Biophysical warming patterns of an open-top chamber and its short-term influence on a Phragmites wetland ecosystem in China

Passive-warming, open-top chambers(OTCs) are widely applied for studying the effects of future climate warming on coastal wetlands. In this study, a set of six OTCs were established at a Phragmites wetland located in the Yellow River Delta of Dongying City, China. With data collected through online transmission and in-situ sensors, the attributes and patterns of realized OTCs warming are demonstrated.The authors also quantified the preliminary influence of experimental chamber warming on plant traits.OTCs produced an elevated average air temperature of 0.8°C(relative to controls) during the growing season(June to October) of 2018, and soil temperatures actually decreased by 0.54°C at a depth of 5 cm and 0.46°C at a depth of 30 cm in the OTCs. Variations in diel patterns of warming depend greatly on the heat sources of incoming radiation in the daytime versus soil heat flux at night. Warming effects were often larger during instantaneous analyses and influenced OTCs air temperatures from-2.5°C to 8.3°C dependent on various meteorological conditions at any given time, ranging from cooling influences from vertical heat exchange and vegetation to radiation-associated warming. Night-time temperature depressions in the OTCs were due to the low turbulence inside OTCs and changes in surface soilatmosphere heat transfer. Plant shoot density, basal diameter, and biomass of Phragmites decreased by23.2%, 6.3%, and 34.0%, respectively, under experimental warming versus controls, and plant height increased by 4.3%, reflecting less carbon allocation to stem structures as plants in the OTCs experienced simultaneous wind buffering. While these passive-warming OTCs created the desired warming effects both to the atmosphere and soils, pest damages on the plant leaves and lodging within the OTCs were extensive and serious, creating the need to consider control options for these chambers and the replicated OTCs studies underway in other Chinese Phragmites marshes(Panjin and Yancheng).

Invasive Common Reed (Phragmites australis (Cav.) Trin. ex Steud.): A Serious Ecological Threat to Ladakh Tethys Himalayan Biodiversity in Changing Climate

Ladakh’s cropped and non-cropped areas suffer greatly from weedy invasion under subsistence agricultural system where weeding is not a priority but total crop + weed biomass together contribute to livestock feeding significantly. As agriculture along with livestock rearing is major activity of livelihood options, thereby contributing significantly to Ladakh economy and income generation for supporting resource poor farmers. Amongst various invasive weed species, the common reed (Phragmites australis) has become a serious ecological threat for agri-silvi-pastoral system biodiversity and accelerated its expansion in cropped lands including many wetland and salt-affected habitats of cold arid region due to its intermediate plant growth habit of C3 - C4 photosynthetic ecotypes. This character makes Phragmites a climate-resilient species, which is a major challenge for cold arid agriculture in the changing climatic patterns. Simultaneously, it is one of the best fodder substitute available under harsh climate. This paper deals with its unique characters and potential threats to the agrobiodiversity of Ladakh.

Phragmites australis and Typha orientalis in removal of pollutant in Taihu Lake, China

Two plant populations of Phragmites australis and Typha orientalis grown in gravel and sediment substrate were studied to assess their capabilities for purifying polluted water in Taihu Lake, China. The substrate displayed most significant effects on the suspended matter （P 〈 0.01）, with the reduction of 76%-87% and 52%--63% for P. australis, and 83%-86% and 45%-62% for T. orientalis in gravel substrate and sediment substrate, respectively. Both species and substrates significantly decreased the N and P concentrations of water body （P 〈 0.01）. P. australis showed higher total N and P concentrations in tissues than T. orientalis and had a greater potential to remove nutrients from the lake. Phosphate was easily to concentrate in the belowground tissues, while nitrate concentration was higher in leaf and stalk. Therefore, harvesting the aboveground tissues could take most of nitrate out of the sediment. The saturate photosynthetic rate （Asat） of P. australis was higher than that of T. orientalis when grown in sediment substrate. But instance water-use- efficiency （WUEi） （A/E） and intrinsic water use efficiency （A/gs） showed the maximum values of two species grown in river water. With significant difference in gs, however, intercellular CO2 concentration （Ci） had no obvious difference in two species which indicated that high Asat value of P. australis might result from the increased carboxylation capacity of the mesophyll, because of the central role of N in photosynthetic enzymes. Our findings suggest that the plants could absorb most of nitrogen in polluted water, while gravel displayed a high capacity for absorbing the suspended matters and phosphate salts. Therefore, biological and physiological pathways for pollutant removal should be integrated.

