Preliminary study on seasonal succession and development pathway of phytoplankton community in the Bohai Sea

Phytoplankton species composition and species succession were determined in 1998-1999 based on 2 nestle investigation cruises in the Bohai Sea and two monthly monitoring stations at Penglai and Changdao for 15 months. The seasonal succession and pathway of phytoplankton community in the Bohai Sea were discussed complementarily with history data. The main process of Phytoplankton community development in the Bohai Sea was controlled by temperature and nutrient replenishes. There were two cell abundance peaks in an annual variation, the main peak in April and the secondary peak in September. In winter, the cell abundance was low due to the low temperature, the phytoplankton community was mainly made up of small-celled diataoms. In spring, the phytoplankton community was developed very quickly by small-celled diatom in suitable conditions of temperature and nutrients. In summer, the cell abundance decreased and big-celled diatoms became predominated. In autumn, because of the replenish of nutrient, big-celled diatoms and dinoflagellates formed another cell abundance peak. during the annual variation of phytoplankton community in the Bohai Sea, species succession was the main process of community development, the species sequence just occur at special areas and special periods. The evolution of phytoplankton community in the Bohai Sea accords with the hypothesis of Margalef's phytoplankton community of four stages. But the size feature is contrary to the hypothesis, which may be caused by nutrient replenish in autumn in Bohai Sea and the top to down control.

Present status and changes of the phytoplankton community after invasion of Neosalanx taihuensis since 1982 in a deep oligotrophic plateau lake, Lake Fuxian in the subtropical China

Phytoplankton assemblages in the subtrophical oligotrophic Lake Fuxian, the second deepest lake in China, were investigated monthly from September 2002 to August 2003. A total of 113 species belonging to seven phyla were identified, among them, a filamentous green alga, Mougeotia sp., dominated almost throughout the study period and comprised most of the total phytoplankton biomass. Mougeotia sp. has made a substantial development during the past decades: it was absent in 1957, only occasionally present in 1983, increased substantially in 1993, and became predominant in 2002—2003. It is likely that natural invasion of the Taihu Lake noodlefish(Neosalanx taihuensis) has led to a change of dominant herbivorous zooplankton from small to large calanoid, which has increased grazing pressure on small edible algae, and thus has indirectly favored the development of the inedible filamentous Mougeotia sp.

Phytoplankton community structure in reservoirs of different trophic status, Northeast China

The aim of this study was to determine the phytoplankton community structures of reservoirs of different trophic status, located in a cold region. Physical and chemical variables and the phytoplankton communities were investigated in two reservoirs (Xiquanyan Reservoir and Taoshan Reservoir) in Northeast China in 2009. The two reservoirs showed strong seasonal fluctuations in their physical and chemical composition. Results of the trophic status index indicated that Xiaquanyan Reservoir was mesotrophic, whilst Taoshan Reservoir was eutrophic. Diatoms were the dominant phytoplankton group in Xiquanyan Reservoir throughout all seasons of the study, while in Taoshan Reservoir, diatoms dominated in spring, and cyanobacteria dominated in summer and autumn. This difference was resulted from differences in local environmental factors, including nutrients and hydrology. This study suggests that in mesotrophic reservoirs, nutrients played a key role in controlling seasonal phytoplankton successions, whereas in eutrophic reservoirs water temperature was the key factor in a cold region. Notably, the dominant species in summer in the Taoshan Reservoir was Microcystis, which may produce toxins depending on the ambient conditions, and presenting a risk of local toxin contamination.

Long-term ecological interactions between nutrient and phytoplankton community in the Changjiang estuary

