Effects of Fermented Puffed Feather Meal on Growth Performance,Serum Biochemical Indices,Meat Quality,and Intestinal Microbiota in Broilers

This study aimed to investigate the effects of fermented puffed feather meal(FPFM)on growth performance,serum biochemical indices,meat quality,and intestinal microbiota in Arbor Acres(AA)broilers.A single-factor design was adopted,and four treatments were administered with five replicates to 240 one-day-old AA broilers.The control group(group A)received a basal diet,while the experimental groups received a basal diet plus 33%(group B),67%(group C)and 100%(group D)FPFM,respectively.Compared with group A,(1)the average daily gain(ADG)in group C decreased(P<0.05),and the feed conversion ratio(FCR)in group D increased(P<0.05);(2)the level of serum urea nitrogen in treatment groups decreased(P<0.05),and the levels of triglyceride,high density lipoprotein,low density lipoprotein,cholesterol,and glucose contents in group D increased(P<0.05)at day 21;(3)the serum immunoglobulin M and immunoglobulin G in group B and the immunoglobulin A in group C increased(P<0.05)at day 21,and the serum immunoglobulin M and immunoglobulin G in group D decreased(P<0.05)at day 42;(4)the share force of breast muscle and thigh muscle in group D increased(P<0.05);(5)the villus height to crypt depth ratio in the jejunum of group B increased(P<0.05)at day 21,and the villus height in group C and D increased(P<0.05)at day 42;(6)the proteobacteria counts in the cecum digesta in treatment groups decreased(P<0.05)at day 21.The basal diet supplemented with 33%FPFM promoted protein metabolism,enhanced immunity and improved meat quality,promoted the digestion and absorption of nutrients,increased intestinal microbial diversity,and improved the content of beneficial bacteria without affecting the growth performance,it was possible to be used as a good substitute for fish meal.

Effects of Remaining Leaf Combining with IBA on Rooting,Physiological and Biochemical Indicators of Leaves from Bougainvillea spectabilis Cuttings

[Objective] The research aimed to discuss the effects on rooting,physiological and biochemical indicators of Bougainvillea spectabilis cuttings by remaining leaf combining with soaking cuttings in IBA.[Method] Effects of remaining leaf combining with IBA on rooting,physiological and biochemical indicators of B.spectabilis cuttings were studied under the regulation and control of intelligent seed propagation system,and then set four treatments：remaining leaf combining with soaking in clean water,remaining leaf combining with soaking in IBA,defoliation combining soaking in clean water,and defoliation combining with soaking in IBA.[Result] The soluble sugar content was high,but indoleacetic acid oxidase activity was lower in the initial stage of adventitious root differentiation in treatment of remaining leaf.While the rooting number,root length,the root mass growth and survival rate of cutting were obviously higher than that in treatment of defoliation.Compared to the treatment soaking in clean water,the treatment soaking in 500×10^-6 IBA increased the soluble sugar content in leaves of cuttings and enhanced rooting of cuttings.[Conclusion] The survival rate and rooting of cuttings improved obviously in the treatment of remaining leaf combining with soaking in 500×10^-6 IBA before cutting under the regulation and control of intelligent seed propagation system.

Study on Effect of Drought Stress with PEG-6000 on Physiological and Biochemical Indicators in Kadsura coccinea

[Objective] The aim was to research drought resistance and adaptation mechanisms of Kaclsura coccinea under different drought stresses. [Method] The seedlings of Kadsura coccinea growing one year were taken as materials in order to investigate and analyze leaf SPAD value, relative water content （RWC）, malonaldehyde （MAD）, cell membrane permeability （RP）, proline （PRO） and superoxide dismutase activity （SOD）. [Result] As drought stress improved, leaf SPAD value and RWC were both diminishing and RWC fell remarkably; MAD, Pro, RP and SOD activity were increasing; MAD and RP grew significantly. [Conclusion] Kadsura coccinea is much sensitive to drought stress and will be affected if water loss is too much high.

