期刊文献+
共找到21篇文章
< 1 2 >
每页显示 20 50 100
Overexpression of hepatic plasminogen activator inhibitor type 1 mRNA in rabbits with fatty liver 被引量:8
1
作者 Jian-Gao Fan~1 Liang-Hua Chen~2 Zheng-Jie Xu~1 Min-De Zeng~3 1 Department of Gastroenterology,Shanghai First People’s Hospital,Shanghai 200085,China2 Department of Cardiology,Shandong Provincial Hospital,Jinan 250021,China3 Shanghai Institute of Digestive Diseases,Shanghai 200080,China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2001年第5期710-712,共3页
INTRODUCTIONPlasminogen activator inhibitor type 1 ( PAI-I ), an approximately Mr 50000 glycoprotein, is the major physiological inhibitor of plasminogen activators. It is not only the priming factor for atheroscleros... INTRODUCTIONPlasminogen activator inhibitor type 1 ( PAI-I ), an approximately Mr 50000 glycoprotein, is the major physiological inhibitor of plasminogen activators. It is not only the priming factor for atherosclerosis and coronary thrombosis[1-3] , but also participates in the genesis of chronic hepatitis and liver fibrosis[4-11] . However, there has been no available report yet about the research of hepatic PAl-1 gene expression in hyperlipidemia and fatty liver. The present study aimed to explore the change of hepatic PAl-1 mRNA and its plasma activity by means of animal model. 展开更多
关键词 ANIMALS Fatty Liver Gene Expression HYPERLIPIDEMIA Liver Male plasminogen activator inhibitor 1 RNA Messenger RABBITS
下载PDF
Idiopathic pulmonary fibrosis in relation to gene polymorphisms of transforming growth factor-β1 and plasminogen activator inhibitor 1 被引量:7
2
作者 LI Xin-xia LI Ning +3 位作者 BAN Cheng-jun ZHU Min XIAO Bai DAI Hua-ping 《Chinese Medical Journal》 SCIE CAS CSCD 2011年第13期1923-1927,共5页
Background Idiopathic pulmonary fibrosis (IPF) is a progressive and lethal fibrotic lung disease of unknown etiology. Host susceptibility or genetic factors may be important for the predisposition to it. Transformin... Background Idiopathic pulmonary fibrosis (IPF) is a progressive and lethal fibrotic lung disease of unknown etiology. Host susceptibility or genetic factors may be important for the predisposition to it. Transforming growth factor-β1 (TGF-β1 a potent profibrotic cytokine) and plasminogen activator inhibitor 1 (PAl-1) play important roles in the development of pulmonary fibrosis. The objective of the study was to investigate the association between the gene polymorphisms of TGF-β1 869 T〉C and PAl-1 4G/5G and the susceptibility to IPF in Han ethnicity. Methods Polymerase chain reaction (PCR) and restriction fragment length polymorphism were performed to analyse the gene polymorphisms of TGF-β1 in 869T〉C and PAl-1 4G/5G in 85 IPF patients and 85 healthy controls matched in age, gender, race and smoker status. Results There was a significant difference in 869T〉C genotype distribution of TGF-β1 between IPF cases and controls, a significant negative association between TC genotype and the development of IPF (OR=0.508, 95% CI: 0.275-0.941) and a positive association between CC genotype and the development of IPF (OR=1.967, 95% CI: 1.063-3.641). There was a significant positive association between PAl-1 5G/5G genotype and the development of IPF (OR=0.418, 95% CI: 0.193-0.904). Conclusions Gene polymorphisms of TGF-β1 in 869T〉Cand PAl-1 4G/5G may affect the susceptibility to IPF in Han ethnicity. Further investigations are needed to confirm these findings and assess their biological significance in the development of the disease in this ethnic population. 展开更多
关键词 gene polymorphism genetic susceptibility idiopathic pulmonary fibrosis plasminogen activator inhibitor 1 transforming growth factor-beta 1
原文传递
Relationship of plasminogen activator inhibitor 1 gene 4G/5G polymorphisms to hypertension in Korean women 被引量:3
3
作者 Kyu-nam Kim Kwang-min Kim +3 位作者 Bom-taeck Kim Nam-seok Joo Doo-yeoun Cho Duck-joo Lee 《Chinese Medical Journal》 SCIE CAS CSCD 2012年第7期1249-1253,共5页
Background Hypertension (HTN) is a major determinant of various cardiovascular events. Plasma levels of plasminogen activator inhibitor 1 (PAl-l) modulate this risk. A deletion/insertion polymorphism within the PA... Background Hypertension (HTN) is a major determinant of various cardiovascular events. Plasma levels of plasminogen activator inhibitor 1 (PAl-l) modulate this risk. A deletion/insertion polymorphism within the PAl-1 loci (4G/4G, 4G/5G, 5G/5G) affects the expression of this gene. The present study investigated the association between PAl-1 loci polymorphisms and HTN in Korean women. Methods Korean women (n=1312) were enrolled in this study to evaluate the association between PAl-1 4G/5G gene polymorphisms and HTN as well as other metabolic risk factors. PAl-1 loci polymorphisms were investigated using polymerase chain reaction amplification and single-strand conformation polymorphism analysis. Results The three genotype groups differed with