Optimum conditions for pure culture of major ectomycorrhizal fungi obtained from Pinus sylvestris var. mongolica plantations in southeastern Keerqin sandy lands, China

The effects of medium, pH, water potential and temperature on the culture for three pure strains （Lactarius deliciosus, Boletus edulis and Lactarius insulsus） of ectomycorrhizal fungi from plantation forests of Mongolian pine （Pinus sylvestris var. mongolica） on sandy lands were observed to obtain the optimum conditions for the growth of ectomycorrhizal fungi. The results indicated that the three ectomycorrhizal fungi could grow well in the mediums containing natural components, such as vitamin, pine juice and yeast powder, pH had a slight effect on the growth of the three ectomycorrhizal fungi, and the optimum pH values were 6.0 for L. deliciosus, 5.0 for B. edulis, respectively. However, L. insulsus had a wide pH range, and it grew better than the other two strains in neutral and light alkalescent mediums. Water potential （produced by Polyethylene Glycol, PEG） had significant effects on the ecological adaptability for the tested three fungi strains. All of the three stains grow better at lower PEG concentration （100 g PEG.kg^-1 H2O）. The best water potential was 10% PEG concentration for all of the three stains. Temperatures, especially high temperatures induced the fungi death. The optimum temperature for the growth of ectomycorrhizal fungi was 25-28℃ for all of the three stains.

Effects of silvicultural treatments on mechanical properties of Pinus sylvestris var.mongolica plantations

A study was conducted to determine the influences of initial planting densities, thinning intensities, exposures and slope sites on physical property （wood density） and mechanical properties such as modulus of elasticity （MOE）, bending strength, impact strength, compression strength along grain and hardness） of Pinus sylvestris var. mongolica plantation in Mao＇ershan Forest Farm, Northeast China. Results show that the different initial planting densities （1.5 m×1.0 m, 1.5 m×2.0 m and 1.5 m×2.5 m） had significant effects on wood density and MOE, and the highest mean wood density and indexes of mechanical properties occurred in the stand with an initial planting density of 1.5 m×1.0 m. The indexes of mechanical properties such as hardness of end, bending strength, MOE and compression strength along grain of wood increased after mild thinning, but decreased after violent thinning. The exposures （sunny slope and shady slope） had a significant effect on MOE, and the highest mean MOE occurred on sunny slope. The slope sites （upper site and lower site） had a significant effect on wood density and main mechanical properties, except hardness. The highest mean wood density and mechanical properties occurred at lower site.

Promoting the Growth of Pinus sylvestris var.mongolica Seedlings and Improving Rhizosphere Fungal Community Structure through Interaction between Trichoderma and Ectomycorrhizal Fungi

In this study,pot experiments were conducted on the seedlings of Pinus sylvestris var.mongolica to study the influence of Trichoderma(Trichoderma harzianum E15)and Ectomycorrhizal fungi(Suillus luteus N94)on the growth of these seedlings.In particular,the effects of these fungi on the fungal community structure in the rhizosphere soil of the seedlings were investigated.Inoculation with Trichoderma harzianum E15 and Suillus luteus N94 significantly(P<0.05)promoted the growth of the Pinus sylvestris seedlings.The non-metric multidimensional scaling(NMDS)results indicated a significant difference(P<0.05)between the fungal community structures in the rhizosphere soil of the annual and biennial seedlings.In the rhizosphere soil of annual seedlings,the main fungi were Ascomycota,Basidiomycota,Zygomycota.Ascomycota,Basidiomycota,Mortierellomycota,and p-unclassified-k-Fungi were the main fungi in the rhizosphere soil of biennial seedlings.The dominant genus in the rhizosphere soil and a key factor promoting the growth of the annual and the biennial seedlings was Trichoderma,Suillus,respectively.Both of them were negatively correlated with the relative abundance of microbial flora in the symbiotic environment.Trichoderma had a significant promoting effect on the conversion of total phosphorus,total nitrogen,ammonium nitrogen,nitrate nitrogen,and the organic matter in the rhizosphere soil of the seedlings,while Suillus significantly promoted the conversion of organic matter and total phosphorus.

