The expression of transforming growth factor-β1 (TGF-β1) in placental tissue of pregnancy-induced hypertension (PIH) and the relationship between the level of expression of TGF-β1 and the amount of vascular cell ad...The expression of transforming growth factor-β1 (TGF-β1) in placental tissue of pregnancy-induced hypertension (PIH) and the relationship between the level of expression of TGF-β1 and the amount of vascular cell adhesion molecule-1 (VCAM-1) in serum was studied. Immunohistochemistry ABC was used to detect the expression and distribution of TGF-β1 in placental tissues in 40 PIH women and 20 normal pregnancy women. High resolution pathological image analysis system was used to determine the quality of TGF-β1. The VCAM-1 in serum was examined by enzyme linked immunoabsorbent assay (ELISA). The results showed that TGF-β1 could be express in syncytiotrophoblast. The levels of TGF-β1 expression in placental tissues of the patients with moderate and severe PIH were significantly higher (P<0.05), while the serum VCAM-1 was significantly lower than in normal group (P<0.01). There was a significant positive correlation between the expression of TGF-β1 in placental tissues and the serum VCAM-1 (r=0.969, P<0.01). It was concluded that the level of TGF-β1 expression in PIH was increased and was positively correlated with the amount of serum VCAM-1, indicating that they might be involved in the pathogenesis of PIH.展开更多
A heat\|stable growth factor has been purified to homogeneity from human placenta. Purification procedure involved extraction with acidic medium, precipitation using ethanol, DE\|52 ion\|exchange chromatography, and r...A heat\|stable growth factor has been purified to homogeneity from human placenta. Purification procedure involved extraction with acidic medium, precipitation using ethanol, DE\|52 ion\|exchange chromatography, and reversed\|phase HPLC. The purified preparation has been named human placental growth factor\|1 (HPGF\|1), which simulates the proliferation of some cell lines, such as human amniotic cell, mouse marrow tumor cell, and mouse fibroblast cell. The growth factor has an approximate molecular weight of 1 900. It is a glycopeptide which contains a polypeptide chain consisting of 10 amino acid residues with molecular weight of 1 195. The N\|terminal residue of the polypeptide chain part is phenylalanine. The molecular weight of non\|peptide part is 682. The isoelectric point of the growth factor is pI 6.8.展开更多
文摘The expression of transforming growth factor-β1 (TGF-β1) in placental tissue of pregnancy-induced hypertension (PIH) and the relationship between the level of expression of TGF-β1 and the amount of vascular cell adhesion molecule-1 (VCAM-1) in serum was studied. Immunohistochemistry ABC was used to detect the expression and distribution of TGF-β1 in placental tissues in 40 PIH women and 20 normal pregnancy women. High resolution pathological image analysis system was used to determine the quality of TGF-β1. The VCAM-1 in serum was examined by enzyme linked immunoabsorbent assay (ELISA). The results showed that TGF-β1 could be express in syncytiotrophoblast. The levels of TGF-β1 expression in placental tissues of the patients with moderate and severe PIH were significantly higher (P<0.05), while the serum VCAM-1 was significantly lower than in normal group (P<0.01). There was a significant positive correlation between the expression of TGF-β1 in placental tissues and the serum VCAM-1 (r=0.969, P<0.01). It was concluded that the level of TGF-β1 expression in PIH was increased and was positively correlated with the amount of serum VCAM-1, indicating that they might be involved in the pathogenesis of PIH.
文摘A heat\|stable growth factor has been purified to homogeneity from human placenta. Purification procedure involved extraction with acidic medium, precipitation using ethanol, DE\|52 ion\|exchange chromatography, and reversed\|phase HPLC. The purified preparation has been named human placental growth factor\|1 (HPGF\|1), which simulates the proliferation of some cell lines, such as human amniotic cell, mouse marrow tumor cell, and mouse fibroblast cell. The growth factor has an approximate molecular weight of 1 900. It is a glycopeptide which contains a polypeptide chain consisting of 10 amino acid residues with molecular weight of 1 195. The N\|terminal residue of the polypeptide chain part is phenylalanine. The molecular weight of non\|peptide part is 682. The isoelectric point of the growth factor is pI 6.8.