Emissions of Biogenic Sulfur Gases(H2S,COS)from Phragmites australis Coastal Marsh in the Yellow River Estuary of China

Emissions of biogenic sulfur gases(hydrogen sulfide(H_2S) and carbonyl sulfide(COS)) from Phragmites australis coastal marsh in the Yellow River estuary of China were determined during April to December in 2014 using static chamber-gas chromatography technique with monthly sampling. The results showed that the fluxes of H_2S and COS both had distinct seasonal and diurnal variations. The H_2S fluxes ranged from 0.09 μg/(m^2·h) to 7.65 μg/(m^2·h), and the COS fluxes ranged from –1.10 μg/(m^2·h) to 3.32 μg/(m^2·h). The mean fluxes of H_2S and COS from the P. australis coastal marsh were 2.28 μg/(m^2·h), and 1.05 μg/(m^2·h), respectively. The P. australis coastal marsh was the emission source of both H_2S and COS over the whole year. Fluxes of H_2S and COS were both higher in plant growing season than in the non-growing season. Temperature had a dramatic effect on the H_2S emission flux, while the correlations between COS flux and the environmental factors were not found during sampling periods. More in-depth and comprehensive research on other related factors, such as vegetation, sediment substrates, and tidal action is needed to discover and further understand the key factors and the release mechanism of sulfur gases.

The Phragmites Root-Inhabiting Microbiome: A Critical Review on Its Composition and Environmental Application

As widespread wetland plants,Phragmites play a vital role in water purification and are widely utilized in constructed wetlands(accounting for 15.5%of applied wetland plants)as a natural alternative to wastewater treatment.However,despite such common applications,current understanding of the basic composition of the Phragmites root-inhabiting microbiome and the complex functions of each member of this microbiome remains incomplete,especially regarding pollution remediation.This review summa-rizes the advances that have been made in ecological and biochemical research on the Phragmites root microbiome,including bacteria,archaea,and fungi.Based on next-generation sequencing,microbial com-munity compositions have been profiled under various environmental conditions.Furthermore,culture-based methods have helped to clarify the functions of the microbiome,such as metal iron stabilization,organic matter degradation,and nutrient element transformation.The unique community structure and functions are highly impacted by Phragmites lineages and environmental factors such as salinity.Based on the current understanding of the Phragmites root microbiome,we propose that synthetic microbial com-munities and iron–manganese plaque could be applied and intensified in constructed wetlands to help promote their water purification performance.

The adaptive significance of differences of root morphology, anatomy and physiology from three ecotypes of reed(Phragmites communis Trin.)

Reeds are widely distributed in drought and high salt conditions of northwestern China. Leaf epidermal micromorphology, anatomy, chloroplast ultrastructure and physio-chemical characteristics due to long-term adaptation in the natural habitats of common reed （Phragmites communis Trin.） contrasted considerably among three different ecotypes： dune reed （DR）, Gobi salt reed （GSR） and swamp reed （SR）. The main objective of the present study is to determine the adapting characteristics of morphology, anatomy and physiological responses of thin roots in DR, GSR and SR. The results show that root length density was higher in SR and few root hairs were observed in DR. Cross-section anatomical features show that each ecotype has an endodermis and exodermis, while cortex thickness and proportion of root cortical aerenchyma and stele in root structure varied among the three ecotypes. The stele and xylem share a larger area in DR compared to GSR and SR. GSR has a large proportion of the cortex with radialized distribution of aerenchyma cells spacing, and the cortex has a peripheral, mechanically stiff ring in the exodermis. SEM and TEM microscope images show that GSR has a scle- renchyma ring with high lignification in the exodermis. The physio-chemical parameters show that GSR had a higher level of stress tolerance than DR. These findings indicate that developed water-absorbing tissues were largely distributed in the root structure of DR, and a main framework with supporting function spacing with aerenchyma was dominant in GSR in the long term adaptation to their natural habitats, respectively.