Both nitrate((NO_3)^-) and soluble reactive phosphate((PO_4)^(3-)) concentration in the freshwater end-member at the mouth of the Changjiang River have increased dramatically since the 1960s.Within the same period in the sea area,with surface salinity>30,(NO_3)^-concentration has shown an obvious increase,(PO_4)^(3-) has not changed greatly and dissolved reactive silica((SiO_3)^(2-)) has deceased dramatically.An examination of the elemental ratio of(NO_3)^-to(PO_4)^(3-) at the mouth of the Changjiang River did not show a systematic trend from the 1960s to 2000s largely because both nutrients increased simultaneously.In comparison,the elemental ratio of dissolved inorganic nitrogen(DIN) to(PO_4)^(3-) in surface seawater,with salinity>22,has shown a clearly increasing trend.Furthermore,an overall historical change of the(SiO_3)^(2-):(PO_4)^(3-) ratio has undergone a reverse trend in this area.Based on the changes of(SiO_3)^(2-):(PO_4)^(3-) and DIN:(PO_4)^(3-) ratios,we can conclude that an overall historical change of(SiO_3)^(2-):DIN ratio has decreased in this area from the 1950-1960s to 2000s.The argument that phytoplankton productivity in the Changjiang estuary has been enhanced by increasing nutrient input from the riverine transport was supported by these results.A comparative study analyzing the shift of phytoplankton composition from the mid-1980s to 2000s was also made.The results indicated that the average yearly percentage of diatom species in the Changjiang estuary has decreased from 84.6% during 1985-1986 to 69.8% during 2004-2005.Furthermore,the average yearly percentage of diatom abundance in the Changjiang estuary decreased from 99.5% during to 75.5% over the same time period,while the abundance of dinoflagellates has increased dramatically,from 0.7% to 25.4%.

Maixi River estuary to the Baihua Reservoir in the Maotiao River catchment:phytoplankton community and environmental factors

Phytoplankton and environmental variables were measured monthly from July 2009 to August 2011 in the Maixi River from the estuary to Baihua Reservoir in the Maotiao River catchment, southwestern China, to understand phytoplankton community structure and environmental factors. The relationship between phytoplankton community structure and environmental factors including hydrological, meteorological, physical, and chemical variables were explored using multivariate analysis. A total of 81 taxa of phytoplankton were identified, which were mainly composed of chlorophyta, bacillariophyta, and cyanobacteria. The phytoplankton community was dominated by Pseudanabaena limnetica during summer and fall and by Cyclotella meneghiniana during winter and spring. The abundance of phytoplankton ranged from 0.24~ 104 cells/L to 33.45x 106 cells/L, with the minimum occurring during February 2010 and the maximum during July 2009. The phytoplankton community was dominated mainly by cyanobacteria from April to September, and by bacillariophyta and pyrrophyta from October to March. Canonical correspondence analysis showed that temperature, pH values, and orthophosphate were the most important driving factors regulating the composition and dynamics of the phytoplankton community in the estuary. Cyanobacteria and euglenophyta abundance and biomass were affected mainly by temperature and pH values, while most chlorophyta and bacillariophyta were influenced by the concentrations of nutrients.

Response of phytoplankton community to different water types in the western Arctic Ocean surface water based on pigment analysis in summer 2008

Nutrients and photosynthesis pigments were investigated in the western Arctic Ocean during the 3rd Chinese Arctic Research Expedition Cruise in summer 2008. The study area was divided into five provinces using the K- means clustering method based on the physical and chemical characteristics of the sea water, and to discuss the distribution of the phytoplankton community structure in these provinces. CHEMTAX software was performed using HPLC pigments to estimate the contributions of eight algal classes to the total chlorophyll a （TChl a）. The results showed that on the Chukchi Shelf, the Pacific Ocean inflow mainly controlled the Chl a biomass and phytoplankton communities by nutrient concentrations. The high nutrient Anadyr Water and Bering Shelf Water （AnW and BSW） controlled region have high Chl a levels and the diatom dominated community structure. In contrast, in the region occupied by low-nutrient like Alaska Coastal Water （ACW）, the Chl a biomass was low, with pico- and nano-phytoplankton as dominated species, such as prasinophytes, chrysophytes and cryptophytes. However, over the off-shelf, the ice cover condition which would affect the physical and nutrient concentrations of the water masses, in consequence had a greater impact on the phytoplankton community structure. Diatom dominated in ice cover region and its contribution to Chl a biomass was up to 75%. In the region dose to the Mendeleev Abyssal Plain （MAP）, controlled by sea-ice melt water with relatively high salinity （MW-HS）, higher nutrient and Chl a concentrations were found and the phytoplankton was dominated by pico- and nano-algae, while the diatom abundance reduced to 33%. In the southern Canada Basin, an ice-free basin （IfB） with the lowest nutrient concentrations and most freshened surface water, low Chl a biomass was a consequence of low nutrients. The ice retreating and a prolonged period of open ocean may not be beneficial to the carbon export efficiency due to reducing the Chl a biomass or intriguing smaller size algae growth.