respect to systolic blood pressure (P=0.043), and diastolic blood pressure (P=0.009) but not with respect to age, body mass index, total cholesterol, low or high density lipoprotein cholesterol, triglycerides, or fasting blood glucose. Carriers of the PAl-1 4G allele had more hypertension significantly (PAl-1 4G/5G vs. PAl-1 5G/5G, P=0.032; PAl-1 4G/4G vs. PAl-1 5G/5G, P=0.034). When stratified according to PAl-1 4G/5G polymorphism, there was no significant difference in all metabolic parameters among PAl-1 genotype groups in patients with HTN as well as subjects with normal blood pressure. The estimated odds ratio of the 4G/4G genotype and 4G/5G for HTN was 1.7 (P=0.005), and 1.6 (P=0.015), respectively. Conclusion These findings might indicate that PAl-1 loci polymorphisms independently contribute to HTN and that gene-environmental interaction may be not associated in Korean women. 展开更多
关键词 HYPERTENSION plasminogen activator inhibitor 1 POLYMORPHISM
原文传递
THE INCREASE IN PLASMINOGEN ACTIVATOR INHIBITOR TYPE-1 EXPRESSION BY STIMULATION OF ACTIVATORS FOR PEROXISOME PROLIFERATOR-ACTIVATED RECEPTORS IN HUMAN ENDOTHELIAL CELLS 被引量:5
4
作者 叶平 胡晓晖 赵亚力 《Chinese Medical Sciences Journal》 CAS CSCD 2002年第2期112-116,共5页
Objective.To investigate the effect of peroxis ome proliferator-activated recept ors(PPARs )activators on plasminogen activator inhibitor ty pe-1(PAI-1)expression in human umbilical vein e ndothelial cells and the pos... Objective.To investigate the effect of peroxis ome proliferator-activated recept ors(PPARs )activators on plasminogen activator inhibitor ty pe-1(PAI-1)expression in human umbilical vein e ndothelial cells and the possi-ble mechanism.Methods.Human umbilical vein endothelial ce lls(HUVECs )were obtained from normal fetus,and cul-tured conventionally.Then the HUVECs were exposed to test agents(linolenic acid,linoleic acid,oleic acid,stearic acid and prostaglandin J 2 respectively)in varying concentrations with fresh media.RT -PCR and ELISA were applied to determine the expression of PPARs and PAI-1in HUVECs.Results.PPARα,PPARδand PPARγmRNA were detected by using RT-PCR in HUVECs.Treatment of HUVECs with PPARαand PPARγactivators---linolenic acid,linoleic acid,oleic acid and prostaglandin J 2 respectively,but not with stearic a cid could augment PAI-I mRNA expression and protein secretion in a concentration-dependent manner.However,the mRNA expressions of 3subclasses of PPAR with their activators in HUVECs were not changed compared w ith controls.Conclusion.HUVECs express PPARs.PPARs activators may increase PAI-1expression in ECs,but the underlying mechanism remains uncle ar.Although PPARs expression was not enhanced after stimulated by their activators in ECs,the role of functionally active PPARs in regulating PA I-1expression in ECs needs to be further investigated by using transient gen e transfection assay. 展开更多
关键词 peroxisome proliferator-activate d receptors plasminogen activator inhibitor type-1 EXPRESSION endothelial cells
下载PDF
Expression and prognostic value of plasminogen activator inhibitor type 1 in node-negative breast cancer 被引量:2
5
作者 Bin Wang Ning Wang +2 位作者 Chunyan Xue Bin Jiang Yajie Wang 《The Chinese-German Journal of Clinical Oncology》 CAS 2008年第6期339-343,共5页
Objective: To investigate the expressions of plasminogen activator inhibitor type 1 (PAl-1), C-erbB-2, VEGF and Ki-67 by immunohistostaining and then to evaluate the prognostic value of PAl-1 in node-negative breas... Objective: To investigate the expressions of plasminogen activator inhibitor type 1 (PAl-1), C-erbB-2, VEGF and Ki-67 by immunohistostaining and then to evaluate the prognostic value of PAl-1 in node-negative breast cancer. Methods: The study included a retrospective series of 62 female patients with axillary lymph node-negative breast cancer. Expressions of PAl-1, C-erbB-2, VEGF and Ki-67 were determined by immunohistostaining on formalin-fixed paraffin-embedded tissue sections from these patients after a median follow-up of 69 months (range 22-117 months). Correlations with well known clinicopathologic factors were assessed and multivariate survival analyses were performed. Results: High PAl-1 level was positively associated with high histologic grade of the tumors. Disease-free survival (DFS) was significantly shorter for the patients with moderate to intensive expression of PAl-1 than for those with negative (χ^2 = 25.46, P 〈 0.001; χ^2 = 23.07, P 〈 0.001) to mild expression (χ^2 = 19.75, P 〈 0.001; χ^2 = 17.40, P 〈 0.001). Although on univariate analysis of the prognostic factors, tumor size, location of primary tumor and age as well as expressions of PAl-1, VEGF and Ki-67 were all significantly prognostic factors for DFS (P 〈 0.05), PAl-1 was the only independent prognostic factor on multivariate analysis (P 〈 0.0001; hazard ratio [HR], 4.041; 95% confidence interval [CI], 1.928-8.468). Conclusion: These results of the current study indicate that intermediate or high expression of PAl-1 represents a strong and independent unfavorable prognostic factor for the development of recurrence or metastases in axillary node-negative breast cancer. 展开更多