石羊河中游河岸芦苇(Phragmites australis)群落空间格局

以石羊河中游典型河岸植物芦苇（Phragmites australis）为对象,采用点格局分析法研究了以芦苇为优势种或伴生种群落中芦苇的空间分布格局特征,并对芦苇和沙蒿（Artemisia desertorum）、梭梭（Haloxylon ammodendron）、变异黄耆（Astragalus variabilis）及蓼子朴（Inula salsoloides）的种间关联性进行了分析,揭示了芦苇的空间分布格局和种间关联性与河岸距离的关系。结果表明：在5m的一系列空间尺度上,以芦苇为优势种的群落中,距河岸近的芦苇呈随机分布,而距河岸远的芦苇在小尺度上为集群分布,较大尺度上变为随机分布;以芦苇为伴生种的群落中,芦苇均表现出集群分布特征,且距离河岸近的芦苇聚集程度大于距离河岸远的芦苇。芦苇和沙蒿在较大尺度上具有正关联关系,距离河岸越远,芦苇和沙蒿呈正关联的尺度越小,关联程度越强。芦苇和变异黄耆在4.1～5m尺度上具有负关联关系,和蓼子朴在1.5～4.6m尺度上具有正关联关系。

Structural effects of reedbed grazing and its cessation on reed-nesting songbird densities

Reedbeds are crucial breeding habitats for vulnerable songbird species.Irrespective of their protection status,these habitats may be threatened by organic matter accumulation,progressively leading to structural homoge-nization and habitat succession towards woodland.Managers prevent excessive litter build-up with various in-terventions opening-up the reedbeds,such as grazing,which may be detrimental or suitable for some reed bird species.We assessed the effects of extensive grazing by horses and its cessation in the medium and long terms on reedbed structure,and the consequences on reed-nesting songbird densities in Estagnol Nature Reserve,a pro-tected wetland near the French Mediterranean coast.We compared reedbed structural features between grazed,newly ungrazed and old-ungrazed plots.During nine years,we censused four songbird species in spring and collected water level data in the same survey plots.Grazing reduced reedbed extent,rejuvenated the vegetation with more short green stems on a thinner litter,and produced higher structural heterogeneity and discontinuity compared to long-lasting non grazing.Newly ungrazed plot showed intermediate effects.All surveyed songbird total densities were similar among plots while species numbers and densities differed.Grazed reedbed was more attractive to Moustached Warblers(Acrocephalus melanopogon)and Great Reed Warblers(Acrocephalus arundi-naceus),likely due to the large edges and the high vegetation structural heterogeneity.However,Moustached Warblers were more negatively affected by higher water level in grazed reedbed,presumably because flooding prevents litter foraging.The newly ungrazed reedbed was not preferred by any species.All warbler species were found under low density in the old-ungrazed reedbed,where Reed Buntings(Emberiza schoeniclus)were exclu-sively found.Food availability related to thick litter layer may explain this predilection.Common Reed Warblers(Acrocephalus scirpaceus)were found everywhere in similar densities.We conclude that reedbed management by extensive horse grazing and also its long-term cessation may benefit several songbird species.Within a context of wetland degradation and disappearance,both management strategies are useful to establish and maintain suitable habitats for reed-nesting songbird communities.

Performance of a Horizontal Flow Constructed Reed Bed Filter for Municipal Wastewater Treatment: The Case Study of the Prototype Installed at Gaston Berger University, Saint-Louis, Senegal

In Saint-Louis, Senegal, a constructed wetland with horizontal flow reed beds (FHa and FHb) has demonstrated significant efficacy in treating municipal wastewater. Analyzing various treatment stages, the system showed only a slight temperature variation, from an influent average of 26.3°C to an effluent of 24.7°C. Electrical conductivity decreased from 1331 mS/cm to 974.5 mS/cm post-primary treatment, with suspended solids (SS) dramatically reduced from 718.9 mg/L to 5.7 mg/L in the final effluent. Biochemical oxygen demand (BOD5) and chemical oxygen demand (COD) saw a notable decrease, from initial levels of 655.6 mg/L and 1240 mg/L to 2.3 mg/L and 71.3 mg/L, respectively. Nitrogenous compounds (N-TN) and phosphates () also decreased significantly, indicating the system’s nutrient removal capacity. Microbiological analysis revealed a reduction in fecal coliforms from 7.5 Ulog/100ml to 1.8 Ulog/100ml and a complete elimination of helminth eggs. The presence of Phragmites and Typha was instrumental in enhancing these reductions. The system’s compliance with the Senegalese standards for disposal into natural environments, WHO recommendations for unrestricted water reuse in irrigation, and the European legislation for water reuse was established. The effluent quality met the stringent criteria for various classes of agricultural reuse, illustrating the system’s potential for sustainable water management. This wetland model presents a robust solution for water-stressed regions, ensuring environmental protection while supporting agricultural needs. The study calls for ongoing research to further refine the system for optimal, reliable wastewater treatment and water resource sustainability.