Seasonal Variability of Phytoplankton Community Structure in Relation to Different Nitrogen-Phosphorus Ratios in the Southern Coastal Waters of Zhejiang, China

With the rapid development of economy and increase of population in the drainage areas, the nutrient loading has increased dramatically in the Changjiang estuary and adjacent coastal waters. To properly assess the impact of nutrient enrichment on phytoplankton community, seasonal microcosm experiments were conducted during August 2010-July 2011 in the coastal waters of Zhejiang Province. The results of the present study indicated that the chl a concentration, cell abundance, diversity indices, species composition and community succession of the phytoplankton varied significantly with different N/P ratios and seasons. Higher growth was observed in the 64：1 （spring）, 32：1 （summer）, 16：1 （autumn） and 128：1,256：1 （winter） treatments, respectively. The values of Shannon-Wiener index （H） and Pielou evenness index （J） were lower in the 8：1 and 16：1 treatments in autumn test, while H value was higher in the 128：1 and 8：1 treatments in winter test. A definite community succession order from diatoms to dinoflagel lares was observed in the autumn and winter tests, while the diatoms dominated the community throughout the culture in the spring and summer tests.

Study of phytoplankton community in the channel from Dongting Lake to Changjiang River, China

Phytoplankton cell density, biomass, chl-α concentration and their seasonal fluctuations at five stations in the channel between Dongting Lake and the Changjiang （Yangtze） River were studied from May 1995 to December 1997. The seasonal fluctuations had two peaks in a year in general. The annual patterns of total cell density and biomass indicated that diatoms contributed more than 70 percent of the total biomass （except from June to October） during the investigation period. Twenty-one dominant species contributed about 60--80 percent of the biomass in various months. The cell density, biomass and seasonal fluctuation were similar at Stations 1-4, but at Station 5 connected directly with the Changjiang River, the annual mean values （127.34×10^4cells/L and 0.46 mg/L in 1996, 41.98×10^4ceUs/L and 0.2 mg/L in 1997 respectively） were lower than those （ 195.92× 10^4 cells/L and 0.5 mg/L in 1996, 132.96 ×10^4 cells/L and 0.57 mg/L in 1997 respectively） at Station 1-4; and the curve of the seasonal fluctuation sometimes did not coincide with those at Stations 1-4. Based on criteria for evaluating trophic status, the dominant species, annual average cell density values of 114.86× 10^4-179.57× 10^4 cells/L, biomass of 0.48-0.5 mg/L, chl-αconcentration of 0.42-0.51 μg/L respectively recorded at five stations in 1996 and 1997, and values associated with the physico-chemical properties of the waterbody indicated that the water in the channel could be characterized as oligo-mesotrophic.

Tidal water exchanges can shape the phytoplankton community structure and reduce the risk of harmful cyanobacterial blooms in a semi-closed lake

For estuaries,inland lakes play a vital role in the ecological balance under the impact of tide s.The effect of tides-induced water exchange on phytoplankton community in a semi-closed lake was studied and compared with that of an adjacent closed lake in the Oujiang River mouth in Zhejiang,East China Sea,from June 29,2020 to June 14,2021.Results show that the dominant species,abundance,dominance,and diversity of the phytoplankton species between the two lakes were significantly different.In the closed lake,cyanobacteria were the dominant species during the study period.However.in the semi-closed lake,the diversification of the dominant species was greater,and some species of diatoms and green algae became dominant.The average phytoplankton abundance in the closed lake was 6 times of that in the semi-closed lake.The average dominance of cyanobacteria in the closed lake was 0.96,and those in the semi-closed lake and the Oujiang River were 0.51 and 0.22,respectively.Cyanobacterial blooms occurred throughout the study time in the closed lake but not in the semi-closed one.Furthermore,the species diversity richness of the phytoplankton in the semi-closed lake was higher than that of the closed one,and the phytoplankton community between the closed lake and semi-closed lake could be divided into distinctly different groups based on non-metic multidimensional scaling analysis(NMDS)and analysis of similarities(ANOSIM)analysis.The salinity of the water was significantly greater and the transparency significantly smaller in the semi-closed lake than those in the closed lake.Therefore,water exchange driven by local tidal movement increased salinity and decreased transparency of water,which consequently shaped the community structures of different phytoplankton and reduced the risk of a cyanobacterial bloom outbreak in the semi-closed lake.