Natural regeneration characteristics of Pinus sylvestris var. mongolica forests on sandy land in Honghuaerji, China

Natural regeneration in Mongolian pine, Pinus sylvesttis var. mongolica, forest at Honghuaerji of China （the original of the natural Mongolian pine, forest on sandy land） was studied in 2004. The total mean values of regeneration indexes were higher in mature stands （more than 80% individual stems were older than 50 years）, the maximum of regeneration index reached 29 seedlings, m^ 2, with lowest values in the younger stand, e.g., in 32-year old and 43-year old stands. The stand age was an important factor determining the natural regeneration, which was the best in the older stands in this investigation （e.g. about 80-year old）. The regeneration index seemed not to be closely in relation to canopy openness although Mongolian pine is a photophilic tree species. In each type of gaps, natural regeneration was very well. Regeneration indexes were satisfactory at the south and east edges in the circle gaps; and at the east edge of the narrow-square gaps. Results indicated that Mongolian pine, seedlings could endure shading understory, but it would not enter the canopy layer without gap or large disturbance, e.g., fire, wind/snow damage or clear cutting etc. These results may provide potentially references to the management and afforestation of Mongolian pine, plantations on sandy land in arid and semi-arid areas. Researches such as the comprehensive comparisons on regeneration, structure and ecological conditions and so on between natural Mongolian pine, forests and plantations should be conducted in the future.

Ectomycorrhizal fungus enhances drought tolerance of Pinus sylvestris var.mongolica seedlings and improves soil condition

Mongolian pine is an important afforestation species widely used for ecological management in northeast China. The environment in this region is very unstable and the flora are regularly subjected to drought stress.This paper reports on the influence of inoculation with the Suillus luteus on seedlings under different water conditions.Both inoculated and non-inoculated ectomycorrhizal fungi(ECMF)-S. luteus seedlings were maintained under wellwatered or water-stress conditions for 3 months. The S.luteus colonization rate under water stress was higher than that in well-watered conditions. Under water stress, inoculated seedlings had greater growth than non-inoculated seedlings. In addition, under water stress, S. luteus-inoculated seedlings had greater superoxide dismutase and peroxidase activity, higher soluble protein content, lower proline content, and lower malondialdehyde content than non-inoculated seedlings. S. luteus colonization increased the rhizosphere soil-enzyme activity and the rhizosphere soil nutrition content under both well-watered and water-stress conditions. Given the positive impact on seedling growth and physiology, S. luteus shows potential for use in the arid and semi-arid regions of northeast China for afforestation.

Comparison of foliar nutrient concentrations between natural and artificial forests of Pinus sylvestris var. mongolica on sandy land, China

In order to examine the causes of degradation of Pinus sylvestris var. mongolica plantations on sandy land, the foliar concentrations of N, P, K and C were analyzed and compared between the field grown P. sylvestris var. mongolica trees from two provenances （natural forests and plantations）. The results indicated that natural tree needles had lower N, P and C concentrations, and higher K concentrations than those of plantation tree needles. For plantation tree needles, ratios of N： P, P. K and N： K increased with tree age before 45 years old; but they were not clear for the natural tree needles. Compared with the conclusions reported on Pinus spp., we found that the foliar N and P concentrations were in the optimal range for both natural and plantation tree needles. This result suggested that N or P might not be the absolute limit factors in plant nutrient for P sylvestris var. mongolica on sandy land. However, foliar K concentrations in both natural and plantation tree needles were much lower than those reported on Pinus spp. （〉4.80 g kg-1）.The N： P ratio of natural needles was in the adequate ranges, but N： P ratio of plantation needles was out of the adequate ranges. These results indicated that there was a better balanced nutrition status in the natural forest than in the plantations. If only considering the foliar nutrient concentrations of P sylvestris var. mongolica from different provenances, it might be concluded that the degradation phenomenon of P. sylvestris var. mongolica plantations was not induced by nutrition deficiency of absolute nutrients of N and P, but might be induced by other mineral nutrients or by the effectiveness of N and P nutrients. The unbalanced nutrition status and relatively quick decomposition of needles in the plantations might also contribute to the degradation.