关键词 breast carcinoma NODE-NEGATIVE plasminogen activator inhibitor type 1 (PAl-1 PROGNOSIS
下载PDF
EFFECTS OF TGF-β_1 ON THE EXPRESSION OF PLASMINOGEN ACTIVATOR INHIBITOR TYPE 1 IN CULTURED HUMAN RENAL INTERSTITIAL FIBROBLASTS
6
作者 王伟铭 姚建 +3 位作者 石蓉 周同 陈楠 董德长 《Medical Bulletin of Shanghai Jiaotong University》 CAS 2000年第2期77-79,共3页
Objective To investigate the effects of transforming growth factor-β1 (TGF-β1 ) on the expression of plasminogen activator inhibitor type 1 (PAI-1 ) mRNA in renal interstitial fibrosis in vitro. Methods Human renal ... Objective To investigate the effects of transforming growth factor-β1 (TGF-β1 ) on the expression of plasminogen activator inhibitor type 1 (PAI-1 ) mRNA in renal interstitial fibrosis in vitro. Methods Human renal interstitial fibroblasts were isolated and cultured in vitro. The cells wers stimulated by TGF-β1 with different concentration (0 to 10ng/ml ) at different time (0 to 48h). The expression of PAI-1 mRNA was assayed by RT-PCR. Results TGF-β1, had dose-dependent and time-dependent effects on the expression of PAI-1 mRNA in renal interstitial fibroblasts. Conclusion TGF-β1 may partic- ipate in renal fibrosis with inducing the expression of PAI-1 mRNA in renal fibroblasts and affecting the synthesis and degradation of extracellular matrix (ECM). 展开更多
关键词 transforming growth factor-β1 renal interstitial fibroblasts plasminogen activator inhibitor type 1
下载PDF
tPA Involvement in Ovulation──Studies on Mechanism of Ovulation:Role of Tissue Type Plasminogen Activator
7
作者 LIU Yi-xun(State Key Laboratory of Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences, Beijing 100080) 《Developmental and Reproductive Biology》 1994年第2期70-78,共9页
This review summarized our recent studies on involvement of tissue type plasminogen activator(tPA)and plasminogen activator inhibitor type 1(PAI-1) in process of ovulation.We have demonstrated that 1)hCG induces ovula... This review summarized our recent studies on involvement of tissue type plasminogen activator(tPA)and plasminogen activator inhibitor type 1(PAI-1) in process of ovulation.We have demonstrated that 1)hCG induces ovulation and coordinated tPA and PAI-1 gene expression in both rat and monkey ovaries;(2) GnRH and FSH are also capable of inducing ovulation by increasing ovarian tPA and PAI-1 gene expression in the same manner as hCG does;(3)Compounds which increase tPA production can induce oviation while compounds which decrease tPA and/or increase PAI-1 expression inhibit ovulation. Based on the data provided,a working model on the involvement of tPA in ovulation is presented. 展开更多
关键词 Tissue type plasminogen activator(tPA) plasminogen activator inhibitor type 1 (pAI-1) OVULATION
下载PDF
The involvement of p38 MAPK in transforming growth factor β1-induced apoptosis in murine hepatocytes 被引量:15
8
作者 LiaoJH ChenJS 《Cell Research》 SCIE CAS CSCD 2001年第2期89-94,共6页
We reported in this manuscript that TGF-beta1 induces apoptosis in AML12 murine hepatocytes, which is associated with the activation of p38 MAPK signaling pathway. SB202190, a specific inhibitor of p38 MAPK, strongly ... We reported in this manuscript that TGF-beta1 induces apoptosis in AML12 murine hepatocytes, which is associated with the activation of p38 MAPK signaling pathway. SB202190, a specific inhibitor of p38 MAPK, strongly inhibited the TGF-beta1-induced apoptosis and PAI-1 promoter activity. Treatment of cells with TGF-beta1 activates p38. Furthermore, over-expression of dominant negative mutant p38 also reduced the TGF-beta1-induced apoptosis. The data indicate that the activation of p38 is involved in TGF-beta1-mediated gene expression and apoptosis. 展开更多
关键词 Animals Apoptosis Cells Cultured DNA Fragmentation Enzyme inhibitors Gene Expression Regulation Enzymologic Genes Reporter Genetic Vectors HEPATOCYTES IMIDAZOLES MAP Kinase Signaling System Mice Mitogen-Activated Protein Kinases Mutation Phosphorylation plasminogen activator inhibitor 1 PYRIDINES Research Support Non-U.S. Gov't TRANSFECTION Transforming Growth Factor beta p38 Mitogen-Activated Protein Kinases
下载PDF
Perilla oil and exercise decrease expressions of tumor necrosis factor-α,plasminogen activator inhibitor-1 and highly sensitive C-reactive protein in patients with hyperlipidemia 被引量:10
9
作者 Minggang Wei Peihua Xiong +3 位作者 Ling Zhang Mei Fei Aiping Chen Fengling Li 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2013年第2期170-175,共6页
