采用聚丙烯酰胺凝胶电泳(PAGE)和蔗糖密度梯度超速离心方法分离了假根羽藻(Bryopsis corticulans)的色素-蛋白复合物,并对其特性进行分析。结果表明:采用PAGE分离得到7条色素-蛋白复合物带,分别是CP I a1、CP I a2、CP I、LHCP1、LHCP2...采用聚丙烯酰胺凝胶电泳(PAGE)和蔗糖密度梯度超速离心方法分离了假根羽藻(Bryopsis corticulans)的色素-蛋白复合物,并对其特性进行分析。结果表明:采用PAGE分离得到7条色素-蛋白复合物带,分别是CP I a1、CP I a2、CP I、LHCP1、LHCP2、CPa、LHCP3+3,和2条游离色素(free pigment,FP)FCa、FC。用改进的不连续蔗糖密度梯度离心法分离到五条带。区带Ⅰ是FP;区带Ⅱ主要是小分子量的PSⅡ捕光复合物LHCP3+3;区带Ⅲ以PSⅡ捕光复合物的聚集体LHCP1为主,区带Ⅱ和Ⅲ的吸收光谱中除了Chla外,还含有大量的Chlb和管藻黄素,是管藻黄素-Chla／b-蛋白质复合物;区带Ⅳ在PAGE中只显示一条带,光谱中有Chlb吸收肩峰,含有66和56kDa两种多肽,是较小的PSⅠ复合物CPⅠa。展开更多
Background: We investigated the immunohistochemical expression of Arp2/3 complex in 88 oral squamous cell carcinomas (OSCCs) and correlated it with the tumor type and grade. Materials and methods: This is a retrospect...Background: We investigated the immunohistochemical expression of Arp2/3 complex in 88 oral squamous cell carcinomas (OSCCs) and correlated it with the tumor type and grade. Materials and methods: This is a retrospective study of 88 cases of OSCCs (9 verrucous carcinomas, 4 carcinomas in situ, 37 well differentiated OSCCs, 32 moderately differentiated OSCCs, and 6 poorly differentiated OSCCs), stained with mouse monoclonal antibodies for Arp2 and Arp3. Staining was graded according to the extent of positivity by two observers. Results: Arp2 positivity was seen in 79 (89.77%) cases and Arp3 positivity in 78 (88.63%) cases. In positive cases, Arp2 expression was graded as (+) in 12.5%, (++) in 28.4% and (+++) in 48.86% of the cases;Arp3 was graded as (+) in 36.36%, (++) in 30.68% and (+++) in 21.59% of the cases. Conclusion: Expression of Arp2 and Arp3, indicative of the formation of the Arp2/3 complex, was seen in most OSCCs examined. Although no statistically significant correlation was found between the immunohistochemical expression of Arp2/Arp3 and tumor type or grade, the more common expression in invasive OSCCs than in non-invasive verrucous carcinomas and carcinomas in situ is indicative of its involvement in the biological behaviour of the lesions. The availability of Arp2/3 inhibitors that could be used in clinical practice warrants further study of the expression of Arp2/3 in OSCC.展开更多
Purified PSI complexes from Spinacia Oleracea L. were exposed to the strong light (PFD=2300μmol m-2s-1) for various period. Along with the illumination the photo-damage process of pigments and proteins of PSI complex...Purified PSI complexes from Spinacia Oleracea L. were exposed to the strong light (PFD=2300μmol m-2s-1) for various period. Along with the illumination the photo-damage process of pigments and proteins of PSI complexes was investigated using absorption, fluorescence, circular dichroism (CD) spectroscopy and SDS-PAGE. It was found from the optical absorption spectra that the maximal ab-sorbance of PSI complexes decreased and maximal peaks blue-shifted during the illumination, and the forth derivative spectra demonstrated that the absorbance decreasing at red region mainly resulted from the aborbance decreasing of the long wavelength Chla, implying that the long-wavelength Chla was readily to be bleached. The CD signals contributed by LHCI decreased more rapidly than other CD signals con-tributed by Chla and Carotenoid, indicating that the LHCI was more sensitive to light than core complexes. It was observed by SDS-PAGE that some small polypeptides of PSI complexes were damaged earlier than reaction展开更多
