Inter Simple Sequence Repeat (ISSR) was used to detect genetic variation among nine provenances, including 135 individuals. A total of 108 loci were amplified using 10 random primers. The differentiation of the perc...Inter Simple Sequence Repeat (ISSR) was used to detect genetic variation among nine provenances, including 135 individuals. A total of 108 loci were amplified using 10 random primers. The differentiation of the percentage of polymorphic bands (PPB) among different provenances was evident, ranging from 27% to 54%. of which Honghuaerji provenance had the highest PPB and Kalunshan provenance had the lowest PPB. Shannon's Information index (1) at species level was 0.1581 and Nei's gene diversity (h) was 0.2393. Coefficient of gene differentiation (Gsr) calculated by Popgene was 0.3965, these results indicated that majority of genetic variation (60.35%) was found within provenances. According to dendrogram among pinus sylvestriv provenances, nine provenances were divided into two provenance areas. namely Daxing'an and Xiaoxing'an Mountains provenance area and Hulunbeier provenance area.展开更多
为进一步探明陕西省榆林市樟子松人工林的土壤养分变化特征,以5年生樟子松(Pinus sylvestris L. var. mongolica Litv.)纯林和5年生樟子松-胡枝子(Lespedeza bicolor Turcz.)混交林为研究对象,分析土壤有机质、硝态氮、有效磷、速效钾...为进一步探明陕西省榆林市樟子松人工林的土壤养分变化特征,以5年生樟子松(Pinus sylvestris L. var. mongolica Litv.)纯林和5年生樟子松-胡枝子(Lespedeza bicolor Turcz.)混交林为研究对象,分析土壤有机质、硝态氮、有效磷、速效钾等理化指标以及土壤酶活性的变化特征。结果表明,樟子松纯林和樟子松-胡枝子混交林土壤有机质、有效磷和速效钾含量整体上均随着土层深度的增加呈下降趋势,全磷含量均随着土层深度的增加呈先升高后降低的趋势,硝态氮含量则均随着土壤深度的增加而增加,5个指标均表现为樟子松-胡枝子混交林高于樟子松纯林,且樟子松-胡枝子混交林各层土壤有效磷含量均显著高于樟子松纯林(P<0.05);樟子松纯林和樟子松-胡枝子混交林土壤含水率各土层间差异均不显著,樟子松-胡枝子混交林5~15 cm土层土壤含水率含量显著高于樟子松纯林(P<0.05);樟子松纯林和樟子松-胡枝子混交林土壤碱性磷酸酶活性、过氧化氢酶活性、脲酶活性均随着土层深度的增加而降低,且樟子松-胡枝子混交林过氧化氢酶活性和脲酶活性在各土层均显著高于樟子松纯林(P<0.05)。展开更多
基金This study was supported by National Scientific and Technological Brainstorm Project.
文摘Inter Simple Sequence Repeat (ISSR) was used to detect genetic variation among nine provenances, including 135 individuals. A total of 108 loci were amplified using 10 random primers. The differentiation of the percentage of polymorphic bands (PPB) among different provenances was evident, ranging from 27% to 54%. of which Honghuaerji provenance had the highest PPB and Kalunshan provenance had the lowest PPB. Shannon's Information index (1) at species level was 0.1581 and Nei's gene diversity (h) was 0.2393. Coefficient of gene differentiation (Gsr) calculated by Popgene was 0.3965, these results indicated that majority of genetic variation (60.35%) was found within provenances. According to dendrogram among pinus sylvestriv provenances, nine provenances were divided into two provenance areas. namely Daxing'an and Xiaoxing'an Mountains provenance area and Hulunbeier provenance area.
文摘为进一步探明陕西省榆林市樟子松人工林的土壤养分变化特征,以5年生樟子松(Pinus sylvestris L. var. mongolica Litv.)纯林和5年生樟子松-胡枝子(Lespedeza bicolor Turcz.)混交林为研究对象,分析土壤有机质、硝态氮、有效磷、速效钾等理化指标以及土壤酶活性的变化特征。结果表明,樟子松纯林和樟子松-胡枝子混交林土壤有机质、有效磷和速效钾含量整体上均随着土层深度的增加呈下降趋势,全磷含量均随着土层深度的增加呈先升高后降低的趋势,硝态氮含量则均随着土壤深度的增加而增加,5个指标均表现为樟子松-胡枝子混交林高于樟子松纯林,且樟子松-胡枝子混交林各层土壤有效磷含量均显著高于樟子松纯林(P<0.05);樟子松纯林和樟子松-胡枝子混交林土壤含水率各土层间差异均不显著,樟子松-胡枝子混交林5~15 cm土层土壤含水率含量显著高于樟子松纯林(P<0.05);樟子松纯林和樟子松-胡枝子混交林土壤碱性磷酸酶活性、过氧化氢酶活性、脲酶活性均随着土层深度的增加而降低,且樟子松-胡枝子混交林过氧化氢酶活性和脲酶活性在各土层均显著高于樟子松纯林(P<0.05)。