[Objectives]The study was to identify the casual agent of freckle disease on Cavendish banana in Hainan Province,China.[Methods]Fungal isolates were isolated from affected leaf tissues and identified by the morphologi...[Objectives]The study was to identify the casual agent of freckle disease on Cavendish banana in Hainan Province,China.[Methods]Fungal isolates were isolated from affected leaf tissues and identified by the morphological features,molecular identification and pathogenicity test.[Results]The fungus isolated from affected leaf tissues was identified as Phyllosticta capitalensis based on the morphological properties of the colony and spore,coupled with sequence analyses of the internal transcribed spacer(ITS)region and the large subunit(LSU)rDNA gene.Koch s postulates were fulfilled by successfully re-isolating the pathogen from the artificial inoculated leaves.[Conclusions]P.capitalensis is a new pathogen responsible for Cavendish banana freckle disease in Hainan.展开更多
[Objectives]This study was conducted to clarify the biological characteristics of the pathogen Phyllosticta capitalensis,the causal agent of freckle disease on Cavendish banana in Hainan Province,China.[Methods]The im...[Objectives]This study was conducted to clarify the biological characteristics of the pathogen Phyllosticta capitalensis,the causal agent of freckle disease on Cavendish banana in Hainan Province,China.[Methods]The impact of various nutritional and environmental factors,including media,carbon sources,nitrogen sources,temperature,pH and light on the growth and sporulation of P.capitalensis was assessed using two distinct methods:mycelium growth rate and blood counting chamber.[Results]The mycelial growth and sporulation of P.capitalensis on different media exhibited notable differences.The use of banana leaf extract dextrose agar(BLEAD)and carrot agar(CA)was observed to facilitate rapid mycelial growth.The potato dextrose agar(PDA)and potato sucrose agar(PSA)were conducive to the production of conidia.The utilization of distinct carbon and nitrogen sources exerted a pronounced influence on the growth of P.capitalensis.Maltose,dextrose,fructose,and casein acid hydrolysate were the preferred substrates for mycelial growth.The tested carbon and nitrogen sources did not significantly stimulate conidial production,whereas dextrose and NaNO 3 were found to favor sporulation.The optimal temperature for mycelial growth and conidial production was determined to be 28 and 32℃,respectively.No mycelial growth was observed at 5℃.Active mycelial growth was observed at pH 6-10,with pH 6-7 being particularly conducive to sporulation.Complete darkness was conducive to mycelial growth and sporulation.[Conclusions]It is recommended that BLEDA and PDA should be incubated at 28℃for 14 d in the dark for the purpose of mycelial growth and sporulation of P.capitalensis,respectively.展开更多
The identity of the casual agent of freckle disease of banana was investigated.The pathogen is generally referred to in literature under its teleomorphic name,Guignardia musae,or that of its purported anamorph,Phyllos...The identity of the casual agent of freckle disease of banana was investigated.The pathogen is generally referred to in literature under its teleomorphic name,Guignardia musae,or that of its purported anamorph,Phyllosticta musarum.Based on morphological and molecular data from a global set of banana specimens,several species were found associated with freckle disease.Phyllosticta maculata(from Southeast Asia and Oceania)is introduced as a new name for Guignardia musae,and an epitype is designated from Australia.Phyllosticta musarum(from India and Thailand)is shown to represent a distinct species,and the name is fixed by designation of an epitype from India.Guignardia stevensii is confirmed as distinct species from Hawaii,while Guignardia musicola from northern Thailand is shown to contain different taxa and is regarded as nomen confusum.Phyllosticta cavendishii is described as a new,widely distributed species,appearing primarily on Cavendish,but also on non-Cavendish banana cultivars.展开更多
琯溪蜜柚黑斑病(pomelo black spot)的病原菌为亚洲柑橘叶点霉Phyllosticta citriasiana。田间系统观察和病原菌侵染试验结果表明,蜜柚黑斑病是一种潜伏侵染病害,上年度被侵染的带菌叶片为次年病害的侵染源,蜜柚花期至幼果期是病菌侵染...琯溪蜜柚黑斑病(pomelo black spot)的病原菌为亚洲柑橘叶点霉Phyllosticta citriasiana。田间系统观察和病原菌侵染试验结果表明,蜜柚黑斑病是一种潜伏侵染病害,上年度被侵染的带菌叶片为次年病害的侵染源,蜜柚花期至幼果期是病菌侵染盛期和病害防治适期。如果错过这两个时期,将难以达到理想的防治效果。展开更多
基金Supported by Hainan Provincial Natural Science Foundation of China(322MS114).