文摘采用聚丙烯酰胺凝胶电泳(PAGE)和蔗糖密度梯度超速离心方法分离了假根羽藻(Bryopsis corticulans)的色素-蛋白复合物,并对其特性进行分析。结果表明:采用PAGE分离得到7条色素-蛋白复合物带,分别是CP I a1、CP I a2、CP I、LHCP1、LHCP2、CPa、LHCP3+3,和2条游离色素(free pigment,FP)FCa、FC。用改进的不连续蔗糖密度梯度离心法分离到五条带。区带Ⅰ是FP;区带Ⅱ主要是小分子量的PSⅡ捕光复合物LHCP3+3;区带Ⅲ以PSⅡ捕光复合物的聚集体LHCP1为主,区带Ⅱ和Ⅲ的吸收光谱中除了Chla外,还含有大量的Chlb和管藻黄素,是管藻黄素-Chla／b-蛋白质复合物;区带Ⅳ在PAGE中只显示一条带,光谱中有Chlb吸收肩峰,含有66和56kDa两种多肽,是较小的PSⅠ复合物CPⅠa。
文摘Background: We investigated the immunohistochemical expression of Arp2/3 complex in 88 oral squamous cell carcinomas (OSCCs) and correlated it with the tumor type and grade. Materials and methods: This is a retrospective study of 88 cases of OSCCs (9 verrucous carcinomas, 4 carcinomas in situ, 37 well differentiated OSCCs, 32 moderately differentiated OSCCs, and 6 poorly differentiated OSCCs), stained with mouse monoclonal antibodies for Arp2 and Arp3. Staining was graded according to the extent of positivity by two observers. Results: Arp2 positivity was seen in 79 (89.77%) cases and Arp3 positivity in 78 (88.63%) cases. In positive cases, Arp2 expression was graded as (+) in 12.5%, (++) in 28.4% and (+++) in 48.86% of the cases;Arp3 was graded as (+) in 36.36%, (++) in 30.68% and (+++) in 21.59% of the cases. Conclusion: Expression of Arp2 and Arp3, indicative of the formation of the Arp2/3 complex, was seen in most OSCCs examined. Although no statistically significant correlation was found between the immunohistochemical expression of Arp2/Arp3 and tumor type or grade, the more common expression in invasive OSCCs than in non-invasive verrucous carcinomas and carcinomas in situ is indicative of its involvement in the biological behaviour of the lesions. The availability of Arp2/3 inhibitors that could be used in clinical practice warrants further study of the expression of Arp2/3 in OSCC.
基金This work was supported by the National Natural Science Foundation of China (Grant No. 39890390) the State Key Basic Research and Development Program (Grant No. G1998010100) and the Innovative Foundation of Laboratory of Photosynthesis Basic Research
文摘Purified PSI complexes from Spinacia Oleracea L. were exposed to the strong light (PFD=2300μmol m-2s-1) for various period. Along with the illumination the photo-damage process of pigments and proteins of PSI complexes was investigated using absorption, fluorescence, circular dichroism (CD) spectroscopy and SDS-PAGE. It was found from the optical absorption spectra that the maximal ab-sorbance of PSI complexes decreased and maximal peaks blue-shifted during the illumination, and the forth derivative spectra demonstrated that the absorbance decreasing at red region mainly resulted from the aborbance decreasing of the long wavelength Chla, implying that the long-wavelength Chla was readily to be bleached. The CD signals contributed by LHCI decreased more rapidly than other CD signals con-tributed by Chla and Carotenoid, indicating that the LHCI was more sensitive to light than core complexes. It was observed by SDS-PAGE that some small polypeptides of PSI complexes were damaged earlier than reaction
