Genetic diversity and genetic differentiation of natural populations of Pinus kesiya var. langbinanensis were examined by means of electrophoresis technique. Analysis of 9 enzyme systems including 16 loci showed that ...Genetic diversity and genetic differentiation of natural populations of Pinus kesiya var. langbinanensis were examined by means of electrophoresis technique. Analysis of 9 enzyme systems including 16 loci showed that all the three natural popu-lations of the pine were high in genetic diversity but low in inter-population genetic differentiation. The proportion of polymorphic loci is 0.667, with each locus holding 2.13 alleles, averagely. The average expected and observed heterozygosity was 0.288 and 0.197, respectively. The gene differentiation among populations was 0.052, but the mean genetic distance was only 0.015.展开更多
In the present study, the genetic distances between 85 superior clones from seven natural populations of Pinus kesiya var. langbianensis were analyzed by RAPD-PCR assays, and then, a UPGMA cluster diagram was establis...In the present study, the genetic distances between 85 superior clones from seven natural populations of Pinus kesiya var. langbianensis were analyzed by RAPD-PCR assays, and then, a UPGMA cluster diagram was established based on the genetic distances to reveal their genetic relationship. In addition, specific markers of elite clones were identified by carefully comparing the fingerprints of the 85 clones. The results showed that the 85 P. kesiya clones were divided into three groups at a genetic distance of 0.59. Two specific markers that were able to distinguish some elite clones from others were identified. The results may provide a molecular basis for the identification and selection of elite clones of Pinus kesiya var. langbianensis.展开更多
The authors investigated the genetic diversity of 29 natural populations representing Pinus yunnanensis Franch. and its two close relatives, P. densata Mast. and P. kesiya Royle ex Gordn. var. langbianensis (A Chey.) ...The authors investigated the genetic diversity of 29 natural populations representing Pinus yunnanensis Franch. and its two close relatives, P. densata Mast. and P. kesiya Royle ex Gordn. var. langbianensis (A Chey.) Gaussen. Horizontal starch gel electrophoresis was performed for macrogametophytes collected from populations in Yunnan, Sichuan and Guangxi. Allozyme data for 33 loci of 14 enzymes demonstrated high levels of genetic variation at both population and species levels in comparison with other conifers, with the mean values for populations being P=0.694, A =2.0 and He =0.145 for P. yunnanensis; P=0.714, A=2.0 and He =0.174 for P. densata ; and P=0.758, A=2.1 and He =0.184 for P. kesiya var. langbianensis. Based on Wright’s F _statistics, the fixation index of P. yunnanensis, P. densata and P. kesiya var. langbianensis were 0.101, 0.054 and 0.143, respectively, indicating that the populations were largely under random mating. Based on Nei’s genetic distance, the genetic differentiation was not obvious among the three species (i.e. the genetic distance was less than 0.075). Because of the wider distribution of P. yunnanensis with greater variety of habitats, it was shown that the genetic differentiation among the P. yunnanensis populations was larger than that of the populations of the other two species. According to morphological, geographic and allozymic evidences, the authors suggested that the three species be better treated as varieties under a single species. In addition, the extensive gene flow among the three pine species resulted in great genetic diversity and evolutionary potential. Also, high level of genetic variation of P. yunnanensis provides important basis for its genetic improvement and breeding in future.展开更多
基金his article was supported by the Natural Science Founda-tion of Yunnan Province (No.98C017Q).
文摘Genetic diversity and genetic differentiation of natural populations of Pinus kesiya var. langbinanensis were examined by means of electrophoresis technique. Analysis of 9 enzyme systems including 16 loci showed that all the three natural popu-lations of the pine were high in genetic diversity but low in inter-population genetic differentiation. The proportion of polymorphic loci is 0.667, with each locus holding 2.13 alleles, averagely. The average expected and observed heterozygosity was 0.288 and 0.197, respectively. The gene differentiation among populations was 0.052, but the mean genetic distance was only 0.015.
基金Supported by Special Fund of National Development and Reform Commission of China for Bio-breeding High Technology Industrialization
文摘In the present study, the genetic distances between 85 superior clones from seven natural populations of Pinus kesiya var. langbianensis were analyzed by RAPD-PCR assays, and then, a UPGMA cluster diagram was established based on the genetic distances to reveal their genetic relationship. In addition, specific markers of elite clones were identified by carefully comparing the fingerprints of the 85 clones. The results showed that the 85 P. kesiya clones were divided into three groups at a genetic distance of 0.59. Two specific markers that were able to distinguish some elite clones from others were identified. The results may provide a molecular basis for the identification and selection of elite clones of Pinus kesiya var. langbianensis.
文摘The authors investigated the genetic diversity of 29 natural populations representing Pinus yunnanensis Franch. and its two close relatives, P. densata Mast. and P. kesiya Royle ex Gordn. var. langbianensis (A Chey.) Gaussen. Horizontal starch gel electrophoresis was performed for macrogametophytes collected from populations in Yunnan, Sichuan and Guangxi. Allozyme data for 33 loci of 14 enzymes demonstrated high levels of genetic variation at both population and species levels in comparison with other conifers, with the mean values for populations being P=0.694, A =2.0 and He =0.145 for P. yunnanensis; P=0.714, A=2.0 and He =0.174 for P. densata ; and P=0.758, A=2.1 and He =0.184 for P. kesiya var. langbianensis. Based on Wright’s F _statistics, the fixation index of P. yunnanensis, P. densata and P. kesiya var. langbianensis were 0.101, 0.054 and 0.143, respectively, indicating that the populations were largely under random mating. Based on Nei’s genetic distance, the genetic differentiation was not obvious among the three species (i.e. the genetic distance was less than 0.075). Because of the wider distribution of P. yunnanensis with greater variety of habitats, it was shown that the genetic differentiation among the P. yunnanensis populations was larger than that of the populations of the other two species. According to morphological, geographic and allozymic evidences, the authors suggested that the three species be better treated as varieties under a single species. In addition, the extensive gene flow among the three pine species resulted in great genetic diversity and evolutionary potential. Also, high level of genetic variation of P. yunnanensis provides important basis for its genetic improvement and breeding in future.