文摘[Objectives]The study was to identify the casual agent of freckle disease on Cavendish banana in Hainan Province,China.[Methods]Fungal isolates were isolated from affected leaf tissues and identified by the morphological features,molecular identification and pathogenicity test.[Results]The fungus isolated from affected leaf tissues was identified as Phyllosticta capitalensis based on the morphological properties of the colony and spore,coupled with sequence analyses of the internal transcribed spacer(ITS)region and the large subunit(LSU)rDNA gene.Koch s postulates were fulfilled by successfully re-isolating the pathogen from the artificial inoculated leaves.[Conclusions]P.capitalensis is a new pathogen responsible for Cavendish banana freckle disease in Hainan.
基金Supported by Hainan Provincial Natural Science Foundation of China(322MS114).
文摘[Objectives]This study was conducted to clarify the biological characteristics of the pathogen Phyllosticta capitalensis,the causal agent of freckle disease on Cavendish banana in Hainan Province,China.[Methods]The impact of various nutritional and environmental factors,including media,carbon sources,nitrogen sources,temperature,pH and light on the growth and sporulation of P.capitalensis was assessed using two distinct methods:mycelium growth rate and blood counting chamber.[Results]The mycelial growth and sporulation of P.capitalensis on different media exhibited notable differences.The use of banana leaf extract dextrose agar(BLEAD)and carrot agar(CA)was observed to facilitate rapid mycelial growth.The potato dextrose agar(PDA)and potato sucrose agar(PSA)were conducive to the production of conidia.The utilization of distinct carbon and nitrogen sources exerted a pronounced influence on the growth of P.capitalensis.Maltose,dextrose,fructose,and casein acid hydrolysate were the preferred substrates for mycelial growth.The tested carbon and nitrogen sources did not significantly stimulate conidial production,whereas dextrose and NaNO 3 were found to favor sporulation.The optimal temperature for mycelial growth and conidial production was determined to be 28 and 32℃,respectively.No mycelial growth was observed at 5℃.Active mycelial growth was observed at pH 6-10,with pH 6-7 being particularly conducive to sporulation.Complete darkness was conducive to mycelial growth and sporulation.[Conclusions]It is recommended that BLEDA and PDA should be incubated at 28℃for 14 d in the dark for the purpose of mycelial growth and sporulation of P.capitalensis,respectively.
基金supported by a postgraduate scholarship from the Sarawak State Government,Malaysia.
文摘The identity of the casual agent of freckle disease of banana was investigated.The pathogen is generally referred to in literature under its teleomorphic name,Guignardia musae,or that of its purported anamorph,Phyllosticta musarum.Based on morphological and molecular data from a global set of banana specimens,several species were found associated with freckle disease.Phyllosticta maculata(from Southeast Asia and Oceania)is introduced as a new name for Guignardia musae,and an epitype is designated from Australia.Phyllosticta musarum(from India and Thailand)is shown to represent a distinct species,and the name is fixed by designation of an epitype from India.Guignardia stevensii is confirmed as distinct species from Hawaii,while Guignardia musicola from northern Thailand is shown to contain different taxa and is regarded as nomen confusum.Phyllosticta cavendishii is described as a new,widely distributed species,appearing primarily on Cavendish,but also on non-Cavendish banana cultivars.
文摘琯溪蜜柚黑斑病(pomelo black spot)的病原菌为亚洲柑橘叶点霉Phyllosticta citriasiana。田间系统观察和病原菌侵染试验结果表明,蜜柚黑斑病是一种潜伏侵染病害,上年度被侵染的带菌叶片为次年病害的侵染源,蜜柚花期至幼果期是病菌侵染盛期和病害防治适期。如果错过这两个时期,将难以达到理想的防治效果。