COMPARATIVE STUDY ON DROUGHT RESISTANCE OF LARIX OLGENSIS HENRY AND PINUS SYLVESTRIS VAR.MONGOLICA(Ⅰ)──THE INFLUENCE OF SOIL WATER CONTENT ON SEEDLINGS PLANTING IN FLOWERPOT

Water potential (φ w .) and net photosynthetic rate (Pn) of Larix olgensis and Pinns.sylvestris var. mongolica deercased with the deerease of soil water content φw and Pn of L.olgensis changed hardly during the first 9 davs after stopping watering, then deereased sharply at the 10th dav Pn of P sylvestris var mongolica deereased slightly during the lirst 8 days, then deereased sharply at the 9th day Their respiration rate, chlorophyll content and their a/b ratio changed hardly. The tollowing 3 conclusions were obtained and discussed exhaustively . (Ⅰ) φ w can be used to direct watering as a sensitive index of judging whether L. olgensis and P.sylvestris var. mongolica lacking water (2 )The deereasc of Pn of L. olgensis and P. sylvestris var. mongolica when drought had nothing to do with chlorophyll. (3) P. sylvestris var. mongolica had morphological drought resistance . while L,olgensis had physiological drought resistance, and their drought resistance was discnssed comparatively first time.

Effect of Three Treatment Measures on Harmless Seedling Raising of Pinus sylvestris var.mongolica Litv.

Three treatment measures for raising seedlings by sowing, Le. covering seeds with two layers of forest soil, culturing seedlings under full sunshine and timely sowing in late autumn, were adopted in this study. The results indicated that the three treatments were of good prevention effects on sheath blight or seedling blight, sunscald and rat damage in Pinus sylvestris var. mongolica, could avoid the pollution of agrochemicals to soil and environment as well as decrease cost for raising seedlings, and thus could promote the development of bio-agrochemicals or bio-fungicides with high efficiency and no pollution for resisting the pathogens of sheath blight.

Dynamics of Soil CO_2 Profiles of Pinus sylvestris var. sylvestriformis Seedlings Under CO_2 Concentration Doubled

The gas_well system permanently installed in the soil was adopted for studying the dynamic relationship between CO 2 profiles and seedling root growth of Pinus sylvestris var. sylvestriformis (Takenouchi) Cheng et C. D. Chu. The study was conducted in the Open Research Station of Changbai Mountain Forest Ecological System, The Chinese Academy of Sciences from 1999 to 2001. Four treatments were arranged in the rectangular open_top chambers (OTCs): ambient CO 2+no_seedling, 700 μmol/mol CO 2+no_seedling, ambient CO 2 +seedlings, 700 μmol/mol CO 2+seedlings. By collecting and analyzing soil gas synchronously, it was found that the dynamics of CO 2 profiles were related to the biological activity of seedlings. There were more roots distributed in the top soil and the boundary layer across soil and sand, which made more contributions to the CO 2 profiles due to respiration root. Compared with the ambient CO 2, elevated CO 2 led to the peak of CO 2 concentration distribution shifted from soil surface layer to the boundary layer as seasonally growing of seedling roots. It is suggested the gas_well system is an inexpensive, non_destructive and relatively sensitive method for study of soil CO 2 concentration profiles.

Disease Law and Forecasting of the Needle Blight ofPinus sylvestris var. mongolica

Fixed quadrates were established in different stands. In continued six years, the occurring period, occurring amounts and the relation between epidemic disease and environmental factors were investigated according to spraying lawsof spores and accounting measures of disease ranking. The occuring peak period of the disease was from the last ten days ofMay to the second ten days of June. The epidemic period was from the last ten days of June to the second ten days of Julyand the initial decease period was from the last ten days of July to the beginning of September. The change of the diseasedepended on air temperature, relative humidity and precipitation. A multiple linear regression model was established usingcomputer, which can predict the disease index(Y) of 10 days later, with more than 95% reliability

Spatial distribution of needle bright disease of Pinus sylvestris var.mongolica

The spatial distribution pattern of needle bright disease was mathematically studied. The results showed that it is tally with negative binomial distribution. The infected area and damaged amount can be forecasted with this spatial distribution pattern. Through the study on probability distribution of spatial points for single tree, the method and equations for calculation of the disease index of whole forest belt were determined.