OBJECTIVE:To verify the effects of perilla oil on the regulation of blood lipid levels in patients with hyperlipidemia.METHODS:Blood was taken from patients prior to and 8 weeks following treatment with perilla oil.Di... OBJECTIVE:To verify the effects of perilla oil on the regulation of blood lipid levels in patients with hyperlipidemia.METHODS:Blood was taken from patients prior to and 8 weeks following treatment with perilla oil.Different ways to test for indexes which correlate to hyperlipidemia were performed.Some indexes,which correlate with inflammation and injury to endothelial cells,were tested using enzyme linked immunosorbent assays.RESULTS:Serum lipid levels [triglyceride(TG),total cholesterol(TC),and low-density lipoprotein-cholesterol(LDL-C)] changed significantly after 56 days of treatment.Differences were noted as early as 28 days after treatment began(P<0.05).Treatment with perilla oil showed statistically significant recovery levels of high-density lipoprotein-cholesterol(HDL-C) after 28 and 56 days of treatment.Plasma lipids levels were significantly lower after 56 days of treatment(P<0.05).Perilla oil reduced blood lipid levels in patients,and the regulation of cell signaling factor levels had no adverse effects on patients' liver or kidney function,or blood routine examinations.CONCLUSION:Perilla oil treatment is safe in clinical use,can regulate blood lipid levels and protects the function of endothelial cells. 展开更多
关键词 Fructus perillae Tumor necrosis fac-tor-alpha plasminogen activator inhibitor 1 C-reac-tive protein HYPERLIPIDEMIAS
原文传递
Activation of peroxisome proliferator-activated receptor α in human endothelial cells increases plasminogen activator inhibitor type-1 expression 被引量:7
10
作者 叶平 胡晓晖 +1 位作者 刘永学 赵亚力 《Chinese Medical Journal》 SCIE CAS CSCD 2003年第1期29-33,共5页
Objective To investigate the effect of peroxisome proliferator-activated receptors (PPARs) activators on plasminogen activator inhibitor 1 (PAI-1) expression in human umbilical vein endothelial cells and elucidate a ... Objective To investigate the effect of peroxisome proliferator-activated receptors (PPARs) activators on plasminogen activator inhibitor 1 (PAI-1) expression in human umbilical vein endothelial cells and elucidate a possible mechanism. Methods Human umbilical vein endothelial cells (HUVECs) were obtained from normal fetus, and cultured conventionally. Then the HUVEC were exposed to fatty acids and prostaglandin J 2 in varying concentrations with fresh media. RT-PCR and ELISA were used to determine the expression of PPAR and PAI-1 in HUVECs. Transient co-transfection of PAI-1 promoter and PPARα gene or PPARγ gene to ECV304 was performed.Results PPARα, PPARδ and PPARγ mRNA in HUVECs were detected by RT-PCR. Treatment of HUVECs with PPARα and PPARγ activators-linolenic acid, linoleic acid, oleic acid and prostaglandin J 2, but not with stearic acid could augment PAI-I mRNA expression and protein secretion in a concentration-dependent manner. Proportional induction of PAI-1 promoter activity was observed through increasing amounts of PPARα DNA in HUVECs through a transient gene transfection assay, although the mRNA expression of the 3 subtypes of PPAR with their activators were not changed compared with controls.Conclusions HUVECs express PPARs. PPARs activators may increase PAI-1 expression in endothelial cells (EC). Although PPARs expression was not enhanced after being stimulated by their activators in EC, the functionally active PPARα is probably involved in regulating PAI-1 expression in EC. 展开更多
关键词 peroxisome proliferator-activated receptors plasminogen activator inhibitor 1 umbilical veins
原文传递
Plasminogen activator inhibitor-1 4G/5G gene polymorphism in patients with myocardial or cerebrovascular infarction in Tianjin, China 被引量:9
11
作者 战梅 周玉玲 韩忠朝 《Chinese Medical Journal》 SCIE CAS CSCD 2003年第11期1707-1710,共4页
Objective To investigate the association between the plasminogen activator inhibitor-1 (PAI-1) 4G/ 5G gene polymorphism and the occurrence of myocardial and cerebrovascular infarctions in individuals from Tianjin, Chi... Objective To investigate the association between the plasminogen activator inhibitor-1 (PAI-1) 4G/ 5G gene polymorphism and the occurrence of myocardial and cerebrovascular infarctions in individuals from Tianjin, China.Methods The PAI-1 genotype was determined using allele-specific polymerase chain reaction (AS-PCR) in 56 myocardial infarction (Ml) patients, 54 cerebrovascular infarction (Cl) patients and 83 unrelated healthy controls. All subjects' clinical features and plasma PAI-1 activity levels were determined.Results The PAI-1 genotype distribution frequency of the single guanine deletion/insertion 4G/5G polymorphism (located -675 bp upstream from the start of transcription) significantly differed between the patients and healthy controls. In the Ml group, the4G/4G-genotype frequency was increased, but the 4G/5G-genotype is decreased when compared to the control group. In the Cl group, both the 4G/ 4G- and 4G/5G -genotypes occured at a lower frequency than those in the control group (P<0. 