The influences of SE infection on layers' production performance,egg quality and blood biochemical indicators

Background： Salmonella enterica serovar Enteritidis （SE）, as a major cause of foodborn illness, infects humans mainly through the egg. However, the symptom of laying hens usually is not typical and hard to diagnosis. In the present study, it is studied that the influences of SE infection on layers＇ performance, egg quality and blood biochemical indicators. It will help us to improve the strategy to control SE infection in commercial layers. One hundred layers at 20 wk of age were divided into 2 groups, 60 hens for experiment and others for control. The experiment group was fed with the dosage of 108 CFU SE per hen. The specific PCR was used to detect the deposition of SE. On the 8 d after SE infection, 10 hens from the control group and 30 hens from the experimenta group were slaughtered to detect the SE colonization. The production performance, egg quality and blood biochemical indices were also analyzed. Results： The results showed that the colonization rate of SE was highest in caecum contents （55.17%） and lowest in vagina （17.24%）. For the eggs the detection rate of SE was highest on the eggshell （80.00%） and lowest in yolk （18.81%）. SE infection had no significant influence on production performance and egg qualities （P 〉 0.05）. The difference of laying rate between the experimental and control groups was less than 0.30%, and both were approximately equal to 82.00%. The blood analysis showed that the aspartic aminotransferase （AST） and alanine aminotransferase （ALT） of experimental group was significantly higher than those of control group （P 〈 0.05）. For experimental and control groups AST values were 236.22 U/I and 211.84 U/I respectively, and ALT values were 32.19 U/I and 24.55 U/I. All of coefficients were less than 20%. The colonization of SE in organs increases the enzyme activities of AST and ALT in blood. Conclusions： SE in feed could invade the oviduct and infect the forming eggs. It significantly increased the concentration of ALT and AST in blood. However,SE infection was hard to be observed from the appearances of layer and egg. It might be a dangerous risk to human health.

Effects of Dietary Concentrate to Forage Ratios on Production Performance and Serum Biochemical Indicators in Post-fattening Hainan Yellow Cattle

[Objective]The paper was to investigate the effects of dietary concentrate to forage ratios on production performance and serum bio-chemical indicators of post-fattening Hainan Yellow Cattle.[Method]Sixteen Hainan Yellow Cattles with the same genetic backgrounds and similar initial weight of(242.22 ±0.71 kg) were randomly divided into four treatments.Cattles were fed with diets of different concentrate to forage ratios(20:80, group Ⅰ;30:70, Group Ⅱ;40:60, group Ⅲ;50:50, group Ⅳ).The trial lasted 62 d.[Result]The daily gain in experimental group Ⅳ was significantly higher than that in experimental group Ⅱ(P<0.05), but the feed gain ratio in experimental group Ⅳ was lower than those in other ex-perimental groups(P>0.05).The feed intake in experimental group Ⅳ was also markedly higher than those in experimental groups Ⅰ and Ⅱ(P<0.05).Compared with the experimental group Ⅱ, the gross profit of experimental group Ⅳ was significantly promoted(P<0.05).The alanine aminotransferase and aspartate aminotransferase activities in experimental group Ⅱ were markedly higher than those in experimental groups Ⅰ, Ⅲ and Ⅳ.[Conclusion]When the dietary concentrate to forage ratios was 50∶50, the production performance of post-fattening Hainan Yellow Cattle was better.

Impact of Asarum Essential Oils on Physiological and Biochemical Indicators of Two Agricultural Pests