Ecological characteristics of phytoplankton community from eutrophic waters in the Maluan Bay

present study is based on the analysis of the data collected in 10 dnas during the periods of 2 surveys (from May 1992 to January 1993 and from August 1993 to May 1994). The results show that the number ofphytoplankton in highly eutrophic waters of the Maluan Bay is low, Only 6-35 spotes from each cruise and 66species in total fain the 2 surveys. The phytoplanha abundanCe is high. The mean cell densities of phytoplanktonfrom the net samples were 21 386x 104 cells/m3 for the first sUrVey and 110 136 x 104 cells/m3 for the second survey,that fm the water Samples was 1 150. 7 cells/cm3. The mean concentration of chlorOPhyll a reachs 6. 67 mg/m3.Both the species diversity (H') and evennes (J) were low, only 0. 78 and 0. 28, restwhvdy. The temporal and spatial fluctuation of the cell denSity of phytoplankton was quite wificant, caused banly by contribution of pennate diatom Nitzschia delicatissima that often abudanily occurred in a ofed waters rich in organic matter and could be takenas an ecologic indicator of highly eutrophic harbour. The axistence of good correlation between the cell density ofNitzschia delicatissima and some environmental variables (e. g. Po43 - P and COD, etc. ), and the heterotrophic tendancy of this species are discussed, too.

Physical structure and phytoplankton community off the eastern Hainan coast during summer 2015

Based on satellite remote sensing dataset and survey data during upwelling season of 2015,the spatial structures of phytoplankton biomass and community for the first time in the eastern Hainan upwelling(EHU)and its adjacent area,the eastern Leizhou Peninsula upwelling(ELPU)were illustrated.It is found that a significant cold tongue with high salinity and low temperature along the eastern Hainan coast driven by upwelling-favorable summer monsoon.The ELPU was relative weaker than the EHU because of its wide and gentle continental slope.Due to mixing by tides and waves,DO concentration with high value(>6.0 mg/L)were almost homogenous from surface to 30 m depth at the EHU.Beneath that,low DO water(<6.0 mg/L,anoxia)were pumped upward from bottom by the upwelling.The ELPU has worse DO condition compared with the EHU where bottom DO were lower than 3.5 mg/L owing to abundant DO consumption.The phytoplankton biomass reached maximal value about 1.5 mg/m3 at 30 m depth layer rather than surface layer at the EHU indicating the impact limit of upwelling on phytoplankton growth and DO distribution.Nourished by rich nutrient input,the phytoplankton biomass at the ELPU were much higher than the EHU where the maximal value can reach about 4.0 mg/m3.The phytoplankton biomass were reduced to about 0.2–0.3 mg/m3 at the offshore areas of the EHU and ELPU which were close to the value at open sea.At the inshore of the EHU,the phytoplankton community was dominated by diatom which accounted for about 50%of phytoplankton biomass.And prokaryotes(about 40%),green algae(about 20%)and prochlorococcus(about 20%)became main species at the offshore of the EHU.At the ELPU,diatom accounted for about 80%of phytoplankton biomass followed by green algae,indicating a different ecosystem at this region compared with the EHU.

Ecological thresholds of phytoplankton community across environmental gradients in the harmful algal blooms-frequently-occurring,subtropical coastal waters,East China Sea

Phytoplankton communities can response immediately and directly to environmental changes,and thus have been applied as reliable biotic indicators in aquatic systems.This study provided insights into the relationships concerning ecological thresholds of phytoplankton communities and individual taxon in response to environmental changes in coastal waters of northern Zhejiang Province,East China Sea.Results demonstrated that there existed seasonal variations of phytoplankton community ecological thresholds of which spring being higher than those in summer.As for individual species,Prorocentrum donghaiense and Noctiluca scintillans were identified as the most tolerant and sensitive indicator species in spring and summer,respectively.They exhibited strong indications in response to environmental changes.These findings highlighted that phytoplankton community structure in this region was stable when environmental gradients were below the thresholds of sensitive species,whereas potential harmful algal blooms may occur when environmental gradients exceeded the thresholds of tolerant species.