001) . The plasma PAI-1 activity level in the Ml group was lowered as the presence of the 4G allele decreases. In the Cl group, the frequency of 5G/5G was much higher than that of the control group (P<0. 001). The plasma PAI-1 activity level in the Cl group was elevated as the presence of the 5G allele increased. Furthermore, positive correlation between triglyceride, glucose levels and PAI-1 activity were found in all three groups (P<0. 001).Conclusions The PAI-1 4G/5G gene polymorphism is associated with a higher risk of Ml and Cl in individuals in Tianjin, China. The deletion/insertion polymorphism is probably an important hereditary risk factor for heart diseases. Moreover, triglyceride and glucose levels of plasma have functional importance in regulating PAI-1 activity. 展开更多
关键词 plasminogen activator inhibitor 1 · polymorphism ·myocardial infarction · cerebral infarction
原文传递
Gene expression of fibrinolytic factors urokinase plasminogen activator and plasminogen activator inhibitor-1 in rabbit temporo-mandibular joint cartilage with disc displacement 被引量:3
12
作者 ZHANJing GUZhi-yuan +2 位作者 WULi-qun ZHANGYin-kai HUJi-an 《Chinese Medical Journal》 SCIE CAS CSCD 2005年第12期1000-1006,共7页
Background The urokinase plasminogen activator system is believed to play an important role in degradation of the extracellular matrix associated with cartilage and bone destruction; however its precise roles in temp... Background The urokinase plasminogen activator system is believed to play an important role in degradation of the extracellular matrix associated with cartilage and bone destruction; however its precise roles in temporomandibular disorders have not yet been clarified. The aims of this study were to investigate the gene expression of fibrinolytic factors urokinase plasminogen activator (uPA) and plasminogen activator inhibitor-1 (PAI-1) in the articular cartilage of rabbit temporomandibular joint (TMJ) with disc displacement (DD) and to probe the relationship between fibrinolytic activity and cartilage remodeling. Methods Disc displacement of right joints was performed in 36 of 78 rabbits under investigation. The animals were sacrificed at 4 days and 1, 2, 4, 8 and 12 weeks after surgery, respectively. The right joints of these animals were harvested and processed for the examination of mRNA expression of uPA and PAI-1 in articular cartilage using in situ hybridization techniques. Results The expression of uPA and PAI-1 was co-expressed weakly in the chondrocytes from transitive zone to hypertrophic zone and mineralized zone, while no hybridizing signals were shown in proliferative zone and superficial zone in control rabbits. The most striking was the up-regulation of uPA and PAI-1 mRNA in 4-day rabbits postoperatively at the onset of cartilage degeneration. The strongest hybridizing signals for uPA and PAI-1 were seen in 2-week rabbits postoperatively. After 2 weeks, the expression of uPA and PAI-1 began to decrease and reached nearly normal level at 12 weeks. Conclusions The expression of the uPA/PAI-1 system coincides with the pathological changes in condylar cartilage after DD. The uPA/PAI-1 system may be one of the essential mediators in articular cartilage remodeling. 展开更多
关键词 urokinase plasminogen activator · plasminogen activator inhibitor 1 · temporomandibular joint · disc displacement · in situ hybridization
原文传递
Tissue-type plasminogen activator and plasminogen activator inhibitor type-1 mRNA and their protein expression levels in human decidua after early pregnancy termination by mifepristone plus misoprostol
13
作者 黄丽丽 石一复 《Chinese Medical Journal》 SCIE CAS CSCD 2001年第6期68-71,108,共5页
Objective To investigate the mechanism of prolonged uterine hemorrhage after terminating early pregnancy by mifepristone plus misoprostol.Methods Forty-five decidua specimens were obtained from 45 pregnant women wit... Objective To investigate the mechanism of prolonged uterine hemorrhage after terminating early pregnancy by mifepristone plus misoprostol.Methods Forty-five decidua specimens were obtained from 45 pregnant women with amenorrhea of 6-7 week duration. Fifteen women were treated with mifepristone and 15 were treated with mifepristone plus misoprostol. The remaining 15 served as controls. The tPA and PAI-1 mRNA levels were estimated by reverse transcription-polymerase chain reaction. Chromogenic assay and enzyme-linked immunosorbent assay were used to detect tPA activity and PAI-1 protein level in decidua. Results The activities of tPA in the mifepristone plus misoprostol group and in the mifepristone group were 46.91±20.74?IU/mg*protein and 64.25±35.81?IU/mg*protein respectively, lower than those in the normal decidua group (99.76±58.61?IU/mg*protein, P<0.05). tPA mRNA levels in the mifepristone plus misoprostol group were the highest (1.43±0.39) among the groups. In the mifepristone group, tPA mRNA level (0.90±0.16) was not significantly different from that in the normal decidua group (0.94±0.17). The protein and mRNA expression levels of PAI-1 were not significantly different among the three groups (P>0.05).Conclusions Mifepristone plus misoprostol decreased tPA activity in human early decidua by post-transcription pathways, which may influence decidua shedding, endometrial angiogenesis, endometrial remodeling, and cause prolonged uterine hemorrhage after drug abortion. 展开更多