INTRASPECIFIC GEOGRAPHIC VARIATION AND PRIMARY SELECTION ON THE BEST PROVENANCE OF PINUS SYLVESTRIS VAR. MONGOLICA

This paper summarizes the result from the provenance test of eight years old Pinus sylvestris var. mongolica in Liangshui Natural Rcserve comprehensivcly. The main contents include:(1) the geographic variation of the growth characters of Pinus sylvestris var.mongolica.tends to negative change gradually at latitude and has obvious climatic nature, (2) no significant difference exists in the morphological adaptability and resistant characteristics and so on; (3)on the basis of the geographic variation study on the growth characteristics, the provcnances of Pinus sylvestris var. mongolica were classificd into two geographic populations and two subpopulations as follows:the northern population of the Daxing'an Mountain (Ⅰ) and the southern population of the Daxing'an Mountain as wcll as the Xiaoxinglan Mountain (Ⅱ),which includcs Honghuarii subpopulation and Kalunshan subpopulation (Ⅱ-2). (4) the height growth rhythm was analyzed according to the divided population and subpopuIation; (5) the best provcnance was selected for the Liangshui Natural Rescrve and its neighborhood accord ing to the results from SSR test of the growth characteristics (the breast height diameter and height of eight year old trces), the possibility of the carly provcnance sclection was also verified by the method of order correlation analysis on the height growth of three. five, scvcn and eight years old provenances.

Structure and functions of Pinus sylvestris var. Sylvestriformis plantation ecosystem

Changbai pine (Pinus sylvestris var.Sylvestriformis) is an endemic and important tree species in Changbai Mountain. There were only 63 plant species in Changbai pine plantation, where hemicryptophyte was dominant(39.68%). Simpson diversity index was 0.87, Shannon-Wiener diversity index was 2.96, and evenness index was 0.82. Community structure were divided into three layers: tree layer, shrub layer and herb layer. The total biomass and net production were 111.982 t/hm2 and 8942.80 kg/(hm2 a) respectively. The total biomass for tree, shrub and herb layers were 106.150, 2.230, 2.264 t/hm2, accounting for 94.79%, 1.99%, and 2.02%, respectively, and net production for those were 7465, 223, and 1182 kg/(hm2 a), accounting for 83.47%, 2.49%, and 13.22% of the total respectively. The nutrient content in various organs is in the order of needle> branch> root> bark> trunk, For the assimilated organ, the nutrient content is in the order of N> K> Ca> P> Mg, and that in absorption organ is in the order of Ca> N> K> P> Mg. For the whole plantation ecosystem, nutrient content is in the order of soil> litter> herb layer> shrub layer> tree layer. Nutrient storage and its accumulation rate in tree layer take up 88.79% and 76.43% of the total, respectively.

Climatic response of Pinus sylvestris var. mongolica tree-ring width and precipitation reconstruction for the northern Greater Higgnan Mountains, China, since 1720

August-June precipitation has been reconstructed back to AD 1720 for the northern Greater Higgnan Mountains, China, by use ofPinus sylvestris var. mongolica tree-ring width. The reconstruction explains the variance of 39% in observed precipitation from 1973 to 2008. Some extremely dry/wet signals in historical documents and other precipitation reconstructions in previous studies are precisely captured in our reconstruction. Wet periods occurred during the periods of 1730 to 1746, 1789 to 1812, 1844 to 1903, 1920 to 1930, 1942 to 1961, and 1985 to 1998; while periods of 1747 to 1788, 1813 to 1843, 1904 to 1919, 1931 to 1941, and 1962 to 1984 were relatively dry. Power spectral and wavelet analyses demon- strated the existence of significant 24-year, 12-year, and 2-year cycles of variability.