河西走廊沙地芦苇(Phragmites australis)根际土壤微生物群落多样性

以河西走廊绿洲-荒漠过渡带不同固定程度的沙地生境(半固定沙丘迎风坡、半固定沙丘背风坡、固定沙丘和丘间平地)生长的芦苇(Phragmites australis)为研究对象,应用高通量测序技术,对芦苇根际土壤细菌和真菌群落的结构和多样性特征进行了分析比较。结果表明:4种沙地生境芦苇根际土壤微生物的群落多样性存在较明显的差异,细菌和真菌群落的OTU(操作分类单元)数量均为固定沙丘最高。变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)和酸杆菌门(Acidobacteria)为芦苇根际土壤中主要的优势细菌菌群,其中变形菌门和放线菌门在4种沙地类型中的相对丰度最高;真菌群落中的优势菌群为子囊菌门(Ascomycota)和担子菌门(Basideiomycota),其中子囊菌门为主要优势菌门。不同沙地生境芦苇根际土壤的理化性质存在显著差异,有机质、碱解氮和速效磷含量均随着沙丘固定程度的增加呈增大趋势,均为丘间平地最高,固定沙丘次之,背风坡较低,迎风坡最小;土壤pH值及有机质、碱解氮和速效磷含量对芦苇根际土壤细菌和真菌群落的多样性存在较显著的影响(P<0.05)。

Growth and nutrient accumulation of Phragmites australis in relation to water level variation and nutrient loadings in a shallow lake

Shallow lake eutrophication is a global environmental issue. This study investigated the effects of water level variation and nutrient loadings on the growth and nutrient accumulation of Phragmites australis （reed） by field samplings in Baiyangdian Lake, the largest shallow lake of northern China. The field samplings were conducted in two sites of different nutrient loadings during the whole growth period of reeds, and three types of zones with different water depths were chosen for each site, including the terrestrial zone with water level below the ground, the ecotone zone with the water level varying from belowground to aboveground, and the submerged zone with water level above the ground. The result showed that reed growth was more limited by water level variation than nutrient loadings. The average stem lengths and diameters in terrestrial zones were about 26.3%-27.5% and 7.2%-12.0% higher than those in submerged zones, respectively. Similarly, the terrestrial status increased the aboveground biomass of reeds by 36.6%-51.8% compared with the submerged status. Both the nutrient concentrations and storages in the aboveground reeds were mainly influenced by the nutrient loadings in surface water and sediment rather than the water level variation of the reed growth environment, and the nutrient storages reached their maxima in late August or early September. It was observed that the maximum nitrogen storage occurred in the terrestrial zone with higher nutrient loadings, with the value of 74.5 g/m2. This study suggested that water level variation and nutrient loadings should be considered when using reeds to control and remediate eutrophication of shallow lakes.

PcTGD,a highly expressed gene in stem,is related to water stress in reed (Phragmites communis Trin.)

To compare differential gene expression among three ecotypes of reed (Phragmites communis Trin.), dune reed (DR), heavy-salt meadow reed (HSR) and light-salt meadow reed (LSR), mRNA transcripts were displayed by cDNA-AFLP (amplified fragment length polymorphisms). The result revealed that a relatively small number of genes are likely involved in adaptations of DR and HSR to stresses. A full-length cDNA encoding dTDP-D-glucose dehydratase gene (PcTGD) was subsequently cloned from DR. Northern blot analysis showed that it is highly expressed in stem as well as rhizoma of the three ecotypes. However, its expression in DR stem was much higher than that of the other two ecotypes. After the removal of water stress, the expression of PcTGD was significantly reduced, suggesting that it possibly plays a role in adaptation of DR to water stress through an osmotic regulation mechanism.

Novel compound specified in dune reed (Phragmites communis Trin.) and its possible role as a protectant on the chloroplasts under stress

A small solute has been obtained with column chromatography, which only exists and accumulates in the leaves of dune reed in northwest China’s desert region. Its unique polyamine and aromatic features are quite different from those well-known ones accumulated under stresses. The natural quantity of the solute is strikingly proportional to the level of local high temperature, and a large section is distributed in the photosynthetic chloroplasts. Under lab conditions, we have proved that the solute plays a role in protecting or conserving chloroplasts under heat stress. So it is reasonable to suppose that the high resistance of dune reed to environmental high temperature and/or drought is at least partially due to the accumulation of this unique solute.