关键词 tissue-type plasminogen activator · plasminogen activator inhibitor type-1 · mifepristone · decidua · uterine hemorrhage
原文传递
Evaluate the Expression of uPA,PAI-1 in Human Gastric Cancer and its Correlation with the Angiogenesis by the Application of Tissue Microarray
14
作者 Jifeng Wu Xia Sheng +1 位作者 Rong Qin Hong Zhang 《Clinical oncology and cancer researeh》 CAS CSCD 2009年第3期186-191,共6页
OBJECTIVE To investigate the expression of urokinase-type plasminogen (uPA), its inhibitor-1 (PAI-1) mRNA and its protein in human gastric cancer and to find out the relationship among the tumor differentiation, a... OBJECTIVE To investigate the expression of urokinase-type plasminogen (uPA), its inhibitor-1 (PAI-1) mRNA and its protein in human gastric cancer and to find out the relationship among the tumor differentiation, angiogenesis, and other clinical pathologic factors. METHODS In situ hybridization (ISH) was used to get the uPA, PAI-lmRNA in 110 cases with human gastric cancer in 2-tissue microarray (TMA). Immunohistochemical staining (S-P method) for uPA, PAI-1 protein and CD34 were performed in the 110 cases in 2 TMA. RESULTS The expression of the uPA, PAI-lmRNA and their protein happened in the cytoplasm of gastric cancer cells were induced by the poor differentiation of the GC, and the expression of uPA had an increasing trend while the expression of the PAI-1 had a decreasing trend. The microvessel density (MVD) had a positive correlation with the clinical stages and the significant relationship with the lymph node metastasis (P 〈 0.05). The MVD in uPA positive group was significantly higher than those in uPA negative group (P 〈 0.05). The expression of PAI-1 has no correlation neither with the clinical stages nor the lymph node metastasis. CONCLUSION The uPA play an important role in invasion and metastasis of GC through promoting angiogenesis. Interdicting the secretion and function of the uPA may allow the target therapy against the tumor invasion. As a new high-throughput technology, the tissue microarray is a valuable way to be used in clinical treatment. 展开更多
关键词 stomach neoplasmas urinary plasminogen activator plasminogen activator inhibitor 1 ANGIOGENESIS tissue microarray.
下载PDF
Alterations in fibrinolytic system proteins PAI-1,MMP-3,MMP-8,TIMP-1 and TIMP-2 in post-cholecystectomy bile duct injury
15
作者 Jose Manuel Hermosillo-Sandoval Luis Miguel Román-Pintos +4 位作者 Adolfo Daniel Rodriguez-Carrizález Ernesto Germán Cardona-Munoz Fermin Paul Pacheco-Moisés Genaro Gabriel Ortiz Alejandra Guillermina Miranda-Diaz 《Journal of Biomedical Science and Engineering》 2013年第8期58-67,共10页
Introduction: In bile duct injuries (BDI), cholestasis and cholangitis can alter the fibrinolytic system by promoting an increase of extracellular matrix depositions which favor an imbalance between metalloproteinases... Introduction: In bile duct injuries (BDI), cholestasis and cholangitis can alter the fibrinolytic system by promoting an increase of extracellular matrix depositions which favor an imbalance between metalloproteinases (MMPs) and their tissue inhibitors (TIMPs). Materials and Methods: Levels of PAI-1, MMP-3, MMP-8, TIMP-1 and TIMP-2 in 35 patients with post-cholecystectomy BDI by complete biliary obstruction were measured and compared to a healthy control group. Sirius red staining and immune staining for MMP-3 and MMP-8 were also undertaken in liver biopsies. Results: Levels of PAI-1, TIMP-1, TIMP-2 and MMP-8 were higher in BDI than healthy controls: 15 ± 2 ng/mL vs 7.1 ± 2 ng/mL (p 0.024);539 ± 64 ng/mL vs 256 ± 13 ng/mL (p p p 2 vs. 22865.7 ± 3865 μm2 in healthy controls (p 2 vs. 30744.2 ± 5810.2 μm2 (p 2 vs. 116337.9 ± 24803.3 μm2 (p 0.55). These results suggest an imbalance between fibrogenic/fibrinolytic protein levels. Interestingly, expression of the fibrinolytic protein MMP-8 was increased in serum and liver biopsies in BDI. Conclusion: We found an imbalance of profibrogenic molecules which promote extracellular matrix deposition. The over-expression of fibrinolytic proteins such as MMP-8 could limit liver fibrosis, preventing hepatic dysfunction in post-cholecystectomy BDI. 展开更多
关键词 CHOLECYSTECTOMY Bile Duct Injury(BDI) plasminogen activator inhibitor Type 1(PAI-1) Tissue inhibitors of Metalloproteinases(TIMP’s) Metalloproteinases(MMP’s)
下载PDF
The Physiopathological Crossroads of Aging
16
