目的研究遗传性视网膜色素变性大鼠(Royal College of Surgeon’s rat,RCS rat)在视网膜变性发展过程中是否有内源性视网膜干细胞(retinal stem cells,RSCs)激活。方法RCS-p+(视网膜含色素的变性大鼠)按出生后视网膜变性的发展状况分为...目的研究遗传性视网膜色素变性大鼠(Royal College of Surgeon’s rat,RCS rat)在视网膜变性发展过程中是否有内源性视网膜干细胞(retinal stem cells,RSCs)激活。方法RCS-p+(视网膜含色素的变性大鼠)按出生后视网膜变性的发展状况分为变性早期(RCS15d)、中期(RCS30d)、晚期(RCS90d)3个时相点,分别采用相应时期的RCS-rdy+p+(视网膜含色素的正常大鼠)作为对照组(control groups,C15d,C30d,C90d),每组4只8眼,对各组视网膜切片进行免疫荧光组织化学(抗Chx10)实验,以鉴定视网膜干细胞;进一步采用Western blot检测各组视网膜神经上皮总蛋白中Chx10表达。结果①各正常组及变性15d大鼠视网膜各层抗Chx10免疫荧光检测均为阴性,变性中、晚期视网膜光感受细胞层部位Chx10免疫荧光阳性;②Western blot检测各变性组视网膜神经上皮总蛋白中Chx10均有表达,但变性中期Chx10表达显著高于早期和晚期(P<0.05)。结论RCS大鼠变性过程中视网膜感光细胞层出现视网膜干细胞激活。展开更多
The interfaces and interface electron states in Mylar/Al/UCL/CGL/CTL multi-layered photoreceptor are discussed. The interface electron states between CGL and CTL in sample A efficiently accelerated photogeneration car...The interfaces and interface electron states in Mylar/Al/UCL/CGL/CTL multi-layered photoreceptor are discussed. The interface electron states between CGL and CTL in sample A efficiently accelerated photogeneration carrier transport processes to bring about a lower V-r value and higher light sensitivity than sample B. The process might be interpreted as a kind of assistance sensitization effect based on interfacial electron states in the high fields; that is to say, the interface electron states between CGL and CTL accelerated carriers injection from CGL into the gap states of CTL, and followed by the interface electron states modulated transport processes of carriers in the CTL.展开更多
文摘The interfaces and interface electron states in Mylar/Al/UCL/CGL/CTL multi-layered photoreceptor are discussed. The interface electron states between CGL and CTL in sample A efficiently accelerated photogeneration carrier transport processes to bring about a lower V-r value and higher light sensitivity than sample B. The process might be interpreted as a kind of assistance sensitization effect based on interfacial electron states in the high fields; that is to say, the interface electron states between CGL and CTL accelerated carriers injection from CGL into the gap states of CTL, and followed by the interface electron states modulated transport processes of carriers in the CTL.