Dynamic Response of Phragmites australis and Suaeda salsa to Climate Change in the Liaohe Delta Wetland

Because of its unique geographical location and ecological function, the Liaohe Delta Wetland is important in maintaining regional ecological balance and security. Monitoring and evaluating changes in the wetland are therefore of great importance. We used medium-and high-resolution satellite data, meteorological station data, and site measurement data to analyze changes in the area and spatial distribution of Phragmites australis and Suaeda salsa in the Liaohe Delta Wetland from 1998 to 2017, as well as their growth response to the climate change. The results showed that during 1998–2017, the areas of both P. australis and S. salsa wetlands alternated through periods of decreasing,increasing, and then decreasing trends. The annual change in the area and spatial distribution range of S. salsa fluctuated more than that of P. australis. The annual variation of normalized difference vegetation index(NDVI) in P. australis wetland showed an upward trend from 1998 to 2017. The area of P. australis cover that was improved, unchanged, and decreased accounted for 81.8%, 12.3%, and 5.9%, respectively, of the total area;evaporation and wind speed were the main meteorological factors affecting the NDVI;and contribution rates of the climate change and human activities to the NDVI were 73.2% and 26.8%, respectively. The area with vegetation cover being mainly S.salsa that was improved, unchanged, and decreased accounted for 63.3%, 18.3%, and 18.4%, respectively, of the total area;and no meteorological factors significantly affected the NDVI of S. salsa in the region. The interaction between vegetation growth and meteorological factors may help to explain the increasing trend in vegetation cover.The improvement in wetland vegetation also led to carbon sequestration and an increase in sequestration capacity.

Effect of Planting Condition and Time on Survival Rate and Growth of Phragmites australis

The field trial was established to investigate the effects of planting condition(soil water content,soil buried depth,rhizome length)and time on the survival rate and growth of Phragmites australis rhizomes.The results indicated that survival rate and growth of Phragmites australis were affected by soil water content and rhizome length significantly,but not by soil buried depth.The survival rate of Phragmites australis in moist condition was higher than those in natural and flooding conditions.Rhizomes length did not affect survival rate,height and shoot number,but influenced biomass and buds number,which were higher with 15 cm length than those with 30 cm length.Based on the suitable soil water content(moisture)and rhizome length(15 cm),the survival rate of Phragmites australis was the highest when they were planted in May(91%)comparing to June and July.The number of shoots and buds were the highest in June,which had more suitable temperature and light for Phragmites australis to grow.Therefore,the optimizing transplanting condition of Phragmites australis was that rhizomes of 15 cm with some buds were planted in May with moisture(soil water content).

Effect of Groundwater Level Fluctuation on Phragmites australis Evapotranspiration in the Baiyangdian Lake

Abstract:Hydrological regimes influence ecological patterns and processes as well as alter rates of wetland evapotranspiration.This study aimed to investigate the impact of groundwater fluctuation on evapotranspiration of Phragmites australis.Supported by field data obtained from the Baiyangdian Lake in northern China,the variations in groundwater levels were explored,and the changes in soil water and evapotranspiration of reed were analyzed to investigate different groundwater level scenarios using HYDRUS-1D model.The results showed that soil water content,recharged by groundwater,remained stable in the lower soil layer but varied strongly in the upper layer of the soil profile;in comparison to evaporation,Phragmites australis transpiration contributed significantly more to the overall evapotranspiration rate;the high levels of evapotranspiration could be maintained when groundwater levels vary between 1.0 m and 1.8 m,while it was reduced with an increase in groundwater levels as a result of water stress conditions.The results also indicated that the evapotranspiration of Phragmites australis could maintain higher evapotranspiration rates under natural water levels.The evapotranspiration,in other words,might be the main water consumer,but it nevertheless has little effect on water levels during water shortages.The evapotranspiration of Phragmites australis responded to the changes in groundwater levels could help researchers understand water requirements of the wetlands and establish suitable water levels for the wetlands.