作者 J.Lasierra-Cirujeda P.Coronel +4 位作者 M.J.aza pascual-salcedo M.Gimeno M.M.Aza Pascual Salcedo A.Lasierra-Ibanez C.Lasala-Aza 《Journal of Biosciences and Medicines》 2019年第6期102-128,共27页
Stress, inflammation and Plasminogen activator inhibitor 1 (PAI-1) are key mechanisms throughout the development of aging, constituting a crossroad in the most frequent pathologies that accompany it. Among metabolic p... Stress, inflammation and Plasminogen activator inhibitor 1 (PAI-1) are key mechanisms throughout the development of aging, constituting a crossroad in the most frequent pathologies that accompany it. Among metabolic processes, obesity, metabolic syndrome and type 2 diabetes mellitus are included and Alzheimer’s disease among the neurodegenerative processes. Stress is a mechanism of defense of the organism against exogenous and endogenous actions called stressors. In the case of low intensity stimuli, the organism responds with actions aimed at a physiological adaptation (Homeostasis). On the other hand, when a high intensity (experimental level) or chronic stimulus (oxidative stress) is repeated, structural and functional changes are observed in different organs with activation of the hypothalamus-pituitary-adrenal axis, the renin angiotensin system and the sympathetic nervous system, stimulating the production of hormones that release cytokines with proin-flammatory/antiinflammatory properties that play an important role in the previously mentioned pathologies, as well as a marked increase in PAI-1, a gene regulated by stress and by cytokines, with manifest action at the origin of thromboembolic disease, so frequent in aging. The objective of this review is to highlight the importance of the binomial stress and PAI-1 in aging and in the pathologies that accompany it. Because PAI-1 is part of the pathology and complications in aging, some authors suggest the study of PAI-1 inhibitors to achieve its physiological levels, as part of the treatment of these diseases. 展开更多
关键词 Aging. Oxidative Stress plasminogen activator inhibitor 1 Transforming Growth Factor Beta 1 GLUTATHIONE Alzheimer’s Disease
下载PDF
Relationship between gene polymorphism of the PAI-1 promoter and myocardial infarction 被引量:3
17
作者 富路 金红 +3 位作者 宋克宁 张翠丽 沈景霞 黄永麟 《Chinese Medical Journal》 SCIE CAS CSCD 2001年第3期42-45,105-106,共6页
abstractObjective To investigate the association between gene polymorphism of the plasminogen activator inhibitor 1 (PAI 1) and myocardial infarction (MI) in Chinese Methods PAI 1 genotyping with polymerase chai... abstractObjective To investigate the association between gene polymorphism of the plasminogen activator inhibitor 1 (PAI 1) and myocardial infarction (MI) in Chinese Methods PAI 1 genotyping with polymerase chain reaction restriction fragment length polymorphism (PCR RFLP) and allele specific polymerase chain reaction (ASPCR) was performed in 87 myocardial infarction patients and 92 unrelated healthy controls All subjects'clinical features and PAI 1 activity were tested Results There were two polymorphisms within the promoter, a G/A single base substitution polymorphism upstream at -844?bp, and a single guanosine deletion/insertion 4G/5G polymorphism -675?bp upstream from the start of transcription Significant differences between the patients and the controls were observed neither for the frequencies of the GG, GA and AA genotypes nor for the PAI 1 activities of these three types But for the 4G/5G polymorphism, there were significant differences between patients and controls for the frequencies of the 4G/4G, 4G/5G and 5G/5G genotypes ( P <0 05) In the MI group, the PAI 1 activity of the 4G/4G type was significantly higher than that of the 5G/5G type ( P <0 05) Further more, a positive correlation between the glucose level and PAI 1 activity was found ( r =0 34, P =0 02) Conclusion This study indicates that the 4G/5G gene polymorphism of PAI 1 is associated with myocardial infarction, that 4G/4G type is probably an important hereditary risk factor, and that glucose has functional importance in regulating PAI 1 activity 展开更多
关键词 plasminogen activator inhibitor 1 · gene polymorphism · myocardial infarction
原文传递
Effects of Modified Qing'e Pill(加味青娥丸) on Expression of Adiponectin,Bone Morphogenetic Protein 2 and Coagulation-Related Factors in Patients with Nontraumatic Osteonecrosis of Femoral Head 被引量:7
18
作者 LI Cheng-gang SHEN Lin +3 位作者 YANG Yan-Ping XU Xiao-Juan SHUAI Bo MA Chen 《Chinese Journal of Integrative Medicine》 SCIE CAS CSCD 2017年第3期183-189,共7页