[Objective]The paper was to study the effects of asarum essential oils on physiological and biochemical indices of two agricultural pests. [Method]Asarum essential oils were extracted by different methods,and their effects on physiological and biochemical indices related to the structure of body wall（ chitin and phenoloxidase）,the physiological and biochemical indices acting on the nervous system（ Acetylcholinesterase）,and the physiological and biochemical indices acting on the digestive system（ protease,amylase and lipase） of Ostrinia furnacalis and Mythimna seperata were studied. [Result]Asarum essential oils had no effect no chitin content of body wall under the experimental concentration,and had no inhibitory effect but activated effect on polyphenol oxidase activity. Asarum essential oils could act on the nervous system of insect,and had strong inhibitory effect against acetylcholinesterase activity of O. furnacalis larvae,with inhibition rates of 24. 86%- 38. 79%. Asarum essential oils showed a significant activated effect on pepsin activity in midgut,but the activated amplitude became smaller with the prolongation of treatment time. Asarum essential oils showed a significant inhibitory effect on activities of amylase and lipase,indicating that it could play an insecticidal effect by inhibiting activity of amylase and lipase,and it could act on digestive system of insect. [Conclusion]The study laid the foundation for further development of asarum as botanical pesticide.

The Influence of Lairage Conditions for Finishing Pigs on Behavior,Biochemical Indicators and Meat Quality

A total of 60 crossbred(Large White×Landrace) pigs of halothane genotype NN(castrated males and females) were allotted to three treatments:3 h lairage with toys,3 h lairage and 0 h lairage in a randomized complete block design and used to evaluate the influence of lairage conditions on behavior, biochemical indicators and meat quality for finishing pigs at slaughter.Behavior of the pigs was scored subjectively during lairage.Blood samples were taken at exsanguination s to measure blood temperature, plasma Cortisol,ACTH,glucose,lactate,plasma enzymes and hematological indices.Post-mortem meat quality measurements included muscle colour value(MCV),electrical conductivity(EC),pH at 45 min and 24 h from Longissimus thoracis(LM) and Semimembranosus(SM) mucles and drip loss from LM. The results showed that 3 h lairage group with toys demonstrated significantly improved behavior than the group without toys at 3 sampling times.All the pigs showed increasing calmness as the time of lairage progressed.The omission of lairage increased plasma Cortisol,ACTH,glucose and lactate(P【0.05),and decreased plasma lactate dehydrogenase(LDH),and creatine kinase(CK)(P【0.05).No biochemical index was influenced by the presence or absence of toys during lairage(P【0.05).Muscle colour value, electrical conductivity,pH at 45 min and 24 h from LM and SM and drip loss were not affected by any treatment(P【0.05).Pigs provided 3 h lairage,with or without toys,exhibited lower red blood cell(RBC), hemoglobin(HGB),and haematocrit(HCT) when compared to 0 h lairage.3 h lairage with or without toys resulted in higher white blood cell(WBC) and lymphocyte(W-SCC) levels than 0 h lairage.None of the hemocytic indices in pigs given lairage was affected by the presence or absence of toys.We conclude from this pilot study that in local commercial conditions,from the point of view of animal welfare and meat quality,lairage time of 3 h after short travel was beneficial.Pigs resting showed increased relief from stress and a recovery in immune competence.Holding pigs in lairage with toys for a few hours after arrival at the abattoir may be beneficial for the animal’s well-being.

Determination of Physiological and Biochemical Indicators of Brown-Egg Dwarf Layers at Different Ages

[ Objective] To observe biological characteristics of brown-egg dwarf layers. [ Method ] Physiological and biochemical indicators of brown-egg dwarf layers were determined at the age of 20, 40 and 60 weeks, respectively. Fifty-four chickens were detected, nine roosters and nine hens at each age. [Result] As analyzed by ttest and multiple comparisons, during 20 -60 weeks old, the RBC, HGB, MPV, PDW, LYMPH%, TP and CHO showed an increasing trend. However, during this period, the HCT, MCV, RDW, WBC and LYMPH first decreased and then de- clined, while the MCH, MCHC, PLT, PCT, MID, MID%, GRAN, GRAN%, AST, ALB, TG, ALT and GLU changed contrarily. The chickens at different ages had significantly different physiological and biochemical indicators （P 〈 0.05）. The RBC, HCT, HGB, WBC, LYMPH and AST were significantly different between the roosters and hens during 20 -60 weeks old. [ Conclusion] In the brown-egg dwarf layers, the physiological and bi- ochemical indicators vary with aging, and their difference levels also change with aging.

