[Objectives]To establish a TLC and content determination method of Pileostegia tomentellal,with umbelliferone as the indicator component.[Methods]TLC identification was performed by silica gel G thin layer plate with ...[Objectives]To establish a TLC and content determination method of Pileostegia tomentellal,with umbelliferone as the indicator component.[Methods]TLC identification was performed by silica gel G thin layer plate with n-hexane-ethyl acetate(4:3)as the developing agent,and the plate was examined by UV lamp(365 nm).The umbelliferone content was determined by HPLC:Inertsil ODS-3 C 18 column(4.60 mm×250 mm,5μm);mobile phase acetonitrile-0.2%phosphoric acid gradient elution;detection wavelength 320 nm,flow rate 1.0 mL/min,column temperature 30℃,injection volume 10μL.[Results]The chromatogram of P.tomentellal showed the same color spot in the same position as that of reference medicinal material,and the spot was clear with good specificity.Umbelliferone showed a good linear relationship when the injection volume was 2.63-131.27μg/mL(R^(2)=0.9997).The average recovery of umbelliferone in the low,middle and high adding groups of P.tomentellal was 99.57%and the RSD was 2.15%.[Conclusions]The method can effectively identify Yao medicine P.tomentellal and accurately determine the content of umbelliferone in medicinal materials,which will provide a scientific basis for the development and utilization of medicinal resources of Yao medicine P.tomentellal.展开更多
[Objectives]To establish a method for quality analysis of Medicine Pileostegia tomentella Hand.-Mazz.[Methods]According to the testing method of Chinese Pharmacopoeia(2020 Edition),the water content,total ash and alco...[Objectives]To establish a method for quality analysis of Medicine Pileostegia tomentella Hand.-Mazz.[Methods]According to the testing method of Chinese Pharmacopoeia(2020 Edition),the water content,total ash and alcohol-soluble extract of P.tomentella Hand.-Mazz.were determined.[Results]10 batches of P.tomentella Hand.-Mazz.from different habitats and in different collection time in Guangxi all had the same plant morphology and medicinal properties.The experimental results showed that the water content was 7.92%-10.35%,the total ash content was 5.53%-6.62%,the acid-insoluble ash content was 0.59%-0.76%,and the extract content was 11.63%-16.12%.[Conclusions]The identification method in this study can be used for the quality analysis of P.tomentella Hand.-Mazz.,and the experimental results can provide a scientific basis for the development and utilization of P.tomentella Hand.-Mazz.and the establishment of its quality standard.展开更多
Lung cancer is the leading cause of cancer death and the most common malignant tumor, the long-term survival of which has stagnated in the past several decades.Pileostegia tomentella Hand.Mazz is a traditional Chinese...Lung cancer is the leading cause of cancer death and the most common malignant tumor, the long-term survival of which has stagnated in the past several decades.Pileostegia tomentella Hand.Mazz is a traditional Chinese medicine called "Zhongliuteng"(ZLT) in the pharmacopeia, which has been proved to possess a potent anti-tumor effect on various cancers.In this study, the effects of ZLT N-butanol extraction(ZLTN) and ZLT ethyl acetate extraction(ZLTE) on the viability of non-small cell lung cancer cell(NSCLC) lines H1299 and A549 were evaluated.Here, we firstly reported that ZLTE significantly inhibited H1299 cells growth without affecting the release of lactate dehydrogenase(LDH).In addition, ZLTE induced caspase-dependent apoptosis in a concentration-dependent manner and increased the expression cleaved-PARP and decreased pro-caspase-3, pro-caspase-7, pro-caspase-8, and pro-caspase-9.Moreover, ZLTE increased the level of cellular reactive oxygen species(ROS) in H1299 cells to lead to apoptosis,which was reversed by N-acetyl-cysteine(NAC).Taken together, our results revealed that ZLTE induced caspase-dependent apoptosis via ROS generation, suggesting that ZLTE is a promising herbal medicine for the treatment of NSCLC.展开更多
基金Supported by Self-funded Research Project of Administration of Traditional Chinese Medicine of Guangxi Zhuang Autonomous Region(GXZYA20220171)Young and Middle-aged Teachers Research Basic Ability Improvement Project of Colleges and Universities in Guangxi(2022KY0307)+5 种基金General Project of Guangxi University of Chinese Medicine(2022MS038)"Qingmiao Project"Talent Cultivation Program of Guangxi International Zhuang Medical Hospital(2022001)Key Project of Guangxi International Zhuang Medical Hospital(GZ2021010)High-level TCM Key Discipline(Zhuang Medical Science)Construction Project of State Administration of Traditional Chinese Medicine(zyyzdxk-2023165)Key Research and Development Project of Guangxi Provincial Department of Science and Technology(GK AB21196057)High-level Talent Cultivation Innovation Team Funding Project of Guangxi University of Chinese Medicine(2022A008).