Phytoplankton community composition in the surrounding waters of Changli, Hebei, China

The seasonal and spatial variations of phytoplankton abundance weresupported with microscopic analyses in the surrounding waters of Changli in the spring(May) and summer (August) of 2015. Nineteen sampling stations were selected in thisstudied area (39°26′30"-39°39′ N, 119°17′30"-119°34′03"). During the survey period, atotal of 27 species of phytoplankton have been documented in spring and 63 species insummer. In terms of the community composition at the seasonal level and the spatialdistribution, Dinoflagellates was absolutely dominant in spring and Bacillariophyta wasdominant in summer. The average phytoplankton cell density of phytoplankton speciesshowed substantial seasonal differences. In addition, the dominant species in differentseasons were different. The average values of Shannon-Wiener diversity index (H')were 0.99 in spring and 3.25 in summer, which implied that species diversity in summeris higher than that in spring. Redundancy analysis (RDA) revealed that ammonium(NH4+-N) and nitrites nitrate nitrogens (NO3--N) were the important environmentalfactors influencing the phytoplankton distribution. N is the main environmental drivingfactor result from environmental indicators and RDA analysis.

Seasonal Variations in Phytoplankton Community Structure in the Sanggou,Ailian,and Lidao Bays

The seasonal variations in phytoplankton community structure were investigated for the Sanggou Bay （SGB） and the adjacent Ailian Bay （ALB） and Lidao Bay （LDB） in Shandong Peninsula,eastern China.The species composition and cell abundance of phytoplankton in the bay waters in spring （April 2011）,summer （August 2011）,autumn （October 2011）,and winter （January 2012） were examined using the Uterm6hl method.A total of 80 taxa of phytoplankton that belong to 39 genera of 3 phyla were identified.These included 64 species of 30 genera in the Phylum Bacillariophyta,13 species of 8 genera in the Phylum Dinophyta,and 3 species of 1 genus in the Phylum Chrysophyta.During the four seasons,the number of phytoplankton species （43） was the highest in spring,followed by summer and autumn （40）,and the lowest number ofphytoplankton species （35） was found in winter.Diatoms,especially Paralia sulcata （Ehrenberg） Cleve and Coscinodiscus oculus-iridis Ehrenberg,were predominant in the phytoplankton community throughout the study period,whereas the dominance of dinoflagellate appeared in summer only.The maximum cell abundance of phytoplankton was detected in summer （average 8.08 × 103 cells L-1） whereas their minimum abundance was found in autumn （average 2.60 x 103 cellsL-1）.The phytoplankton abundance was generally higher in the outer bay than in the inner bay in spring and autumn.In summer,the phytoplankton cells were mainly concentrated in the south of inner SGB,with peak abundance observed along the western coast.In winter,the distribution of phytoplankton cells showed 3 patches,with peak abundance along the western coast as well.On seasonal average,the Shannon-Wiener diversity indices of phytoplankton community ranged from 1.17 to 1.78 （autumn 〉 summer 〉 spring 〉 winter）,and the Pielou＇s evenness indices of phytoplankton ranged from 0.45 to 0.65 （autumn 〉 spring 〉 summer〉 winter）.According to the results of canonical correspondence analysis,phosphate level was the major factor that limited the occurrence of P.sulcata and C.oculus-iridis,whereas optimal temperature and low salinity were responsible for Prorocentrum blooms in summer.The detailed description of seasonal variations in phytoplankton community structure in the three bays provide reference data for future studies on marine ecosystems and mariculture in adjacent areas.

Upwelling and anthropogenic forcing on phytoplankton productivity and community structure changes in the Zhejiang coastal area over the last 100 years

Phytoplankton productivity and community structure in marginal seas have been altered significantly during the past three decades, but it is still a challenge to distinguish the forcing mechanisms between climate change and anthropogenic activities. High time-resolution biomarker records of two 210Pb-dated sediment cores（#34： 28.5°N, 122.272°E; CJ12-1269： 28.861 9°N, 122.515 3°E） from the Min-Zhe coastal mud area were compared to reveal changes of phytoplankton productivity and community structure over the past 100 years. Phytoplankton productivity started to increase gradually from the 1970 s and increased rapidly after the late 1990 s at Site #34; and it started to increase gradually from the middle 1960 s and increased rapidly after the late 1980 s at Site CJ12-1269. Productivity of Core CJ12-1269 was higher than that of Core #34. Phytoplankton community structure variations displayed opposite patterns in the two cores. The decreasing D/B（dinosterol/brassicasterol） ratio of Core #34 since the 1960 s revealed increased diatom contribution to total productivity. In contrast, the increasing D/B ratio of Core CJ12-1269 since the 1950 s indicated increased dinoflagellate contribution to total productivity. Both the productivity increase and the increased dinoflagellate contribution in Core CJ12-1269 since the 1950–1960s were mainly caused by anthropogenic activities, as the location was closer to the Changjiang River Estuary with higher nutrient concentration and decreasing Si/N ratios. However, increased diatom contribution in Core #34 is proposed to be caused by increased coastal upwelling, with higher nutrient concentration and higher Si/N ratios.