Biochemical indicators for neuronal regeneration during intrathecal triamcinolone application in multiple sclerosis

Multiple sclerosis （MS） is a chronic inflammatory disease of the central nervous system （CNS）. Essential charac- teristics are demyelination, inflammation and neurode- generation. This process affects the white and grey matter in the CNS. MS patients experience various progression subtypes in association with the cerebral or spinal, acute inflammatory or glial sclerotic lesions （Mtiller, 2009）. Most patients end up in a progressive, smouldering, chronic inflammatory process （MUller, 2009）. Current predominantly used 1.5 respectively 3 Tesla MRI with Gadolinium~ application visualize the various old and acute lesions. They serve as a biological marker in com- bination with standardised assessment of brain atrophy, black holes, etc. However, MRI with a stronger magnetic 7 Tesla field with better sensitivity gave hints on an on- going, acute inflammatory, smouldering process even with Gadolinium~ enhancing acute lesions in the brain and the spinal cord in progressive, so-called relapse free MS patients （Mtiller, 2009; Sinnecker et al., 2012）. Ad- ditionally, progress of MS is determined with subjective standardised clinical ratings （Sinnecker et al., 2012）. Both methods are used for the evaluation of the efficacy of relapse rate reducing drugs. These compounds, i.e., in- terferons, teriflunamide, glatiramer acetate, fingolimod, fumarate or monoclonal antibodies, preponderantly weaken the malfunction of the peripheral immune system in relapse remitting MS patients. These MS drugs share one common disadvantage. They do not stop progression or improve MS within a framework of a regenerative process. They do not enable reversal of symptoms, for in- stance functional deficits or spasticity （Mtiller, 2009）.

Detection and analysis of biochemical indicators, antioxidant enzymes and related factors in patients with Parkinson

Objective: To explore the changes and clinical significance of biochemical indexes, antioxidant enzymes and related factors in patients with Parkinson. Methods: 50 patients with Parkinson's disease who were treated in our hospital from March 2016 to March 2017 were selected as observation group. 50 healthy volunteers who were received physical examination in this hospital during the same period were selected as control group. The levels of bilirubin, blood uric acid, superoxide dismutase, glutathione peroxidase, catalase, TNF-α, tumor necrosis factor-α receptor-1, tumor necrosis factor-α receptor-2, monocyte chemoattractant protein-1 and macrophage inflammatory protein -1 of the patients in the two group were compared. Results: The level of serum bilirubin, uric acid, SOD, GSH-PX and CAT of patients in the observation group were (5.65±0.61) μmoL/L, (262.96±26.38) μmoL/L, (92.65±9.35) U/mL, (18.96±2.03) U/mL and (56.94±16.23) U/mL. The above indexes of patients in the observation group were significantly lower than those of patients in the control group (P<0.05). The serum level of TNF-α, sTNFR1, MCP-1 and MIP-1 of patients in the observation group were (19.65±2.07) pg/mL, (798.96±80.20) pg/mL, (43.65±4.27) ng/L and (23.96±2.41) ng/L. The above indexes of patients in the observation group were significantly higher than those of patients in the control group (P<0.05). Conclusion: Bilirubin, uric acid, antioxidant enzymes and inflammatory factors are all related to the progression of Parkinson's disease and can be used as auxiliary index in the diagnosis and treatment of Parkinson's disease.

Effects of Milk Replacer on the Performance and Blood Biochemical Indices in Lambs