科尔沁沙地樟子松(Pinus sylvestris var.mongolia)人工林土壤真菌群落结构和功能特征

系统解析科尔沁沙地不同林龄樟子松(Pinus sylvestris var.mongolia)人工林土壤真菌群落结构和功能特征及其与土壤理化性质的关系,为樟子松人工林可持续经营管理提供理论依据。以科尔沁沙地幼龄林(13 a)、中龄林(22 a)、近熟林(34 a)、成熟林(41 a)和过熟林(55 a)樟子松人工林为研究对象,采用野外调查和分子生物学相结合的方法,利用Illumina MiSeq高通量测序和FUNGuild平台鉴定分析樟子松人工林土壤真菌群落。结果表明:(1)从科尔沁沙地樟子松人工林土壤样品中共获得922个OTUs,隶属于14门25纲67目98科254属。子囊菌门Ascomycota和担子菌门Basidiomycota占绝对优势,优势属为美口菌属Calostoma和糙缘腺革菌属Amphinema。(2)随林龄增加,共生营养型真菌及主要功能群外生菌根真菌占比均呈先减小后增大趋势,且成熟林占比最小;腐生营养型真菌及主要功能群未定义腐生真菌占比均呈先增大后减小趋势,且成熟林占比最大;病理营养型真菌分布较为均匀。(3)随林龄增加,土壤真菌网络呈先简单后复杂趋势,近熟林最为简单,过熟林最为复杂,而成熟林次之。(4)土壤真菌群落结构主要受土壤有机质、pH、碱解氮和硝态氮的驱动,腐生营养型真菌相对丰度与土壤全磷、pH分别呈显著正、负相关性(P<0.05),腐生营养型真菌相对丰度与土壤有机质呈极显著正相关(P<0.01)。林龄和土壤理化性质对科尔沁沙地樟子松人工林土壤真菌群落构建起到关键作用。樟子松幼龄林至近熟林阶段,共生营养型真菌大量富集占据主导地位,有利于林木快速生长;成熟林和过熟林阶段,土壤真菌群落结构相似,腐生营养型真菌发挥主导作用,且真菌网络愈加复杂并趋于稳定,有利于提高林木对外部环境抵抗能力。研究结果有助于深入理解科尔沁沙地樟子松人工林土壤真菌生态功能,并为从土壤真菌视角评价和理解林木和土壤健康提供科学依据。

Effects of peat and weathered coal on the growth of Pinus sylvestrisvar. mongolica seedlings on aeolian sandy soil

The experiment was conducted at the Ganqika Sandy Land Ecological Station in Ke抏rqinzuoyihouqi County, Inner Mongolia, in a growing season from April 28 to October 28, 2001. Peat and weathered coal were added to the aeolian sandy soil in different ratios. Two-year-old Pinus sylvestris var. mongolica seedlings and plastic pots were used in the experiment. The experimental results indicated that: 1) the peat and weathered coal could significantly improve the physical and chemical prop-erties of aeolian sandy soil, and thus promoted the growth of seedlings; 2) the effect of peat on seedling growth, including height, base diameter, root length and biomass, presented an order of 8%>10%>5%>2%>0 in terms of peat contents, and the effect of weathered coal on seedling growth presented an order of 5%>8%>10%>2%>0 in terms of weathered coal contents for height and basal diameter, 5%>8%>2% >10%>0 for root length, and 5%>2%>8% >10%>0 for biomass; 3) the effects of peat were generally greater than that of weathered coal. Meanwhile, 8% peat was the best treatment to promote the growth of P. sylvestris var. mongolica seedlings.