Nano-and microphytoplankton community characteristics in brown tide bloom-prone waters of the Qinhuangdao coast,Bohai Sea, China

Since 2009, the newly recorded species Aureococcus anophagefferens has successively induced brown tides in the Qinhuangdao coastal waters of the Bohai Sea. Here, we focus on the phytoplankton community structure in this region and try to point out the biological background characteristics of the outbreaks of successive brown tides. We analyzed species composition,cell abundance, diversity indices and size-fractionated chlorophyll a(Chl-a) concentration monthly from March 2013 to January2014. The phytoplankton community, as observed by microscopy, underwent succession from nano-celled chained diatoms to dinoflagellates in the spring and summer, and then to micro-celled diatoms and nano-celled chained diatoms as the most prominent groups in the subsequent autumn and winter. Canonical correspondence analysis revealed that changes in the abundance of diatoms were related to nutrient availability, especially of nitrogen, while water temperature was the crucial factor influencing the abundance of dinoflagellates and A. anophagefferens. Considering the contribution of nano-celled chained species to the micro-sized Chl-a,small phytoplankton(<20 μm) composed the majority of total Chl-a and were generally abundant during our study. Abundant and diverse small phytoplankton seemed to serve as a background or seedbank for the formation of blooms of A. anophagefferens and other small-celled algae.

Community structure and spatial-temporal variation of netzphytoplankton in the Bering Sea in summer

Marine biodiversity is changing in response to altered physical environment, subsequent ecological changes as well as anthropogenic disturbances. In this study, phytoplankton samples in situ collected in the Bering Sea in July of 1999 and 2010 were analyzed to obtain phytoplankton community structure and spatial-temporal variation between the beginning and end of this decade, and the correlation of phytoplankton community dynamics and environmental factors was investigated. A total of 5 divisions, 58 genera and 153 species of phytoplankton belonging to 3 ecological groups were identified. The vast majority of phytoplankton consisted of diatoms accounting for 66.7% of the total species and 95.2% of the total abundance. Considering differentiation in spatial extent and phytoplankton sample types, there were subtle changes in species composition, large altering in abundance and significant variation in spatial distribution between two surveys. The abundance peak area was located at the Bering Strait while sub peak was found at the Bering Sea Basin. The boreal-temperate diatom was the dominant flora, which was subsequently replaced by eurythermal and frigid-water diatom. Phytoplankton community in the Bering Sea was not a simplex uniform community but composed of deep-ocean assemblage and neritic assemblage. The deep-ocean assemblage was located in the northwestern Pacific Ocean and Bering Sea Basin, dominated by boreal-temperate species（Neodenticula seminae, Thalassiothrix longissima, Amphiprora hyperborean, Chaetoceros atlanticus, Thalassiosira trifulta, etc.） and eurychoric species（Thalassionema nitzschioides, Ch. compressus, Rhizosolenia styliformis, etc.）, and characterized by low abundance, even interspecies abundance allocations, diverse dominant species and high species diversity. The neritic assemblage was distributed on the continental shelf and slope of Bering Sea and was mainly composed of frigid-water species（Th.nordenski？ldii, Ch. furcellatus, Ch. socialis, Bacteriosira fragilis, etc.） and eurythermal and euryhaline species（L.danicus, Ch. curvisetus, Coscinodiscus curvatulus, etc.）, and it was characterized by high abundance, uneven interspecies allocations, prominent dominant species and low species diversity. Spatial-temporal variation of species composition and abundance of phytoplankton in the Bering Sea was directly controlled by surface circulation,nutrient supply and ice edge.