[Objective] In this study, the performance and blood biochemical indices in lamps fed on milk replacer were researched, the feasibility of early weaned in lamps was discussed. [Method] 37 of 10 days lambs of Suffolk（♂） x XiaoWeiHanYang（ ♀ ） were randomly divided into group A, B, C, D for this experiment. Group A, B, C were fed by 3 milk-replacers containing milk for different protein source respectively, and ten Iambs in each group; group D （seven lambs） were fed by ewes as the control. The performance and blood biochemical indices of the lamps were determined. [ Result] The results showed that the average daily gains of 10 - 15 days of age was not significantly different between group A and D （ P 〉 O. 05 ）, the control were higher significandy than the group B,C （P 〈0.05）. The average daily gains of 16 -25, 26 -35 days of age were not significantly different in group A, B, C, D. The daily weight gains of Group A, B, C in 45 - 55 days of age were higher significantly than the control （P〈0.05 ）. Plasma urea nitrogen （PUN） of group B and C were higher than group A significantly （P〈0.05 ） ;Total protein （ TP）, Plasma albumin （ALB） and Plasma total amino acid were no significant difference among the other 3 groups （P〉0.05 ）. [Conclusion] So, the milk-replacers were available for early-weaned lamb.

Effect of Dietary Supplementation with Hydrolyzed Wheat Gluten on Growth Performance, Cell Immunity and Serum Biochemical Indices of Weaned Piglets (Sus scrofa)

To investigate the effects of dietary supplementation with hydrolyzed wheat gluten （HWG） on growth performance, cell immunity and serum biochemical indices of weaned piglets, 160 crossed （Large White×andrace） and weaned piglets were randomly divided into 4 treatments with 4 replicates of 10 piglets each. The piglets in each treatment were fed an experimental diet containing either 0 g kg-1 HWG （control group）, 30 g kg-1 HWG （3% HWG group）, 50 g kg-1 HWG （5% HWG group）, or 2.5 g kg-1 glycyl-L-glutamine （0.25% Gly-Gln group）. The results showed that the diarrhea rates in 3% HWG and 5% HWG groups were significantly lower than in control group from d 1 to 14 （P〈0.05）, while the average daily gain （ADG） in each of two groups was increased （P〉0.05）. When compared with control group, dietary supplementation with 3% HWG increased the ratio of CD4＋：CD8＋ and the ratio of serum albumin and globulin concentrations （A：G） on d 14 and 28, as well as the proliferation of T- and B-lymphocytes （P〉0.05） on d 28. In addition, on d 14 and 28, the A：G ratio in 5% HWG group was significantly higher than in control group （P〈0.05）, while the ratio of CD4＋：CD8＋ increased slightly （P〉0.05）. Interestingly, 0.25% Gly-Gln group had higher proportion of CD3＋ （P〉0.05） and CD4＋ （P〈0.05） on d 14 than control group, but growth performances of 0.25% Gly-Gln group were negatively affected at all experiment stages. These results suggested that HWG might improve the growth performance of piglets by strengthening cell immunity and decreasing the occurrence of diarrhea during the prophase after weaning.

Study on Growth,Serum Biochemical and Antioxidant Enzyme Indices of Juvenile Siganus guttatus Fed with Enteromorpha prolifra and Artificial Feed

In order to provide reference for using Sigartus guttatus to control overgrowth of Ertteromorpha prolifra, the growth, serum biochemical and antioxidant enzyme indices of juvenile S. guttatus respectively fed with E. prolifra and artificial feed were studied. One hundred and eighty individuals were cultivated for 90 days in six 2.5 m × 1.5 m × 1.5 m cages （30 ind. per cage） which mesh size were 0.5 mm. The experimental animals were divided into two dietary groups （ three cages for each group） that were fed with E. prolifra and artificial feed respectively. During the culture period, temperatures ranged from 23.0 to 26.5℃, pH was between 7.8 and 8.2, dissolved oxygen was more than 5.0 mg/L. The weight gain rate, specific growth rate, relative growth rate, hepato-somatic index of juvenile S. guttatus fed with E. prolifra were significantly lowe than that fed with artificial feed （P 〈 0.05）. The total protein （TP）, urea nitrogen （UN） and alkaline phosphatase （AKP） of juvenile S. guttatus fed with E. Prolifra were significantly higher than that fed with artificial feed （P 〈0.05）, while glutamic oxalacetie transaminase （AST/GOT） and glutamic-pyruvic transaminase （ALT/GPT） of juvenile S. guttatus fed with E. prolifra were significantly lower than that fed with artificial feed （P 〈 0.05 ）. SOD, GPX and anti-superoxide anion radical activity in liver, kidney, gill and muscle of juvenile S. guttatus fed with E. prolifra were higher than that fed with artificial feed, and these antioxidant enzyme activities in kidney of juvenile S. gtatatus fed with E. prolifra was significantly higher than that fed with artificial feed （P 〈 0.05）, while CAT and hydroxyl radical-inhibiting activity in liver of juvenile S. guttatus fed with arti- ficial feed were significantly higher than that fed with E. prolifra （P 〈 0.05 ）. Juvenile S. guttatus fed with E. prolifra showed poor growth performance but better antioxidant defense system. S. guttatus is easily raised and has stronger ability of digestion and absorption of E. Prolifra. The technique that uses S. gtatatus to control E. prolifra deserves deeply study.