文摘[Objectives]To establish a TLC and content determination method of Pileostegia tomentellal,with umbelliferone as the indicator component.[Methods]TLC identification was performed by silica gel G thin layer plate with n-hexane-ethyl acetate(4:3)as the developing agent,and the plate was examined by UV lamp(365 nm).The umbelliferone content was determined by HPLC:Inertsil ODS-3 C 18 column(4.60 mm×250 mm,5μm);mobile phase acetonitrile-0.2%phosphoric acid gradient elution;detection wavelength 320 nm,flow rate 1.0 mL/min,column temperature 30℃,injection volume 10μL.[Results]The chromatogram of P.tomentellal showed the same color spot in the same position as that of reference medicinal material,and the spot was clear with good specificity.Umbelliferone showed a good linear relationship when the injection volume was 2.63-131.27μg/mL(R^(2)=0.9997).The average recovery of umbelliferone in the low,middle and high adding groups of P.tomentellal was 99.57%and the RSD was 2.15%.[Conclusions]The method can effectively identify Yao medicine P.tomentellal and accurately determine the content of umbelliferone in medicinal materials,which will provide a scientific basis for the development and utilization of medicinal resources of Yao medicine P.tomentellal.
基金Supported by 2022 Guangxi University Young and Middle-aged Teachers Basic Scientific Research Ability Improvement Project(2022KY0307)Key R&D Project of Guangxi Science and Technology Department(GuiKe AB21196057)+5 种基金General Project of Guangxi University of Chinese Medicine in 2022(2022MS038)Self-Raised Scientific Research Fund Project of Guangxi Zhuang Autonomous Region Administration of Traditional Chinese Medicine(GXZYA20220171)Key Project of Guangxi International Zhuang Medicine Hospital(GZ2021010)Zhuang Medicine Study,A Key Discipline of Traditional Chinese Medicine(Ethnopharmacology)in National Administration of Traditional Chinese Medicine during the 12 th Five-Year PlanHigh-level Talent Cultivation Innovation Team Fund Project of Guangxi University of Chinese Medicine(2022A008)Talent Training Project of"Young Talent Project"in Guangxi International Zhuang Medicine Hospital(2022001).
文摘[Objectives]To establish a method for quality analysis of Medicine Pileostegia tomentella Hand.-Mazz.[Methods]According to the testing method of Chinese Pharmacopoeia(2020 Edition),the water content,total ash and alcohol-soluble extract of P.tomentella Hand.-Mazz.were determined.[Results]10 batches of P.tomentella Hand.-Mazz.from different habitats and in different collection time in Guangxi all had the same plant morphology and medicinal properties.The experimental results showed that the water content was 7.92%-10.35%,the total ash content was 5.53%-6.62%,the acid-insoluble ash content was 0.59%-0.76%,and the extract content was 11.63%-16.12%.[Conclusions]The identification method in this study can be used for the quality analysis of P.tomentella Hand.-Mazz.,and the experimental results can provide a scientific basis for the development and utilization of P.tomentella Hand.-Mazz.and the establishment of its quality standard.
基金support by the Key Laboratory for Pharmacology Research of Guangxi TCM(KJT14038,14-045-12-K5)the Project of Guangxi Overseas “100 persons plan”High-level Expert。
文摘Lung cancer is the leading cause of cancer death and the most common malignant tumor, the long-term survival of which has stagnated in the past several decades.Pileostegia tomentella Hand.Mazz is a traditional Chinese medicine called "Zhongliuteng"(ZLT) in the pharmacopeia, which has been proved to possess a potent anti-tumor effect on various cancers.In this study, the effects of ZLT N-butanol extraction(ZLTN) and ZLT ethyl acetate extraction(ZLTE) on the viability of non-small cell lung cancer cell(NSCLC) lines H1299 and A549 were evaluated.Here, we firstly reported that ZLTE significantly inhibited H1299 cells growth without affecting the release of lactate dehydrogenase(LDH).In addition, ZLTE induced caspase-dependent apoptosis in a concentration-dependent manner and increased the expression cleaved-PARP and decreased pro-caspase-3, pro-caspase-7, pro-caspase-8, and pro-caspase-9.Moreover, ZLTE increased the level of cellular reactive oxygen species(ROS) in H1299 cells to lead to apoptosis,which was reversed by N-acetyl-cysteine(NAC).Taken together, our results revealed that ZLTE induced caspase-dependent apoptosis via ROS generation, suggesting that ZLTE is a promising herbal medicine for the treatment of NSCLC.
