrane water electrolyzer(AEMWE)has emerged as a next-generation technology known for its high hydrogen production efficiency and its ability to use non-metal catalysts.However,this technology faces significant challenges,particularly in terms of the membrane durability and low ionic conductivity.To address these challenges,research efforts have focused on developing membranes with a new backbone structure and anion exchange groups to enhance durability and ionic conductivity.Notably,the super-acid-catalyzed condensation(SACC)synthesis method stands out due to its user convenience,the ability to create high molecular weight(MW)polymers,and the use of oxygen-tolerant organic catalysts.Although the synthesis of anion exchange membranes(AEMs)using the SACC method began in 2015,and despite growing interest in this synthesis approach,there remains a scarcity of review papers focusing on AEMs synthesized using the SACC method.The review covers the basics of SACC synthesis,presents various polymers synthesized using this method,and summarizes the development of these polymers,particularly their building blocks including aryl,ketone,and anion exchange groups.We systematically describe the effects of changes in the molecular structure of each polymer component,conducted by various research groups,on the mechanical properties,conductivity,and operational stability of the membrane.This review will provide insights into the development of AEMs with superior performance and operational stability suitable for water electrolysis applications.

Influence of Al_(2)O_(3)/SiO_(2) Ratio on the Structure and Properties of Na^(+)/K^(+)Ion Exchange Na_(2)O-MgO-Al_(2)O_(3)-SiO_(2) Glasses

In this work,the structure,viscosity and ion-exchange process of Na_(2)O-MgO-Al_(2)O_(3)-SiO_(2) glasses with different Al_(2)O_(3)/SiO_(2) molar ratios were investigated.The results showed that,with increasing Al_(2)O_(3)/SiO_(2) ratio,the simple structural units Q_(1) and Q_(2) transformed into highly aggregated structural units Q_(3) and Q_(4),indicating the increase of polymerization degree of glass network.Meanwhile,the coefficient of thermal expansion decreased from 9.23×10^(-6)℃^(-1) to 8.88×10^(-6)℃^(-1).The characteristic temperatures such as melting,forming,softening and glass transition temperatures increased with the increase of Al_(2)O_(3)/SiO_(2) ratio,while the glasses working temperature range became narrow.The increasing Al_(2)O_(3)/SiO_(2) ratio and prolonging ion-exchange time enhanced the surface compressive stress(CS)and depth of stress layer(DOL).However,the increase of ion exchange temperature increased the DOL and decreased the CS affected by stress relaxation.There was a good linear relationship between stress relaxation and surface compressive stress.Chemical strengthening significantly improved the hardness of glasses,which reached the maximum value of(622.1±10)MPa for sample with Al_(2)O_(3)/SiO_(2) ratio of 0.27 after heat treated at 410℃for 2 h.