Changes in Several Physiological and Biochemical Indices of Maize Seedling Roots Caused by Drought Stress

The changes in several physiological and biochemical indices of seedling roots of new maize variety Qingnong 8 were studied under the simulated drought condition with 18% PEG-6000 and water shortage. The results showed that under drought conditions, the contents of soluble protein and malonialdehy （MDA） and the activities of superoxide dismutase （SOD） and peroxidase （POD） in roots of maize seedlings significantly increased, and the increasing amplitude reduced after water shortage for 96 h. The re-watering treatment results after 48 h water shortage showed that the SOD and POD activities and the MDA content could recover to normal level, and the soluble protein content was lower than normal content. This study showed that the maize seedlings of Qingnong 8 suffered drought injury could grow normally after re-watering treatment.

Epidemiological features and dynamic changes in blood biochemical indices for COVID-19 patients in Hebi

BACKGROUND Millions of people have died of coronavirus disease 2019(COVID-19)due to severe acute respiratory syndrome coronavirus 2(SARS-Co V-2)infection,and retrospective studies of the disease in local regions are necessary.AIM To characterize the epidemiological features and dynamic changes in blood biochemical indices for SARS-Co V-2-infected patients in Hebi,a representative city with a large floating population in North China.METHODS From January 25 to February 10,2020,the clinical data of patients who tested positive for SARS-Co V-2 by quantitative real-time polymerase chain reaction in Hebi city(China)were evaluated at admission,and laboratory data for hematologic parameters,inflammatory indices,coagulation function indices,liver function indices,blood lipid indices,renal function indices,myocardial enzyme activities and five blood biochemical markers of immunity were evaluated at admission,upon hospitalization and before discharge.RESULTS Sixteen confirmed COVID-19 patients developed pneumonia but were cured after adequate treatment.Fever and fatigue were the common symptoms.The most common laboratory abnormalities of patients at admission were leukopenia,eosinopenia,decreased percentage of eosinophils,elevated high sensitivity C-reactive protein and fibrinogen levels,hypoalbuminemia,mildly increased aspartate transferase activity and levels of bilirubin,and increased levels ofβ2-microglobulin.Importantly,aggravated liver dysfunction was detected in most patients,which may be partially attributed to virus infection as well as medicinal treatment.CONCLUSION This study provides several potential diagnostic markers and dynamic biochemical indices of disease progression to better prevent,diagnose and treat COVID-19 infection.

Bio-Effect of Rare Earths in RE-High Background Region I. Some Blood Biochemical Indices from Population Resided in Light REE District

The results of biochemical indices in human blood show that there are significant difference in the population mean of these indices between the LREE high region in South Jiangxi, China and the control. In comparison with the normal region, the contents of total serum protein(TSP), albumin(AL), β globulin (β G), glutamic pyruvic transitanase (GPT), serium triglycerides (STG) and immunoglobulin(IgA) are manifested as low, but high cholesterol (CHO) in the LREE high region. These deviations may be related to the REE concentration and composition in food chains, and also are sex dependent. Certain blood indices (such as TSP) of different age groups in the LREE high region indicate that the influence of REE on males is a one way irreversible process while females show a strong ability of restoration.