Phytoplanktonic biogeography in the subtropical coastal waters,East China Sea along intensive anthropogenic stresses: roles of environmental versus spatial factors

Understanding the relative roles of local environmental effects and spatial effects on phytoplankton community is of essential importance to study the biogeography of them at regional scale. However, the determinants that driving the biogeography of phytoplankton communities in the coastal area of northern Zhejiang still remained unclear. We surveyed phytoplankton community compositions in water columns associated with environmental and spatial influences across five subzones that geographically covering this region over four seasons. Diatoms and dinoflagellates were recorded as the main dominant groups and Coscinodiscus oculs-iridis, Coscinodiscus jonesianus, and Skeletonema costatum, were identified as the major abundant species existing in all seasons.Spatially structured environmental conditions, rather than pure spatial or environmental factors, substantially shaped the biogeography of phytoplankton community, with the former mainly comprised of water temperature,dissolved oxygen, phosphate, pH, and salinity, and the latter referring to a non-negligible factor. This study was the first integrated research that combining environmental filtering with spatial factors in structuring phytoplankton communities at a complete tempo-spatial scale. Our results may facilitate to the further study of harmful algal blooms early-warning in this region.

Hydrochemical characteristics and the ecological effect of algal carbonic anhydrase in carbon cycle in the Taiyuan section of Fenhe River

Water scarcity and pollution pose a threat to the sustainable development of cities and society.Therefore,it is crucial to analyze the hydrochemical characteristics and carbon dynamics of waterdeficient areas.Taking the Taiyuan section of Fenhe River as the research object,we systematically explored the hydrochemical characteristics of surface water and its evolutionary processes,as well as the ecological effect of algal carbonic anhydrase in carbon cycle using the hydrochemical evolution method and correlation analysis.The ternary diagram demonstrates that the main water chemical type in Fenhe River was SO^(2-)_(4)·Cl^(-)-Na^(+).The Gibbs and end-member diagrams of each ion display that the chemical composition of surface water was mainly controlled by silicate decomposition.The chemical ions originated mainly from dissolution of some minerals,such as plagioclase,halite,dolomite,calcite,and gypsum.The diatoms had a lower CO_(2)requirement because they exhibited a higher abundance at a lower partial pressure of CO_(2)(p CO_(2)).However,high CO_(2)concentration had a positive effect on cyanobacteria,which reduced the active transport of HCO_(3)^(-),saved the energy needed for this part of active transport,and indirectly improved the overall photosynthetic efficiency of algae.Carbonic anhydrase(CA)activity was significantly negatively correlated with p CO_(2)and positively correlated with HCO_(3)^(-)concentration,indicating that CA in water promoted the conversion of CO_(2)to HCO_(3)^(-).The HCO_(3)^(-)generated from this process continued to participate in the erosion of silicate rocks,sequestering CO_(2)in the form of Ca CO_(3),which has a non-negligible impact on the carbon sink in the Fenhe River.These consequences may have important implications for the biogeochemical cycling occurring in urban water.

Phytoplankton composition and its ecological effect in subsurface cold pool of the northern Bering Sea in summer as revealed by HPLC derived pigment signatures

CHEMTAX analysis of high-performance liquid chromatography （HPLC） pigment was conducted to study phytoplankton community structure in the northern Bering Sea shelf, where a seasonal subsurface cold pool emerges. The results showed that fucoxanthin （Fuco） and chlorophyll a （Chl a） were the most abundant diagnostic pigments, with the integrated water column values ranging from 141 to 2160 μg/m2 and 477 to 5 535 μg/m2, respectively. Moreover, a diatom bloom was identified at Sta. BB06 with the standing stock of Fuco up to 9214 μg/m3. The results of CHEMTAX suggested that the phytoplankton community in the northern Bering Sea shelf was dominated by diatoms and chrysophytes with an average relative contribu- tion to Chl a of 80% and 12%, respectively, followed by chlorophytes, dinoflagellates, and cryptophytes. Dia- toms were the absolutely dominant algae in the subsurface cold pool with a relative contribution exceeding 90%, while the contribution of chrysophytes was generally higher in oligotrophic upper water. Additionally, the presence of a cold pool would tend to favor accumulation of diatom biomass and a bloom that occurred beneath the halocline would be beneficial to organic matter sinks, which suggests that a large part of the phytoplankton biomass would settle to the seabed and support a rich benthic biomass.