Dynamic Simulation of Carbon Sequestration by Pinus sylvestris var. mongolica

Based on the functional process of carbon sequestration of forest, we categorize carbon sequestration in two parts： carbon capture and carbon storage. Here,Pinus sylvestris var.mongolica was used to simulate the dynamic changing process of carbon sequestration of forest in a century using tree growth formula and the cost of carbon capture and sequestration. We found that the total carbon captured and stored by 100-year-old P. sylvestris var.mongolica are both 966 kg C tree^-1. On the assumption that the current year is the planting year, the present total carbon sequestration value （capital value） of 100-year-old P. sylvesrtis var.mongolica is 54.78 USD tree^-1 at the discount rate of 3%, with the present total carbon capture value of 18.92 USD tree^-1 and carbon storage value of 35.86 USD tree^-1. The dynamic process of the annual value of carbon capture is ilustrated by an upside-down U shape curve, while that of carbon storage service folows an S shape curve. The combination of the dynamic processes of carbon capture value and carbon storage value represent the changing process of carbon sequestration value, which appears to be an S shape curve.

沙地樟子松( Pinus sylvestris var. mongolica )物候特征及其对气候的响应

物候反映植被对环境变化的响应。沙地樟子松(Pinus sylvestris var.mongolica)被广泛引种栽植于中国北方风沙区,但引种区气候条件差异对沙地樟子松物候的影响仍然尚不明确。基于2001—2020年MOD13Q1数据和气象数据,分别采用最大斜率法和趋势分析方法提取沙地樟子松植被遥感物候信息并比较物候时空动态特征差异,以偏最小二乘回归方法分析沙地樟子松遥感物候对气象因子变化的响应。结果表明:(1)呼伦贝尔、科尔沁、毛乌素沙地樟子松生长季分别始于第100—150、120—140、100—140天;生长季分别止于第300—340、270—315、260—310天;生长季长度分别为170—220、140—180、150—200 d。(2)呼伦贝尔、科尔沁、毛乌素沙地樟子松生长季始期显著提前(P<0.05),变化率分别为-15.7、-5.7、-13.9 d/10a;科尔沁、毛乌素沙地樟子松生长季末期显著提前(P<0.05),变化率分别为-11.7、-16.8 d/10a;呼伦贝尔沙地樟子松生长季长度显著延长(P<0.05),变化率为9.8 d/10a。(3)呼伦贝尔沙地樟子松生长季始期提前主要受上年8月气温降低和3月降水减少影响,生长季末期提前主要受1月降水减少影响;科尔沁沙地樟子松生长季始期和末期提前分别受上年7月降水减少和5月降水增加影响;毛乌素沙地生长季始期提前主要受3月气温升高和上年12月降水减少影响,生长季末期提前主要受9月气温上升和上年12月降水减少影响。研究结果可为深入理解沙地樟子松物候特征和人工林经营管理提供理论依据。

Influence of CO_2 Doubling on Water Transport Process at Root/Soil Interface of Pinus sylvestris var. sylvestriformis Seedlings

Water transport at the root/soil interface of 1 year old Pinus sylvestris Linn. var. sylvestriformis (Takenouchi) Cheng et C. D. Chu seedlings under CO 2 doubling was studied by measuring soil electric conductance to survey soil water profiles and comparing it with root distribution surveyed by soil coring and root harvesting in Changbai Mountain in 1999. The results were: (1) The profiles of soil water content were adjusted by root activity. The water content of the soil layer with abundant roots was higher. (2) When CO 2 concentration was doubled, water transport was more active at the root/soil interface and the roots were distributed into deeper layer. It was shown in this work that the method of measuring electric conductance is an inexpensive, non_destructive and relatively sensitive way for underground water transport process.

半干旱地区果用型红松优良无性系选择

为筛选出吉林省西部半干旱地区优良红松(Pinus koraiensis)无性系种质资源,以30个嫁接红松无性系为材料,利用樟子松(Pinus sylvestris var. mongholica Litv.)幼树高枝嫁接红松,营建红松无性系对比试验林,对其进行多年观测。以单株产种量、千粒重、出仁率为综合评价指标,从中筛选出10个种子产量高、质量好的果用型优良红松无性系,其平均单株产种量为666.32 g、平均千粒重为532.35 g、平均出仁率为29.33%。选出的无性系可作为引种材料,进一步开展引种扩繁,为半干旱地区培育红松果林提供良种。