Effects of Coated Methionine on the Growth Performance, Blood Biochemical Indices and Nutrient Apparent Ileal Digestibility in Broilers

[ Objective] This trial was conducted to study the effects of different coated methionines on the growth performance, blood biochemical indices and apparent ileal digestibility of amino acids in broilers. [ Method ] A total of 3 780 Cobb 500 broilers aged 18 days were randomly arranged into 7 groups with 3 replicates per group and 180 broilers per replicate. Control group （A） received the basal diet supplemented with O. 16% DL-me- thionine, and trial groups received the basal diets supplemented with 0.32% （ B）, 0.29% （ C）, 0.26% （ D）, 0.22% （ E）, 0.19% （F） and 0.16% （G） of coated methionine, and broilers were fed to the age of 42 days. ＂[ Result] The final weight, average daily gain and average daily feed intake of groups B and C were higher than those of the control group （ P〈0.05）, and the mortality of group B was lower than that of the control group （ P 〈0.05）, but there was no significant difference in all above indices between the other groups and the control group （P 〉 0.05）. Plasma GSH-Px activity of trial groups （ except group G） was higher than that of the control group （ P 〈 0.05）, and SOD activity of groups B and C were higher than that of group G （P 〈0.05）, but there were no significant differences in other blood biochemical indices among all groups （P 〉0.05）. There were no significant differences in apparent ileal digestibility of dry matter and crude protein between trial groups and the control group （ P 〉0.05 ）, and the apparent ileal digestibility of amino acids of trial groups was increased （P 〈0.05） or no change expect that the apparent ileal digestibility of many kinds of amino acids of group G was decreased compared with the control group （P〈0.05）. [ Concluslon~ The growth performance and apparent ileal digestibility of amino acids in broilers were increased with the rising supplementation of coated methionine, but the supplementation of 0.19% coated methionine can meet the needs of broilers, namely, coated methionine can be substituted for 60% DL-methionine in the diet, so DL-methio- nine can be saved up to 40%.

Effects of Additives of Traditional Chinese Medicine on Blood Biochemical Indices of Wenchang Chickens in Hainan

[Objective]The aim was to study on effects of additives of traditional Chinese medicines on biochemical indices of blood of Wenchang chickens in Hainan. [Method]About 180 female Wenchang chickens were divided into five groups at random with three repetitions for every group and twelve chickens for a repetition. Group 1 was control group and the chickens were fed with basic forages; the 2nd group was control group of antibiotics and the chickens were fed with basic forages and 600 mg /kg of aureomycin; group 3,4 and 5 contained 0. 1%,0. 2% and 0. 3% of the additives respectively,besides basic forages. Chickens at average weight were chosen six from every group on 35th and 56th days,respectively, to prepare serums for biochemical indices of bloods and analyze effects of additives on the indices. [Result]Serum cholinesterase activity and albu- min content were both improved by 0. 1%,0. 2% and 0. 3% of the additives （ P 〈0. 05） ; activity of alkaline phosphatase was enhanced,and serum cholesterol was lowered both by 0. 2% and 0. 3% of the additives （ P 〈0. 05） . [Conclusion]Additives of traditional Chinese medicines have an ac- tive effect on blood biochemical indices of Wenchang chickens.

Correlation analysis of TCM syndrome types with T lymphocytes and biochemical indices in patients with HBV-related primary liver cancer

