Isolation and Purification of Plantamajoside and Acteoside from Plant Extract of Plantago asiatica L. by High Performance Centrifugal Partition Chromatography

Two phenylethanoid glycosides（PhGs）, plantamajoside and acteoside, were isolated and purified from the aerial parts ofPlantago asiatica for the first time by high performance centrifugal partition chromatography（HPCPC） with ethyl acetate, n-butanol, ethanol and water（0.5：0.5：0.1：1, volume ratio） as solvent system. A total of 45.6 mg of plantamajoside and 293.8 mg of acteoside were purified from 1341 mg of the n-butanol extract of P. asiatica, with a purity of 〉93.3% as determined by HPLC. The HPCPC fractions were analyzed by HPLC-DAD and the structures were identified by their retention time, UV, electrospray ionization multi stage tandem mass spectrometry（ESI-MSn） in the negative ion mode, and confirmed by NMR experiments. The characteristic fragment ions of ESI-MS of the two PhGs isolated from Plantago asiatica were discussed, which are specific and useful for the identification of the structures of PhGs.

车前草中苯乙醇苷类化合物的抗氧化活性研究

采用Folin-Ciocalteu法测定样品中的总多酚含量,并采用PCL和DPPH2种不同的抗氧化活性体外评价体系,对车前草80%乙醇提取物以及从中分离得到的3个苯乙醇苷单体化合物,麦角甾苷、异麦角甾苷和Plantamajo-side的抗氧化活性进行了系统研究。研究结果表明:车前草80%乙醇提取物、麦角甾苷、异麦角甾苷和Plantamajoside都具有良好的抗氧化活性。