Decomposition of Phragmites australis rhizomes in artificial land-water transitional zones （ALWTZs） and management implications

Rhizomes are essential organs for growth and expansion of Phragmites australis. They function as an important source of organic matter and as a nutrient source, especially in the artificial land-water transitional zones （ALWTZs） of shallow lakes. In this study, decomposition experiments on 1- to 6-year-old R australis rhizomes were conducted in the ALWTZ of Lake Baiyangdian to evaluate the contribution of the rhizomes to organic matter accumulation and nutrient release. Mass loss and changes in nutrient content were measured after 3, 7, 15, 30, 60, 90, 120, and 180 days. The decomposition process was modeled with a composite exponential model. The Pearson correlation analysis was used to analyze the relationships between mass loss and litter quality factors. A multiple stepwise regression model was utilized to determine the dominant factors that affect mass loss. Results showed that the decomposition rates in water were significantly higher than those in soil for 1- to 6-year-old rhizomes. However, the sequence of decomposition rates was identical in both water and soil. Significant relationships between mass loss and litter quality factors were observed at a later stage, and P-related factors proved to have a more significant impact than N-related factors on mass loss. According to multiple stepwise models, the C/P ratio was found to be the dominant factor affecting the mass loss in water, and the C/N and C/P ratios were the main factors affecting the mass loss in soil. The combined effects of harvesting, ditch broadening, and control of water depth should be considered for lake administrators.

Synchrotron micro-scale measurement of metal distributions in Phragmites australis and Typha latifolia root tissue from an urban brownfield site

Liberty State Park in New Jersey,USA,is a ＂brownfield＂ site containing various levels of contaminants.To investigate metal uptake and distributions in plants on the brownfield site,Phragmites australis and Typha latifolia were collected in Liberty State Park during the growing season（May–September）in 2011 at two sites with the high and low metal loads,respectively.The objective of this study was to understand the metal（Fe,Mn,Cu,Pb and Zn）concentration and spatial distributions in P.australis and T.latifolia root systems with micro-meter scale resolution using synchrotron X-ray microfluorescence（μXRF）and synchrotron X-ray computed microtomography（μCMT）techniques.The root structure measurement by synchrotron μCMT showed that high X-ray attenuation substance appeared in the epidermis.Synchrotron μXRF measurement showed that metal concentrations and distributions in the root cross-section between epidermis and vascular tissue were statistically different.Significant correlations were found between metals（Cu,Mn,Pb and Zn）and Fe in the epidermis,implying that metals were scavenged by Fe oxides.The results from this study suggest that the expression of metal transport and accumulation within the root systems may be element specific.The information derived from this study can improve our current knowledge of the wetland plant ecological function in brownfield remediation.

中国东北松嫩平原丹顶鹤繁殖种群调查(英文)

自2004年至2008年,在白鹤GEF项目的支持下,全国鸟类环志中心于每年5月在东北松嫩平原的4个国家级自然保护区,即黑龙江的扎龙国家级自然保护区、内蒙古的科尔沁国家级自然保护区、吉林的向海和莫莫格国家级自然保护区,开展了繁殖水鸟的地面调查。调查的目的在于了解各个保护区繁殖水鸟的分布和种群动态。丹顶鹤(Grus japonensis)是本调查活动的重要目标物种。通过5年的调查,了解到松嫩平原的扎龙保护区是目前丹顶鹤最为重要的繁殖地,逾90%的繁殖丹顶鹤种群分布在扎龙保护区,但种群数量变动较大,变动幅度介于112–275只之间。良好的芦苇(Phragmites australis)生境是丹顶鹤的繁殖种群保持稳定和增长的首要条件。通过给湿地供水可以缓解丹顶鹤繁殖栖息地的快速退化,但科学合理的供水机制是保证丹顶鹤繁殖成功的前提。

蒸气爆破预处理芦苇酶解工艺优化及产物分析

芦苇（Phragmites australis）是一种有潜力的能源作物,为优化芦苇酶解糖化工艺,应用Plackett-Burman试验设计筛选影响芦苇酶糖化的重要参数,通过Box-Behnken设计确定重要参数的最佳水平,应用高效液相色谱仪（HPLC）对糖化过程中的单糖种类及含量进行分析.结果表明,影响芦苇酶糖化的重要参数是H2SO4浓度、Tween-80和MnSO4浓度,最佳工艺参数为H2SO4浓度0.88%、表面活性剂Tween-80添加量0.61%、MnSO4添加量0.26%,在此条件下,8～10 h可以完成糖化,总还原糖浓度达到45.68 mg/mL,同时验证了数学模型的有效性, 液相分析表明糖化液中主要的糖种类为葡萄糖和木糖, 其含量分别为21.36、16.62 mg/mL,阿拉伯糖、纤维二糖和半乳糖含量较少.