Objective:To investigate the correlation between T lymphocytes and biochemical indices in patients with Primary liver cancer(PLC)associated with hepatitis B virus(HBV)and TCM syndrome differentiation.Methods:263 HBV-related PLC patients who were admitted to the First Affiliated Hospital of Henan University of Traditional Chinese Medicine from January 2018 to December 2019 were retrospectively collected.There were 127 cases of liver depression and spleen deficiency syndrome(48.3%),48 cases of spleen deficiency and dampness syndrome(18.3%),31 cases of liver and gallbladder dampness and heat syndrome(11.8%),35 cases of liver and blood stasis syndrome(13.3%),and 22 cases of liver and kidney Yin deficiency syndrome(8.4%).The general data,T cell subsets,oncology and virology indicators,oncology characteristics,biochemical indicators and other data were counted.Epidata and Excel were used to collect and summarize the data,and SPSS26.0 software was used for statistical analysis.Results:There was no significant difference in gender and age distribution among the five syndrome types(χ^(2)=5.462,F=1.979,ALL P>0.05).The differences among T lymphocyte count(χ^(2)=57.785,P<0.001),CD4(+)T cell count(χ^(2)=47.103,P<0.001)and CD8(+)T lymphocyte count(F=12.760,P<0.001)were statistically significant.The T lymphocyte count,CD4(+)T lymphocyte count and CD8(+)T lymphocyte explicit count in patients with liver and kidney Yin deficiency syndrome were significantly lower than those in the other four syndrome types.AFP(χ^(2)=89.986,P<0.001),CEA(χ^(2)=95.501,P<0.001),CA199(χ^(2)=30.044,P<0.001)of the five syndrome types increased successively from the syndrome of liver depression and spleen deficiency to the syndrome of liver and kidney Yin deficiency,and the difference was statistically significant.There were statistically significant differences in the inner diameter of main portal vein,portal vein cancer thrombin and extrahepatic metastasis among the five syndrome types(ALL P<0.001).The main symptoms of portal vein cancer thrombin and extrahepatic metastasis were liver-gallbladder dampness-heat syndrome and liver-blood stasis syndrome.The differences among PLT(χ^(2)=39.234,P<0.001),Alb(χ^(2)=75.171,P<0.001),TBil(χ^(2)=51.140,P<0.001),AST(χ^(2)=55.881,P<0.001),PT(χ^(2)=21.515,P<0.001)were statistically significant.PLT and Alb decreased successively from the syndrome of liver depression and spleen deficiency to the syndrome of liver and kidney Yin deficiency.PLT and Alb of the syndrome of liver depression and spleen deficiency were significantly higher than those of the other four groups,and TBil and AST of the syndrome of liver and gallbladder dampness and heat were significantly higher than those of the other four groups.PT of liver and kidney Yin deficiency was significantly higher than that of the other four groups.The lymphocyte count,CD4(+) lymphocyte count and CD8(+) lymphocyte count were negatively correlated with AFP,PT and TBil(ALL P<0.05),and positively correlated with PLT(P<0.05).T lymphocyte count was positively correlated with AIb(P<0.05).Conclusion:This study found that patients with liver depression and spleen deficiency syndrome have better cellular immune function,liver function and prognosis.Patients with liver and kidney Yin deficiency have lower cellular immunity,worse liver function,and worse prognosis.Portal vein carcinoma embolus and extrahepatic metastasis were mainly characterized by dampness and heat of liver and gallbladder and blood stasis of liver.Patients with lower lymphocyte counts have poorer blood clotting,worse the liver reserve,and the higher the risk of further cancer.

Determination of Physiological and Biochemical Indices of Hailan Chickens at 20 Weeks of Age

[Objective]This study aimed to determine physiological and biochemical indices of Hailan chickens at 20 weeks of age.[Method]T,P,BT,CT,ESR,systolic pressure,diastolic pressure,RBC,HCT,MCV,RDW,HGB,MCH,MCHC,PLT,MPV,WBC,N,E,B,L,M,TP,Alb,AST,ALT,Glu and TG and CHO of 20 weeks-old male and female Hailan chickens were determined with various methods.[Result]According to the determined blood physiological and biochemical indices,RBC,HGB,Glu and ALB in male chickens were higher compared with female chickens,exhibiting extremely significant differences（P〈0.01）;B,M and TP in male chickens were significantly higher compared with female chickens（P〈0.05）;other physiological and biochemical indices exhibited no significant differences between males and females（P〉0.05）.[Conclusion]Physiological and biochemical indices of 20 weeks-old Hailan chickens varied between males and females,which were generally higher in male chickens than in female chickens.