Objective: To observe the regulation of Chinese herbal medicine, Modified Qing'e Pill(加味青娥丸, MQEP), on the expression of adiponectin, bone morphogenetic protein 2(BMP2), osteoprotegerin(OPG) and other pot... Objective: To observe the regulation of Chinese herbal medicine, Modified Qing'e Pill(加味青娥丸, MQEP), on the expression of adiponectin, bone morphogenetic protein 2(BMP2), osteoprotegerin(OPG) and other potentially relevant risk factors in patients with nontraumatic osteonecrosis of the femoral head(ONFH). Methods: A total of 96 patients with nontraumatic ONFH were unequal randomly divided into treatment group(60 cases) and control group(36 cases). The treatment group were treated with MQEP while the control group were treated with simulated pills. Both groups were given caltrate D. Six months were taken as a treatment course. Patients were followed up every 2 months. The levels of plasma adiponectin, BMP2, OPG, von Willebrand factor(vWF), von Willebrand factor cleaving protease(vWF-cp), plasminogen activator inhibitor 1(PAI-1), tissue plasminogen activator(tPA), C-reactive protein(CRP), blood rheology, bone mineral density(BMD) of the femoral head and Harris Hip Score were measured before and after treatment. Results: After 6 months of treatment, compared with the control group, patients in the treatment group had significantly higher adiponectin and BMP2 levels(P〈0.01 and P=0.013, respectively), lower vWF, PAI-1 and CRP levels(P=0.019, P〈0.01 and P〈0.01, respectively), and lower blood rheology parameters. BMD of the femoral neck, triangle area and Harris Hip Score in the treatment group were significantly higher than those in the control group. Moreover, plasma adiponectin showed a positive association with BMP2(r=0.231, P=0.003) and a negative association with PAI-1(r=–0.159, P〈0.05). Conclusions: MQEP may play a protective role against nontraumatic ONFH by increasing the expression of adiponectin, regulating bone metabolism and improving the hypercoagulation state, which may provide an experimental base for its clinical effects. 展开更多
关键词 osteonecrosis femoral head Chinese herbal medicine adiponectin bone morphogenetic protein 2 plasminogen activator inhibitor 1
原文传递
Effects of pioglitazone on arteriosclerotic-related factors and short term prognosis in patients with coronary disease and diabetes
19
作者 梅百强 梁茜 +2 位作者 杨希立 区展鹏 文永钊 《South China Journal of Cardiology》 CAS 2011年第4期221-226,共6页
Background Thiazolidinediones (TZDs) not only improve insulin resistance, lowering blood sugar, also has anti-atherosclerotic effect. However, whether the protective effect on cardiovascular pioglitazone is still co... Background Thiazolidinediones (TZDs) not only improve insulin resistance, lowering blood sugar, also has anti-atherosclerotic effect. However, whether the protective effect on cardiovascular pioglitazone is still controversial. Methods Totally 98 patients with coronary disease and diabetes mellitus were randomly divided into pioglitazone group (n = 48) receiving conventional therapy and pioglitazone (15 mg/day), and control group (n = 50) merely receiving conventional therapy. The patients were followed up for 12 months. The plasma level of Plasminogen activator Inhibitor 1 (PAI-1) and P-selectin were detected at baseline and after treatment for 12 months by ELISA, and major adverse cardiac events (MACE) were studied. Results Pioglitazone therapy for 12 months was associated with a significant decrease of PAI-1 [(7.9 ± 1.4 vs 4.2 ± 0.5)ng/mL, P 〈 0.05] and P-selectin [(16.6 ± 6.8 vs 12.4 ± 3.6)ng/mL, P 〈 0.05], MACE was significantly lower in the pioglitazone group than in the control group [acute coronary syndrome (ACS): 32.0% vs 10.4%, P 〈 0.05; target vessel revascularization: 22.0% vs 6.3%, P 〈 0.05 ]. Conclusions Pioglitazone can effectively reduce the plasma level of PAI-1, P-selectin and the occurrence of MACE in patients with coronary heart disease and diabetes mellitus. 展开更多
关键词 PIOGLITAZONE plasminogen activator inhibitor 1 P-SELECTIN coronary disease diabetes mellitus
原文传递
Clinical and genetic risk factors for venous thromboembolismin Chinese population
20
作者 Chen WANG Zhen-Guo ZHAI +1 位作者 Ying H.SHEN Lan ZHAO 《Frontiers of Medicine》 SCIE CSCD 2010年第1期29-35,共7页
Venous thromboembolism(VTE),including deep vein thrombosis and pulmonary embolism,carries significant mortality and morbidity.The most important and effective way to reduce VTE incidence is to identify the patients at ... Venous thromboembolism(VTE),including deep vein thrombosis and pulmonary embolism,carries significant mortality and morbidity.The most important and effective way to reduce VTE incidence is to identify the patients at risk and give necessary prevention.VTE is a multifactorial and complicated disorder.Major risk factors for VTE include surgery and trauma,acute medical illness,active cancer and pregnancy.Genetic factors increase susceptibility to the disease and are useful in predicting the development of VTE.Gene-gene and gene-environment interactions alter and magnify the clinical picture in this disorder.This brief review summarizes some selected clinical and genetic risk factors for VTE based on the current research in China. 展开更多
关键词 risk factor STROKE PROTHROMBIN plasminogen activator inhibitor type-1 POLYMORPHISM THROMBOPHILIA BIOMARKER
原文传递
上一页 1 2 下一页 到第
使用帮助 返回顶部